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Protein

Chitinase 4

Gene

Cht4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes chitin and may function in reproductive organs during embryogenesis and seed maturation.1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  • chitinase activity Source: UniProtKB
  • chitin binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 4 (EC:3.2.1.14)
Alternative name(s):
OsChia2b
Pathogenesis related (PR)-3 chitinase 4
Gene namesi
Name:Cht4
Ordered Locus Names:Os04g0493400, LOC_Os04g41620
ORF Names:OsJ_15306, OSJNBb0091E11.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000038346328 – 285Chitinase 4Add BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 38PROSITE-ProRule annotation
Disulfide bondi32 ↔ 44PROSITE-ProRule annotation
Disulfide bondi37 ↔ 51PROSITE-ProRule annotation
Disulfide bondi55 ↔ 60PROSITE-ProRule annotation
Disulfide bondi104 ↔ 153PROSITE-ProRule annotation
Disulfide bondi166 ↔ 175PROSITE-ProRule annotation
Disulfide bondi253 ↔ 285PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO04138.
PRIDEiO04138.

Expressioni

Tissue specificityi

Expressed at low levels in leaves, sheaths and meristems.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os04g41620.1.

Structurei

3D structure databases

ProteinModelPortaliO04138.
SMRiO04138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 62Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiO04138.
OMAiDYCNGNT.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
Gene3Di3.30.60.10. 1 hit.
InterProiView protein in InterPro
IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR036861. Endochitinase-like_sf.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
PfamiView protein in Pfam
PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 2 hits.
PIRSFiPIRSF001060. Endochitinase. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609. Chitin_bd_1. 1 hit.
SMARTiView protein in SMART
SM00270. ChtBD1. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiView protein in PROSITE
PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O04138-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAKMATMVA LVFGLALLLS AAAPAAAQNC GCQDGYCCSQ WGYCGTTEAY
60 70 80 90 100
CGQGCQSGPC WGSGGEAAAG MAGRKAGAGA GVSVESVVTE AFFNGIKNQA
110 120 130 140 150
PNGCAGKSFY TRQSFLNAAR SYSGFANDRT NDDSKREIAA FFAHVTHETG
160 170 180 190 200
HMCYINEING ANMDYCDKSN KQWPCQPGKK YYGRGPLQIS WNFNYGPAGK
210 220 230 240 250
NIGFDGLRDP DKVAQDPTIS FKTALWFWMN NVHQVMSQGF GATIRAINGA
260 270 280
LECNGKNPGA VNARVNYYKD YCRQFGVSPG GNLYC
Note: Constructed according to the conserved gene model. No experimental confirmation available.
Length:285
Mass (Da):30,451
Last modified:September 22, 2009 - v2
Checksum:i289A6A44CB5EBFCE
GO
Isoform 2 (identifier: O04138-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     57-80: SGPCWGSGGEAAAGMAGRKAGAGA → MANSPTLTMLVFLAIGLSLVLSAA

Show »
Length:229
Mass (Da):25,151
Checksum:i90F7945DECDC7433
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0379981 – 56Missing in isoform 2. 2 PublicationsAdd BLAST56
Alternative sequenceiVSP_03799957 – 80SGPCW…AGAGA → MANSPTLTMLVFLAIGLSLV LSAA in isoform 2. 2 PublicationsAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054687 Genomic DNA. Translation: BAB21374.1.
AB003194 mRNA. Translation: BAA19793.1.
AL606629 Genomic DNA. Translation: CAD41540.2.
AP008210 Genomic DNA. Translation: BAF15098.1.
AP014960 Genomic DNA. Translation: BAS89866.1.
CM000141 Genomic DNA. Translation: EAZ31206.1.
AK060363 mRNA. Translation: BAG87422.1.
AK099973 mRNA. Translation: BAG94383.1.
PIRiT03405.
UniGeneiOs.28044.
Os.95634.

Genome annotation databases

EnsemblPlantsiOS04T0493400-01; OS04T0493400-01; OS04G0493400. [O04138-2]
GrameneiOS04T0493400-01; OS04T0493400-01; OS04G0493400.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCHI4_ORYSJ
AccessioniPrimary (citable) accession number: O04138
Secondary accession number(s): Q7XU64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: October 25, 2017
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families