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Protein

Calreticulin-3

Gene

CRT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Required for elongation factor Tu receptor (EFR) accumulation and for EFR, but not flagellin-sensing 2 (FLS2) signaling.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118CarbohydrateBy similarity1
Binding sitei120CarbohydrateBy similarity1
Binding sitei137CarbohydrateBy similarity1
Binding sitei144CarbohydrateBy similarity1
Binding sitei327CarbohydrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • anthocyanin-containing compound metabolic process Source: TAIR
  • defense response signaling pathway, resistance gene-independent Source: TAIR
  • defense response to bacterium Source: TAIR
  • plant-type hypersensitive response Source: TAIR
  • protein folding Source: TAIR

Keywordsi

Molecular functionChaperone
LigandCalcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-901042 Calnexin/calreticulin cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin-3
Alternative name(s):
Protein PRIORITY IN SWEET LIFE 1
Gene namesi
Name:CRT3
Ordered Locus Names:At1g08450
ORF Names:T27G7.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G08450
TAIRilocus:2201816 AT1G08450

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Loss of seedling growth inhibition in response to the pathogen-associated molecular pattern (PAMP) elf18 and increased susceptibility to phytopathogenic bacteria.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67P → L in psl1-1; no effect on EFR accumulation, but decreased response to the PAMP elf18. 1 Publication1
Mutagenesisi115G → D in crt3-3; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication1
Mutagenesisi142G → E in crt3-4; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication1
Mutagenesisi147G → E in crt3-5; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication1
Mutagenesisi179H → Y in crt3-6; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication1
Mutagenesisi203G → D in crt3-7; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication1
Mutagenesisi244G → S in psl1-3; no effect on EFR accumulation, but decreased response to the PAMP elf18. 1 Publication1
Mutagenesisi293P → S in crt3-10; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication1
Mutagenesisi335S → L in psl1-4; loss of EFR accumulation and loss of response to the PAMP elf18. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000000418629 – 424Calreticulin-3Add BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi114 ↔ 146By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO04153
PRIDEiO04153

Expressioni

Gene expression databases

ExpressionAtlasiO04153 baseline and differential
GenevisibleiO04153 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi22606, 1 interactor
DIPiDIP-48930N
IntActiO04153, 1 interactor
STRINGi3702.AT1G08450.1

Structurei

3D structure databases

ProteinModelPortaliO04153
SMRiO04153
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati200 – 2111-1Add BLAST12
Repeati219 – 2301-2Add BLAST12
Repeati236 – 2471-3Add BLAST12
Repeati254 – 2651-4Add BLAST12
Repeati269 – 2792-1Add BLAST11
Repeati283 – 2932-2Add BLAST11
Repeati297 – 3072-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni200 – 2654 X approximate repeatsAdd BLAST66
Regioni269 – 3073 X approximate repeatsAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi421 – 424Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi361 – 414Arg/Glu/Lys-richAdd BLAST54

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674 Eukaryota
ENOG410XRR7 LUCA
HOGENOMiHOG000192435
InParanoidiO04153
KOiK08057
OMAiQEDMHSE
OrthoDBiEOG09360DY8
PhylomeDBiO04153

Family and domain databases

Gene3Di2.10.250.10, 1 hit
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009169 Calreticulin
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 2 hits
PIRSFiPIRSF002356 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 1 hit
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit
PS00805 CALRETICULIN_REPEAT, 1 hit
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O04153-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLPQNKLSF FCFFFLVSVL TLAPLAFSEI FLEEHFEGGW KSRWVLSDWK
60 70 80 90 100
RNEGKAGTFK HTAGKWPGDP DNKGIQTYND AKHYAISAKI PEFSNKNRTL
110 120 130 140 150
VVQYSVKIEQ DIECGGAYIK LLSGYVNQKQ FGGDTPYSLM FGPDICGTQT
160 170 180 190 200
KKLHVIVSYQ GQNYPIKKDL QCETDKLNHF YTFILRPDAS YSVLVDNKER
210 220 230 240 250
EFGSMYTDWD ILPPRKIKVK NAKKPEDWDD REYIDDPNDV KPEGFDSIPR
260 270 280 290 300
EIPDRKAKEP EDWDEEENGL WEPPKIPNSA YKGPWKAKRI KNPNYKGKWK
310 320 330 340 350
NPWIDNPEFE DDPDLYVLKS IKYAGIEVWQ VKAGSIFDNI LICDDPAYAR
360 370 380 390 400
SIVDDYFAQH RESEKELFAE AEKERKARED EEARIAREEG ERRRKERDHR
410 420
YGDRRRRYKR PNPRDYMDDY HDEL
Length:424
Mass (Da):49,844
Last modified:February 5, 2008 - v2
Checksum:i64445A06359F0B8C
GO

Sequence cautioni

The sequence AAF22902 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279S → F in AAC49697 (PubMed:9159940).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66345 mRNA Translation: AAC49697.1
AC006932 Genomic DNA Translation: AAF22902.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE28291.1
AY056320 mRNA Translation: AAL07169.1
RefSeqiNP_563816.1, NM_100718.5 [O04153-1]
UniGeneiAt.24804

Genome annotation databases

EnsemblPlantsiAT1G08450.1; AT1G08450.1; AT1G08450 [O04153-1]
GeneIDi837365
GrameneiAT1G08450.1; AT1G08450.1; AT1G08450 [O04153-1]
KEGGiath:AT1G08450

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCALR3_ARATH
AccessioniPrimary (citable) accession number: O04153
Secondary accession number(s): Q93ZR4, Q9SJE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 5, 2008
Last modified: April 25, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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