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Protein

PYK10-binding protein 1

Gene

PBP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor-type lectin that may regulate the correct polymerization of BGLU23/PYK10 upon tissue damage. Activates BGLU21, BGLU22 and BGLU23.3 Publications

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • enzyme regulator activity Source: TAIR

GO - Biological processi

  • protein folding Source: TAIR
  • regulation of hydrolase activity Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
PYK10-binding protein 1
Alternative name(s):
Jacalin-related lectin 30
Jasmonic acid-induced protein
Gene namesi
Name:PBP1
Synonyms:JAL30, JIP, PBPI
Ordered Locus Names:At3g16420
ORF Names:MDC8.5, T02O04.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G16420.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduced tissue damage-mediated BGLU23/PYK10 activation. Larger PYK10 complexes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 298297PYK10-binding protein 1PRO_0000422166Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei20 – 201PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO04314.
PRIDEiO04314.

PTM databases

iPTMnetiO04314.

Expressioni

Tissue specificityi

Expressed exclusively in roots.1 Publication

Inductioni

Induced by NAI1 (at protein level).1 Publication

Gene expression databases

GenevisibleiO04314. AT.

Interactioni

Subunit structurei

Component of the PYK10 complex, at least composed of PYK10/BGLU23, BGLU21, BGLU22, JAL22, JAL23, PBP1/JAL30, PBP2/JAL31, JAL32, JAL33, JAL34, JAL35, GLL22 and GLL23.1 Publication

Protein-protein interaction databases

BioGridi6224. 2 interactions.
STRINGi3702.AT3G16420.1.

Structurei

3D structure databases

ProteinModelPortaliO04314.
SMRiO04314. Positions 2-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 142141Jacalin-type lectin 1PROSITE-ProRule annotationAdd
BLAST
Domaini152 – 295144Jacalin-type lectin 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the jacalin lectin family.PROSITE-ProRule annotationCurated
Contains 2 jacalin-type lectin domains.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410JWUK. Eukaryota.
ENOG41104KM. LUCA.
HOGENOMiHOG000015149.
InParanoidiO04314.
OMAiVEGWYSP.
PhylomeDBiO04314.

Family and domain databases

Gene3Di2.100.10.30. 2 hits.
InterProiIPR001229. Jacalin-like_lectin_dom.
[Graphical view]
PfamiPF01419. Jacalin. 2 hits.
[Graphical view]
SMARTiSM00915. Jacalin. 2 hits.
[Graphical view]
SUPFAMiSSF51101. SSF51101. 2 hits.
PROSITEiPS51752. JACALIN_LECTIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O04314-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQKVEAQGG KGANLWDDGS THDAVTKIQL AAGIDGIQYV QFDYVKNGQP
60 70 80 90 100
EQAPLRGTKG RVLPADPFVI NHPDEHLVSV EGWYSPEGII QGIKFISNKK
110 120 130 140 150
TSDVIGSDEG THFTLQVKDK KIIGFHGSAG GNLNSLGAYF APLTTTTPLT
160 170 180 190 200
PAKQLTAFGS DDGTVWDDGA YVGVKKVYVG QAQDGISAVK FVYDKSPEEV
210 220 230 240 250
TGEEHGKSTL LGFEEFVLDY PSEYITAVDG TYDKIFGSDG SVITMLRFKT
260 270 280 290
NKQTSPPFGL EAGTVFELKE EGHKIVGFHG RADVLLHKIG VHVRPLSN
Length:298
Mass (Da):32,158
Last modified:July 1, 1997 - v1
Checksum:i329819548BC98627
GO
Isoform 2 (identifier: O04314-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     87-108: Missing.

Note: No experimental confirmation available.
Show »
Length:276
Mass (Da):29,826
Checksum:iDC650043F0A7E381
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931I → V in AAM65935 (Ref. 6) Curated
Sequence conflicti165 – 1651V → A in AAM65935 (Ref. 6) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei87 – 10822Missing in isoform 2. 1 PublicationVSP_046471Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000373 Genomic DNA. Translation: BAB01141.1.
AC001645 Genomic DNA. Translation: AAB63635.1.
CP002686 Genomic DNA. Translation: AEE75810.1.
CP002686 Genomic DNA. Translation: AEE75811.1.
CP002686 Genomic DNA. Translation: AEE75812.1.
AF370488 mRNA. Translation: AAK43865.1.
AY064653 mRNA. Translation: AAL47364.1.
AY065189 mRNA. Translation: AAL38365.1.
AY128360 mRNA. Translation: AAM91563.1.
BT000021 mRNA. Translation: AAN15340.1.
BT002196 mRNA. Translation: AAN72207.1.
AK319056 mRNA. Translation: BAH57171.1.
AY088397 mRNA. Translation: AAM65935.1.
RefSeqiNP_001030710.1. NM_001035633.1. [O04314-1]
NP_188263.1. NM_112513.2. [O04314-1]
NP_850594.1. NM_180263.2. [O04314-1]
UniGeneiAt.22943.
At.43522.
At.67080.
At.71528.

Genome annotation databases

EnsemblPlantsiAT3G16420.1; AT3G16420.1; AT3G16420. [O04314-1]
AT3G16420.2; AT3G16420.2; AT3G16420. [O04314-1]
AT3G16420.3; AT3G16420.3; AT3G16420. [O04314-1]
GeneIDi820890.
KEGGiath:AT3G16420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000373 Genomic DNA. Translation: BAB01141.1.
AC001645 Genomic DNA. Translation: AAB63635.1.
CP002686 Genomic DNA. Translation: AEE75810.1.
CP002686 Genomic DNA. Translation: AEE75811.1.
CP002686 Genomic DNA. Translation: AEE75812.1.
AF370488 mRNA. Translation: AAK43865.1.
AY064653 mRNA. Translation: AAL47364.1.
AY065189 mRNA. Translation: AAL38365.1.
AY128360 mRNA. Translation: AAM91563.1.
BT000021 mRNA. Translation: AAN15340.1.
BT002196 mRNA. Translation: AAN72207.1.
AK319056 mRNA. Translation: BAH57171.1.
AY088397 mRNA. Translation: AAM65935.1.
RefSeqiNP_001030710.1. NM_001035633.1. [O04314-1]
NP_188263.1. NM_112513.2. [O04314-1]
NP_850594.1. NM_180263.2. [O04314-1]
UniGeneiAt.22943.
At.43522.
At.67080.
At.71528.

3D structure databases

ProteinModelPortaliO04314.
SMRiO04314. Positions 2-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6224. 2 interactions.
STRINGi3702.AT3G16420.1.

PTM databases

iPTMnetiO04314.

Proteomic databases

PaxDbiO04314.
PRIDEiO04314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G16420.1; AT3G16420.1; AT3G16420. [O04314-1]
AT3G16420.2; AT3G16420.2; AT3G16420. [O04314-1]
AT3G16420.3; AT3G16420.3; AT3G16420. [O04314-1]
GeneIDi820890.
KEGGiath:AT3G16420.

Organism-specific databases

TAIRiAT3G16420.

Phylogenomic databases

eggNOGiENOG410JWUK. Eukaryota.
ENOG41104KM. LUCA.
HOGENOMiHOG000015149.
InParanoidiO04314.
OMAiVEGWYSP.
PhylomeDBiO04314.

Miscellaneous databases

PROiO04314.

Gene expression databases

GenevisibleiO04314. AT.

Family and domain databases

Gene3Di2.100.10.30. 2 hits.
InterProiIPR001229. Jacalin-like_lectin_dom.
[Graphical view]
PfamiPF01419. Jacalin. 2 hits.
[Graphical view]
SMARTiSM00915. Jacalin. 2 hits.
[Graphical view]
SUPFAMiSSF51101. SSF51101. 2 hits.
PROSITEiPS51752. JACALIN_LECTIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. "NAI1 gene encodes a basic-helix-loop-helix-type putative transcription factor that regulates the formation of an endoplasmic reticulum-derived structure, the ER body."
    Matsushima R., Fukao Y., Nishimura M., Hara-Nishimura I.
    Plant Cell 16:1536-1549(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION BY NAI1.
  8. "Activation of an ER-body-localized beta-glucosidase via a cytosolic binding partner in damaged tissues of Arabidopsis thaliana."
    Nagano A.J., Matsushima R., Hara-Nishimura I.
    Plant Cell Physiol. 46:1140-1148(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INTERACTION WITH BGLU23/PYK10.
    Strain: cv. Columbia.
  9. "Antagonistic jacalin-related lectins regulate the size of ER body-type beta-glucosidase complexes in Arabidopsis thaliana."
    Nagano A.J., Fukao Y., Fujiwara M., Nishimura M., Hara-Nishimura I.
    Plant Cell Physiol. 49:969-980(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE PYK10 COMPLEX, GENE FAMILY, NOMENCLATURE.
  10. "Scopolin-hydrolyzing beta-glucosidases in roots of Arabidopsis."
    Ahn Y.O., Shimizu B., Sakata K., Gantulga D., Zhou C., Zhou Z., Bevan D.R., Esen A.
    Plant Cell Physiol. 51:132-143(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "Identification of phosphoproteins in Arabidopsis thaliana leaves using polyethylene glycol fractionation, immobilized metal-ion affinity chromatography, two-dimensional gel electrophoresis and mass spectrometry."
    Aryal U.K., Krochko J.E., Ross A.R.
    J. Proteome Res. 11:425-437(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "ERMO3/MVP1/GOLD36 is involved in a cell type-specific mechanism for maintaining ER morphology in Arabidopsis thaliana."
    Nakano R.T., Matsushima R., Nagano A.J., Fukao Y., Fujiwara M., Kondo M., Nishimura M., Hara-Nishimura I.
    PLoS ONE 7:E49103-E49103(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BGLU23/PYK10.

Entry informationi

Entry nameiJAL30_ARATH
AccessioniPrimary (citable) accession number: O04314
Secondary accession number(s): C0Z392, Q8L9J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: July 1, 1997
Last modified: January 20, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

PubMed:18467340 shows that PBP1 inhibits polymerization of PYK10 complex, while in PubMed:19965874, PBP1 activates purified PYK10.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.