ID   NUP35_ARATH             Reviewed;         329 AA.
AC   O04326; Q8VYL9;
DT   26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   24-JAN-2024, entry version 143.
DE   RecName: Full=Nuclear pore complex protein NUP35 {ECO:0000303|PubMed:21189294};
DE   AltName: Full=Nucleoporin 35;
GN   Name=NUP35 {ECO:0000303|PubMed:21189294};
GN   OrderedLocusNames=At3g16310 {ECO:0000312|Araport:AT3G16310};
GN   ORFNames=T02O04.20 {ECO:0000312|EMBL:AAB63646.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=cv. Columbia;
RX   PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA   Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA   Rathjen J.P., Peck S.C.;
RT   "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT   thaliana.";
RL   J. Proteomics 72:439-451(2009).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
RN   [8]
RP   IDENTIFICATION IN THE NUCLEAR PORE COMPLEX, AND NOMENCLATURE.
RX   PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA   Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT   "Identification and characterization of nuclear pore complex components in
RT   Arabidopsis thaliana.";
RL   Plant Cell 22:4084-4097(2010).
CC   -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC       fold symmetrical structure comprising a central transport channel and
CC       two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC       are attached. The cytoplasmic filaments have loose ends, while the
CC       nuclear filaments are joined in a distal ring, forming a nuclear
CC       basket. NPCs are highly dynamic in configuration and composition, and
CC       can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC       subcomplex and the NUP93 subcomplex, containing approximately 30
CC       different nucleoporin proteins. {ECO:0000305|PubMed:21189294}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC       {ECO:0000305|PubMed:21189294}.
CC   -!- SIMILARITY: Belongs to the Nup35 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB023046; BAB01270.1; -; Genomic_DNA.
DR   EMBL; AC001645; AAB63646.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75796.1; -; Genomic_DNA.
DR   EMBL; AY086937; AAM64501.1; -; mRNA.
DR   EMBL; BT002339; AAN86172.1; -; mRNA.
DR   EMBL; AY070444; AAL49847.1; -; mRNA.
DR   RefSeq; NP_566542.1; NM_112502.5.
DR   AlphaFoldDB; O04326; -.
DR   SMR; O04326; -.
DR   BioGRID; 6212; 63.
DR   IntAct; O04326; 72.
DR   STRING; 3702.O04326; -.
DR   iPTMnet; O04326; -.
DR   PaxDb; 3702-AT3G16310-1; -.
DR   ProteomicsDB; 248640; -.
DR   EnsemblPlants; AT3G16310.1; AT3G16310.1; AT3G16310.
DR   GeneID; 820878; -.
DR   Gramene; AT3G16310.1; AT3G16310.1; AT3G16310.
DR   KEGG; ath:AT3G16310; -.
DR   Araport; AT3G16310; -.
DR   TAIR; AT3G16310; -.
DR   eggNOG; KOG4285; Eukaryota.
DR   HOGENOM; CLU_042110_0_0_1; -.
DR   InParanoid; O04326; -.
DR   OMA; AGMMING; -.
DR   OrthoDB; 1068173at2759; -.
DR   PhylomeDB; O04326; -.
DR   PRO; PR:O04326; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; O04326; baseline and differential.
DR   GO; GO:0031965; C:nuclear membrane; IEA:InterPro.
DR   GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0017056; F:structural constituent of nuclear pore; IEA:InterPro.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   CDD; cd12441; RRM_Nup53_like; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR017389; Nucleoporin_NUP53.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR007846; RRM_NUP35_dom.
DR   PANTHER; PTHR21527; NUCLEOPORIN NUP35; 1.
DR   PANTHER; PTHR21527:SF6; NUCLEOPORIN NUP35; 1.
DR   Pfam; PF05172; Nup35_RRM; 1.
DR   PIRSF; PIRSF038119; Nucleoporin_NUP53; 1.
DR   SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
DR   PROSITE; PS51472; RRM_NUP35; 1.
DR   Genevisible; O04326; AT.
PE   1: Evidence at protein level;
KW   mRNA transport; Nuclear pore complex; Nucleus; Protein transport;
KW   Reference proteome; Translocation; Transport.
FT   CHAIN           1..329
FT                   /note="Nuclear pore complex protein NUP35"
FT                   /id="PRO_0000431082"
FT   DOMAIN          183..264
FT                   /note="RRM Nup35-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00804"
FT   REGION          48..105
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          123..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          271..315
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        85..99
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        123..138
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        271..314
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        329
FT                   /note="V -> A (in Ref. 4; AAL49847)"
SQ   SEQUENCE   329 AA;  35430 MW;  55BB007762A65D05 CRC64;
     MSAAAHRTPK SGRQSLLFQD LASPVSARRG KFSSPGQAAA VSALWRENFG GSDLPPPPMY
     TLDDRSDFSP ESGIADYSAS PDAKSDRRTP FQSSGKNIVT PGKGKLEASP SFSLLNAQQS
     QQVSGSPSWW SQSKAGSSTE QDDKGKGSPV EGVVQPGALV TLPPPREVAR PEVQRQIIPT
     GNLDEEEWVT VYGFSPGDTN LVLREFEKCG MVLKHVPGPR NANWMHILYQ NRSDAHKALN
     KAGMMINGVV IVGVKPVDPI QKQALNERLN NQGFMPLPPP SSTRNTARPL SRPQYLQNGS
     AFSPQPSGGA MASPSKSMVS KFFDLMFGV
//