O04197 (COI1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coronatine-insensitive protein 1

Alternative name(s):
COI-1
F-box/LRR-repeat protein 2
Short name=AtCOI1
Short name=AtFBL2

Gene names

Name:	COI1
Synonyms:	FBL2
Ordered Locus Names:	At2g39940
ORF Names:	T28M21.10

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Seems to not be required for meiosis. Required for the regulation of some genes induced by wounding, but not for all. Component of SCF(COI1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including the ribulose bisphosphate carboxylase small chain 1B RBCS-1B and the histone deacetylase HDA6). These SCF complexes play crucial roles in regulating response to jasmonate, and their interactions with the COP9 signalosome (CSN) appear to be important for their activity. Interacts with TIFY10A and inositol pentakisphosphate to form a high-affinity jasmonates coreceptor. Involved in the regulation of plant gene expression during plant-pathogen interactions with Pseudomonas syringae and Alternaria brassicicola. Ref.1 Ref.6 Ref.7 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.16
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of SCF(COI1) E3 ubiquitin ligase complexes at least composed of ASK1 or ASK2, CUL1, RBX1A or RBX1B and COI1. Interacts with ASK1 and ASK2, but separately, Binds also to ASK11 and ASK12. Interacts with RBCS-1B and HDA6. SCF complexes interact with the COP9 signalosome (CSN). Interacts with TIFY10A. Ref.10 Ref.11 Ref.13 Ref.15 Ref.17
Domain	The F-box domain is essential for the formation of SFC(COI1) complexes. Ref.12 The Leu-rich domain is involved in the interactions with RBCS-1B and RPD3B. Ref.12
Disruption phenotype	Mutants coi1-1 to coi1-14 are male sterile, insensitive to MeJA and coronatine, and exhibit enhanced resistance to Pseudomonas syringae atropurpurea (coronatine producing strain). Mutant coi1-16 has reduced sensitivity to jasmonate, but is male fertile when grown below 22 degrees Celsius and male sterile otherwise. Ref.1
Sequence similarities	Contains 1 F-box domain. Contains 18 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Jasmonic acid signaling pathway Plant defense Ubl conjugation pathway
Domain	Leucine-rich repeat Repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from mutant phenotype. Source: TAIR defense response to bacterium Inferred from mutant phenotype. Source: TAIR defense response to fungus Inferred from mutant phenotype. Source: TAIR jasmonic acid and ethylene-dependent systemic resistance Traceable author statement. Source: TAIR jasmonic acid mediated signaling pathway Inferred from genetic interaction. Source: TAIR negative regulation of defense response Inferred from mutant phenotype. Source: TAIR regulation of flower development Inferred from mutant phenotype. Source: TAIR response to far red light Inferred from mutant phenotype. Source: TAIR response to insect Inferred from mutant phenotype. Source: TAIR shade avoidance Inferred from mutant phenotype. Source: TAIR stomatal movement Inferred from mutant phenotype. Source: TAIR
Cellular component	SCF ubiquitin ligase complex Inferred from physical interaction. Source: TAIR
Molecular function	protein binding Inferred from physical interaction Ref.10Ref.11Ref.15. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CUL1	Q94AH6	3	EBI-401159,EBI-532411
HDA6	Q9FML2	3	EBI-401159,EBI-639608
SKP1A	Q39255	7	EBI-401159,EBI-532357
SKP1B	Q9FHW7	6	EBI-401159,EBI-604076
TIFY10A	Q9LMA8	5	EBI-401159,EBI-1388539
TIFY6B	Q9LVI4	6	EBI-401159,EBI-1792431
TIFY7	Q8W4J8	5	EBI-401159,EBI-1792583

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 592

592

Coronatine-insensitive protein 1

PRO_0000119960

Regions

Domain

16 – 57

42

F-box

Repeat

58 – 82

25

LRR 1

Repeat

83 – 102

20

LRR 2

Repeat

103 – 120

18

LRR 3

Repeat

121 – 154

34

LRR 4

Repeat

155 – 182

28

LRR 5

Repeat

183 – 210

28

LRR 6

Repeat

211 – 236

26

LRR 7

Repeat

237 – 264

28

LRR 8

Repeat

265 – 283

19

LRR 8

Repeat

284 – 308

25

LRR 10

Repeat

309 – 332

24

LRR 11

Repeat

333 – 368

36

LRR 12

Repeat

369 – 393

25

LRR 13

Repeat

394 – 426

33

LRR 14

Repeat

427 – 456

30

LRR 15

Repeat

457 – 478

22

LRR 16

Repeat

479 – 500

22

LRR 17

Repeat

501 – 524

24

LRR 18

Compositional bias

102 – 517

416

Leu-rich

Sites

Binding site

85

1

Jasmonate

Binding site

348

1

Jasmonate

Binding site

350

1

Jasmonate

Binding site

386

1

Jasmonate

Binding site

409

1

Jasmonate

Binding site

496

1

Jasmonate

Experimental info

Mutagenesis

11

1

L → A: No effects on interactions. Ref.11

Mutagenesis

22

1

E → A: Abrogates SFC(COI1) complexes formation, loss of response to jasmonate. Ref.10

Mutagenesis

44

1

W → A: Abrogates SFC(COI1) complexes formation and of interactions with RBCS-1B and RPD3B, loss of response to jasmonate. Ref.11

Mutagenesis

85

1

R → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

88

1

M → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

89

1

F → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

121

1

R → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

245

1

L → F in coi1-16; abrogates interactions with RBCS-1B and RPD3B (coi1-16). Ref.9 Ref.11

Mutagenesis

301

1

L → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

302

1

Y → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

326

1

R → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

348

1

R → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

351

1

R → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

386

1

Y → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

409

1

R → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

444

1

Y → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

469

1

L → A: Loss of interaction with TIFY10A. Ref.17

Mutagenesis

496

1

R → A: Loss of interaction with TIFY10A. Ref.17

Secondary structure

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592

Helix Strand Turn

Details...

Helix

17 – 19

3

Helix

21 – 25

5

Helix

31 – 37

7

Helix

42 – 51

10

Beta strand

54 – 58

5

Helix

60 – 62

3

Helix

65 – 71

7

Beta strand

76 – 80

5

Helix

85 – 89

5

Helix

101 – 110

10

Beta strand

116 – 121

6

Helix

126 – 136

11

Helix

137 – 139

3

Beta strand

142 – 147

6

Helix

157 – 162

6

Beta strand

167 – 170

4

Helix

183 – 190

8

Beta strand

196 – 198

3

Helix

209 – 218

10

Beta strand

224 – 226

3

Helix

232 – 235

4

Turn

239 – 241

3

Beta strand

247 – 249

3

Beta strand

262 – 264

3

Helix

285 – 294

10

Beta strand

297 – 300

4

Helix

307 – 314

8

Beta strand

322 – 326

5

Helix

335 – 340

6

Beta strand

346 – 350

5

Helix

371 – 374

4

Beta strand

381 – 388

8

Helix

392 – 397

6

Beta strand

407 – 412

6

Helix

429 – 432

4

Beta strand

440 – 444

5

Beta strand

467 – 470

4

Helix

481 – 484

4

Beta strand

492 – 495

4

Helix

502 – 511

10

Beta strand

517 – 520

4

Helix

532 – 535

4

Beta strand

540 – 545

6

Beta strand

567 – 572

6

Sequences

	Sequence		Length	Mass (Da)
	O04197 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 1DDCF04990144C06	FASTA	592	67,665
	10 20 30 40 50 60 MEDPDIKRCK LSCVATVDDV IEQVMTYITD PKDRDSASLV CRRWFKIDSE TREHVTMALC 70 80 90 100 110 120 YTATPDRLSR RFPNLRSLKL KGKPRAAMFN LIPENWGGYV TPWVTEISNN LRQLKSVHFR 130 140 150 160 170 180 RMIVSDLDLD RLAKARADDL ETLKLDKCSG FTTDGLLSIV THCRKIKTLL MEESSFSEKD 190 200 210 220 230 240 GKWLHELAQH NTSLEVLNFY MTEFAKISPK DLETIARNCR SLVSVKVGDF EILELVGFFK 250 260 270 280 290 300 AAANLEEFCG GSLNEDIGMP EKYMNLVFPR KLCRLGLSYM GPNEMPILFP FAAQIRKLDL 310 320 330 340 350 360 LYALLETEDH CTLIQKCPNL EVLETRNVIG DRGLEVLAQY CKQLKRLRIE RGADEQGMED 370 380 390 400 410 420 EEGLVSQRGL IALAQGCQEL EYMAVYVSDI TNESLESIGT YLKNLCDFRL VLLDREERIT 430 440 450 460 470 480 DLPLDNGVRS LLIGCKKLRR FAFYLRQGGL TDLGLSYIGQ YSPNVRWMLL GYVGESDEGL 490 500 510 520 530 540 MEFSRGCPNL QKLEMRGCCF SERAIAAAVT KLPSLRYLWV QGYRASMTGQ DLMQMARPYW 550 560 570 580 590 NIELIPSRRV PEVNQQGEIR EMEHPAHILA YYSLAGQRTD CPTTVRVLKE PI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility." Xie D., Feys B.F., James S., Nieto-Rostro M., Turner J.G. Science 280:1091-1094(1998) [PubMed: 9582125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE. Strain: cv. Columbia.
[2]	"Genes encoding defense signaling proteins in plants show weaker signatures of selection than those encoding recognition proteins." Caldwell K.S., Michelmore R.W. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Aa-0, cv. Ak-1, cv. Bay-0, cv. C24, cv. Columbia, cv. Cvi-0, cv. Di-0, cv. Ei-2, cv. Gu-0, cv. HOG, cv. Landsberg erecta, cv. Lz-0, cv. Nd-1, cv. Sha, cv. Sorbo, cv. Tsu-0, cv. Wassilewskija and cv. Wei-0.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen." Feys B.J.F., Benedetti C.S., Penfold C.N., Turner J.G. Plant Cell 6:751-759(1994) [PubMed: 12244256] [Abstract] Cited for: FUNCTION. Strain: cv. Columbia.
[7]	"Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis." Reymond P., Weber H., Damond M., Farmer E.E. Plant Cell 12:707-720(2000) [PubMed: 10810145] [Abstract] Cited for: FUNCTION.
[8]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[9]	"A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings." Ellis C., Turner J.G. Planta 215:549-556(2002) [PubMed: 12172836] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LEU-245.
[10]	"The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis." Xu L., Liu F., Lechner E., Genschik P., Crosby W.L., Ma H., Peng W., Huang D., Xie D. Plant Cell 14:1919-1935(2002) [PubMed: 12172031] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF GLU-22.
[11]	"COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis." Devoto A., Nieto-Rostro M., Xie D., Ellis C., Harmston R., Patrick E., Davis J., Sherratt L., Coleman M., Turner J.G. Plant J. 32:457-466(2002) [PubMed: 12445118] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF LEU-11; TRP-44 AND LEU-245.
[12]	"Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression." Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H. Plant Mol. Biol. 49:69-79(2002) [PubMed: 12008900] [Abstract] Cited for: DOMAIN LEUCINE-RICH REPEATS.
[13]	"The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses." Feng S., Ma L., Wang X., Xie D., Dinesh-Kumar S.P., Wei N., Deng X.W. Plant Cell 15:1083-1094(2003) [PubMed: 12724535] [Abstract] Cited for: FUNCTION, SUBUNIT.
[14]	"Characterization of the early response of Arabidopsis to Alternaria brassicicola infection using expression profiling." van Wees S.C.M., Chang H.-S., Zhu T., Glazebrook J. Plant Physiol. 132:606-617(2003) [PubMed: 12805591] [Abstract] Cited for: FUNCTION.
[15]	"Expression and interaction analysis of Arabidopsis Skp1-related genes." Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K., Shimada H., Matsui M. Plant Cell Physiol. 45:83-91(2004) [PubMed: 14749489] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK11 AND ASK12.
[16]	"Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine." He P., Chintamanani S., Chen Z., Zhu L., Kunkel B.N., Alfano J.R., Tang X., Zhou J.-M. Plant J. 37:589-602(2004) [PubMed: 14756769] [Abstract] Cited for: FUNCTION.
[17]	"Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor." Sheard L.B., Tan X., Mao H., Withers J., Ben-Nissan G., Hinds T.R., Kobayashi Y., Hsu F.F., Sharon M., Browse J., He S.Y., Rizo J., Howe G.A., Zheng N. Nature 468:400-405(2010) [PubMed: 20927106] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS)1-592 IN COMPLEX WITH JASMONOYL-ISOLEUCINE AND CORONATINE, INTERACTION WITH SKP1A AND TIFY10A, MUTAGENESIS OF ARG-85; MET-88; PHE-89; ARG-121; LEU-301; TYR-302; ARG-326; ARG-348; ARG-351; TYR-386; ARG-409; TYR-444; LEU-469 AND ARG-496.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF036340 mRNA. Translation: AAC17498.1.
EF470606 Genomic DNA. Translation: ABR45936.1.
EF470607 Genomic DNA. Translation: ABR45937.1.
EF470608 Genomic DNA. Translation: ABR45938.1.
EF470609 Genomic DNA. Translation: ABR45939.1.
EF470610 Genomic DNA. Translation: ABR45940.1.
EF470611 Genomic DNA. Translation: ABR45941.1.
EF470612 Genomic DNA. Translation: ABR45942.1.
EF470613 Genomic DNA. Translation: ABR45943.1.
EF470614 Genomic DNA. Translation: ABR45944.1.
EF470615 Genomic DNA. Translation: ABR45945.1.
EF470616 Genomic DNA. Translation: ABR45946.1.
EF470617 Genomic DNA. Translation: ABR45947.1.
EF470619 Genomic DNA. Translation: ABR45949.1.
EF470620 Genomic DNA. Translation: ABR45950.1.
EF470621 Genomic DNA. Translation: ABR45951.1.
EF470622 Genomic DNA. Translation: ABR45952.1.
EF470623 Genomic DNA. Translation: ABR45953.1.
EF470624 Genomic DNA. Translation: ABR45954.1.
AF002109 Genomic DNA. Translation: AAB95279.1.
CP002685 Genomic DNA. Translation: AEC09753.1.
AY045625 mRNA. Translation: AAK73983.1.
AY133556 mRNA. Translation: AAM91386.1.

IPI

IPI00516922.

PIR

T52139.

RefSeq

NP_565919.1. NM_129552.3.

UniGene

At.20831.
At.71018.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3OGK	X-ray	2.80	B/D/F/H/J/L/N/P	1-592	[»]
3OGL	X-ray	3.18	B/D/F/H/J/L/N/P	1-592	[»]
3OGM	X-ray	3.34	B/D/F/H/J/L/N/P	1-592	[»]

ProteinModelPortal

O04197.

SMR

O04197. Positions 12-592.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31324N.

IntAct

O04197. 13 interactions.

STRING

O04197.

Proteomic databases

PRIDE

O04197.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT2G39940.1; AT2G39940.1; AT2G39940.

GeneID

818581.

GenomeReviews

Gene locus AT2G39940 in contig CT485783_GR.

KEGG

ath:AT2G39940.

NMPDR

fig|3702.1.peg.11117.

Organism-specific databases

GeneFarm

4758.

TAIR

At2g39940.

Phylogenomic databases

GeneTree

EPGT00070000029218.

HOGENOM

HBG319412.

InParanoid

O04197.

OMA

ARNCRSL.

PhylomeDB

O04197.

ProtClustDB

CLSN2688817.

Gene expression databases

ArrayExpress

O04197.

Genevestigator

O04197.

GermOnline

AT2G39940. Arabidopsis thaliana.

Family and domain databases

KO

K13463.

PROSITE

PS50181. FBOX. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

COI1_ARATH

Accession

Primary (citable) accession number: O04197
Secondary accession number(s): B2BD84

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 1, 1997
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program