Delta-1-pyrroline-5-carboxylate synthase - Oryza sativa subsp. japonica (Rice)

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O04226 (P5CS_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name=P5CS

Including the following 2 domains:

Glutamate 5-kinase
Short name=GK
EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase

Gene names

Name:	P5CS
Ordered Locus Names:	Os05g0455500, LOC_Os05g38150
ORF Names:	OJ1651_D06.9

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. L-glutamate 5-semialdehyde + phosphate + NADP⁺ = L-glutamyl 5-phosphate + NADPH.
Enzyme regulation	Feedback regulated by proline.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Tissue specificity	Expressed at high levels in leaves and is inducible in roots subjected to salt stress.
Sequence similarities	In the N-terminal section; belongs to the glutamate 5-kinase family. In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway embryo development ending in seed dormancy Inferred from electronic annotation. Source: EnsemblPlants/Gramene hyperosmotic salinity response Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to abscisic acid stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to desiccation Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to oxidative stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene root development Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: EC glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 716

716

Delta-1-pyrroline-5-carboxylate synthase

PRO_0000109777

Regions

Nucleotide binding

196 – 197

ATP By similarity

Nucleotide binding

236 – 242

ATP By similarity

Region

1 – 296

296

Glutamate 5-kinase

Region

297 – 716

420

Gamma-glutamyl phosphate reductase

Sites

Binding site

Substrate By similarity

Binding site

157

Substrate By similarity

Binding site

176

Substrate; via amide nitrogen By similarity

Experimental info

Sequence conflict

173

F → S in AAS89034. Ref.2

Sequence conflict

488

I → T in BAA19916. Ref.1

Sequence conflict

500

K → E in AAS89034. Ref.2

Sequence conflict

530

H → L in BAA19916. Ref.1

Sequence conflict

537

I → T in BAA19916. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O04226 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: F8D3C7D9449CFA64
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FASTA

716

77,745

        10         20         30         40         50         60 
MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS LGYEVILVTS 

        70         80         90        100        110        120 
GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ SGLMALYDML FNQLDVSSSQ 

       130        140        150        160        170        180 
LLVTDSDFEN PKFREQLTET VESLLDLKVI PIFNENDAIS TRKAPYEDSS GIFWDNDSLA 

       190        200        210        220        230        240 
GLLALELKAD LLILLSDVDG LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT 

       250        260        270        280        290        300 
AKVKAAVLAS NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV 

       310        320        330        340        350        360 
AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG YEKPLVARLT 

       370        380        390        400        410        420 
IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK TSCPLGVLLI VFESRPDALV 

       430        440        450        460        470        480 
QIASLAIRSG NGLLLKGGKE AIRSNTILHK VITDAIPRNV GEKLIGLVTT RDEIADLLKL 

       490        500        510        520        530        540 
DDVIDLVIPR GSNKLVSQIK ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP 

       550        560        570        580        590        600 
AACNAMETLL VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM 

       610        620        630        640        650        660 
ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH NASTRFSDGA 

       670        680        690        700        710 
RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV NGDKDVVYTH KSLPLQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L." Igarashi Y., Yoshiba Y., Sanada Y., Yamaguchi-Shinozaki K., Wada K., Shinozaki K. Plant Mol. Biol. 33:857-865(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Akibare.
[2]	"Nucleotide sequence of delta-1-pyrroline-5-carboxylate synthetase." Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Tainung 71.
[3]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49714 mRNA. Translation: BAA19916.1. AY574031 mRNA. Translation: AAS89034.1. AC111016 Genomic DNA. Translation: AAU90213.1.
PIR	T03695.
RefSeq	NP_001055723.1. NM_001062258.1.
UniGene	Os.12281.
3D structure databases
ProteinModelPortal	O04226.
ModBase	Search...
Protein-protein interaction databases
STRING	39946.BGIOSIBCE019273.
Proteomic databases
PRIDE	O04226.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g38150.1; LOC_Os05g38150.1; LOC_Os05g38150. LOC_Os05g38150.2; LOC_Os05g38150.2; LOC_Os05g38150.
GeneID	4338979.
KEGG	dosa:Os05t0455500-02. osa:4338979.
Organism-specific databases
Gramene	O04226.
Phylogenomic databases
eggNOG	COG0014.
KO	K12657.
OMA	LLPWVQS.
ProtClustDB	PLN02418.
Enzyme and pathway databases
UniPathway	UPA00098; UER00359. UPA00098; UER00360.
Gene expression databases
ArrayExpress	O04226.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit. 3.40.309.10. 1 hit. 3.40.605.10. 2 hits.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR001048. Asp/Glu/Uridylate_kinase. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR001057. Glu/AcGlu_kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR005766. P5_carboxy_syn. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF036429. P5C_syn. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01092. P5CS. 1 hit. TIGR00407. proA. 1 hit. TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CS_ORYSJ

Accession

Primary (citable) accession number: O04226
Secondary accession number(s): Q60EM4, Q6PW76

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2006
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents