Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prohibitin-3, mitochondrial

Gene

PHB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins (By similarity). Necessary for mitochondrial and cell metabolism and biogenesis. Required to regulate the ethylene-mediated signaling; involved in growth maintenance in the presence of ethylene. Functions in nitric oxide (NO)-mediated responses and in hydrogen peroxide-induced NO accumulation.By similarity3 Publications

GO - Biological processi

  • cell division Source: TAIR
  • lateral root development Source: TAIR
  • mitochondrion organization Source: TAIR
  • negative regulation of cell division Source: TAIR
  • response to auxin Source: TAIR
  • response to ethylene Source: UniProtKB
  • response to nitric oxide Source: TAIR
  • response to salt stress Source: TAIR

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin-3, mitochondrial
Short name:
Atphb3
Alternative name(s):
Protein ENHANCED ETHYLENE RESPONSE 3
Gene namesi
Name:PHB3
Synonyms:EER3
Ordered Locus Names:At5g40770
ORF Names:K1B16.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40770
TAIRilocus:2154810 AT5G40770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 6Mitochondrial matrixSequence analysis5
Transmembranei7 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST22
Topological domaini29 – 277Mitochondrial intermembraneSequence analysisAdd BLAST249

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Extreme constitutive ethylene response in air associated with a partial loss of ethylene-inducible gene expression and an increased ethylene production. Mitochondrial swelling, decreased meristematic cell production, increased cell division time and reduced cell expansion rates, leading to severe growth retardation. Reduced sensitivity to salt stress and defective in H2O2-induced nitric oxide (NO) accumulation, light-induced NO in cotyledons, abscisic acid (ABA)-induced NO accumulation and stomatal closure, and in auxin-induced lateral root formation.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37G → D in phb3-3; reduced sensitivity to salt stress and defective in H(2)O(2)-induced nitric oxide (NO) accumulation, light-induced NO in cotyledons, abscisic acid (ABA)-induced NO accumulation and stomatal closure, and in auxin-induced lateral root formation. 1 Publication1
Mutagenesisi165D → N in eer3-1; increased sensitivity and profound exaggeration of response to ethylene, as well as increased ethylene production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004205982 – 277Prohibitin-3, mitochondrialAdd BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO04331
PRIDEiO04331
ProMEXiO04331

PTM databases

iPTMnetiO04331

Expressioni

Tissue specificityi

Mostly expressed in proliferative tissues, including vasculature, shoot and root apical tissues. Expressed in roots, stems, leaves and flowers (at protein level).

Gene expression databases

ExpressionAtlasiO04331 baseline and differential
GenevisibleiO04331 AT

Interactioni

Subunit structurei

Component of a prohibitin multimeric complex in mitochondrial membranes.3 Publications

Protein-protein interaction databases

BioGridi19328, 1 interactor
IntActiO04331, 3 interactors
STRINGi3702.AT5G40770.1

Structurei

3D structure databases

ProteinModelPortaliO04331
SMRiO04331
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prohibitin family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3083 Eukaryota
COG0330 LUCA
HOGENOMiHOG000205692
InParanoidiO04331
KOiK17080
OMAiVINEGTH
OrthoDBiEOG09360JD1
PhylomeDBiO04331

Family and domain databases

CDDicd03401 SPFH_prohibitin, 1 hit
InterProiView protein in InterPro
IPR001107 Band_7
IPR036013 Band_7/SPFH_dom_sf
IPR000163 Prohibitin
PANTHERiPTHR23222 PTHR23222, 1 hit
PfamiView protein in Pfam
PF01145 Band_7, 1 hit
PRINTSiPR00679 PROHIBITIN
SMARTiView protein in SMART
SM00244 PHB, 1 hit
SUPFAMiSSF117892 SSF117892, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSQQAAVSF LSNLAKAAFG LGTAATVLNT SLFTVDGGER AVIFDRFRGV
60 70 80 90 100
MDQTVGEGTH FLIPILQRPH IFDIRTKPHT FSSISGTKDL QMVNLTLRVL
110 120 130 140 150
SRPEVSRLPY IFQTLGLEYD EKVLPSIGNE VLKAVVAQFN ADQLLTERPH
160 170 180 190 200
VSALVRESLI TRAKDFNIVL DDVAITHLSY GVEFSRAVEQ KQVAQQEAER
210 220 230 240 250
SKFVVMKADQ ERRAAVIRAE GESEAAQLIS DATAKAGMGL IELRRIEASR
260 270
EIASTLARSP NVAYLPGGQS MLFALNR
Length:277
Mass (Da):30,400
Last modified:July 1, 1997 - v1
Checksum:i1D57190F11370693
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69155 mRNA Translation: AAC49691.1
U66593 mRNA Translation: AAD00157.1
AB015470 Genomic DNA Translation: BAB08838.1
CP002688 Genomic DNA Translation: AED94592.1
AY054499 mRNA Translation: AAK96690.1
AY114631 mRNA Translation: AAM47950.1
AY087641 mRNA Translation: AAM65180.1
RefSeqiNP_198893.1, NM_123442.5
UniGeneiAt.6861

Genome annotation databases

EnsemblPlantsiAT5G40770.1; AT5G40770.1; AT5G40770
GeneIDi834077
GrameneiAT5G40770.1; AT5G40770.1; AT5G40770
KEGGiath:AT5G40770

Similar proteinsi

Entry informationi

Entry nameiPHB3_ARATH
AccessioniPrimary (citable) accession number: O04331
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health