Reviewed, UniProtKB/Swiss-Prot O04226 (P5CS_ORYSJ)

Names and origin

Protein names

Recommended name:
    Delta-1-pyrroline-5-carboxylate synthetase
      Short name=P5CS
Including the following 2 domains:
    1- Recommended name:
            Glutamate 5-kinase
                Short name=GK
              EC=2.7.2.11
        Alternative name(s):
            Gamma-glutamyl kinase
    2- Recommended name:
            Gamma-glutamyl phosphate reductase
                Short name=GPR
              EC=1.2.1.41
        Alternative name(s):
            Glutamate-5-semialdehyde dehydrogenase
            Glutamyl-gamma-semialdehyde dehydrogenase

Gene names

Name:	P5CS
Ordered Locus Names:	Os05g0455500, LOC_Os05g38150
ORF Names:	OJ1651_D06.9

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.
Enzyme regulation	Feedback regulated by proline.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Tissue specificity	Expressed at high levels in leaves and is inducible in roots subjected to salt stress.
Sequence similarities	In the N-terminal section; belongs to the glutamate 5-kinase family. In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: EC glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Delta-1-pyrroline-5-carboxylate synthetase		PRO_0000109777
Regions
	Region	1 – 296	296	Glutamate 5-kinase
	Region	297 – 716	420	Gamma-glutamyl phosphate reductase
Experimental info
	Sequence conflict	173	1	F → S in AAS89034. Ref.2
	Sequence conflict	488	1	I → T in BAA19916. Ref.1
	Sequence conflict	500	1	K → E in AAS89034. Ref.2
	Sequence conflict	530	1	H → L in BAA19916. Ref.1
	Sequence conflict	537	1	I → T in BAA19916. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04226-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: F8D3C7D9449CFA64	FASTA	716	77,745
	10 20 30 40 50 60 MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS LGYEVILVTS 70 80 90 100 110 120 GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ SGLMALYDML FNQLDVSSSQ 130 140 150 160 170 180 LLVTDSDFEN PKFREQLTET VESLLDLKVI PIFNENDAIS TRKAPYEDSS GIFWDNDSLA 190 200 210 220 230 240 GLLALELKAD LLILLSDVDG LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT 250 260 270 280 290 300 AKVKAAVLAS NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV 310 320 330 340 350 360 AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG YEKPLVARLT 370 380 390 400 410 420 IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK TSCPLGVLLI VFESRPDALV 430 440 450 460 470 480 QIASLAIRSG NGLLLKGGKE AIRSNTILHK VITDAIPRNV GEKLIGLVTT RDEIADLLKL 490 500 510 520 530 540 DDVIDLVIPR GSNKLVSQIK ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP 550 560 570 580 590 600 AACNAMETLL VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM 610 620 630 640 650 660 ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH NASTRFSDGA 670 680 690 700 710 RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV NGDKDVVYTH KSLPLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L." Igarashi Y., Yoshiba Y., Sanada Y., Yamaguchi-Shinozaki K., Wada K., Shinozaki K. Plant Mol. Biol. 33:857-865(1997) [PubMed: 9106509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Akibare.
[2]	"Nucleotide sequence of delta-1-pyrroline-5-carboxylate synthetase." Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Tainung 71.
[3]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D49714 mRNA. Translation: BAA19916.1. AY574031 mRNA. Translation: AAS89034.1. AC111016 Genomic DNA. Translation: AAU90213.1.
PIR	T03695.
RefSeq	NP_001055723.1.
UniGene	Os.12281
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
GeneID	4338979.
KEGG	osa:4338979.
Organism-specific databases
Gramene	O04226.
Family and domain databases
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR001048. Asp/Glu/Uridylate_kinase. IPR000965. Gglut_pp_reduct. IPR001057. Glu_5kinase. IPR019797. Glutamate_5-kinase_CS. IPR005766. P5_carboxy_syn. IPR005715. ProB. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF036429. P5C_syn. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR01092. P5CS. 1 hit. TIGR00407. proA. 1 hit. TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CS_ORYSJ

Accession

Primary (citable) accession number: O04226
Secondary accession number(s): Q60EM4, Q6PW76

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)