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Protein

Thylakoidal processing peptidase 1, chloroplastic

Gene

TPP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves the thylakoid-transfer domain from a chloroplast protein.1 Publication

Catalytic activityi

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei184 – 1841By similarity

GO - Molecular functioni

  • endopeptidase activity Source: TAIR
  • serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

  • signal peptide processing Source: TAIR
  • thylakoid membrane organization Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT2G30440-MONOMER.

Protein family/group databases

MEROPSiS26.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Thylakoidal processing peptidase 1, chloroplastic (EC:3.4.21.89)
Alternative name(s):
Signal peptidase I-1
Gene namesi
Name:TPP1
Ordered Locus Names:At2g30440
ORF Names:T6B20.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30440.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei155 – 17521HelicalSequence analysisAdd
BLAST
Topological domaini176 – 340165Lumenal, thylakoidSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 340Thylakoidal processing peptidase 1, chloroplasticPRO_0000310734
Transit peptidei1 – 5252ChloroplastSequence analysisAdd
BLAST
Transit peptidei53 – ?ThylakoidSequence analysis

Proteomic databases

PaxDbiO04348.
PRIDEiO04348.

Expressioni

Gene expression databases

GenevisibleiO04348. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G30440.1.

Structurei

3D structure databases

ProteinModelPortaliO04348.
SMRiO04348. Positions 180-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi131 – 1355Poly-Asp

Sequence similaritiesi

Belongs to the peptidase S26 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0171. Eukaryota.
COG0681. LUCA.
HOGENOMiHOG000243179.
InParanoidiO04348.
KOiK03100.
OMAiDTIYHDQ.
PhylomeDBiO04348.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR015927. Peptidase_S24_S26A/B/C.
IPR019533. Peptidase_S26.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF10502. Peptidase_S26. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIRITFTYS THVARNLVGT RVGPGGYCFE SLVRPRFFSH KRDFDRSPRN
60 70 80 90 100
RPASMYGSIA RELIGEGSQS PLVMGLISIL KSTTGHESST MNVLGVSSFK
110 120 130 140 150
ASSIIPFLQG SKWIKNPPVI DDVDKGGTVC DDDDDKESRN GGSGWVNKLL
160 170 180 190 200
SVCSEDAKAA FTAVTVSILF RSALAEPKSI PSTSMYPTLD KGDRVMAEKV
210 220 230 240 250
SYFFRKPEVS DIVIFKAPPI LLEYPEYGYS SNDVFIKRIV ASEGDWVEVR
260 270 280 290 300
DGKLFVNDIV QEEDFVLEPM SYEMEPMFVP KGYVFVLGDN RNKSFDSHNW
310 320 330 340
GPLPIENIVG RSVFRYWPPS KVSDTIYHDQ AITRGPVAVS
Length:340
Mass (Da):37,853
Last modified:November 1, 1998 - v2
Checksum:iF6763EBFC1DAA2E1
GO

Sequence cautioni

The sequence AAB63091.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10477 mRNA. Translation: CAA71502.1.
U93215 Genomic DNA. Translation: AAB63091.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08389.1.
AY128354 mRNA. Translation: AAM91557.1.
BT020607 mRNA. Translation: AAW80880.1.
PIRiE84708.
RefSeqiNP_180603.2. NM_128597.2.
UniGeneiAt.10032.

Genome annotation databases

EnsemblPlantsiAT2G30440.1; AT2G30440.1; AT2G30440.
GeneIDi817595.
GrameneiAT2G30440.1; AT2G30440.1; AT2G30440.
KEGGiath:AT2G30440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10477 mRNA. Translation: CAA71502.1.
U93215 Genomic DNA. Translation: AAB63091.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08389.1.
AY128354 mRNA. Translation: AAM91557.1.
BT020607 mRNA. Translation: AAW80880.1.
PIRiE84708.
RefSeqiNP_180603.2. NM_128597.2.
UniGeneiAt.10032.

3D structure databases

ProteinModelPortaliO04348.
SMRiO04348. Positions 180-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G30440.1.

Protein family/group databases

MEROPSiS26.008.

Proteomic databases

PaxDbiO04348.
PRIDEiO04348.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30440.1; AT2G30440.1; AT2G30440.
GeneIDi817595.
GrameneiAT2G30440.1; AT2G30440.1; AT2G30440.
KEGGiath:AT2G30440.

Organism-specific databases

TAIRiAT2G30440.

Phylogenomic databases

eggNOGiKOG0171. Eukaryota.
COG0681. LUCA.
HOGENOMiHOG000243179.
InParanoidiO04348.
KOiK03100.
OMAiDTIYHDQ.
PhylomeDBiO04348.

Enzyme and pathway databases

BioCyciARA:AT2G30440-MONOMER.

Miscellaneous databases

PROiO04348.

Gene expression databases

GenevisibleiO04348. AT.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR015927. Peptidase_S24_S26A/B/C.
IPR019533. Peptidase_S26.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF10502. Peptidase_S26. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a cDNA encoding the thylakoidal processing peptidase from Arabidopsis thaliana. Implications for the origin and catalytic mechanism of the enzyme."
    Chaal B.K., Mould R.M., Barbrook A.C., Gray J.C., Howe C.J.
    J. Biol. Chem. 273:689-692(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiTPP1_ARATH
AccessioniPrimary (citable) accession number: O04348
Secondary accession number(s): Q7DM64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.