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Protein

Importin subunit alpha-3

Gene

IMPA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to conventional NLS motifs and mediates nuclear protein import across the nuclear envelope (By similarity). Acts as cellular receptor for the nuclear import of the virD2 protein of Agrobacterium, but is not essential for Agrobacterium-mediated root transformation (PubMed:18836040). May be involved in the regulation of pathogen-induced salicylic acid accumulation (PubMed:15964279).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • defense response Source: TAIR
  • NLS-bearing protein import into nucleus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha-3Curated
Short name:
IMPa-31 Publication
Alternative name(s):
Karyopherin subunit alpha-2
Short name:
KAP-alpha-2
Protein MODIFIER OF SNC1 61 Publication
Gene namesi
Name:IMPA31 Publication
Synonyms:KAP2, MOS61 Publication
Ordered Locus Names:At4g02150Imported
ORF Names:T10M13.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02150.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytosol Source: TAIR
  • nuclear pore Source: GO_Central
  • nucleoplasm Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants show enhanced disease susceptibility to the virulent pathogen H.arabidopsidis isolate NOCO2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Importin subunit alpha-3PRO_0000120738Add
BLAST

Proteomic databases

PaxDbiO04294.
PRIDEiO04294.

Expressioni

Gene expression databases

GenevisibleiO04294. AT.

Interactioni

Subunit structurei

Forms a complex with importin subunit beta-1 (By similarity). Interacts with PRL1 (PubMed:9765207). Interacts with A.tumefaciens VirD2 and VirE2 (PubMed:18836040).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRL1Q423843EBI-1644689,EBI-1382964

Protein-protein interaction databases

BioGridi12765. 31 interactions.
IntActiO04294. 44 interactions.
STRINGi3702.AT4G02150.1.

Structurei

Secondary structure

1
531
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi93 – 10311Combined sources
Beta strandi106 – 1083Combined sources
Helixi112 – 1176Combined sources
Helixi120 – 1278Combined sources
Helixi133 – 14715Combined sources
Helixi151 – 1599Combined sources
Helixi163 – 1686Combined sources
Helixi169 – 1713Combined sources
Helixi175 – 19117Combined sources
Helixi193 – 2019Combined sources
Helixi205 – 2095Combined sources
Helixi218 – 23215Combined sources
Beta strandi234 – 2363Combined sources
Helixi240 – 2434Combined sources
Helixi246 – 2538Combined sources
Helixi259 – 27113Combined sources
Helixi277 – 28610Combined sources
Helixi289 – 2957Combined sources
Helixi301 – 31515Combined sources
Helixi319 – 3268Combined sources
Turni327 – 3293Combined sources
Helixi331 – 3399Combined sources
Helixi344 – 35714Combined sources
Helixi365 – 3706Combined sources
Helixi373 – 38210Combined sources
Helixi386 – 40116Combined sources
Helixi406 – 4138Combined sources
Helixi417 – 4226Combined sources
Helixi423 – 4253Combined sources
Helixi429 – 45022Combined sources
Helixi460 – 4678Combined sources
Helixi470 – 4767Combined sources
Helixi477 – 4793Combined sources
Helixi483 – 49614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4TNMX-ray2.90A1-531[»]
ProteinModelPortaliO04294.
SMRiO04294. Positions 34-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5858IBBPROSITE-ProRule annotationAdd
BLAST
Repeati111 – 15343ARM 1Add
BLAST
Repeati154 – 19845ARM 2Add
BLAST
Repeati199 – 23638ARM 3Add
BLAST
Repeati237 – 28145ARM 4Add
BLAST
Repeati282 – 32140ARM 5Add
BLAST
Repeati322 – 36443ARM 6Add
BLAST
Repeati365 – 40541ARM 7Add
BLAST
Repeati406 – 44742ARM 8Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi448 – 53184Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the importin alpha family.Curated
Contains 8 ARM repeats.PROSITE-ProRule annotation
Contains 1 IBB domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
HOGENOMiHOG000167616.
InParanoidiO04294.
OMAiNDENHAP.
OrthoDBiEOG09360724.
PhylomeDBiO04294.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 4 hits.
PS51214. IBB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRPSAKTE VRRNRYKVAV DAEEGRRRRE DNLVEIRKNK REENLQKKRF
60 70 80 90 100
TSSMAFGSAT GQTEQDLSSA NQLKDNLPAM VAGIWSEDSN SQLEATNLLR
110 120 130 140 150
KLLSIEQNPP INEVVQSGVV PRVVKFLSRD DFPKLQFEAA WALTNIASGT
160 170 180 190 200
SENTNVIIES GAVPIFIQLL SSASEDVREQ AVWALGNVAG DSPKCRDLVL
210 220 230 240 250
SYGAMTPLLS QFNENTKLSM LRNATWTLSN FCRGKPPPAF EQTQPALPVL
260 270 280 290 300
ERLVQSMDEE VLTDACWALS YLSDNSNDKI QAVIEAGVVP RLIQLLGHSS
310 320 330 340 350
PSVLIPALRT IGNIVTGDDL QTQMVLDQQA LPCLLNLLKN NYKKSIKKEA
360 370 380 390 400
CWTISNITAG NADQIQAVID AGIIQSLVWV LQSAEFEVKK EAAWGISNAT
410 420 430 440 450
SGGTHDQIKF MVSQGCIKPL CDLLTCPDLK VVTVCLEALE NILVVGEAEK
460 470 480 490 500
NLGHTGEDNL YAQMIDEAEG LEKIENLQSH DNNDIYDKAV KILETFWTED
510 520 530
NEEEGNDENH APQSGFQFGS TNVPPGQFNF I
Length:531
Mass (Da):58,616
Last modified:May 27, 2002 - v2
Checksum:i55B8979D205FCAA3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 2071P → L in CAA70703 (Ref. 3) Curated
Sequence conflicti330 – 3312AL → VQ in CAA75514 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15225 mRNA. Translation: CAA75514.1.
DQ087970 mRNA. Translation: AAY87936.1.
Y09511 mRNA. Translation: CAA70703.1.
AF001308 Genomic DNA. Translation: AAC78706.1.
AL161493 Genomic DNA. Translation: CAB80708.1.
CP002687 Genomic DNA. Translation: AEE82131.1.
AF385693 mRNA. Translation: AAK60286.1.
AY081725 mRNA. Translation: AAL87378.1.
PIRiT01516.
T52099.
RefSeqiNP_192124.1. NM_116447.2.
UniGeneiAt.5372.

Genome annotation databases

EnsemblPlantsiAT4G02150.1; AT4G02150.1; AT4G02150.
GeneIDi827472.
GrameneiAT4G02150.1; AT4G02150.1; AT4G02150.
KEGGiath:AT4G02150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15225 mRNA. Translation: CAA75514.1.
DQ087970 mRNA. Translation: AAY87936.1.
Y09511 mRNA. Translation: CAA70703.1.
AF001308 Genomic DNA. Translation: AAC78706.1.
AL161493 Genomic DNA. Translation: CAB80708.1.
CP002687 Genomic DNA. Translation: AEE82131.1.
AF385693 mRNA. Translation: AAK60286.1.
AY081725 mRNA. Translation: AAL87378.1.
PIRiT01516.
T52099.
RefSeqiNP_192124.1. NM_116447.2.
UniGeneiAt.5372.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4TNMX-ray2.90A1-531[»]
ProteinModelPortaliO04294.
SMRiO04294. Positions 34-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12765. 31 interactions.
IntActiO04294. 44 interactions.
STRINGi3702.AT4G02150.1.

Proteomic databases

PaxDbiO04294.
PRIDEiO04294.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02150.1; AT4G02150.1; AT4G02150.
GeneIDi827472.
GrameneiAT4G02150.1; AT4G02150.1; AT4G02150.
KEGGiath:AT4G02150.

Organism-specific databases

TAIRiAT4G02150.

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
HOGENOMiHOG000167616.
InParanoidiO04294.
OMAiNDENHAP.
OrthoDBiEOG09360724.
PhylomeDBiO04294.

Miscellaneous databases

PROiO04294.

Gene expression databases

GenevisibleiO04294. AT.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 4 hits.
PS51214. IBB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMPA3_ARATH
AccessioniPrimary (citable) accession number: O04294
Secondary accession number(s): O04255
, O49601, Q4JHM3, Q94F55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 27, 2002
Last modified: September 7, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.