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Protein

Leucoanthocyanidin dioxygenase

Gene

ANS

Organism
Perilla frutescens (Beefsteak mint) (Perilla ocymoides)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Oxidation of leucoanthocyanidins into anthocyanidins.

Catalytic activityi

Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • L-ascorbateBy similarityNote: Binds 1 ascorbate molecule per subunit.By similarity

Pathwayi: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi238IronPROSITE-ProRule annotation1
Metal bindingi240IronPROSITE-ProRule annotation1
Metal bindingi294IronPROSITE-ProRule annotation1
Active sitei304Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Flavonoid biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

UniPathwayiUPA00009.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucoanthocyanidin dioxygenase (EC:1.14.11.19)
Short name:
LDOX
Short name:
Leucocyanidin oxygenase
Alternative name(s):
Leucoanthocyanidin hydroxylase
Gene namesi
Name:ANS
OrganismiPerilla frutescens (Beefsteak mint) (Perilla ocymoides)
Taxonomic identifieri48386 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeElsholtzieaePerilla

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000673021 – 362Leucoanthocyanidin dioxygenaseAdd BLAST362

Expressioni

Tissue specificityi

Expressed in red but not in green forma of P.frutescens. In red forma, it is predominantly expressed in stems and leaves, but not in roots.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO04274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini214 – 313Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST100

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK05277.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04274-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTSAMGPSP RVEELARSGL DTIPKDYVRP EEELKSIIGN ILAEEKSSEG
60 70 80 90 100
PQLPTIDLEE MDSRDEEGRK KCHEELKKAA TDWGVMHLIN HGIPEELIDR
110 120 130 140 150
VKAAGKEFFE LPVEEKEAYA NDQAAGNVQG YGSKLANNAS GQLEWEDYFF
160 170 180 190 200
HCVYPEHKTD LSIWPTKPPD YIPATSEYAK QLRALATKIL SVLSIGLGLE
210 220 230 240 250
KGRLEKEVGG AEDLIVQMKI NFYPKCPQPE LALGWEAHTD VSALTFILHN
260 270 280 290 300
MVPGLQLFYE DKWVTAKCVP NSIIMHIGDT LEILSNGKYK SILHRGLVNK
310 320 330 340 350
EKVRISWAVF CEPPKEKIVL QPLPETVSEV EPPRFPPRTF AQHLKHKLFR
360
KTDGDLDEKP TY
Length:362
Mass (Da):40,826
Last modified:July 1, 1997 - v1
Checksum:i7A03B4E86F91E1EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003779 mRNA. Translation: BAA20143.1.

Genome annotation databases

KEGGiag:BAA20143.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003779 mRNA. Translation: BAA20143.1.

3D structure databases

ProteinModelPortaliO04274.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAA20143.

Phylogenomic databases

KOiK05277.

Enzyme and pathway databases

UniPathwayiUPA00009.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDOX_PERFR
AccessioniPrimary (citable) accession number: O04274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 1, 1997
Last modified: October 5, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.