Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic translation initiation factor 3 subunit F

Gene

TIF3F1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (Potential). Involved in cell growth and differentiation, especially during embryogenesis and male gametophyte germination (PubMed:20444226). Regulates sensitivity to sugars (e.g. sucrose) (PubMed:20444226).UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • pollen germination Source: TAIR
  • response to sucrose Source: UniProtKB
  • translational initiation Source: GO_Central

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-ATH-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-ATH-72689 Formation of a pool of free 40S subunits
R-ATH-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-ATH-72702 Ribosomal scanning and start codon recognition

Protein family/group databases

MEROPSiM67.974

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit FUniRule annotation
Short name:
eIF3fUniRule annotation
Alternative name(s):
eIF-3-epsilonUniRule annotation
eIF3 p32 subunit
Gene namesi
Name:TIF3F1
Ordered Locus Names:At2g39990
ORF Names:T29M21.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G39990
TAIRilocus:2061206 AT2G39990

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Male gametophyte-defective. Disrupted pollen germination and embryo development. Hypersensitivity to exogenous sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002139662 – 293Eukaryotic translation initiation factor 3 subunit FAdd BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO04202
PRIDEiO04202

PTM databases

iPTMnetiO04202

Expressioni

Tissue specificityi

Expressed in inflorescences, leaves, stems, siliques, roots and seedlings. Accumulates at highly levels in pollen grains, developing embryos and root tips.1 Publication

Gene expression databases

ExpressionAtlasiO04202 baseline and differential
GenevisibleiO04202 AT

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Binds to TIF3E1 and TIF3H1 (PubMed:20444226).UniRule annotation1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi3925, 1 interactor
STRINGi3702.AT2G39990.1

Structurei

3D structure databases

ProteinModelPortaliO04202
SMRiO04202
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 159MPNPROSITE-ProRule annotationAdd BLAST132

Sequence similaritiesi

Belongs to the eIF-3 subunit F family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2975 Eukaryota
COG1310 LUCA
HOGENOMiHOG000241154
InParanoidiO04202
KOiK03249
OMAiAVDMEYH
OrthoDBiEOG09360JFH
PhylomeDBiO04202

Family and domain databases

CDDicd08064 MPN_eIF3f, 1 hit
HAMAPiMF_03005 eIF3f, 1 hit
InterProiView protein in InterPro
IPR027531 eIF3f
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
IPR024969 Rpn11/EIF3F_C
PANTHERiPTHR10540:SF6 PTHR10540:SF6, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
PF13012 MitMem_reg, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATSEHTIL QFVSPSSTAS ATTSVLTARI HPLVIFNVCD CFVRRPDSAE
60 70 80 90 100
RVIGTLLGSI LPDGTVDIRN SYAVPHNESS DQVAVDIDYH HNMLASHLKV
110 120 130 140 150
NSKETIVGWY STGAGVNGGS SLIHDFYARE VPNPIHLTVD TGFTNGEGTI
160 170 180 190 200
KAFVSSNLSL GDRQLVAHFQ EIPVDLRMVD AERVGFDVLK ATSVDKLPND
210 220 230 240 250
LEGMELTMER LLTLINDVYK YVDSVVGGQI APDNNIGRFI ADAVASLPKL
260 270 280 290
PPQVFDNLVN DSLQDQLLLL YLSSITRTQL SLAEKLNTAA QML
Length:293
Mass (Da):31,862
Last modified:July 1, 1997 - v1
Checksum:i4742CF7E8F6F47C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261D → N in AAL32634 (PubMed:14593172).Curated1
Sequence conflicti261D → N in AAM13367 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54561 mRNA Translation: AAD03463.1
AF002109 Genomic DNA Translation: AAB95284.1
CP002685 Genomic DNA Translation: AEC09760.1
AY045824 mRNA Translation: AAK76498.1
AY062556 mRNA Translation: AAL32634.1
AY091363 mRNA Translation: AAM14302.1
AY093368 mRNA Translation: AAM13367.1
PIRiH84823
RefSeqiNP_181528.1, NM_129557.4
UniGeneiAt.10968

Genome annotation databases

EnsemblPlantsiAT2G39990.1; AT2G39990.1; AT2G39990
GeneIDi818587
GrameneiAT2G39990.1; AT2G39990.1; AT2G39990
KEGGiath:AT2G39990

Similar proteinsi

Entry informationi

Entry nameiEIF3F_ARATH
AccessioniPrimary (citable) accession number: O04202
Secondary accession number(s): Q8W4H4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 1, 1997
Last modified: April 25, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health