O04300 (UPTG_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,4-glucan-protein synthase [UDP-forming]
EC=2.4.1.-

Alternative name(s):
Reversibly glycosylated polypeptide 1
UDP-glucose:protein transglucosylase
Short name=UPTG

Gene names

Name:	UPTG
Synonyms:	RGP1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible role in the synthesis of cell wall polysaccharides. Ref.1
Catalytic activity	UDP-glucose + protein = UDP + alpha-D-glucosyl-protein. UniProtKB Q9SC19
Enzyme regulation	Inhibited by inhibitor protein (IP) which may be a form of sucrose synthase By similarity.
Subunit structure	Homopentamer or homohexamer By similarity.
Subcellular location	Secreted › cell wall. Cell junction › plasmodesma. Golgi apparatus. Note: Cell wall-associated, with highest concentrations on plasmodesmata. Also located in the Golgi apparatus. Ref.1 Ref.2
Domain	The conserved DXD motif is involved in enzyme activity By similarity.
Post-translational modification	Reversibly glycosylated by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose. Ref.1 Ref.2
Sequence similarities	Belongs to the RGP family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell junction Cell wall Golgi apparatus Secreted
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plasmodesma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycogenin glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Alpha-1,4-glucan-protein synthase [UDP-forming]		PRO_0000221194
Regions
	Motif	103 – 105	3	DXD motif
Sites
	Site	151	1	Required for activity By similarity
	Site	158	1	Required for activity By similarity
Amino acid modifications
	Glycosylation	151	1	N-linked (Glc...) By similarity UniProtKB Q9SAQ2

Sequences

	Sequence		Length	Mass (Da)
	O04300 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 006F92870246DF2D	FASTA	364	41,573
	10 20 30 40 50 60 MASLPKPTPL LKDELDIVIP TIRNLDFLEM WRPFFEQYHL IIVQDGDPSK VIKVPEGFDY 70 80 90 100 110 120 ELYNRNDINR ILGPKASCIS FKDSACRCFG YMVSKKKYIY TIDDDCFVAK DPTGHEINAL 130 140 150 160 170 180 EQHIKNLLSP STPFFFNTLY DPYREGTDFV RGYPFSLREG VPTAVSHGLW LNIPDYDAPT 190 200 210 220 230 240 QLVKPHERNT RFVDAVLTIP KGSLFPMCGM NLAFNRELIG PAMYFGLMGD GQPIGRYDDM 250 260 270 280 290 300 WAGWCIKVIC DHLGYGVKTG LPYIWHSKAS NPFVNLKKEY KGIFWQEEII PFFQAATLSK 310 320 330 340 350 360 DCTSVQKCYI ELSKQVKEKL GTIDPYFIKL ADAMVTWVEA WDEINNNKSE ETTSTKASEV AATK

References

[1]

"A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: purification, gene cloning, and trans-Golgi localization." Dhugga K.S., Tiwari S.C., Ray P.M. Proc. Natl. Acad. Sci. U.S.A. 94:7679-7684(1997) [PubMed: 9207152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-37 AND 159-173, SUBCELLULAR LOCATION, GLYCOSYLATION. Strain: cv. Early Alaska. Tissue: Stem.

[2]

"Plant polypeptides reversibly glycosylated by UDP-glucose. Possible components of Golgi beta-glucan synthase in pea cells." Dhugga K.S., Ulvskov P., Gallagher S.R., Ray P.M. J. Biol. Chem. 266:21977-21984(1991) [PubMed: 1834664] [Abstract] Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31565 mRNA. Translation: AAB88408.1.
PIR	T06507.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT75. Glycosyltransferase Family 75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004901. UPTG_synth. [Graphical view]
Pfam	PF03214. RGP. 1 hit. [Graphical view]
PIRSF	PIRSF016429. UPTG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPTG_PEA

Accession

Primary (citable) accession number: O04300

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 9, 2003
Last sequence update:	July 1, 1997
Last modified:	June 28, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program