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Protein

Eukaryotic translation initiation factor 3 subunit F

Gene

TIF3F1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. Involved in cell growth and differentiation, especially during embryogenesis and male gametophyte germination. Regulates sensitivity to sugars (e.g. sucrose) (PubMed:20444226).UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • formation of translation preinitiation complex Source: UniProtKB-HAMAP
  • pollen germination Source: TAIR
  • regulation of translational initiation Source: UniProtKB-HAMAP
  • response to sucrose Source: UniProtKB
  • translational initiation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-72689. Formation of a pool of free 40S subunits.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.

Protein family/group databases

MEROPSiM67.974.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit FUniRule annotation
Short name:
eIF3fUniRule annotation
Alternative name(s):
eIF-3-epsilonUniRule annotation
eIF3 p32 subunit
Gene namesi
Name:TIF3F1
Ordered Locus Names:At2g39990
ORF Names:T29M21.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G39990.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
  • eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
  • eukaryotic translation initiation factor 3 complex, eIF3m Source: GO_Central
  • membrane Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Male gametophyte-defective. Disrupted pollen germination and embryo development. Hypersensitivity to exogenous sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 293292Eukaryotic translation initiation factor 3 subunit FPRO_0000213966Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO04202.
PRIDEiO04202.

Expressioni

Tissue specificityi

Expressed in inflorescences, leaves, stems, siliques, roots and seedlings. Accumulates at highly levels in pollen grains, developing embryos and root tips.1 Publication

Gene expression databases

GenevisibleiO04202. AT.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Binds to TIF3E1 and TIF3H1 (PubMed:20444226).UniRule annotation1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi3925. 1 interaction.
STRINGi3702.AT2G39990.1.

Structurei

3D structure databases

ProteinModelPortaliO04202.
SMRiO04202. Positions 29-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 133111MPNUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit F family.UniRule annotation
Contains 1 MPN (JAB/Mov34) domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG2975. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000241154.
InParanoidiO04202.
KOiK03249.
OMAiDAYERRN.
PhylomeDBiO04202.

Family and domain databases

HAMAPiMF_03005. eIF3f.
InterProiIPR027531. eIF3f.
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATSEHTIL QFVSPSSTAS ATTSVLTARI HPLVIFNVCD CFVRRPDSAE
60 70 80 90 100
RVIGTLLGSI LPDGTVDIRN SYAVPHNESS DQVAVDIDYH HNMLASHLKV
110 120 130 140 150
NSKETIVGWY STGAGVNGGS SLIHDFYARE VPNPIHLTVD TGFTNGEGTI
160 170 180 190 200
KAFVSSNLSL GDRQLVAHFQ EIPVDLRMVD AERVGFDVLK ATSVDKLPND
210 220 230 240 250
LEGMELTMER LLTLINDVYK YVDSVVGGQI APDNNIGRFI ADAVASLPKL
260 270 280 290
PPQVFDNLVN DSLQDQLLLL YLSSITRTQL SLAEKLNTAA QML
Length:293
Mass (Da):31,862
Last modified:July 1, 1997 - v1
Checksum:i4742CF7E8F6F47C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611D → N in AAL32634 (PubMed:14593172).Curated
Sequence conflicti261 – 2611D → N in AAM13367 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54561 mRNA. Translation: AAD03463.1.
AF002109 Genomic DNA. Translation: AAB95284.1.
CP002685 Genomic DNA. Translation: AEC09760.1.
AY045824 mRNA. Translation: AAK76498.1.
AY062556 mRNA. Translation: AAL32634.1.
AY091363 mRNA. Translation: AAM14302.1.
AY093368 mRNA. Translation: AAM13367.1.
PIRiH84823.
RefSeqiNP_181528.1. NM_129557.3.
UniGeneiAt.10968.

Genome annotation databases

EnsemblPlantsiAT2G39990.1; AT2G39990.1; AT2G39990.
GeneIDi818587.
GrameneiAT2G39990.1; AT2G39990.1; AT2G39990.
KEGGiath:AT2G39990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54561 mRNA. Translation: AAD03463.1.
AF002109 Genomic DNA. Translation: AAB95284.1.
CP002685 Genomic DNA. Translation: AEC09760.1.
AY045824 mRNA. Translation: AAK76498.1.
AY062556 mRNA. Translation: AAL32634.1.
AY091363 mRNA. Translation: AAM14302.1.
AY093368 mRNA. Translation: AAM13367.1.
PIRiH84823.
RefSeqiNP_181528.1. NM_129557.3.
UniGeneiAt.10968.

3D structure databases

ProteinModelPortaliO04202.
SMRiO04202. Positions 29-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3925. 1 interaction.
STRINGi3702.AT2G39990.1.

Protein family/group databases

MEROPSiM67.974.

Proteomic databases

PaxDbiO04202.
PRIDEiO04202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G39990.1; AT2G39990.1; AT2G39990.
GeneIDi818587.
GrameneiAT2G39990.1; AT2G39990.1; AT2G39990.
KEGGiath:AT2G39990.

Organism-specific databases

TAIRiAT2G39990.

Phylogenomic databases

eggNOGiKOG2975. Eukaryota.
COG1310. LUCA.
HOGENOMiHOG000241154.
InParanoidiO04202.
KOiK03249.
OMAiDAYERRN.
PhylomeDBiO04202.

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-72689. Formation of a pool of free 40S subunits.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

PROiO04202.

Gene expression databases

GenevisibleiO04202. AT.

Family and domain databases

HAMAPiMF_03005. eIF3f.
InterProiIPR027531. eIF3f.
IPR000555. JAMM/MPN+_dom.
IPR024969. Rpn11/EIF3F_C.
[Graphical view]
PfamiPF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
[Graphical view]
SMARTiSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly."
    Asano K., Vornlocher H.-P., Richter-Cook N.J., Merrick W.C., Hinnebusch A.G., Hershey J.W.B.
    J. Biol. Chem. 272:27042-27052(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The Arabidopsis eukaryotic translation initiation factor 3, subunit F (AteIF3f), is required for pollen germination and embryogenesis."
    Xia C., Wang Y.-J., Li W.-Q., Chen Y.-R., Deng Y., Zhang X.-Q., Chen L.-Q., Ye D.
    Plant J. 63:189-202(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INTERACTION WITH TIF3E1 AND TIF3H1, SUBCELLULAR LOCATION.
    Strain: cv. Landsberg erecta and cv. Wassilewskija.
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEIF3F_ARATH
AccessioniPrimary (citable) accession number: O04202
Secondary accession number(s): Q8W4H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 1, 1997
Last modified: February 17, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.