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O04226

- P5CS_ORYSJ

UniProt

O04226 - P5CS_ORYSJ

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Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene
P5CS, Os05g0455500, LOC_Os05g38150, OJ1651_D06.9
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulationi

Feedback regulated by proline.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601Substrate By similarity
Binding sitei157 – 1571Substrate By similarity
Binding sitei176 – 1761Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi196 – 1972ATP By similarity
Nucleotide bindingi236 – 2427ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-EC
  3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. embryo development ending in seed dormancy Source: EnsemblPlants/Gramene
  2. L-proline biosynthetic process Source: UniProtKB-UniPathway
  3. pollen development Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:P5CS
Ordered Locus Names:Os05g0455500, LOC_Os05g38150
ORF Names:OJ1651_D06.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 5

Organism-specific databases

GrameneiO04226.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: EnsemblPlants/Gramene
  2. cytosol Source: EnsemblPlants/Gramene
  3. plasmodesma Source: EnsemblPlants/Gramene
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Delta-1-pyrroline-5-carboxylate synthasePRO_0000109777Add
BLAST

Proteomic databases

PRIDEiO04226.

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Interactioni

Protein-protein interaction databases

STRINGi39946.BGIOSIBCE019273.

Structurei

3D structure databases

ProteinModelPortaliO04226.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 296296Glutamate 5-kinaseAdd
BLAST
Regioni297 – 716420Gamma-glutamyl phosphate reductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Phylogenomic databases

eggNOGiCOG0014.
KOiK12657.
OMAiLLPWVQS.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04226-1 [UniParc]FASTAAdd to Basket

« Hide

MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS    50
LGYEVILVTS GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ 100
SGLMALYDML FNQLDVSSSQ LLVTDSDFEN PKFREQLTET VESLLDLKVI 150
PIFNENDAIS TRKAPYEDSS GIFWDNDSLA GLLALELKAD LLILLSDVDG 200
LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT AKVKAAVLAS 250
NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV 300
AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG 350
YEKPLVARLT IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK 400
TSCPLGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE AIRSNTILHK 450
VITDAIPRNV GEKLIGLVTT RDEIADLLKL DDVIDLVIPR GSNKLVSQIK 500
ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP AACNAMETLL 550
VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM 600
ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH 650
NASTRFSDGA RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV 700
NGDKDVVYTH KSLPLQ 716
Length:716
Mass (Da):77,745
Last modified:May 30, 2006 - v2
Checksum:iF8D3C7D9449CFA64
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731F → S in AAS89034. 1 Publication
Sequence conflicti488 – 4881I → T in BAA19916. 1 Publication
Sequence conflicti500 – 5001K → E in AAS89034. 1 Publication
Sequence conflicti530 – 5301H → L in BAA19916. 1 Publication
Sequence conflicti537 – 5371I → T in BAA19916. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49714 mRNA. Translation: BAA19916.1.
AY574031 mRNA. Translation: AAS89034.1.
AC111016 Genomic DNA. Translation: AAU90213.1.
PIRiT03695.
RefSeqiNP_001055723.1. NM_001062258.1.
UniGeneiOs.12281.

Genome annotation databases

EnsemblPlantsiOS05T0455500-02; OS05T0455500-02; OS05G0455500.
GeneIDi4338979.
KEGGiosa:4338979.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49714 mRNA. Translation: BAA19916.1 .
AY574031 mRNA. Translation: AAS89034.1 .
AC111016 Genomic DNA. Translation: AAU90213.1 .
PIRi T03695.
RefSeqi NP_001055723.1. NM_001062258.1.
UniGenei Os.12281.

3D structure databases

ProteinModelPortali O04226.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39946.BGIOSIBCE019273.

Proteomic databases

PRIDEi O04226.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS05T0455500-02 ; OS05T0455500-02 ; OS05G0455500 .
GeneIDi 4338979.
KEGGi osa:4338979.

Organism-specific databases

Gramenei O04226.

Phylogenomic databases

eggNOGi COG0014.
KOi K12657.
OMAi LLPWVQS.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
UPA00098 ; UER00360 .

Family and domain databases

Gene3Di 3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPi MF_00412. ProA.
MF_00456. ProB.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view ]
PIRSFi PIRSF036429. P5C_syn. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L."
    Igarashi Y., Yoshiba Y., Sanada Y., Yamaguchi-Shinozaki K., Wada K., Shinozaki K.
    Plant Mol. Biol. 33:857-865(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Akibare.
  2. "Nucleotide sequence of delta-1-pyrroline-5-carboxylate synthetase."
    Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Tainung 71.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiP5CS_ORYSJ
AccessioniPrimary (citable) accession number: O04226
Secondary accession number(s): Q60EM4, Q6PW76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2006
Last modified: September 3, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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