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Reviewed, UniProtKB/Swiss-Prot O04308 (MPPA2_ARATH)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable mitochondrial-processing peptidase subunit alpha-2
    EC=3.4.24.64
Alternative name(s):
    Alpha-MPP 2
Gene names
Ordered Locus Names: At3g16480
ORF Names: T02O04.2, MDC8.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Cleaves presequences (transit peptides) from mitochondrial protein precursors By similarity.

Catalytic activity

Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

Subunit structure

Heterodimer of alpha and beta subunits By similarity.

Subcellular location

Mitochondrion inner membrane By similarity.

Sequence similarities

Belongs to the peptidase M16 family.

Caution

Does not seem to have a protease activity as it lack the zinc-binding site.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion
Chain? – 499Probable mitochondrial-processing peptidase subunit alpha-2PRO_0000026775

Experimental info

Sequence conflict3041T → K in AAK59675. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
O04308-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 6B8203674FAEC56E

FASTA49954,053
        10         20         30         40         50         60 
MYRTAASRAK ALKGILNHNF RASRYASSSA VATSSSSSSW LSGGYSSSLP SMNIPLAGVS 

        70         80         90        100        110        120 
LPPPLSDHVE PSKLKTTTLP NGLTIATEMS PNPAASIGLY VDCGSIYETP QFRGATHLLE 

       130        140        150        160        170        180 
RMAFKSTLNR SHFRLVREIE AIGGNTSASA SREQMGYTID ALKTYVPEMV EVLIDSVRNP 

       190        200        210        220        230        240 
AFLDWEVNEE LRKVKVEIGE FATNPMGFLL EAVHSAGYSG ALANPLYAPE SAITGLTGEV 

       250        260        270        280        290        300 
LENFVFENYT ASRMVLAASG VDHEELLKVV EPLLSDLPNV PRPAEPKSQY VGGDFRQHTG 

       310        320        330        340        350        360 
GEATHFALAF EVPGWNNEKE AIIATVLQML MGGGGSFSAG GPGKGMHSWL YLRLLNQHQQ 

       370        380        390        400        410        420 
FQSCTAFTSV FNNTGLFGIY GCTSPEFASQ GIELVASEMN AVADGKVNQK HLDRAKAATK 

       430        440        450        460        470        480 
SAILMNLESR MIAAEDIGRQ ILTYGERKPV DQFLKTVDQL TLKDIADFTS KVITKPLTMA 

       490 
TFGDVLNVPS YDSVSKRFR

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References

[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

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Cross-references

Sequence databases

AC001645 Genomic DNA. Translation: AAB63629.1.
AP000373 Genomic DNA. Translation: BAB01147.1.
AY035171 mRNA. Translation: AAK59675.1.
AY142643 mRNA. Translation: AAN13101.1.
IPIIPI00542856.
RefSeqNP_566548.1.
UniGeneAt.23627

3D structure databases

HSSPHSSP built from PDB template 1HR6 based on UniProtKB P11914.
ModBaseSearch...

Protein family/group databases

MEROPSM16.971.

Proteomic databases

PRIDEO04308.

Genome annotation databases

GeneID820896.
GenomeReviewsGene locus AT3G16480 in contig BA000014_GR.
KEGGath:AT3G16480.
NMPDRfig|3702.1.peg.13822.

Organism-specific databases

GeneFarm2294. 202.
TAIRAt3g16480.

Phylogenomic databases

OMAO04308. RMVASED.

Enzyme and pathway databases

BioCycMetaCyc:AT3G16480-MON.
BRENDA3.4.24.64. 302.

Gene expression databases

ArrayExpressO04308.
GermOnlineAT3G16480. Arabidopsis thaliana.

Family and domain databases

InterProIPR011237. Pept_M16_core.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
Gene3DG3DSA:3.30.830.10. Pept_M16_core. 2 hits.
PfamPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
PROSITEPS00143. INSULINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMPPA2_ARATH
AccessionPrimary (citable) accession number: O04308
Secondary accession number(s): Q94C54
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: July 1, 1997
Last modified: June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents