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Protein

Isoamylase 1, chloroplastic

Gene

ISA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the trimming of pre-amylopectin chains. Accelerates the crystallization of nascent amylopectin molecules during starch synthesis. ISA1 and ISA2 work exclusively together as a multimeric holoenzyme. ISA1-ISA2 removes preferentially branches that are very close to other branches.3 Publications

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei410 – 4101NucleophileBy similarity
Active sitei466 – 4661Proton donorBy similarity
Sitei538 – 5381Transition state stabilizerBy similarity

GO - Molecular functioni

  • cation binding Source: InterPro
  • isoamylase activity Source: TAIR

GO - Biological processi

  • amylopectin biosynthetic process Source: TAIR
  • glycogen metabolic process Source: GOC
  • starch biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Starch biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G39930-MONOMER.
BRENDAi3.2.1.68. 399.
UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoamylase 1, chloroplastic (EC:3.2.1.68)
Short name:
AtISA1
Gene namesi
Name:ISA1
Ordered Locus Names:At2g39930
ORF Names:T28M21.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G39930.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast isoamylase complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Strong reduction of the starch level in leaves, but 50-fold increase of water-soluble polysaccharides. No alteration of the amylase-to-amylopectin ratio.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343ChloroplastSequence analysisAdd
BLAST
Chaini44 – 783740Isoamylase 1, chloroplasticPRO_0000379527Add
BLAST

Proteomic databases

PaxDbiO04196.
PRIDEiO04196.

Expressioni

Gene expression databases

GenevisibleiO04196. AT.

Interactioni

Subunit structurei

Associates with ISA2 to form the heteromultimeric complex Iso1 required for amylopectin synthesis.

Protein-protein interaction databases

BioGridi3918. 2 interactions.
IntActiO04196. 2 interactions.
STRINGi3702.AT2G39930.1.

Structurei

3D structure databases

ProteinModelPortaliO04196.
SMRiO04196. Positions 72-783.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi549 – 57123Asn-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IN7N. Eukaryota.
COG1523. LUCA.
HOGENOMiHOG000239197.
InParanoidiO04196.
KOiK01214.
OMAiYAQDNPI.
PhylomeDBiO04196.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAIKCSSSF LHHTKLNTLF SNHTFPKISA PNFKPLFRPI SISAKDRRSN
60 70 80 90 100
EAENIAVVEK PLKSDRFFIS DGLPSPFGPT VRDDGVNFSV YSTNSVSATI
110 120 130 140 150
CLISLSDLRQ NKVTEEIQLD PSRNRTGHVW HVFLRGDFKD MLYGYRFDGK
160 170 180 190 200
FSPEEGHYYD SSNILLDPYA KAIISRDEFG VLGPDDNCWP QMACMVPTRE
210 220 230 240 250
EEFDWEGDMH LKLPQKDLVI YEMHVRGFTR HESSKIEFPG TYQGVAEKLD
260 270 280 290 300
HLKELGINCI ELMPCHEFNE LEYYSYNTIL GDHRVNFWGY STIGFFSPMI
310 320 330 340 350
RYASASSNNF AGRAINEFKI LVKEAHKRGI EVIMDVVLNH TAEGNEKGPI
360 370 380 390 400
FSFRGVDNSV YYMLAPKGEF YNYSGCGNTF NCNHPVVRQF ILDCLRYWVT
410 420 430 440 450
EMHVDGFRFD LGSIMSRSSS LWDAANVYGA DVEGDLLTTG TPISCPPVID
460 470 480 490 500
MISNDPILRG VKLIAEAWDA GGLYQVGMFP HWGIWSEWNG KFRDVVRQFI
510 520 530 540 550
KGTDGFSGAF AECLCGSPNL YQGGRKPWHS INFICAHDGF TLADLVTYNN
560 570 580 590 600
KNNLANGEEN NDGENHNYSW NCGEEGDFAS ISVKRLRKRQ MRNFFVSLMV
610 620 630 640 650
SQGVPMIYMG DEYGHTKGGN NNTYCHDNYM NYFRWDKKEE AHSDFFRFCR
660 670 680 690 700
ILIKFRDECE SLGLNDFPTA KRLQWHGLAP EIPNWSETSR FVAFSLVDSV
710 720 730 740 750
KKEIYVAFNT SHLATLVSLP NRPGYRWEPF VDTSKPSPYD CITPDLPERE
760 770 780
TAMKQYRHFL DANVYPMLSY SSIILLLSPI KDP
Length:783
Mass (Da):89,481
Last modified:July 1, 1997 - v1
Checksum:i638A6F9088CDB896
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002109 Genomic DNA. Translation: AAB95278.1.
CP002685 Genomic DNA. Translation: AEC09752.1.
BT000443 mRNA. Translation: AAN17420.1.
BT010348 mRNA. Translation: AAQ56791.1.
PIRiB84823.
RefSeqiNP_181522.1. NM_129551.3.
UniGeneiAt.20831.
At.71018.

Genome annotation databases

EnsemblPlantsiAT2G39930.1; AT2G39930.1; AT2G39930.
GeneIDi818580.
GrameneiAT2G39930.1; AT2G39930.1; AT2G39930.
KEGGiath:AT2G39930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002109 Genomic DNA. Translation: AAB95278.1.
CP002685 Genomic DNA. Translation: AEC09752.1.
BT000443 mRNA. Translation: AAN17420.1.
BT010348 mRNA. Translation: AAQ56791.1.
PIRiB84823.
RefSeqiNP_181522.1. NM_129551.3.
UniGeneiAt.20831.
At.71018.

3D structure databases

ProteinModelPortaliO04196.
SMRiO04196. Positions 72-783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3918. 2 interactions.
IntActiO04196. 2 interactions.
STRINGi3702.AT2G39930.1.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiO04196.
PRIDEiO04196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G39930.1; AT2G39930.1; AT2G39930.
GeneIDi818580.
GrameneiAT2G39930.1; AT2G39930.1; AT2G39930.
KEGGiath:AT2G39930.

Organism-specific databases

TAIRiAT2G39930.

Phylogenomic databases

eggNOGiENOG410IN7N. Eukaryota.
COG1523. LUCA.
HOGENOMiHOG000239197.
InParanoidiO04196.
KOiK01214.
OMAiYAQDNPI.
PhylomeDBiO04196.

Enzyme and pathway databases

UniPathwayiUPA00152.
BioCyciARA:AT2G39930-MONOMER.
BRENDAi3.2.1.68. 399.

Miscellaneous databases

PROiO04196.

Gene expression databases

GenevisibleiO04196. AT.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis mutants Atisa1 and Atisa2 have identical phenotypes and lack the same multimeric isoamylase, which influences the branch point distribution of amylopectin during starch synthesis."
    Delatte T., Trevisan M., Parker M.L., Zeeman S.C.
    Plant J. 41:815-830(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ISA2, DISRUPTION PHENOTYPE.
  5. "Mutants of Arabidopsis lacking a chloroplastic isoamylase accumulate phytoglycogen and an abnormal form of amylopectin."
    Wattebled F., Dong Y., Dumez S., Delvalle D., Planchot V., Berbezy P., Vyas D., Colonna P., Chatterjee M., Ball S., D'Hulst C.
    Plant Physiol. 138:184-195(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ISA2, DISRUPTION PHENOTYPE.
  6. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase."
    Streb S., Delatte T., Umhang M., Eicke S., Schorderet M., Reinhardt D., Zeeman S.C.
    Plant Cell 20:3448-3466(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Further evidence for the mandatory nature of polysaccharide debranching for the aggregation of semicrystalline starch and for overlapping functions of debranching enzymes in Arabidopsis leaves."
    Wattebled F., Planchot V., Dong Y., Szydlowski N., Pontoire B., Devin A., Ball S., D'Hulst C.
    Plant Physiol. 148:1309-1323(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiISOA1_ARATH
AccessioniPrimary (citable) accession number: O04196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ISA2, ISA1 may be unstable.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.