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Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene

P5CS

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulationi

Feedback regulated by proline.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Binding sitei176 – 1761Substrate; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi196 – 1972ATPBy similarity
Nucleotide bindingi236 – 2427ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-EC
  3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_250903. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:P5CS
Ordered Locus Names:Os05g0455500, LOC_Os05g38150
ORF Names:OJ1651_D06.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 5

Organism-specific databases

GrameneiO04226.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Delta-1-pyrroline-5-carboxylate synthasePRO_0000109777Add
BLAST

Proteomic databases

PRIDEiO04226.

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Gene expression databases

ExpressionAtlasiO04226. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39946.BGIOSIBCE019273.

Structurei

3D structure databases

ProteinModelPortaliO04226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 296296Glutamate 5-kinaseAdd
BLAST
Regioni297 – 716420Gamma-glutamyl phosphate reductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

Phylogenomic databases

eggNOGiCOG0014.
InParanoidiO04226.
KOiK12657.
OMAiLLPWVQS.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS
60 70 80 90 100
LGYEVILVTS GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ
110 120 130 140 150
SGLMALYDML FNQLDVSSSQ LLVTDSDFEN PKFREQLTET VESLLDLKVI
160 170 180 190 200
PIFNENDAIS TRKAPYEDSS GIFWDNDSLA GLLALELKAD LLILLSDVDG
210 220 230 240 250
LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT AKVKAAVLAS
260 270 280 290 300
NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV
310 320 330 340 350
AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG
360 370 380 390 400
YEKPLVARLT IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK
410 420 430 440 450
TSCPLGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE AIRSNTILHK
460 470 480 490 500
VITDAIPRNV GEKLIGLVTT RDEIADLLKL DDVIDLVIPR GSNKLVSQIK
510 520 530 540 550
ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP AACNAMETLL
560 570 580 590 600
VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM
610 620 630 640 650
ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH
660 670 680 690 700
NASTRFSDGA RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV
710
NGDKDVVYTH KSLPLQ
Length:716
Mass (Da):77,745
Last modified:May 30, 2006 - v2
Checksum:iF8D3C7D9449CFA64
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731F → S in AAS89034 (Ref. 2) Curated
Sequence conflicti488 – 4881I → T in BAA19916 (PubMed:9106509).Curated
Sequence conflicti500 – 5001K → E in AAS89034 (Ref. 2) Curated
Sequence conflicti530 – 5301H → L in BAA19916 (PubMed:9106509).Curated
Sequence conflicti537 – 5371I → T in BAA19916 (PubMed:9106509).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49714 mRNA. Translation: BAA19916.1.
AY574031 mRNA. Translation: AAS89034.1.
AC111016 Genomic DNA. Translation: AAU90213.1.
PIRiT03695.
RefSeqiNP_001055723.1. NM_001062258.1.
UniGeneiOs.12281.

Genome annotation databases

EnsemblPlantsiOS05T0455500-02; OS05T0455500-02; OS05G0455500.
GeneIDi4338979.
KEGGiosa:4338979.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49714 mRNA. Translation: BAA19916.1.
AY574031 mRNA. Translation: AAS89034.1.
AC111016 Genomic DNA. Translation: AAU90213.1.
PIRiT03695.
RefSeqiNP_001055723.1. NM_001062258.1.
UniGeneiOs.12281.

3D structure databases

ProteinModelPortaliO04226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39946.BGIOSIBCE019273.

Proteomic databases

PRIDEiO04226.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS05T0455500-02; OS05T0455500-02; OS05G0455500.
GeneIDi4338979.
KEGGiosa:4338979.

Organism-specific databases

GrameneiO04226.

Phylogenomic databases

eggNOGiCOG0014.
InParanoidiO04226.
KOiK12657.
OMAiLLPWVQS.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.
ReactomeiREACT_250903. Amino acid synthesis and interconversion (transamination).

Gene expression databases

ExpressionAtlasiO04226. baseline.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L."
    Igarashi Y., Yoshiba Y., Sanada Y., Yamaguchi-Shinozaki K., Wada K., Shinozaki K.
    Plant Mol. Biol. 33:857-865(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Akibare.
  2. "Nucleotide sequence of delta-1-pyrroline-5-carboxylate synthetase."
    Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Tainung 71.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiP5CS_ORYSJ
AccessioniPrimary (citable) accession number: O04226
Secondary accession number(s): Q60EM4, Q6PW76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2006
Last modified: January 7, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.