Reviewed, UniProtKB/Swiss-Prot O04354 (CYB5_BOROF)

Names and origin

Protein names	Recommended name:     Cytochrome b5
Organism	Borago officinalis (Bourrache) (Borage)
Taxonomic identifier	13363 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Boraginaceae › Borago

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	Cytochrome b5		PRO_0000166022
Regions
	Transmembrane	104 – 124	21	Potential
	Domain	2 – 78	77	Cytochrome b5 heme-binding
Sites
	Metal binding	37	1	Iron (heme axial ligand) By similarity
	Metal binding	61	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04354-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 96EF72A06F2E8C5B	FASTA	132	14,557
	10 20 30 40 50 60 MGKIFTLAEV AQHNNSKDCW LIINGKVYDV TKFLEDHPGG DDVLLSATGK DATDDFEDIG 70 80 90 100 110 120 HSSSAKAMLD EYYVGDIDSS SIPSQVKYTP PKQPLYNPDK TREFVIKLLQ FLVPLVILAG 130 AIGIRFYTKS SA

References

[1]

"Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco." Sayanova O., Smith M.A., Lapinskas P.A., Stobart K., Dobson G., Christie W.W., Shewry P.R., Napier J.A. Proc. Natl. Acad. Sci. U.S.A. 94:4211-4216(1997) [PubMed: 9108131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U79011 mRNA. Translation: AAC49701.1.
3D structure databases
HSSP	HSSP built from PDB template 1CYO based on UniProtKB P00171.
ModBase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5_BOROF

Accession

Primary (citable) accession number: O04354

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)