Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O04153

- CALR3_ARATH

UniProt

O04153 - CALR3_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Calreticulin-3

Gene

CRT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Required for elongation factor Tu receptor (EFR) accumulation and for EFR, but not flagellin-sensing 2 (FLS2) signaling.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181CarbohydrateBy similarity
Binding sitei120 – 1201CarbohydrateBy similarity
Binding sitei137 – 1371CarbohydrateBy similarity
Binding sitei144 – 1441CarbohydrateBy similarity
Binding sitei327 – 3271CarbohydrateBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  1. anthocyanin-containing compound metabolic process Source: TAIR
  2. calcium ion homeostasis Source: TAIR
  3. defense response signaling pathway, resistance gene-independent Source: TAIR
  4. defense response to bacterium Source: TAIR
  5. plant-type hypersensitive response Source: TAIR
  6. protein folding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.
REACT_190967. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin-3
Alternative name(s):
Protein PRIORITY IN SWEET LIFE 1
Gene namesi
Name:CRT3
Ordered Locus Names:At1g08450
ORF Names:T27G7.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G08450.

Subcellular locationi

Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. endoplasmic reticulum membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Loss of seedling growth inhibition in response to the pathogen-associated molecular pattern (PAMP) elf18 and increased susceptibility to phytopathogenic bacteria.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi67 – 671P → L in psl1-1; no effect on EFR accumulation, but decreased response to the PAMP elf18. 1 Publication
Mutagenesisi115 – 1151G → D in crt3-3; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication
Mutagenesisi142 – 1421G → E in crt3-4; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication
Mutagenesisi147 – 1471G → E in crt3-5; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication
Mutagenesisi179 – 1791H → Y in crt3-6; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication
Mutagenesisi203 – 2031G → D in crt3-7; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication
Mutagenesisi244 – 2441G → S in psl1-3; no effect on EFR accumulation, but decreased response to the PAMP elf18. 1 Publication
Mutagenesisi293 – 2931P → S in crt3-10; loss of response to the PAMP elf18, but no effect on the response to PAMP flg22. 1 Publication
Mutagenesisi335 – 3351S → L in psl1-4; loss of EFR accumulation and loss of response to the PAMP elf18. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 424396Calreticulin-3PRO_0000004186Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi114 ↔ 146By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO04153.
PRIDEiO04153.

Expressioni

Gene expression databases

ExpressionAtlasiO04153. baseline and differential.
GenevestigatoriO04153.

Interactioni

Protein-protein interaction databases

DIPiDIP-48930N.

Structurei

3D structure databases

ProteinModelPortaliO04153.
SMRiO04153. Positions 30-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati200 – 211121-1Add
BLAST
Repeati219 – 230121-2Add
BLAST
Repeati236 – 247121-3Add
BLAST
Repeati254 – 265121-4Add
BLAST
Repeati269 – 279112-1Add
BLAST
Repeati283 – 293112-2Add
BLAST
Repeati297 – 307112-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni200 – 265664 X approximate repeatsAdd
BLAST
Regioni269 – 307393 X approximate repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi421 – 4244Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi361 – 41454Arg/Glu/Lys-richAdd
BLAST

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG295283.
HOGENOMiHOG000192435.
InParanoidiO04153.
KOiK08057.
OMAiFGSMYTD.
PhylomeDBiO04153.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O04153-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLPQNKLSF FCFFFLVSVL TLAPLAFSEI FLEEHFEGGW KSRWVLSDWK
60 70 80 90 100
RNEGKAGTFK HTAGKWPGDP DNKGIQTYND AKHYAISAKI PEFSNKNRTL
110 120 130 140 150
VVQYSVKIEQ DIECGGAYIK LLSGYVNQKQ FGGDTPYSLM FGPDICGTQT
160 170 180 190 200
KKLHVIVSYQ GQNYPIKKDL QCETDKLNHF YTFILRPDAS YSVLVDNKER
210 220 230 240 250
EFGSMYTDWD ILPPRKIKVK NAKKPEDWDD REYIDDPNDV KPEGFDSIPR
260 270 280 290 300
EIPDRKAKEP EDWDEEENGL WEPPKIPNSA YKGPWKAKRI KNPNYKGKWK
310 320 330 340 350
NPWIDNPEFE DDPDLYVLKS IKYAGIEVWQ VKAGSIFDNI LICDDPAYAR
360 370 380 390 400
SIVDDYFAQH RESEKELFAE AEKERKARED EEARIAREEG ERRRKERDHR
410 420
YGDRRRRYKR PNPRDYMDDY HDEL
Length:424
Mass (Da):49,844
Last modified:February 5, 2008 - v2
Checksum:i64445A06359F0B8C
GO

Sequence cautioni

The sequence AAF22902.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti279 – 2791S → F in AAC49697. (PubMed:9159940)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66345 mRNA. Translation: AAC49697.1.
AC006932 Genomic DNA. Translation: AAF22902.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28291.1.
AY056320 mRNA. Translation: AAL07169.1.
RefSeqiNP_563816.1. NM_100718.4. [O04153-1]
UniGeneiAt.24804.

Genome annotation databases

EnsemblPlantsiAT1G08450.1; AT1G08450.1; AT1G08450. [O04153-1]
GeneIDi837365.
KEGGiath:AT1G08450.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66345 mRNA. Translation: AAC49697.1 .
AC006932 Genomic DNA. Translation: AAF22902.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE28291.1 .
AY056320 mRNA. Translation: AAL07169.1 .
RefSeqi NP_563816.1. NM_100718.4. [O04153-1 ]
UniGenei At.24804.

3D structure databases

ProteinModelPortali O04153.
SMRi O04153. Positions 30-385.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48930N.

Proteomic databases

PaxDbi O04153.
PRIDEi O04153.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G08450.1 ; AT1G08450.1 ; AT1G08450 . [O04153-1 ]
GeneIDi 837365.
KEGGi ath:AT1G08450.

Organism-specific databases

TAIRi AT1G08450.

Phylogenomic databases

eggNOGi NOG295283.
HOGENOMi HOG000192435.
InParanoidi O04153.
KOi K08057.
OMAi FGSMYTD.
PhylomeDBi O04153.

Enzyme and pathway databases

Reactomei REACT_108190. ATF6-alpha activates chaperone genes.
REACT_190967. Calnexin/calreticulin cycle.

Gene expression databases

ExpressionAtlasi O04153. baseline and differential.
Genevestigatori O04153.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view ]
PANTHERi PTHR11073. PTHR11073. 1 hit.
Pfami PF00262. Calreticulin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002356. Calreticulin. 1 hit.
PRINTSi PR00626. CALRETICULIN.
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEi PS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Abundant accumulation of the calcium-binding molecular chaperone calreticulin in specific floral tissues of Arabidopsis thaliana."
    Nelson D.E., Glaunsinger B., Bohnert H.J.
    Plant Physiol. 114:29-37(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Receptor quality control in the endoplasmic reticulum for plant innate immunity."
    Saijo Y., Tintor N., Lu X., Rauf P., Pajerowska-Mukhtar K., Haeweker H., Dong X., Robatzek S., Schulze-Lefert P.
    EMBO J. 28:3439-3449(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF PRO-67; GLY-244 AND SER-335.
  6. "Specific ER quality control components required for biogenesis of the plant innate immune receptor EFR."
    Li J., Zhao-Hui C., Batoux M., Nekrasov V., Roux M., Chinchilla D., Zipfel C., Jones J.D.
    Proc. Natl. Acad. Sci. U.S.A. 106:15973-15978(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-115; GLY-142; GLY-147; HIS-179; GLY-203 AND PRO-293, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCALR3_ARATH
AccessioniPrimary (citable) accession number: O04153
Secondary accession number(s): Q93ZR4, Q9SJE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 5, 2008
Last modified: November 26, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3