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Protein

PYK10-binding protein 1

Gene

PBP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor-type lectin that may regulate the correct polymerization of BGLU23/PYK10 upon tissue damage. Activates BGLU21, BGLU22 and BGLU23.3 Publications

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • enzyme regulator activity Source: TAIR

GO - Biological processi

  • protein folding Source: TAIR
  • regulation of hydrolase activity Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
PYK10-binding protein 1
Alternative name(s):
Jacalin-related lectin 30
Jasmonic acid-induced protein
Gene namesi
Name:PBP1
Synonyms:JAL30, JIP, PBPI
Ordered Locus Names:At3g16420
ORF Names:MDC8.5, T02O04.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G16420.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduced tissue damage-mediated BGLU23/PYK10 activation. Larger PYK10 complexes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004221662 – 298PYK10-binding protein 1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei20PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO04314.
PRIDEiO04314.

PTM databases

iPTMnetiO04314.

Expressioni

Tissue specificityi

Expressed exclusively in roots.1 Publication

Inductioni

Induced by NAI1 (at protein level).1 Publication

Gene expression databases

GenevisibleiO04314. AT.

Interactioni

Subunit structurei

Component of the PYK10 complex, at least composed of PYK10/BGLU23, BGLU21, BGLU22, JAL22, JAL23, PBP1/JAL30, PBP2/JAL31, JAL32, JAL33, JAL34, JAL35, GLL22 and GLL23.1 Publication

Protein-protein interaction databases

BioGridi6224. 2 interactors.
STRINGi3702.AT3G16420.1.

Structurei

3D structure databases

ProteinModelPortaliO04314.
SMRiO04314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 142Jacalin-type lectin 1PROSITE-ProRule annotationAdd BLAST141
Domaini152 – 295Jacalin-type lectin 2PROSITE-ProRule annotationAdd BLAST144

Sequence similaritiesi

Belongs to the jacalin lectin family.PROSITE-ProRule annotationCurated
Contains 2 jacalin-type lectin domains.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410JWUK. Eukaryota.
ENOG41104KM. LUCA.
HOGENOMiHOG000015149.
InParanoidiO04314.
OMAiVEGWYSP.
OrthoDBiEOG093605NA.
PhylomeDBiO04314.

Family and domain databases

CDDicd09612. Jacalin. 2 hits.
Gene3Di2.100.10.30. 2 hits.
InterProiIPR001229. Jacalin-like_lectin_dom.
IPR033734. Jacalin-like_lectin_dom_plant.
[Graphical view]
PfamiPF01419. Jacalin. 2 hits.
[Graphical view]
SMARTiSM00915. Jacalin. 2 hits.
[Graphical view]
SUPFAMiSSF51101. SSF51101. 2 hits.
PROSITEiPS51752. JACALIN_LECTIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O04314-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQKVEAQGG KGANLWDDGS THDAVTKIQL AAGIDGIQYV QFDYVKNGQP
60 70 80 90 100
EQAPLRGTKG RVLPADPFVI NHPDEHLVSV EGWYSPEGII QGIKFISNKK
110 120 130 140 150
TSDVIGSDEG THFTLQVKDK KIIGFHGSAG GNLNSLGAYF APLTTTTPLT
160 170 180 190 200
PAKQLTAFGS DDGTVWDDGA YVGVKKVYVG QAQDGISAVK FVYDKSPEEV
210 220 230 240 250
TGEEHGKSTL LGFEEFVLDY PSEYITAVDG TYDKIFGSDG SVITMLRFKT
260 270 280 290
NKQTSPPFGL EAGTVFELKE EGHKIVGFHG RADVLLHKIG VHVRPLSN
Length:298
Mass (Da):32,158
Last modified:July 1, 1997 - v1
Checksum:i329819548BC98627
GO
Isoform 2 (identifier: O04314-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     87-108: Missing.

Note: No experimental confirmation available.
Show »
Length:276
Mass (Da):29,826
Checksum:iDC650043F0A7E381
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93I → V in AAM65935 (Ref. 6) Curated1
Sequence conflicti165V → A in AAM65935 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04647187 – 108Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000373 Genomic DNA. Translation: BAB01141.1.
AC001645 Genomic DNA. Translation: AAB63635.1.
CP002686 Genomic DNA. Translation: AEE75810.1.
CP002686 Genomic DNA. Translation: AEE75811.1.
CP002686 Genomic DNA. Translation: AEE75812.1.
AF370488 mRNA. Translation: AAK43865.1.
AY064653 mRNA. Translation: AAL47364.1.
AY065189 mRNA. Translation: AAL38365.1.
AY128360 mRNA. Translation: AAM91563.1.
BT000021 mRNA. Translation: AAN15340.1.
BT002196 mRNA. Translation: AAN72207.1.
AK319056 mRNA. Translation: BAH57171.1.
AY088397 mRNA. Translation: AAM65935.1.
RefSeqiNP_001030710.1. NM_001035633.1. [O04314-1]
NP_188263.1. NM_112513.3. [O04314-1]
NP_850594.1. NM_180263.2. [O04314-1]
UniGeneiAt.22943.
At.43522.
At.67080.
At.71528.

Genome annotation databases

EnsemblPlantsiAT3G16420.1; AT3G16420.1; AT3G16420. [O04314-1]
AT3G16420.2; AT3G16420.2; AT3G16420. [O04314-1]
AT3G16420.3; AT3G16420.3; AT3G16420. [O04314-1]
GeneIDi820890.
GrameneiAT3G16420.1; AT3G16420.1; AT3G16420.
AT3G16420.2; AT3G16420.2; AT3G16420.
AT3G16420.3; AT3G16420.3; AT3G16420.
KEGGiath:AT3G16420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000373 Genomic DNA. Translation: BAB01141.1.
AC001645 Genomic DNA. Translation: AAB63635.1.
CP002686 Genomic DNA. Translation: AEE75810.1.
CP002686 Genomic DNA. Translation: AEE75811.1.
CP002686 Genomic DNA. Translation: AEE75812.1.
AF370488 mRNA. Translation: AAK43865.1.
AY064653 mRNA. Translation: AAL47364.1.
AY065189 mRNA. Translation: AAL38365.1.
AY128360 mRNA. Translation: AAM91563.1.
BT000021 mRNA. Translation: AAN15340.1.
BT002196 mRNA. Translation: AAN72207.1.
AK319056 mRNA. Translation: BAH57171.1.
AY088397 mRNA. Translation: AAM65935.1.
RefSeqiNP_001030710.1. NM_001035633.1. [O04314-1]
NP_188263.1. NM_112513.3. [O04314-1]
NP_850594.1. NM_180263.2. [O04314-1]
UniGeneiAt.22943.
At.43522.
At.67080.
At.71528.

3D structure databases

ProteinModelPortaliO04314.
SMRiO04314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6224. 2 interactors.
STRINGi3702.AT3G16420.1.

PTM databases

iPTMnetiO04314.

Proteomic databases

PaxDbiO04314.
PRIDEiO04314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G16420.1; AT3G16420.1; AT3G16420. [O04314-1]
AT3G16420.2; AT3G16420.2; AT3G16420. [O04314-1]
AT3G16420.3; AT3G16420.3; AT3G16420. [O04314-1]
GeneIDi820890.
GrameneiAT3G16420.1; AT3G16420.1; AT3G16420.
AT3G16420.2; AT3G16420.2; AT3G16420.
AT3G16420.3; AT3G16420.3; AT3G16420.
KEGGiath:AT3G16420.

Organism-specific databases

TAIRiAT3G16420.

Phylogenomic databases

eggNOGiENOG410JWUK. Eukaryota.
ENOG41104KM. LUCA.
HOGENOMiHOG000015149.
InParanoidiO04314.
OMAiVEGWYSP.
OrthoDBiEOG093605NA.
PhylomeDBiO04314.

Miscellaneous databases

PROiO04314.

Gene expression databases

GenevisibleiO04314. AT.

Family and domain databases

CDDicd09612. Jacalin. 2 hits.
Gene3Di2.100.10.30. 2 hits.
InterProiIPR001229. Jacalin-like_lectin_dom.
IPR033734. Jacalin-like_lectin_dom_plant.
[Graphical view]
PfamiPF01419. Jacalin. 2 hits.
[Graphical view]
SMARTiSM00915. Jacalin. 2 hits.
[Graphical view]
SUPFAMiSSF51101. SSF51101. 2 hits.
PROSITEiPS51752. JACALIN_LECTIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJAL30_ARATH
AccessioniPrimary (citable) accession number: O04314
Secondary accession number(s): C0Z392, Q8L9J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

PubMed:18467340 shows that PBP1 inhibits polymerization of PYK10 complex, while in PubMed:19965874, PBP1 activates purified PYK10.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.