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O04274 (LDOX_PERFR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucoanthocyanidin dioxygenase

Short name=LDOX
Short name=Leucocyanidin oxygenase
EC=1.14.11.19
Alternative name(s):
Leucoanthocyanidin hydroxylase
Gene names
Name:ANS
OrganismPerilla frutescens (Beefsteak mint) (Perilla ocymoides)
Taxonomic identifier48386 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeElsholtzieaePerilla

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Oxidation of leucoanthocyanidins into anthocyanidins.

Catalytic activity

Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O. Ref.1

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 ascorbate molecule per subunit By similarity.

Pathway

Pigment biosynthesis; anthocyanin biosynthesis.

Tissue specificity

Expressed in red but not in green forma of P.frutescens. In red forma, it is predominantly expressed in stems and leaves, but not in roots. Ref.1

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family.

Contains 1 Fe2OG dioxygenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Leucoanthocyanidin dioxygenase
PRO_0000067302

Regions

Domain214 – 313100Fe2OG dioxygenase

Sites

Active site3041 Potential
Metal binding2381Iron By similarity
Metal binding2401Iron By similarity
Metal binding2941Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
O04274 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 7A03B4E86F91E1EA

FASTA36240,826
        10         20         30         40         50         60 
MVTSAMGPSP RVEELARSGL DTIPKDYVRP EEELKSIIGN ILAEEKSSEG PQLPTIDLEE 

        70         80         90        100        110        120 
MDSRDEEGRK KCHEELKKAA TDWGVMHLIN HGIPEELIDR VKAAGKEFFE LPVEEKEAYA 

       130        140        150        160        170        180 
NDQAAGNVQG YGSKLANNAS GQLEWEDYFF HCVYPEHKTD LSIWPTKPPD YIPATSEYAK 

       190        200        210        220        230        240 
QLRALATKIL SVLSIGLGLE KGRLEKEVGG AEDLIVQMKI NFYPKCPQPE LALGWEAHTD 

       250        260        270        280        290        300 
VSALTFILHN MVPGLQLFYE DKWVTAKCVP NSIIMHIGDT LEILSNGKYK SILHRGLVNK 

       310        320        330        340        350        360 
EKVRISWAVF CEPPKEKIVL QPLPETVSEV EPPRFPPRTF AQHLKHKLFR KTDGDLDEKP 


TY 

« Hide

References

[1]"Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens."
Saito K., Kobayashi M., Gong Z., Tanaka Y., Yamazaki M.
Plant J. 17:181-189(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB003779 mRNA. Translation: BAA20143.1.

3D structure databases

ProteinModelPortalO04274.
SMRO04274. Positions 11-356.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00009.

Family and domain databases

Gene3D2.60.120.330. 1 hit.
InterProIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLDOX_PERFR
AccessionPrimary (citable) accession number: O04274
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways