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O04197 (COI1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Coronatine-insensitive protein 1
Alternative name(s):
COI-1
F-box/LRR-repeat protein 2
Short name=AtCOI1
Short name=AtFBL2
Gene names
Name:COI1
Synonyms:FBL2
Ordered Locus Names:At2g39940
ORF Names:T28M21.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Seems to not be required for meiosis. Required for the regulation of some genes induced by wounding, but not for all. Component of SCF(COI1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including the ribulose bisphosphate carboxylase small chain 1B RBCS-1B and the histone deacetylase HDA6). These SCF complexes play crucial roles in regulating response to jasmonate, and their interactions with the COP9 signalosome (CSN) appear to be important for their activity. Interacts with TIFY10A and inositol pentakisphosphate to form a high-affinity jasmonates coreceptor. Involved in the regulation of plant gene expression during plant-pathogen interactions with Pseudomonas syringae and Alternaria brassicicola. Ref.1 Ref.6 Ref.7 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.16

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Component of SCF(COI1) E3 ubiquitin ligase complexes at least composed of ASK1 or ASK2, CUL1, RBX1A or RBX1B and COI1. Interacts with ASK1 and ASK2, but separately, Binds also to ASK11 and ASK12. Interacts with RBCS-1B and HDA6. SCF complexes interact with the COP9 signalosome (CSN). Interacts with TIFY10A. Ref.10 Ref.11 Ref.13 Ref.15 Ref.17

Domain

The F-box domain is essential for the formation of SFC(COI1) complexes. Ref.12

The Leu-rich domain is involved in the interactions with RBCS-1B and RPD3B. Ref.12

Disruption phenotype

Mutants coi1-1 to coi1-14 are male sterile, insensitive to MeJA and coronatine, and exhibit enhanced resistance to Pseudomonas syringae atropurpurea (coronatine producing strain). Mutant coi1-16 has reduced sensitivity to jasmonate, but is male fertile when grown below 22 degrees Celsius and male sterile otherwise. Ref.1

Sequence similarities

Contains 1 F-box domain.

Contains 18 LRR (leucine-rich) repeats.

Ontologies

Keywords
   Biological processJasmonic acid signaling pathway
Plant defense
Ubl conjugation pathway
   DomainLeucine-rich repeat
Repeat
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Inferred from mutant phenotype PubMed 17637675. Source: TAIR

defense response

Traceable author statement Ref.1. Source: TAIR

defense response to bacterium

Inferred from mutant phenotype PubMed 16959575. Source: TAIR

defense response to fungus

Inferred from mutant phenotype PubMed 17513501. Source: TAIR

jasmonic acid and ethylene-dependent systemic resistance

Traceable author statement PubMed 11418339. Source: TAIR

jasmonic acid mediated signaling pathway

Inferred from genetic interaction PubMed 15860010. Source: TAIR

negative regulation of defense response

Inferred from mutant phenotype PubMed 16732289. Source: TAIR

protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-UniPathway

regulation of flower development

Inferred from mutant phenotype PubMed 20435902. Source: TAIR

response to far red light

Inferred from mutant phenotype PubMed 20435902. Source: TAIR

response to insect

Inferred from mutant phenotype PubMed 15923339. Source: TAIR

response to jasmonic acid

Inferred from mutant phenotype PubMed 16021335. Source: TAIR

response to wounding

Inferred from mutant phenotype PubMed 16021335. Source: TAIR

shade avoidance

Inferred from mutant phenotype PubMed 20435902. Source: TAIR

stomatal movement

Inferred from mutant phenotype PubMed 16959575. Source: TAIR

   Cellular_componentSCF ubiquitin ligase complex

Inferred from physical interaction PubMed 16021335. Source: TAIR

   Molecular_functionprotein binding

Inferred from physical interaction Ref.10Ref.11Ref.15PubMed 17637675PubMed 17637677PubMed 18547396PubMed 19309455PubMed 21798944. Source: IntAct

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 592592Coronatine-insensitive protein 1
PRO_0000119960

Regions

Domain16 – 5742F-box
Repeat58 – 8225LRR 1
Repeat83 – 10220LRR 2
Repeat103 – 12018LRR 3
Repeat121 – 15434LRR 4
Repeat155 – 18228LRR 5
Repeat183 – 21028LRR 6
Repeat211 – 23626LRR 7
Repeat237 – 26428LRR 8
Repeat265 – 28319LRR 8
Repeat284 – 30825LRR 10
Repeat309 – 33224LRR 11
Repeat333 – 36836LRR 12
Repeat369 – 39325LRR 13
Repeat394 – 42633LRR 14
Repeat427 – 45630LRR 15
Repeat457 – 47822LRR 16
Repeat479 – 50022LRR 17
Repeat501 – 52424LRR 18
Compositional bias102 – 517416Leu-rich

Sites

Binding site851Jasmonate
Binding site3481Jasmonate
Binding site3501Jasmonate
Binding site3861Jasmonate
Binding site4091Jasmonate
Binding site4961Jasmonate

Experimental info

Mutagenesis111L → A: No effects on interactions. Ref.11
Mutagenesis221E → A: Abrogates SFC(COI1) complexes formation, loss of response to jasmonate. Ref.10
Mutagenesis441W → A: Abrogates SFC(COI1) complexes formation and of interactions with RBCS-1B and RPD3B, loss of response to jasmonate. Ref.11
Mutagenesis851R → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis881M → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis891F → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis1211R → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis2451L → F in coi1-16; abrogates interactions with RBCS-1B and RPD3B (coi1-16). Ref.9 Ref.11
Mutagenesis3011L → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis3021Y → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis3261R → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis3481R → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis3511R → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis3861Y → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis4091R → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis4441Y → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis4691L → A: Loss of interaction with TIFY10A. Ref.17
Mutagenesis4961R → A: Loss of interaction with TIFY10A. Ref.17

Secondary structure

..................................................................................................... 592
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O04197 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 1DDCF04990144C06

FASTA59267,665
        10         20         30         40         50         60 
MEDPDIKRCK LSCVATVDDV IEQVMTYITD PKDRDSASLV CRRWFKIDSE TREHVTMALC 

        70         80         90        100        110        120 
YTATPDRLSR RFPNLRSLKL KGKPRAAMFN LIPENWGGYV TPWVTEISNN LRQLKSVHFR 

       130        140        150        160        170        180 
RMIVSDLDLD RLAKARADDL ETLKLDKCSG FTTDGLLSIV THCRKIKTLL MEESSFSEKD 

       190        200        210        220        230        240 
GKWLHELAQH NTSLEVLNFY MTEFAKISPK DLETIARNCR SLVSVKVGDF EILELVGFFK 

       250        260        270        280        290        300 
AAANLEEFCG GSLNEDIGMP EKYMNLVFPR KLCRLGLSYM GPNEMPILFP FAAQIRKLDL 

       310        320        330        340        350        360 
LYALLETEDH CTLIQKCPNL EVLETRNVIG DRGLEVLAQY CKQLKRLRIE RGADEQGMED 

       370        380        390        400        410        420 
EEGLVSQRGL IALAQGCQEL EYMAVYVSDI TNESLESIGT YLKNLCDFRL VLLDREERIT 

       430        440        450        460        470        480 
DLPLDNGVRS LLIGCKKLRR FAFYLRQGGL TDLGLSYIGQ YSPNVRWMLL GYVGESDEGL 

       490        500        510        520        530        540 
MEFSRGCPNL QKLEMRGCCF SERAIAAAVT KLPSLRYLWV QGYRASMTGQ DLMQMARPYW 

       550        560        570        580        590 
NIELIPSRRV PEVNQQGEIR EMEHPAHILA YYSLAGQRTD CPTTVRVLKE PI 

« Hide

References

« Hide 'large scale' references
[1]"COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility."
Xie D., Feys B.F., James S., Nieto-Rostro M., Turner J.G.
Science 280:1091-1094(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
Strain: cv. Columbia.
[2]"Genes encoding defense signaling proteins in plants show weaker signatures of selection than those encoding recognition proteins."
Caldwell K.S., Michelmore R.W.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Aa-0, cv. Ak-1, cv. Bay-0, cv. C24, cv. Columbia, cv. Cvi-0, cv. Di-0, cv. Ei-2, cv. Gu-0, cv. HOG, cv. Landsberg erecta, cv. Lz-0, cv. Nd-1, cv. Sha, cv. Sorbo, cv. Tsu-0, cv. Wassilewskija and cv. Wei-0.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen."
Feys B.J.F., Benedetti C.S., Penfold C.N., Turner J.G.
Plant Cell 6:751-759(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: cv. Columbia.
[7]"Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis."
Reymond P., Weber H., Damond M., Farmer E.E.
Plant Cell 12:707-720(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"F-box proteins in Arabidopsis."
Xiao W., Jang J.-C.
Trends Plant Sci. 5:454-457(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings."
Ellis C., Turner J.G.
Planta 215:549-556(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LEU-245.
[10]"The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis."
Xu L., Liu F., Lechner E., Genschik P., Crosby W.L., Ma H., Peng W., Huang D., Xie D.
Plant Cell 14:1919-1935(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF GLU-22.
[11]"COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis."
Devoto A., Nieto-Rostro M., Xie D., Ellis C., Harmston R., Patrick E., Davis J., Sherratt L., Coleman M., Turner J.G.
Plant J. 32:457-466(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF LEU-11; TRP-44 AND LEU-245.
[12]"Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression."
Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.
Plant Mol. Biol. 49:69-79(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN LEUCINE-RICH REPEATS.
[13]"The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses."
Feng S., Ma L., Wang X., Xie D., Dinesh-Kumar S.P., Wei N., Deng X.W.
Plant Cell 15:1083-1094(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[14]"Characterization of the early response of Arabidopsis to Alternaria brassicicola infection using expression profiling."
van Wees S.C.M., Chang H.-S., Zhu T., Glazebrook J.
Plant Physiol. 132:606-617(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[15]"Expression and interaction analysis of Arabidopsis Skp1-related genes."
Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K., Shimada H., Matsui M.
Plant Cell Physiol. 45:83-91(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK11 AND ASK12.
[16]"Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine."
He P., Chintamanani S., Chen Z., Zhu L., Kunkel B.N., Alfano J.R., Tang X., Zhou J.-M.
Plant J. 37:589-602(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor."
Sheard L.B., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds T.R., Kobayashi Y., Hsu F.F., Sharon M., Browse J., He S.Y., Rizo J., Howe G.A., Zheng N.
Nature 468:400-405(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS)1-592 IN COMPLEX WITH JASMONOYL-ISOLEUCINE AND CORONATINE, INTERACTION WITH SKP1A AND TIFY10A, MUTAGENESIS OF ARG-85; MET-88; PHE-89; ARG-121; LEU-301; TYR-302; ARG-326; ARG-348; ARG-351; TYR-386; ARG-409; TYR-444; LEU-469 AND ARG-496.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF036340 mRNA. Translation: AAC17498.1.
EF470606 Genomic DNA. Translation: ABR45936.1.
EF470607 Genomic DNA. Translation: ABR45937.1.
EF470608 Genomic DNA. Translation: ABR45938.1.
EF470609 Genomic DNA. Translation: ABR45939.1.
EF470610 Genomic DNA. Translation: ABR45940.1.
EF470611 Genomic DNA. Translation: ABR45941.1.
EF470612 Genomic DNA. Translation: ABR45942.1.
EF470613 Genomic DNA. Translation: ABR45943.1.
EF470614 Genomic DNA. Translation: ABR45944.1.
EF470615 Genomic DNA. Translation: ABR45945.1.
EF470616 Genomic DNA. Translation: ABR45946.1.
EF470617 Genomic DNA. Translation: ABR45947.1.
EF470619 Genomic DNA. Translation: ABR45949.1.
EF470620 Genomic DNA. Translation: ABR45950.1.
EF470621 Genomic DNA. Translation: ABR45951.1.
EF470622 Genomic DNA. Translation: ABR45952.1.
EF470623 Genomic DNA. Translation: ABR45953.1.
EF470624 Genomic DNA. Translation: ABR45954.1.
AF002109 Genomic DNA. Translation: AAB95279.1.
CP002685 Genomic DNA. Translation: AEC09753.1.
AY045625 mRNA. Translation: AAK73983.1.
AY133556 mRNA. Translation: AAM91386.1.
PIRT52139.
RefSeqNP_565919.1. NM_129552.3.
UniGeneAt.20831.
At.71018.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3OGKX-ray2.80B/D/F/H/J/L/N/P1-592[»]
3OGLX-ray3.18B/D/F/H/J/L/N/P1-592[»]
3OGMX-ray3.34B/D/F/H/J/L/N/P1-592[»]
ProteinModelPortalO04197.
SMRO04197. Positions 12-592.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid3919. 15 interactions.
DIPDIP-31324N.
IntActO04197. 13 interactions.

Proteomic databases

PaxDbO04197.
PRIDEO04197.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G39940.1; AT2G39940.1; AT2G39940.
GeneID818581.
KEGGath:AT2G39940.

Organism-specific databases

GeneFarm4758.
TAIRAT2G39940.

Phylogenomic databases

eggNOGNOG262527.
HOGENOMHOG000238474.
InParanoidO04197.
KOK13463.
OMARRFAFYV.
PhylomeDBO04197.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressO04197.
GenevestigatorO04197.

Family and domain databases

ProtoNetSearch...

Other

EvolutionaryTraceO04197.
PROO04197.

Entry information

Entry nameCOI1_ARATH
AccessionPrimary (citable) accession number: O04197
Secondary accession number(s): B2BD84
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names