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Protein

Calreticulin-1

Gene

CRT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112CarbohydrateBy similarity1
Binding sitei114CarbohydrateBy similarity1
Binding sitei131CarbohydrateBy similarity1
Binding sitei138CarbohydrateBy similarity1
Binding sitei321CarbohydrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • protein folding Source: InterPro
  • response to cadmium ion Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-901042. Calnexin/calreticulin cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin-1
Gene namesi
Name:CRT1
Ordered Locus Names:At1g56340
ORF Names:F13N6.20, F14G9.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G56340.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000000418423 – 425Calreticulin-1Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi108 ↔ 140By similarity
Glycosylationi154N-linked (GlcNAc...)Sequence analysis1
Modified residuei381PhosphoserineCombined sources1
Modified residuei397PhosphoserineBy similarity1
Glycosylationi399N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO04151.
PRIDEiO04151.

2D gel databases

SWISS-2DPAGEO04151.

PTM databases

iPTMnetiO04151.

Expressioni

Gene expression databases

ExpressionAtlasiO04151. baseline and differential.
GenevisibleiO04151. AT.

Interactioni

Protein-protein interaction databases

BioGridi27312. 4 interactors.
STRINGi3702.AT1G56340.1.

Structurei

3D structure databases

ProteinModelPortaliO04151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati194 – 2051-1Add BLAST12
Repeati213 – 2241-2Add BLAST12
Repeati230 – 2411-3Add BLAST12
Repeati248 – 2591-4Add BLAST12
Repeati263 – 2732-1Add BLAST11
Repeati277 – 2872-2Add BLAST11
Repeati291 – 3012-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 2594 X approximate repeatsAdd BLAST66
Regioni263 – 3013 X approximate repeatsAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi422 – 425Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi355 – 419Asp/Glu/Lys-richAdd BLAST65

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
HOGENOMiHOG000192435.
InParanoidiO04151.
KOiK08057.
OMAiDLDQADM.
OrthoDBiEOG09360F0J.
PhylomeDBiO04151.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O04151-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKLNPKFIS LILFALVVIV SAEVIFEEKF EDGWEKRWVK SDWKKDDNTA
60 70 80 90 100
GEWKHTAGNW SGDANDKGIQ TSEDYRFYAI SAEFPEFSNK DKTLVFQFSV
110 120 130 140 150
KHEQKLDCGG GYMKLLSDDV DQTKFGGDTP YSIMFGPDIC GYSTKKVHAI
160 170 180 190 200
LTYNGTNHLI KKEVPCETDQ LTHVYTFVLR PDATYSILID NVEKQTGSLY
210 220 230 240 250
SDWDLLPAKK IKDPSAKKPE DWDDKEYIPD PEDTKPAGYD DIPKEIPDTD
260 270 280 290 300
AKKPEDWDDE EDGEWTAPTI PNPEYNGEWK PKKIKNPAYK GKWKAPMIDN
310 320 330 340 350
PEFKDDPELY VFPKLKYVGV ELWQVKSGSL FDNVLVSDDP EYAKKLAEET
360 370 380 390 400
WGKHKDAEKA AFDEAEKKRE EEESKDAPAE SDAEEEAEDD DNEGDDSDNE
410 420
SKSEETKEAE ETKEAEETDA AHDEL
Length:425
Mass (Da):48,527
Last modified:July 1, 1997 - v1
Checksum:iBCEC08E2F342642E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66343 mRNA. Translation: AAC49695.1.
AC058785 Genomic DNA. Translation: AAG51504.1.
AC069159 Genomic DNA. Translation: AAG50908.1.
CP002684 Genomic DNA. Translation: AEE33379.1.
AY062628 mRNA. Translation: AAL32706.1.
BT008511 mRNA. Translation: AAP37870.1.
PIRiC96605.
RefSeqiNP_176030.1. NM_104513.5. [O04151-1]
UniGeneiAt.10808.

Genome annotation databases

EnsemblPlantsiAT1G56340.1; AT1G56340.1; AT1G56340. [O04151-1]
GeneIDi842087.
GrameneiAT1G56340.1; AT1G56340.1; AT1G56340.
KEGGiath:AT1G56340.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66343 mRNA. Translation: AAC49695.1.
AC058785 Genomic DNA. Translation: AAG51504.1.
AC069159 Genomic DNA. Translation: AAG50908.1.
CP002684 Genomic DNA. Translation: AEE33379.1.
AY062628 mRNA. Translation: AAL32706.1.
BT008511 mRNA. Translation: AAP37870.1.
PIRiC96605.
RefSeqiNP_176030.1. NM_104513.5. [O04151-1]
UniGeneiAt.10808.

3D structure databases

ProteinModelPortaliO04151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27312. 4 interactors.
STRINGi3702.AT1G56340.1.

PTM databases

iPTMnetiO04151.

2D gel databases

SWISS-2DPAGEO04151.

Proteomic databases

PaxDbiO04151.
PRIDEiO04151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G56340.1; AT1G56340.1; AT1G56340. [O04151-1]
GeneIDi842087.
GrameneiAT1G56340.1; AT1G56340.1; AT1G56340.
KEGGiath:AT1G56340.

Organism-specific databases

TAIRiAT1G56340.

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
HOGENOMiHOG000192435.
InParanoidiO04151.
KOiK08057.
OMAiDLDQADM.
OrthoDBiEOG09360F0J.
PhylomeDBiO04151.

Enzyme and pathway databases

ReactomeiR-ATH-901042. Calnexin/calreticulin cycle.

Miscellaneous databases

PROiO04151.

Gene expression databases

ExpressionAtlasiO04151. baseline and differential.
GenevisibleiO04151. AT.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 2 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALR1_ARATH
AccessioniPrimary (citable) accession number: O04151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.