Reviewed, UniProtKB/Swiss-Prot O04274 (LDOX_PERFR)

Names and origin

Protein names

Recommended name:
    Leucoanthocyanidin dioxygenase
      Short name=LDOX
      Short name=Leucocyanidin oxygenase
    EC=1.14.11.19
Alternative name(s):
    Leucoanthocyanidin hydroxylase

Gene names

Name:

ANS

Organism

Perilla frutescens (Beefsteak plant) (Chinese basil)

Taxonomic identifier

48386 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Lamiaceae › Nepetoideae › Elsholtzieae › Perilla

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidation of leucoanthocyanidins into anthocyanidins.
Catalytic activity	Leucocyanidin + 2-oxoglutarate + O2 = cis- and trans-dihydroquercetins + succinate + CO2 + H2O. Ref.1
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Tissue specificity	Expressed in red but not in green forma of P.frutescens. In red forma, it is predominantly expressed in stems and leaves, but not in roots. Ref.1
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW leucocyanidin oxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Leucoanthocyanidin dioxygenase		PRO_0000067302
Sites
	Active site	304	1	Potential
	Metal binding	238	1	Iron By similarity
	Metal binding	240	1	Iron By similarity
	Metal binding	294	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O04274-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7A03B4E86F91E1EA	FASTA	362	40,826
	10 20 30 40 50 60 MVTSAMGPSP RVEELARSGL DTIPKDYVRP EEELKSIIGN ILAEEKSSEG PQLPTIDLEE 70 80 90 100 110 120 MDSRDEEGRK KCHEELKKAA TDWGVMHLIN HGIPEELIDR VKAAGKEFFE LPVEEKEAYA 130 140 150 160 170 180 NDQAAGNVQG YGSKLANNAS GQLEWEDYFF HCVYPEHKTD LSIWPTKPPD YIPATSEYAK 190 200 210 220 230 240 QLRALATKIL SVLSIGLGLE KGRLEKEVGG AEDLIVQMKI NFYPKCPQPE LALGWEAHTD 250 260 270 280 290 300 VSALTFILHN MVPGLQLFYE DKWVTAKCVP NSIIMHIGDT LEILSNGKYK SILHRGLVNK 310 320 330 340 350 360 EKVRISWAVF CEPPKEKIVL QPLPETVSEV EPPRFPPRTF AQHLKHKLFR KTDGDLDEKP TY

References

[1]

"Direct evidence for anthocyanidin synthase as a 2-oxoglutarate-dependent oxygenase: molecular cloning and functional expression of cDNA from a red forma of Perilla frutescens." Saito K., Kobayashi M., Gong Z., Tanaka Y., Yamazaki M. Plant J. 17:181-189(1999) [PubMed: 10074715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003779 mRNA. Translation: BAA20143.1.
3D structure databases
HSSP	HSSP built from PDB template 1GP5 based on UniProtKB Q96323.
SMR	O04274. Positions 11-356.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.11.19. 142935.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDOX_PERFR

Accession

Primary (citable) accession number: O04274

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 1, 1997
Last modified:	September 22, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)