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Protein

Ras-related protein RABG3b

Gene

RABG3B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Intracellular vesicle trafficking and protein transport. Functions in autophagy. Involved in xylem and tracheary element differentiation.1 Publication

Miscellaneous

Plants silencing RABG3B display a significant decrease in the number of both protoxylem and metaxylem cells in the basal region of inflorescence stems.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi125 – 128GTPBy similarity4
Nucleotide bindingi158 – 159GTPBy similarity2

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: TAIR

GO - Biological processi

  • positive regulation of autophagy Source: UniProtKB
  • programmed cell death involved in cell development Source: TAIR
  • protein transport Source: UniProtKB-KW
  • tracheary element differentiation Source: TAIR
  • xylem development Source: UniProtKB

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-8854214. TBC/RABGAPs.
R-ATH-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein RABG3b
Short name:
AtRABG3b
Alternative name(s):
Ras-related protein Rab75
Short name:
AtRab75
Gene namesi
Name:RABG3B
Synonyms:RAB7, RAB75
Ordered Locus Names:At1g22740
ORF Names:T22J18.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G22740.
TAIRilocus:2199716. AT1G22740.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: UniProtKB-SubCell
  • vacuole Source: TAIR

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi22T → N: Dominant negative (GDP-bound form); inhibits autophagy and tracheary element formation. 1 Publication1
Mutagenesisi67Q → L: Constitutively active (GTP bound form); stimulates autophagy and tracheary element formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001212802 – 203Ras-related protein RABG3bAdd BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi201S-geranylgeranyl cysteineBy similarity1
Modified residuei203Cysteine methyl esterBy similarity1
Lipidationi203S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiO04157.
PRIDEiO04157.

Expressioni

Tissue specificityi

Expressed in xylem cells of inflorescence stems.1 Publication

Gene expression databases

ExpressionAtlasiO04157. baseline and differential.
GenevisibleiO04157. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G22740.1.

Structurei

3D structure databases

ProteinModelPortaliO04157.
SMRiO04157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
HOGENOMiHOG000233968.
InParanoidiO04157.
KOiK07897.
OMAiPPDPKTF.
OrthoDBiEOG09360LTD.
PhylomeDBiO04157.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRRRTLLK VIILGDSGVG KTSLMNQYVN NKFSQQYKAT IGADFVTKEL
60 70 80 90 100
QIDDRLVTLQ IWDTAGQERF QSLGVAFYRG ADCCVLVYDV NHLKSFESLD
110 120 130 140 150
NWHNEFLTRA SPRDPMAFPF ILLGNKVDID GGNSRVVSEK KAREWCAEKG
160 170 180 190 200
NIVYFETSAK EDYNVDDSFL CITKLALANE RDQDIYFQPD TGSVPEQRGG

CAC
Length:203
Mass (Da):22,945
Last modified:July 1, 1997 - v1
Checksum:i18FAA93B2709C14A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071850 mRNA. Translation: BAB68375.1.
Y09821 mRNA. Translation: CAA70951.1.
Y12227 Genomic DNA. Translation: CAA72904.1.
AC003979 Genomic DNA. Translation: AAC25512.1.
CP002684 Genomic DNA. Translation: AEE30279.1.
BT024589 mRNA. Translation: ABD42987.1.
PIRiT00770.
RefSeqiNP_173688.1. NM_102121.3.
UniGeneiAt.20067.

Genome annotation databases

EnsemblPlantsiAT1G22740.1; AT1G22740.1; AT1G22740.
GeneIDi838880.
GrameneiAT1G22740.1; AT1G22740.1; AT1G22740.
KEGGiath:AT1G22740.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRAG3B_ARATH
AccessioniPrimary (citable) accession number: O04157
Secondary accession number(s): Q2HIJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: May 10, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families