O04294 (IMA2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Importin subunit alpha-2

Alternative name(s):
Karyopherin subunit alpha-2
Short name=KAP-alpha-2

Gene names

Name:	KAP2
Ordered Locus Names:	At4g02150
ORF Names:	T10M13.16

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs By similarity.
Subunit structure	Forms a complex with importin subunit beta-1 By similarity. Interacts with PRL1. Ref.5
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the importin alpha family. Contains 8 ARM repeats. Contains 1 IBB domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	defense response Inferred from mutant phenotype. Source: TAIR protein import into nucleus Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRL1	Q42384	3	EBI-1644689,EBI-1382964

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 531	531	Importin subunit alpha-2		PRO_0000120738
Regions
	Domain	1 – 58	58	IBB
	Repeat	111 – 153	43	ARM 1
	Repeat	154 – 198	45	ARM 2
	Repeat	199 – 236	38	ARM 3
	Repeat	237 – 281	45	ARM 4
	Repeat	282 – 321	40	ARM 5
	Repeat	322 – 364	43	ARM 6
	Repeat	365 – 405	41	ARM 7
	Repeat	406 – 447	42	ARM 8
	Compositional bias	448 – 531	84	Asp/Glu-rich (acidic)
Experimental info
	Sequence conflict	207	1	P → L in CAA70703. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04294 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 55B8979D205FCAA3	FASTA	531	58,616
	10 20 30 40 50 60 MSLRPSAKTE VRRNRYKVAV DAEEGRRRRE DNLVEIRKNK REENLQKKRF TSSMAFGSAT 70 80 90 100 110 120 GQTEQDLSSA NQLKDNLPAM VAGIWSEDSN SQLEATNLLR KLLSIEQNPP INEVVQSGVV 130 140 150 160 170 180 PRVVKFLSRD DFPKLQFEAA WALTNIASGT SENTNVIIES GAVPIFIQLL SSASEDVREQ 190 200 210 220 230 240 AVWALGNVAG DSPKCRDLVL SYGAMTPLLS QFNENTKLSM LRNATWTLSN FCRGKPPPAF 250 260 270 280 290 300 EQTQPALPVL ERLVQSMDEE VLTDACWALS YLSDNSNDKI QAVIEAGVVP RLIQLLGHSS 310 320 330 340 350 360 PSVLIPALRT IGNIVTGDDL QTQMVLDQQA LPCLLNLLKN NYKKSIKKEA CWTISNITAG 370 380 390 400 410 420 NADQIQAVID AGIIQSLVWV LQSAEFEVKK EAAWGISNAT SGGTHDQIKF MVSQGCIKPL 430 440 450 460 470 480 CDLLTCPDLK VVTVCLEALE NILVVGEAEK NLGHTGEDNL YAQMIDEAEG LEKIENLQSH 490 500 510 520 530 DNNDIYDKAV KILETFWTED NEEEGNDENH APQSGFQFGS TNVPPGQFNF I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Salchert K.D. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis." Nemeth K., Salchert K., Putnoky P., Bhalerao R., Koncz-Kalman Z., Stankovic-Stangeland B., Bako L., Mathur J., Oekresz L., Stabel S., Geigenberger P., Stitt M., Redei G.P., Schell J., Koncz C. Genes Dev. 12:3059-3073(1998) [PubMed: 9765207] [Abstract] Cited for: INTERACTION WITH PRL1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09511 mRNA. Translation: CAA70703.1. AF001308 Genomic DNA. Translation: AAC78706.1. AL161493 Genomic DNA. Translation: CAB80708.1. CP002687 Genomic DNA. Translation: AEE82131.1. AF385693 mRNA. Translation: AAK60286.1. AY081725 mRNA. Translation: AAL87378.1.
IPI	IPI00521767.
PIR	T01516.
RefSeq	NP_192124.1. NM_116447.2.
UniGene	At.5372.
3D structure databases
ProteinModelPortal	O04294.
SMR	O04294. Positions 21-502.
ModBase	Search...
Protein-protein interaction databases
IntAct	O04294. 43 interactions.
STRING	O04294.
Proteomic databases
PRIDE	O04294.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G02150.1; AT4G02150.1; AT4G02150.
GeneID	827472.
GenomeReviews	Gene locus AT4G02150 in contig CT486007_GR.
KEGG	ath:AT4G02150.
Organism-specific databases
TAIR	At4g02150.
Phylogenomic databases
eggNOG	KOG0166.
HOGENOM	HBG590318.
InParanoid	O04294.
OMA	NDENHAP.
PhylomeDB	O04294.
ProtClustDB	CLSN2916002.
Gene expression databases
ArrayExpress	O04294.
Genevestigator	O04294.
GermOnline	AT4G02150. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR002652. Importin-a_IBB. IPR024931. Importing_su_alpha. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF00514. Arm. 8 hits. PF01749. IBB. 1 hit. [Graphical view]
PIRSF	PIRSF005673. Importin_alpha. 1 hit.
SMART	SM00185. ARM. 8 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. 4 hits. PS51214. IBB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMA2_ARATH

Accession

Primary (citable) accession number: O04294
Secondary accession number(s): O04255, Q94F55

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 27, 2002
Last modified:	January 25, 2012
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program