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O04226 (P5CS_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-1-pyrroline-5-carboxylate synthase

Short name=P5CS

Including the following 2 domains:

  1. Glutamate 5-kinase
    Short name=GK
    EC=2.7.2.11
    Alternative name(s):
    Gamma-glutamyl kinase
  2. Gamma-glutamyl phosphate reductase
    Short name=GPR
    EC=1.2.1.41
    Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
Gene names
Name:P5CS
Ordered Locus Names:Os05g0455500, LOC_Os05g38150
ORF Names:OJ1651_D06.9
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length716 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulation

Feedback regulated by proline.

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.

Tissue specificity

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Sequence similarities

In the N-terminal section; belongs to the glutamate 5-kinase family.

In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 716716Delta-1-pyrroline-5-carboxylate synthase
PRO_0000109777

Regions

Nucleotide binding196 – 1972ATP By similarity
Nucleotide binding236 – 2427ATP By similarity
Region1 – 296296Glutamate 5-kinase
Region297 – 716420Gamma-glutamyl phosphate reductase

Sites

Binding site601Substrate By similarity
Binding site1571Substrate By similarity
Binding site1761Substrate; via amide nitrogen By similarity

Experimental info

Sequence conflict1731F → S in AAS89034. Ref.2
Sequence conflict4881I → T in BAA19916. Ref.1
Sequence conflict5001K → E in AAS89034. Ref.2
Sequence conflict5301H → L in BAA19916. Ref.1
Sequence conflict5371I → T in BAA19916. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O04226 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: F8D3C7D9449CFA64

FASTA71677,745
        10         20         30         40         50         60 
MASVDPSRSF VRDVKRVIIK VGTAVVSRQD GRLALGRVGA LCEQVKELNS LGYEVILVTS 

        70         80         90        100        110        120 
GAVGVGRQRL RYRKLVNSSF ADLQKPQMEL DGKACAAVGQ SGLMALYDML FNQLDVSSSQ 

       130        140        150        160        170        180 
LLVTDSDFEN PKFREQLTET VESLLDLKVI PIFNENDAIS TRKAPYEDSS GIFWDNDSLA 

       190        200        210        220        230        240 
GLLALELKAD LLILLSDVDG LYSGPPSEPS SKIIHTYIKE KHQQEITFGD KSRVGRGGMT 

       250        260        270        280        290        300 
AKVKAAVLAS NSGTPVVITS GFENRSILKV LHGEKIGTLF HKNANLWESS KDVSTREMAV 

       310        320        330        340        350        360 
AARDCSRHLQ NLSSEERKKI LLDVADALEA NEDLIRSENE ADVAAAQVAG YEKPLVARLT 

       370        380        390        400        410        420 
IKPGKIASLA KSIRTLANME DPINQILKKT EVADDLVLEK TSCPLGVLLI VFESRPDALV 

       430        440        450        460        470        480 
QIASLAIRSG NGLLLKGGKE AIRSNTILHK VITDAIPRNV GEKLIGLVTT RDEIADLLKL 

       490        500        510        520        530        540 
DDVIDLVIPR GSNKLVSQIK ASTKIPVLGH ADGICHVYID KSADMDMAKH IVMDAKIDYP 

       550        560        570        580        590        600 
AACNAMETLL VHKDLMKSPG LDDILVALKT EGVNIYGGPI AHKALGFPKA VSFHHEYSSM 

       610        620        630        640        650        660 
ACTVEFVDDV QSAIDHIHRY GSAHTDCIVT TDDKVAETFL RRVDSAAVFH NASTRFSDGA 

       670        680        690        700        710 
RFGLGAEVGI STGRIHARGP VGVEGLLTTR WILRGRGQVV NGDKDVVYTH KSLPLQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the gene for delta1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L."
Igarashi Y., Yoshiba Y., Sanada Y., Yamaguchi-Shinozaki K., Wada K., Shinozaki K.
Plant Mol. Biol. 33:857-865(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Akibare.
[2]"Nucleotide sequence of delta-1-pyrroline-5-carboxylate synthetase."
Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Tainung 71.
[3]"A fine physical map of the rice chromosome 5."
Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.
Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D49714 mRNA. Translation: BAA19916.1.
AY574031 mRNA. Translation: AAS89034.1.
AC111016 Genomic DNA. Translation: AAU90213.1.
PIRT03695.
RefSeqNP_001055723.1. NM_001062258.1.
UniGeneOs.12281.

3D structure databases

ProteinModelPortalO04226.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39946.BGIOSIBCE019273.

Proteomic databases

PRIDEO04226.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS05T0455500-02; OS05T0455500-02; OS05G0455500.
GeneID4338979.
KEGGosa:4338979.

Organism-specific databases

GrameneO04226.

Phylogenomic databases

eggNOGCOG0014.
KOK12657.
OMALLPWVQS.
ProtClustDBPLN02418.

Enzyme and pathway databases

UniPathwayUPA00098; UER00359.
UPA00098; UER00360.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFPIRSF036429. P5C_syn. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP5CS_ORYSJ
AccessionPrimary (citable) accession number: O04226
Secondary accession number(s): Q60EM4, Q6PW76
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2006
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways