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O04274

- LDOX_PERFR

UniProt

O04274 - LDOX_PERFR

Protein

Leucoanthocyanidin dioxygenase

Gene

ANS

Organism
Perilla frutescens (Beefsteak mint) (Perilla ocymoides)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    • Comment

    Functioni

    Oxidation of leucoanthocyanidins into anthocyanidins.

    Catalytic activityi

    Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O.1 Publication

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 ascorbate molecule per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi238 – 2381IronPROSITE-ProRule annotation
    Metal bindingi240 – 2401IronPROSITE-ProRule annotation
    Metal bindingi294 – 2941IronPROSITE-ProRule annotation
    Active sitei304 – 3041Sequence Analysis

    GO - Molecular functioni

    1. L-ascorbic acid binding Source: UniProtKB-KW
    2. leucocyanidin oxygenase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. anthocyanin-containing compound biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding, Vitamin C

    Enzyme and pathway databases

    UniPathwayiUPA00009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucoanthocyanidin dioxygenase (EC:1.14.11.19)
    Short name:
    LDOX
    Short name:
    Leucocyanidin oxygenase
    Alternative name(s):
    Leucoanthocyanidin hydroxylase
    Gene namesi
    Name:ANS
    OrganismiPerilla frutescens (Beefsteak mint) (Perilla ocymoides)
    Taxonomic identifieri48386 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeElsholtzieaePerilla

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 362362Leucoanthocyanidin dioxygenasePRO_0000067302Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in red but not in green forma of P.frutescens. In red forma, it is predominantly expressed in stems and leaves, but not in roots.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO04274.
    SMRiO04274. Positions 11-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini214 – 313100Fe2OG dioxygenasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.60.120.330. 1 hit.
    InterProiIPR026992. DIOX_N.
    IPR027443. IPNS-like.
    IPR005123. Oxoglu/Fe-dep_dioxygenase.
    [Graphical view]
    PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
    PF14226. DIOX_N. 1 hit.
    [Graphical view]
    PROSITEiPS51471. FE2OG_OXY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O04274-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTSAMGPSP RVEELARSGL DTIPKDYVRP EEELKSIIGN ILAEEKSSEG    50
    PQLPTIDLEE MDSRDEEGRK KCHEELKKAA TDWGVMHLIN HGIPEELIDR 100
    VKAAGKEFFE LPVEEKEAYA NDQAAGNVQG YGSKLANNAS GQLEWEDYFF 150
    HCVYPEHKTD LSIWPTKPPD YIPATSEYAK QLRALATKIL SVLSIGLGLE 200
    KGRLEKEVGG AEDLIVQMKI NFYPKCPQPE LALGWEAHTD VSALTFILHN 250
    MVPGLQLFYE DKWVTAKCVP NSIIMHIGDT LEILSNGKYK SILHRGLVNK 300
    EKVRISWAVF CEPPKEKIVL QPLPETVSEV EPPRFPPRTF AQHLKHKLFR 350
    KTDGDLDEKP TY 362
    Length:362
    Mass (Da):40,826
    Last modified:July 1, 1997 - v1
    Checksum:i7A03B4E86F91E1EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003779 mRNA. Translation: BAA20143.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003779 mRNA. Translation: BAA20143.1 .

    3D structure databases

    ProteinModelPortali O04274.
    SMRi O04274. Positions 11-356.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00009 .

    Family and domain databases

    Gene3Di 2.60.120.330. 1 hit.
    InterProi IPR026992. DIOX_N.
    IPR027443. IPNS-like.
    IPR005123. Oxoglu/Fe-dep_dioxygenase.
    [Graphical view ]
    Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
    PF14226. DIOX_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51471. FE2OG_OXY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens."
      Saito K., Kobayashi M., Gong Z., Tanaka Y., Yamazaki M.
      Plant J. 17:181-189(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY.
      Tissue: Leaf.

    Entry informationi

    Entry nameiLDOX_PERFR
    AccessioniPrimary (citable) accession number: O04274
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3