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O04138

- CHI4_ORYSJ

UniProt

O04138 - CHI4_ORYSJ

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Protein
Chitinase 4
Gene
Cht4, Os04g0493400, LOC_Os04g41620, OsJ_15306, OSJNBb0091E11.8
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolyzes chitin and may function in reproductive organs during embryogenesis and seed maturation.1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
  2. chitinase activity Source: UniProtKB

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
  4. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 4 (EC:3.2.1.14)
Alternative name(s):
OsChia2b
Pathogenesis related (PR)-3 chitinase 4
Gene namesi
Name:Cht4
Ordered Locus Names:Os04g0493400, LOC_Os04g41620
ORF Names:OsJ_15306, OSJNBb0091E11.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 4

Organism-specific databases

GrameneiO04138.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Chaini28 – 285258Chitinase 4
PRO_0000383463Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 38 By similarity
Disulfide bondi32 ↔ 44 By similarity
Disulfide bondi37 ↔ 51 By similarity
Disulfide bondi55 ↔ 60 By similarity
Disulfide bondi104 ↔ 153 By similarity
Disulfide bondi166 ↔ 175 By similarity
Disulfide bondi253 ↔ 285 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO04138.

Expressioni

Tissue specificityi

Expressed at low levels in leaves, sheaths and meristems.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO04138.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 6235Chitin-binding type-1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3979.
HOGENOMiHOG000231411.
KOiK01183.
OMAiNEINGAN.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O04138-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAKMATMVA LVFGLALLLS AAAPAAAQNC GCQDGYCCSQ WGYCGTTEAY    50
CGQGCQSGPC WGSGGEAAAG MAGRKAGAGA GVSVESVVTE AFFNGIKNQA 100
PNGCAGKSFY TRQSFLNAAR SYSGFANDRT NDDSKREIAA FFAHVTHETG 150
HMCYINEING ANMDYCDKSN KQWPCQPGKK YYGRGPLQIS WNFNYGPAGK 200
NIGFDGLRDP DKVAQDPTIS FKTALWFWMN NVHQVMSQGF GATIRAINGA 250
LECNGKNPGA VNARVNYYKD YCRQFGVSPG GNLYC 285

Note: Constructed according to the conserved gene model. No experimental confirmation available.

Length:285
Mass (Da):30,451
Last modified:September 22, 2009 - v2
Checksum:i289A6A44CB5EBFCE
GO
Isoform 2 (identifier: O04138-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     57-80: SGPCWGSGGEAAAGMAGRKAGAGA → MANSPTLTMLVFLAIGLSLVLSAA

Show »
Length:229
Mass (Da):25,151
Checksum:i90F7945DECDC7433
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5656Missing in isoform 2.
VSP_037998Add
BLAST
Alternative sequencei57 – 8024SGPCW…AGAGA → MANSPTLTMLVFLAIGLSLV LSAA in isoform 2.
VSP_037999Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB054687 Genomic DNA. Translation: BAB21374.1.
AB003194 mRNA. Translation: BAA19793.1.
AL606629 Genomic DNA. Translation: CAD41540.2.
AP008210 Genomic DNA. Translation: BAF15098.1.
CM000141 Genomic DNA. Translation: EAZ31206.1.
AK060363 mRNA. Translation: BAG87422.1.
AK099973 mRNA. Translation: BAG94383.1.
PIRiT03405.
RefSeqiNP_001053184.1. NM_001059719.1. [O04138-2]
UniGeneiOs.95634.

Genome annotation databases

GeneIDi4336263.
KEGGiosa:4336263.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB054687 Genomic DNA. Translation: BAB21374.1 .
AB003194 mRNA. Translation: BAA19793.1 .
AL606629 Genomic DNA. Translation: CAD41540.2 .
AP008210 Genomic DNA. Translation: BAF15098.1 .
CM000141 Genomic DNA. Translation: EAZ31206.1 .
AK060363 mRNA. Translation: BAG87422.1 .
AK099973 mRNA. Translation: BAG94383.1 .
PIRi T03405.
RefSeqi NP_001053184.1. NM_001059719.1. [O04138-2 ]
UniGenei Os.95634.

3D structure databases

ProteinModelPortali O04138.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbi O04138.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4336263.
KEGGi osa:4336263.

Organism-specific databases

Gramenei O04138.

Phylogenomic databases

eggNOGi COG3979.
HOGENOMi HOG000231411.
KOi K01183.
OMAi NEINGAN.

Family and domain databases

Gene3Di 3.30.60.10. 1 hit.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view ]
PIRSFi PIRSF001060. Endochitinase. 1 hit.
ProDomi PD000609. Chitin_bd_1. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence and polymorphisms of a rice chitinase gene, Cht4."
    Nakazaki T., Ikehashi H.
    Breed. Sci. 48:371-376(1998)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases."
    Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y.
    Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  6. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  7. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Nipponbare.
  8. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
    Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
    Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHI4_ORYSJ
AccessioniPrimary (citable) accession number: O04138
Secondary accession number(s): Q7XU64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: May 14, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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