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Protein

Delta-1-pyrroline-5-carboxylate synthase 1

Gene

P5CS1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants. Involved in abiotic stress tolerance.1 Publication

Miscellaneous

Tobacco plants over-expressing P5CS1 and P5CS2 have elevated proline levels and display enhanced abiotic stress tolerance.1 Publication

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulationi

Feedback regulated by proline.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Delta-1-pyrroline-5-carboxylate synthase 2 (P5CS2), Delta-1-pyrroline-5-carboxylate synthase 1 (P5CS1), Delta-1-pyrroline-5-carboxylate synthase (OsJ_18779)
  2. Delta-1-pyrroline-5-carboxylate synthase 2 (P5CS2), Delta-1-pyrroline-5-carboxylate synthase 1 (P5CS1), Delta-1-pyrroline-5-carboxylate synthase (OsJ_18779)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60SubstrateBy similarity1
Binding sitei157SubstrateBy similarity1
Binding sitei176Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi196 – 197ATPBy similarity2
Nucleotide bindingi202 – 207ATPBy similarity6
Nucleotide bindingi236 – 242ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis, Stress response
LigandATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00098; UER00359
UPA00098; UER00360

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase 1Curated
Short name:
OsP5CS11 Publication
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:P5CS11 Publication
Synonyms:P5CS1 Publication
Ordered Locus Names:Os05g0455500, LOC_Os05g38150
ORF Names:OJ1651_D06.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 5

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097771 – 716Delta-1-pyrroline-5-carboxylate synthase 1Add BLAST716

Proteomic databases

PaxDbiO04226

Expressioni

Tissue specificityi

Expressed at high levels in leaves.1 Publication

Inductioni

Induced by salt, drought and cold stresses, and abscisic acid (ABA).1 Publication

Gene expression databases

ExpressionAtlasiO04226 differential
GenevisibleiO04226 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os05g38150.1

Structurei

3D structure databases

ProteinModelPortaliO04226
SMRiO04226
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 296Glutamate 5-kinaseAdd BLAST296
Regioni297 – 716Gamma-glutamyl phosphate reductaseAdd BLAST420

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

Phylogenomic databases

eggNOGiKOG1154 Eukaryota
KOG4165 Eukaryota
COG0014 LUCA
COG0263 LUCA
InParanoidiO04226
KOiK12657
OMAiMYVDSEA
OrthoDBiEOG093605RS

Family and domain databases

CDDicd07079 ALDH_F18-19_ProA-GPR, 1 hit
Gene3Di3.40.1160.10, 1 hit
3.40.309.10, 1 hit
3.40.605.10, 2 hits
HAMAPiMF_00412 ProA, 1 hit
MF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
IPR001048 Asp/Glu/Uridylate_kinase
IPR020593 G-glutamylP_reductase_CS
IPR001057 Glu/AcGlu_kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR000965 GPR_dom
IPR005766 P5_carboxy_syn
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF00171 Aldedh, 1 hit
PIRSFiPIRSF036429 P5C_syn, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
SSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR01092 P5CS, 1 hit
TIGR00407 proA, 1 hit
TIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS01223 PROA, 1 hit

Sequencei

Sequence statusi: Complete.

O04226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS
60 70 80 90 100
LGYEVILVTS GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ
110 120 130 140 150
SGLMALYDML FNQLDVSSSQ LLVTDSDFEN PKFREQLTET VESLLDLKVI
160 170 180 190 200
PIFNENDAIS TRKAPYEDSS GIFWDNDSLA GLLALELKAD LLILLSDVDG
210 220 230 240 250
LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT AKVKAAVLAS
260 270 280 290 300
NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV
310 320 330 340 350
AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG
360 370 380 390 400
YEKPLVARLT IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK
410 420 430 440 450
TSCPLGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE AIRSNTILHK
460 470 480 490 500
VITDAIPRNV GEKLIGLVTT RDEIADLLKL DDVIDLVIPR GSNKLVSQIK
510 520 530 540 550
ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP AACNAMETLL
560 570 580 590 600
VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM
610 620 630 640 650
ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH
660 670 680 690 700
NASTRFSDGA RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV
710
NGDKDVVYTH KSLPLQ
Length:716
Mass (Da):77,745
Last modified:May 30, 2006 - v2
Checksum:iF8D3C7D9449CFA64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173F → S in AAS89034 (Ref. 2) Curated1
Sequence conflicti488I → T in BAA19916 (PubMed:9106509).Curated1
Sequence conflicti500K → E in AAS89034 (Ref. 2) Curated1
Sequence conflicti530H → L in BAA19916 (PubMed:9106509).Curated1
Sequence conflicti537I → T in BAA19916 (PubMed:9106509).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49714 mRNA Translation: BAA19916.1
AY574031 mRNA Translation: AAS89034.1
AC111016 Genomic DNA Translation: AAU90213.1
AP008211 Genomic DNA Translation: BAF17637.1
AP014961 Genomic DNA Translation: BAS94357.1
AK102633 mRNA Translation: BAG95649.1
PIRiT03695
RefSeqiXP_015640176.1, XM_015784690.1
UniGeneiOs.12281

Genome annotation databases

EnsemblPlantsiOs05t0455500-02; Os05t0455500-02; Os05g0455500
GeneIDi4338979
GrameneiOs05t0455500-02; Os05t0455500-02; Os05g0455500
KEGGiosa:4338979

Similar proteinsi

Entry informationi

Entry nameiP5CS1_ORYSJ
AccessioniPrimary (citable) accession number: O04226
Secondary accession number(s): Q0DHN6, Q60EM4, Q6PW76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2006
Last modified: May 23, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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