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O04226

- P5CS_ORYSJ

UniProt

O04226 - P5CS_ORYSJ

Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene

P5CS

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (30 May 2006)
      Previous versions | rss
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    Functioni

    P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
    L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

    Enzyme regulationi

    Feedback regulated by proline.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei60 – 601SubstrateBy similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Binding sitei176 – 1761Substrate; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi196 – 1972ATPBy similarity
    Nucleotide bindingi236 – 2427ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-EC
    3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. embryo development ending in seed dormancy Source: EnsemblPlants/Gramene
    2. L-proline biosynthetic process Source: UniProtKB-UniPathway
    3. pollen development Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Kinase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00098; UER00359.
    UPA00098; UER00360.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-1-pyrroline-5-carboxylate synthase
    Short name:
    P5CS
    Including the following 2 domains:
    Glutamate 5-kinase (EC:2.7.2.11)
    Short name:
    GK
    Alternative name(s):
    Gamma-glutamyl kinase
    Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
    Short name:
    GPR
    Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
    Gene namesi
    Name:P5CS
    Ordered Locus Names:Os05g0455500, LOC_Os05g38150
    ORF Names:OJ1651_D06.9
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 5

    Organism-specific databases

    GrameneiO04226.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: EnsemblPlants/Gramene
    2. cytosol Source: EnsemblPlants/Gramene
    3. plasmodesma Source: EnsemblPlants/Gramene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 716716Delta-1-pyrroline-5-carboxylate synthasePRO_0000109777Add
    BLAST

    Proteomic databases

    PRIDEiO04226.

    Expressioni

    Tissue specificityi

    Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

    Interactioni

    Protein-protein interaction databases

    STRINGi39946.BGIOSIBCE019273.

    Structurei

    3D structure databases

    ProteinModelPortaliO04226.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 296296Glutamate 5-kinaseAdd
    BLAST
    Regioni297 – 716420Gamma-glutamyl phosphate reductaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
    In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0014.
    KOiK12657.
    OMAiLLPWVQS.

    Family and domain databases

    Gene3Di3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPiMF_00412. ProA.
    MF_00456. ProB.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036429. P5C_syn. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O04226-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS    50
    LGYEVILVTS GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ 100
    SGLMALYDML FNQLDVSSSQ LLVTDSDFEN PKFREQLTET VESLLDLKVI 150
    PIFNENDAIS TRKAPYEDSS GIFWDNDSLA GLLALELKAD LLILLSDVDG 200
    LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT AKVKAAVLAS 250
    NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV 300
    AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG 350
    YEKPLVARLT IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK 400
    TSCPLGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE AIRSNTILHK 450
    VITDAIPRNV GEKLIGLVTT RDEIADLLKL DDVIDLVIPR GSNKLVSQIK 500
    ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP AACNAMETLL 550
    VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM 600
    ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH 650
    NASTRFSDGA RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV 700
    NGDKDVVYTH KSLPLQ 716
    Length:716
    Mass (Da):77,745
    Last modified:May 30, 2006 - v2
    Checksum:iF8D3C7D9449CFA64
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti173 – 1731F → S in AAS89034. 1 PublicationCurated
    Sequence conflicti488 – 4881I → T in BAA19916. (PubMed:9106509)Curated
    Sequence conflicti500 – 5001K → E in AAS89034. 1 PublicationCurated
    Sequence conflicti530 – 5301H → L in BAA19916. (PubMed:9106509)Curated
    Sequence conflicti537 – 5371I → T in BAA19916. (PubMed:9106509)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49714 mRNA. Translation: BAA19916.1.
    AY574031 mRNA. Translation: AAS89034.1.
    AC111016 Genomic DNA. Translation: AAU90213.1.
    PIRiT03695.
    RefSeqiNP_001055723.1. NM_001062258.1.
    UniGeneiOs.12281.

    Genome annotation databases

    EnsemblPlantsiOS05T0455500-02; OS05T0455500-02; OS05G0455500.
    GeneIDi4338979.
    KEGGiosa:4338979.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49714 mRNA. Translation: BAA19916.1 .
    AY574031 mRNA. Translation: AAS89034.1 .
    AC111016 Genomic DNA. Translation: AAU90213.1 .
    PIRi T03695.
    RefSeqi NP_001055723.1. NM_001062258.1.
    UniGenei Os.12281.

    3D structure databases

    ProteinModelPortali O04226.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39946.BGIOSIBCE019273.

    Proteomic databases

    PRIDEi O04226.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS05T0455500-02 ; OS05T0455500-02 ; OS05G0455500 .
    GeneIDi 4338979.
    KEGGi osa:4338979.

    Organism-specific databases

    Gramenei O04226.

    Phylogenomic databases

    eggNOGi COG0014.
    KOi K12657.
    OMAi LLPWVQS.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    UPA00098 ; UER00360 .

    Family and domain databases

    Gene3Di 3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPi MF_00412. ProA.
    MF_00456. ProB.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036429. P5C_syn. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L."
      Igarashi Y., Yoshiba Y., Sanada Y., Yamaguchi-Shinozaki K., Wada K., Shinozaki K.
      Plant Mol. Biol. 33:857-865(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Akibare.
    2. "Nucleotide sequence of delta-1-pyrroline-5-carboxylate synthetase."
      Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.
      Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Tainung 71.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiP5CS_ORYSJ
    AccessioniPrimary (citable) accession number: O04226
    Secondary accession number(s): Q60EM4, Q6PW76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3