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Protein

Prohibitin-3, mitochondrial

Gene

PHB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins (By similarity). Necessary for mitochondrial and cell metabolism and biogenesis. Required to regulate the ethylene-mediated signaling; involved in growth maintenance in the presence of ethylene. Functions in nitric oxide (NO)-mediated responses and in hydrogen peroxide-induced NO accumulation.By similarity3 Publications

GO - Biological processi

  • cell division Source: TAIR
  • cell growth Source: TAIR
  • lateral root development Source: TAIR
  • mitochondrion organization Source: TAIR
  • response to auxin Source: TAIR
  • response to ethylene Source: UniProtKB
  • response to nitric oxide Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Prohibitin-3, mitochondrial
Short name:
Atphb3
Alternative name(s):
Protein ENHANCED ETHYLENE RESPONSE 3
Gene namesi
Name:PHB3
Synonyms:EER3
Ordered Locus Names:At5g40770
ORF Names:K1B16.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G40770.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 65Mitochondrial matrixSequence analysis
Transmembranei7 – 2822Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 277249Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • mitochondrial respiratory chain complex I Source: TAIR
  • mitochondrion Source: TAIR
  • nucleolus Source: TAIR
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Extreme constitutive ethylene response in air associated with a partial loss of ethylene-inducible gene expression and an increased ethylene production. Mitochondrial swelling, decreased meristematic cell production, increased cell division time and reduced cell expansion rates, leading to severe growth retardation. Reduced sensitivity to salt stress and defective in H2O(2)-induced nitric oxide (NO) accumulation, light-induced NO in cotyledons, abscisic acid (ABA)-induced NO accumulation and stomatal closure, and in auxin-induced lateral root formation.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi37 – 371G → D in phb3-3; reduced sensitivity to salt stress and defective in H(2)O(2)-induced nitric oxide (NO) accumulation, light-induced NO in cotyledons, abscisic acid (ABA)-induced NO accumulation and stomatal closure, and in auxin-induced lateral root formation. 1 Publication
Mutagenesisi165 – 1651D → N in eer3-1; increased sensitivity and profound exaggeration of response to ethylene, as well as increased ethylene production. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 277276Prohibitin-3, mitochondrialPRO_0000420598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO04331.
PRIDEiO04331.
ProMEXiO04331.

Expressioni

Tissue specificityi

Mostly expressed in proliferative tissues, including vasculature, shoot and root apical tissues. Expressed in roots, stems, leaves, flowers and pollen (at protein level).4 Publications

Gene expression databases

GenevisibleiO04331. AT.

Interactioni

Subunit structurei

Component of a prohibitin multimeric complex in mitochondrial membranes.3 Publications

Protein-protein interaction databases

BioGridi19328. 1 interaction.
IntActiO04331. 3 interactions.
STRINGi3702.AT5G40770.1.

Structurei

3D structure databases

ProteinModelPortaliO04331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prohibitin family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3083. Eukaryota.
COG0330. LUCA.
HOGENOMiHOG000205692.
InParanoidiO04331.
KOiK17080.
OMAiIMIRRIE.
PhylomeDBiO04331.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSQQAAVSF LSNLAKAAFG LGTAATVLNT SLFTVDGGER AVIFDRFRGV
60 70 80 90 100
MDQTVGEGTH FLIPILQRPH IFDIRTKPHT FSSISGTKDL QMVNLTLRVL
110 120 130 140 150
SRPEVSRLPY IFQTLGLEYD EKVLPSIGNE VLKAVVAQFN ADQLLTERPH
160 170 180 190 200
VSALVRESLI TRAKDFNIVL DDVAITHLSY GVEFSRAVEQ KQVAQQEAER
210 220 230 240 250
SKFVVMKADQ ERRAAVIRAE GESEAAQLIS DATAKAGMGL IELRRIEASR
260 270
EIASTLARSP NVAYLPGGQS MLFALNR
Length:277
Mass (Da):30,400
Last modified:July 1, 1997 - v1
Checksum:i1D57190F11370693
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69155 mRNA. Translation: AAC49691.1.
U66593 mRNA. Translation: AAD00157.1.
AB015470 Genomic DNA. Translation: BAB08838.1.
CP002688 Genomic DNA. Translation: AED94592.1.
AY054499 mRNA. Translation: AAK96690.1.
AY114631 mRNA. Translation: AAM47950.1.
AY087641 mRNA. Translation: AAM65180.1.
RefSeqiNP_198893.1. NM_123442.4.
UniGeneiAt.6861.

Genome annotation databases

EnsemblPlantsiAT5G40770.1; AT5G40770.1; AT5G40770.
GeneIDi834077.
GrameneiAT5G40770.1; AT5G40770.1; AT5G40770.
KEGGiath:AT5G40770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69155 mRNA. Translation: AAC49691.1.
U66593 mRNA. Translation: AAD00157.1.
AB015470 Genomic DNA. Translation: BAB08838.1.
CP002688 Genomic DNA. Translation: AED94592.1.
AY054499 mRNA. Translation: AAK96690.1.
AY114631 mRNA. Translation: AAM47950.1.
AY087641 mRNA. Translation: AAM65180.1.
RefSeqiNP_198893.1. NM_123442.4.
UniGeneiAt.6861.

3D structure databases

ProteinModelPortaliO04331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19328. 1 interaction.
IntActiO04331. 3 interactions.
STRINGi3702.AT5G40770.1.

Proteomic databases

PaxDbiO04331.
PRIDEiO04331.
ProMEXiO04331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G40770.1; AT5G40770.1; AT5G40770.
GeneIDi834077.
GrameneiAT5G40770.1; AT5G40770.1; AT5G40770.
KEGGiath:AT5G40770.

Organism-specific databases

TAIRiAT5G40770.

Phylogenomic databases

eggNOGiKOG3083. Eukaryota.
COG0330. LUCA.
HOGENOMiHOG000205692.
InParanoidiO04331.
KOiK17080.
OMAiIMIRRIE.
PhylomeDBiO04331.

Miscellaneous databases

PROiO04331.

Gene expression databases

GenevisibleiO04331. AT.

Family and domain databases

InterProiIPR001107. Band_7.
IPR000163. Prohibitin.
[Graphical view]
PANTHERiPTHR23222. PTHR23222. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00679. PROHIBITIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the plant homologue of prohibitin, a gene associated with antiproliferative activity in mammalian cells."
    Snedden W.A., Fromm H.
    Plant Mol. Biol. 33:753-756(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  2. "Arabidopsis genes encoding prohibitin: importance for early development."
    Sun L., Goodman H.M.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian and fungal components coupled with plant-specific subunits."
    Heazlewood J.L., Howell K.A., Millar A.H.
    Biochim. Biophys. Acta 1604:159-169(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  10. "Proteome mapping of mature pollen of Arabidopsis thaliana."
    Holmes-Davis R., Tanaka C.K., Vensel W.H., Hurkman W.J., McCormick S.
    Proteomics 5:4864-4884(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY [LARGE SCALE ANALYSIS].
  11. "Mutational loss of the prohibitin AtPHB3 results in an extreme constitutive ethylene response phenotype coupled with partial loss of ethylene-inducible gene expression in Arabidopsis seedlings."
    Christians M.J., Larsen P.B.
    J. Exp. Bot. 58:2237-2248(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-165, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  12. "Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development."
    Van Aken O., Pecenkova T., van de Cotte B., De Rycke R., Eeckhout D., Fromm H., De Jaeger G., Witters E., Beemster G.T.S., Inze D., Van Breusegem F.
    Plant J. 52:850-864(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: cv. Columbia.
  13. "Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis."
    Meyer E.H., Taylor N.L., Millar A.H.
    J. Proteome Res. 7:786-794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, SUBUNIT.
  14. "The Arabidopsis prohibitin gene PHB3 functions in nitric oxide-mediated responses and in hydrogen peroxide-induced nitric oxide accumulation."
    Wang Y., Ries A., Wu K., Yang A., Crawford N.M.
    Plant Cell 22:249-259(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-37.
  15. "Defining the protein complex proteome of plant mitochondria."
    Klodmann J., Senkler M., Rode C., Braun H.-P.
    Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  16. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPHB3_ARATH
AccessioniPrimary (citable) accession number: O04331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: July 1, 1997
Last modified: February 17, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.