ID   LDOX_PERFR              Reviewed;         362 AA.
AC   O04274;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   05-MAY-2009, entry version 43.
DE   RecName: Full=Leucoanthocyanidin dioxygenase;
DE            Short=LDOX;
DE            Short=Leucocyanidin oxygenase;
DE            EC=1.14.11.19;
DE   AltName: Full=Leucoanthocyanidin hydroxylase;
GN   Name=ANS;
OS   Perilla frutescens (Beefsteak plant) (Chinese basil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Elsholtzieae;
OC   Perilla.
OX   NCBI_TaxID=48386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, AND TISSUE SPECIFICITY.
RC   TISSUE=Leaf;
RX   MEDLINE=99174284; PubMed=10074715;
RX   DOI=10.1046/j.1365-313X.1999.00365.x;
RA   Saito K., Kobayashi M., Gong Z., Tanaka Y., Yamazaki M.;
RT   "Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-
RT   dependent oxygenase: molecular cloning and functional expression of
RT   cDNA from a red forma of Perilla frutescens.";
RL   Plant J. 17:181-189(1999).
CC   -!- FUNCTION: Oxidation of leucoanthocyanidins into anthocyanidins.
CC   -!- CATALYTIC ACTIVITY: Leucocyanidin + 2-oxoglutarate + O(2) = cis-
CC       and trans-dihydroquercetins + succinate + CO(2) + H(2)O.
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 ascorbate molecule per subunit (By similarity).
CC   -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC   -!- TISSUE SPECIFICITY: Expressed in red but not in green forma of
CC       P.frutescens. In red forma, it is predominantly expressed in stems
CC       and leaves, but not in roots.
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; AB003779; BAA20143.1; -; mRNA.
DR   HSSP; Q96323; 1GP5.
DR   SMR; O04274; 11-356.
DR   BRENDA; 1.14.11.19; 142935.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0050589; F:leucocyanidin oxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; Oxoglutarate/Fe-dep_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    362       Leucoanthocyanidin dioxygenase.
FT                                /FTId=PRO_0000067302.
FT   ACT_SITE    304    304       Potential.
FT   METAL       238    238       Iron (By similarity).
FT   METAL       240    240       Iron (By similarity).
FT   METAL       294    294       Iron (By similarity).
SQ   SEQUENCE   362 AA;  40826 MW;  7A03B4E86F91E1EA CRC64;
     MVTSAMGPSP RVEELARSGL DTIPKDYVRP EEELKSIIGN ILAEEKSSEG PQLPTIDLEE
     MDSRDEEGRK KCHEELKKAA TDWGVMHLIN HGIPEELIDR VKAAGKEFFE LPVEEKEAYA
     NDQAAGNVQG YGSKLANNAS GQLEWEDYFF HCVYPEHKTD LSIWPTKPPD YIPATSEYAK
     QLRALATKIL SVLSIGLGLE KGRLEKEVGG AEDLIVQMKI NFYPKCPQPE LALGWEAHTD
     VSALTFILHN MVPGLQLFYE DKWVTAKCVP NSIIMHIGDT LEILSNGKYK SILHRGLVNK
     EKVRISWAVF CEPPKEKIVL QPLPETVSEV EPPRFPPRTF AQHLKHKLFR KTDGDLDEKP
     TY
//