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Protein

Isoamylase 1, chloroplastic

Gene

ISA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the trimming of pre-amylopectin chains. Accelerates the crystallization of nascent amylopectin molecules during starch synthesis. ISA1 and ISA2 work exclusively together as a multimeric holoenzyme. ISA1-ISA2 removes preferentially branches that are very close to other branches.3 Publications

Miscellaneous

In the absence of ISA2, ISA1 may be unstable.

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei410NucleophileBy similarity1
Active sitei466Proton donorBy similarity1
Sitei538Transition state stabilizerBy similarity1

GO - Molecular functioni

  • isoamylase activity Source: TAIR

GO - Biological processi

  • amylopectin biosynthetic process Source: TAIR
  • starch biosynthetic process Source: UniProtKB-UniPathway
  • starch catabolic process Source: EnsemblPlants

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Starch biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G39930-MONOMER
BRENDAi3.2.1.68 399
UniPathwayiUPA00152

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Isoamylase 1, chloroplastic (EC:3.2.1.68)
Short name:
AtISA1
Gene namesi
Name:ISA1
Ordered Locus Names:At2g39930
ORF Names:T28M21.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G39930
TAIRilocus:2061216 AT2G39930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Strong reduction of the starch level in leaves, but 50-fold increase of water-soluble polysaccharides. No alteration of the amylase-to-amylopectin ratio.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43ChloroplastSequence analysisAdd BLAST43
ChainiPRO_000037952744 – 783Isoamylase 1, chloroplasticAdd BLAST740

Proteomic databases

PaxDbiO04196

Expressioni

Gene expression databases

ExpressionAtlasiO04196 baseline and differential
GenevisibleiO04196 AT

Interactioni

Subunit structurei

Associates with ISA2 to form the heteromultimeric complex Iso1 required for amylopectin synthesis.

Protein-protein interaction databases

BioGridi3918, 2 interactors
IntActiO04196, 2 interactors
STRINGi3702.AT2G39930.1

Structurei

3D structure databases

ProteinModelPortaliO04196
SMRiO04196
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi549 – 571Asn-richAdd BLAST23

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IN7N Eukaryota
COG1523 LUCA
HOGENOMiHOG000239197
InParanoidiO04196
KOiK01214
OMAiHAFVNDQ
OrthoDBiEOG093603M5
PhylomeDBiO04196

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02922 CBM_48, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 3 hits
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAIKCSSSF LHHTKLNTLF SNHTFPKISA PNFKPLFRPI SISAKDRRSN
60 70 80 90 100
EAENIAVVEK PLKSDRFFIS DGLPSPFGPT VRDDGVNFSV YSTNSVSATI
110 120 130 140 150
CLISLSDLRQ NKVTEEIQLD PSRNRTGHVW HVFLRGDFKD MLYGYRFDGK
160 170 180 190 200
FSPEEGHYYD SSNILLDPYA KAIISRDEFG VLGPDDNCWP QMACMVPTRE
210 220 230 240 250
EEFDWEGDMH LKLPQKDLVI YEMHVRGFTR HESSKIEFPG TYQGVAEKLD
260 270 280 290 300
HLKELGINCI ELMPCHEFNE LEYYSYNTIL GDHRVNFWGY STIGFFSPMI
310 320 330 340 350
RYASASSNNF AGRAINEFKI LVKEAHKRGI EVIMDVVLNH TAEGNEKGPI
360 370 380 390 400
FSFRGVDNSV YYMLAPKGEF YNYSGCGNTF NCNHPVVRQF ILDCLRYWVT
410 420 430 440 450
EMHVDGFRFD LGSIMSRSSS LWDAANVYGA DVEGDLLTTG TPISCPPVID
460 470 480 490 500
MISNDPILRG VKLIAEAWDA GGLYQVGMFP HWGIWSEWNG KFRDVVRQFI
510 520 530 540 550
KGTDGFSGAF AECLCGSPNL YQGGRKPWHS INFICAHDGF TLADLVTYNN
560 570 580 590 600
KNNLANGEEN NDGENHNYSW NCGEEGDFAS ISVKRLRKRQ MRNFFVSLMV
610 620 630 640 650
SQGVPMIYMG DEYGHTKGGN NNTYCHDNYM NYFRWDKKEE AHSDFFRFCR
660 670 680 690 700
ILIKFRDECE SLGLNDFPTA KRLQWHGLAP EIPNWSETSR FVAFSLVDSV
710 720 730 740 750
KKEIYVAFNT SHLATLVSLP NRPGYRWEPF VDTSKPSPYD CITPDLPERE
760 770 780
TAMKQYRHFL DANVYPMLSY SSIILLLSPI KDP
Length:783
Mass (Da):89,481
Last modified:July 1, 1997 - v1
Checksum:i638A6F9088CDB896
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002109 Genomic DNA Translation: AAB95278.1
CP002685 Genomic DNA Translation: AEC09752.1
BT000443 mRNA Translation: AAN17420.1
BT010348 mRNA Translation: AAQ56791.1
PIRiB84823
RefSeqiNP_181522.1, NM_129551.4
UniGeneiAt.20831
At.71018

Genome annotation databases

EnsemblPlantsiAT2G39930.1; AT2G39930.1; AT2G39930
GeneIDi818580
GrameneiAT2G39930.1; AT2G39930.1; AT2G39930
KEGGiath:AT2G39930

Similar proteinsi

Entry informationi

Entry nameiISOA1_ARATH
AccessioniPrimary (citable) accession number: O04196
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: July 1, 1997
Last modified: April 25, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health