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Protein

Importin subunit alpha-3

Gene

IMPA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to conventional NLS motifs and mediates nuclear protein import across the nuclear envelope (By similarity). Acts as cellular receptor for the nuclear import of the virD2 protein of Agrobacterium, but is not essential for Agrobacterium-mediated root transformation (PubMed:18836040). May be involved in the regulation of pathogen-induced salicylic acid accumulation (PubMed:15964279).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • defense response Source: TAIR
  • NLS-bearing protein import into nucleus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha-3Curated
Short name:
IMPa-31 Publication
Alternative name(s):
Karyopherin subunit alpha-2
Short name:
KAP-alpha-2
Protein MODIFIER OF SNC1 61 Publication
Gene namesi
Name:IMPA31 Publication
Synonyms:KAP2, MOS61 Publication
Ordered Locus Names:At4g02150Imported
ORF Names:T10M13.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G02150.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytosol Source: TAIR
  • nuclear pore Source: GO_Central
  • nucleoplasm Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants show enhanced disease susceptibility to the virulent pathogen H.arabidopsidis isolate NOCO2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207381 – 531Importin subunit alpha-3Add BLAST531

Proteomic databases

PaxDbiO04294.
PRIDEiO04294.

Expressioni

Gene expression databases

GenevisibleiO04294. AT.

Interactioni

Subunit structurei

Forms a complex with importin subunit beta-1 (By similarity). Interacts with PRL1 (PubMed:9765207). Interacts with A.tumefaciens VirD2 and VirE2 (PubMed:18836040).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRL1Q423843EBI-1644689,EBI-1382964

Protein-protein interaction databases

BioGridi12765. 31 interactors.
IntActiO04294. 44 interactors.
STRINGi3702.AT4G02150.1.

Structurei

Secondary structure

1531
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi93 – 103Combined sources11
Beta strandi106 – 108Combined sources3
Helixi112 – 117Combined sources6
Helixi120 – 127Combined sources8
Helixi133 – 147Combined sources15
Helixi151 – 159Combined sources9
Helixi163 – 168Combined sources6
Helixi169 – 171Combined sources3
Helixi175 – 191Combined sources17
Helixi193 – 201Combined sources9
Helixi205 – 209Combined sources5
Helixi218 – 232Combined sources15
Beta strandi234 – 236Combined sources3
Helixi240 – 243Combined sources4
Helixi246 – 253Combined sources8
Helixi259 – 271Combined sources13
Helixi277 – 286Combined sources10
Helixi289 – 295Combined sources7
Helixi301 – 315Combined sources15
Helixi319 – 326Combined sources8
Turni327 – 329Combined sources3
Helixi331 – 339Combined sources9
Helixi344 – 357Combined sources14
Helixi365 – 370Combined sources6
Helixi373 – 382Combined sources10
Helixi386 – 401Combined sources16
Helixi406 – 413Combined sources8
Helixi417 – 422Combined sources6
Helixi423 – 425Combined sources3
Helixi429 – 450Combined sources22
Helixi460 – 467Combined sources8
Helixi470 – 476Combined sources7
Helixi477 – 479Combined sources3
Helixi483 – 496Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TNMX-ray2.90A1-531[»]
ProteinModelPortaliO04294.
SMRiO04294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58IBBPROSITE-ProRule annotationAdd BLAST58
Repeati111 – 153ARM 1Add BLAST43
Repeati154 – 198ARM 2Add BLAST45
Repeati199 – 236ARM 3Add BLAST38
Repeati237 – 281ARM 4Add BLAST45
Repeati282 – 321ARM 5Add BLAST40
Repeati322 – 364ARM 6Add BLAST43
Repeati365 – 405ARM 7Add BLAST41
Repeati406 – 447ARM 8Add BLAST42

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi448 – 531Asp/Glu-rich (acidic)Add BLAST84

Sequence similaritiesi

Belongs to the importin alpha family.Curated
Contains 8 ARM repeats.PROSITE-ProRule annotation
Contains 1 IBB domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
HOGENOMiHOG000167616.
InParanoidiO04294.
OMAiNDENHAP.
OrthoDBiEOG09360724.
PhylomeDBiO04294.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 4 hits.
PS51214. IBB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRPSAKTE VRRNRYKVAV DAEEGRRRRE DNLVEIRKNK REENLQKKRF
60 70 80 90 100
TSSMAFGSAT GQTEQDLSSA NQLKDNLPAM VAGIWSEDSN SQLEATNLLR
110 120 130 140 150
KLLSIEQNPP INEVVQSGVV PRVVKFLSRD DFPKLQFEAA WALTNIASGT
160 170 180 190 200
SENTNVIIES GAVPIFIQLL SSASEDVREQ AVWALGNVAG DSPKCRDLVL
210 220 230 240 250
SYGAMTPLLS QFNENTKLSM LRNATWTLSN FCRGKPPPAF EQTQPALPVL
260 270 280 290 300
ERLVQSMDEE VLTDACWALS YLSDNSNDKI QAVIEAGVVP RLIQLLGHSS
310 320 330 340 350
PSVLIPALRT IGNIVTGDDL QTQMVLDQQA LPCLLNLLKN NYKKSIKKEA
360 370 380 390 400
CWTISNITAG NADQIQAVID AGIIQSLVWV LQSAEFEVKK EAAWGISNAT
410 420 430 440 450
SGGTHDQIKF MVSQGCIKPL CDLLTCPDLK VVTVCLEALE NILVVGEAEK
460 470 480 490 500
NLGHTGEDNL YAQMIDEAEG LEKIENLQSH DNNDIYDKAV KILETFWTED
510 520 530
NEEEGNDENH APQSGFQFGS TNVPPGQFNF I
Length:531
Mass (Da):58,616
Last modified:May 27, 2002 - v2
Checksum:i55B8979D205FCAA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207P → L in CAA70703 (Ref. 3) Curated1
Sequence conflicti330 – 331AL → VQ in CAA75514 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15225 mRNA. Translation: CAA75514.1.
DQ087970 mRNA. Translation: AAY87936.1.
Y09511 mRNA. Translation: CAA70703.1.
AF001308 Genomic DNA. Translation: AAC78706.1.
AL161493 Genomic DNA. Translation: CAB80708.1.
CP002687 Genomic DNA. Translation: AEE82131.1.
AF385693 mRNA. Translation: AAK60286.1.
AY081725 mRNA. Translation: AAL87378.1.
PIRiT01516.
T52099.
RefSeqiNP_192124.1. NM_116447.3.
UniGeneiAt.5372.

Genome annotation databases

EnsemblPlantsiAT4G02150.1; AT4G02150.1; AT4G02150.
GeneIDi827472.
GrameneiAT4G02150.1; AT4G02150.1; AT4G02150.
KEGGiath:AT4G02150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y15225 mRNA. Translation: CAA75514.1.
DQ087970 mRNA. Translation: AAY87936.1.
Y09511 mRNA. Translation: CAA70703.1.
AF001308 Genomic DNA. Translation: AAC78706.1.
AL161493 Genomic DNA. Translation: CAB80708.1.
CP002687 Genomic DNA. Translation: AEE82131.1.
AF385693 mRNA. Translation: AAK60286.1.
AY081725 mRNA. Translation: AAL87378.1.
PIRiT01516.
T52099.
RefSeqiNP_192124.1. NM_116447.3.
UniGeneiAt.5372.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TNMX-ray2.90A1-531[»]
ProteinModelPortaliO04294.
SMRiO04294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12765. 31 interactors.
IntActiO04294. 44 interactors.
STRINGi3702.AT4G02150.1.

Proteomic databases

PaxDbiO04294.
PRIDEiO04294.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G02150.1; AT4G02150.1; AT4G02150.
GeneIDi827472.
GrameneiAT4G02150.1; AT4G02150.1; AT4G02150.
KEGGiath:AT4G02150.

Organism-specific databases

TAIRiAT4G02150.

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
HOGENOMiHOG000167616.
InParanoidiO04294.
OMAiNDENHAP.
OrthoDBiEOG09360724.
PhylomeDBiO04294.

Miscellaneous databases

PROiO04294.

Gene expression databases

GenevisibleiO04294. AT.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 4 hits.
PS51214. IBB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMPA3_ARATH
AccessioniPrimary (citable) accession number: O04294
Secondary accession number(s): O04255
, O49601, Q4JHM3, Q94F55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.