Reviewed,
UniProtKB/Swiss-Prot O04348 (TPP1_ARATH)
Last modified
February 9, 2010.
Version 72.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Thylakoidal processing peptidase 1, chloroplastic EC=3.4.21.89 Alternative name(s): Signal peptidase I-1 | ||||||
| Gene names |
| ||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 340 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Cleaves the thylakoid-transfer domain from a chloroplast protein. Ref.1 |
| Catalytic activity | Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins. |
| Subcellular location | Plastid › chloroplast thylakoid membrane; Single-pass membrane protein. Note: located in the non-appressed lamellae of the thylakoid network By similarity. Ref.1 |
| Sequence similarities | Belongs to the peptidase S26 family. |
| Sequence caution | The sequence AAB63091.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Chloroplast Membrane Plastid Thylakoid |
| Domain | Transit peptide Transmembrane |
| Molecular function | Hydrolase Protease |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: InterPro signal peptide processing Ref.1Inferred from direct assay. Source: TAIR |
| Cellular component | chloroplast thylakoid Ref.1 Inferred from direct assay. Source: TAIR integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | endopeptidase activity Ref.1 Inferred from direct assay. Source: TAIR serine-type peptidase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 52 | 52 | Chloroplast Potential | ||||||
| Transit peptide | 53 – ? | Thylakoid Potential | |||||||
| Chain | ? – 340 | Thylakoidal processing peptidase 1, chloroplastic | PRO_0000310734 | ||||||
Regions | |||||||||
| Transmembrane | 155 – 175 | 21 | Potential | ||||||
| Topological domain | 176 – 340 | 165 | Lumenal, thylakoid Potential | ||||||
| Compositional bias | 131 – 135 | 5 | Poly-Asp | ||||||
Sites | |||||||||
| Active site | 184 | 1 | By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of a cDNA encoding the thylakoidal processing peptidase from Arabidopsis thaliana. Implications for the origin and catalytic mechanism of the enzyme." Chaal B.K., Mould R.M., Barbrook A.C., Gray J.C., Howe C.J. J. Biol. Chem. 273:689-692(1998) [PubMed: 9422718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C.Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y10477 mRNA. Translation: CAA71502.1. U93215 Genomic DNA. Translation: AAB63091.1. Sequence problems. AY128354 mRNA. Translation: AAM91557.1. BT020607 mRNA. Translation: AAW80880.1. |
| IPI | IPI00520820. |
| PIR | E84708. |
| RefSeq | NP_180603.2. |
| UniGene | At.10032 Rra.24397 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1KN9 based on UniProtKB P00803. |
| SMR | O04348. Positions 171-321. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S26.008. |
Genome annotation databases | |
| GeneID | 817595. |
| GenomeReviews | Gene locus AT2G30440 in contig CT485783_GR. |
| KEGG | ath:AT2G30440. |
| NMPDR | fig|3702.1.peg.10095. |
Organism-specific databases | |
| GeneFarm | 2455. 205. |
| TAIR | At2g30440. |
Phylogenomic databases | |
| eggNOG | KOG0171. |
| HOGENOM | HBG595105. |
| InParanoid | O04348. |
| PhylomeDB | O04348. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.89. 302. |
Gene expression databases | |
| Genevestigator | O04348. |
Family and domain databases | |
| InterPro | IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view] |
| Gene3D | G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. |
| PRINTS | PR00727. LEADERPTASE. |
| TIGRFAMs | TIGR02227. sigpep_I_bact. 1 hit. |
| PROSITE | PS00501. SPASE_I_1. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | TPP1_ARATH | ||||||||
| Accession | Primary (citable) accession number: O04348 Secondary accession number(s): Q7DM64 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


