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O04274

- LDOX_PERFR

UniProt

O04274 - LDOX_PERFR

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Protein

Leucoanthocyanidin dioxygenase

Gene
ANS
Organism
Perilla frutescens (Beefsteak mint) (Perilla ocymoides)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Oxidation of leucoanthocyanidins into anthocyanidins.

Catalytic activityi

Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O.1 Publication

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 1 ascorbate molecule per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi238 – 2381Iron By similarity
Metal bindingi240 – 2401Iron By similarity
Metal bindingi294 – 2941Iron By similarity
Active sitei304 – 3041 Reviewed prediction

GO - Molecular functioni

  1. L-ascorbic acid binding Source: UniProtKB-KW
  2. leucocyanidin oxygenase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. anthocyanin-containing compound biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Flavonoid biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

UniPathwayiUPA00009.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucoanthocyanidin dioxygenase (EC:1.14.11.19)
Short name:
LDOX
Short name:
Leucocyanidin oxygenase
Alternative name(s):
Leucoanthocyanidin hydroxylase
Gene namesi
Name:ANS
OrganismiPerilla frutescens (Beefsteak mint) (Perilla ocymoides)
Taxonomic identifieri48386 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeElsholtzieaePerilla

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Leucoanthocyanidin dioxygenasePRO_0000067302Add
BLAST

Expressioni

Tissue specificityi

Expressed in red but not in green forma of P.frutescens. In red forma, it is predominantly expressed in stems and leaves, but not in roots.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO04274.
SMRiO04274. Positions 11-356.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini214 – 313100Fe2OG dioxygenaseAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04274-1 [UniParc]FASTAAdd to Basket

« Hide

MVTSAMGPSP RVEELARSGL DTIPKDYVRP EEELKSIIGN ILAEEKSSEG    50
PQLPTIDLEE MDSRDEEGRK KCHEELKKAA TDWGVMHLIN HGIPEELIDR 100
VKAAGKEFFE LPVEEKEAYA NDQAAGNVQG YGSKLANNAS GQLEWEDYFF 150
HCVYPEHKTD LSIWPTKPPD YIPATSEYAK QLRALATKIL SVLSIGLGLE 200
KGRLEKEVGG AEDLIVQMKI NFYPKCPQPE LALGWEAHTD VSALTFILHN 250
MVPGLQLFYE DKWVTAKCVP NSIIMHIGDT LEILSNGKYK SILHRGLVNK 300
EKVRISWAVF CEPPKEKIVL QPLPETVSEV EPPRFPPRTF AQHLKHKLFR 350
KTDGDLDEKP TY 362
Length:362
Mass (Da):40,826
Last modified:July 1, 1997 - v1
Checksum:i7A03B4E86F91E1EA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB003779 mRNA. Translation: BAA20143.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB003779 mRNA. Translation: BAA20143.1 .

3D structure databases

ProteinModelPortali O04274.
SMRi O04274. Positions 11-356.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00009 .

Family and domain databases

Gene3Di 2.60.120.330. 1 hit.
InterProi IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view ]
Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view ]
PROSITEi PS51471. FE2OG_OXY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens."
    Saito K., Kobayashi M., Gong Z., Tanaka Y., Yamazaki M.
    Plant J. 17:181-189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Leaf.

Entry informationi

Entry nameiLDOX_PERFR
AccessioniPrimary (citable) accession number: O04274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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