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O04138

- CHI4_ORYSJ

UniProt

O04138 - CHI4_ORYSJ

Protein

Chitinase 4

Gene

Cht4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Hydrolyzes chitin and may function in reproductive organs during embryogenesis and seed maturation.1 Publication

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    GO - Molecular functioni

    1. chitinase activity Source: UniProtKB
    2. chitin binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. chitin catabolic process Source: UniProtKB-KW
    3. defense response Source: UniProtKB-KW
    4. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

    Keywords - Ligandi

    Chitin-binding

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.
    GH19. Glycoside Hydrolase Family 19.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chitinase 4 (EC:3.2.1.14)
    Alternative name(s):
    OsChia2b
    Pathogenesis related (PR)-3 chitinase 4
    Gene namesi
    Name:Cht4
    Ordered Locus Names:Os04g0493400, LOC_Os04g41620
    ORF Names:OsJ_15306, OSJNBb0091E11.8
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 4

    Organism-specific databases

    GrameneiO04138.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 285258Chitinase 4PRO_0000383463Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 38PROSITE-ProRule annotation
    Disulfide bondi32 ↔ 44PROSITE-ProRule annotation
    Disulfide bondi37 ↔ 51PROSITE-ProRule annotation
    Disulfide bondi55 ↔ 60PROSITE-ProRule annotation
    Disulfide bondi104 ↔ 153PROSITE-ProRule annotation
    Disulfide bondi166 ↔ 175PROSITE-ProRule annotation
    Disulfide bondi253 ↔ 285PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiO04138.

    Expressioni

    Tissue specificityi

    Expressed at low levels in leaves, sheaths and meristems.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO04138.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 6235Chitin-binding type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3979.
    HOGENOMiHOG000231411.
    KOiK01183.
    OMAiNEINGAN.

    Family and domain databases

    Gene3Di3.30.60.10. 1 hit.
    InterProiIPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view]
    PfamiPF00187. Chitin_bind_1. 1 hit.
    PF00182. Glyco_hydro_19. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001060. Endochitinase. 1 hit.
    ProDomiPD000609. Chitin_bd_1. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00270. ChtBD1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O04138-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAKMATMVA LVFGLALLLS AAAPAAAQNC GCQDGYCCSQ WGYCGTTEAY    50
    CGQGCQSGPC WGSGGEAAAG MAGRKAGAGA GVSVESVVTE AFFNGIKNQA 100
    PNGCAGKSFY TRQSFLNAAR SYSGFANDRT NDDSKREIAA FFAHVTHETG 150
    HMCYINEING ANMDYCDKSN KQWPCQPGKK YYGRGPLQIS WNFNYGPAGK 200
    NIGFDGLRDP DKVAQDPTIS FKTALWFWMN NVHQVMSQGF GATIRAINGA 250
    LECNGKNPGA VNARVNYYKD YCRQFGVSPG GNLYC 285

    Note: Constructed according to the conserved gene model. No experimental confirmation available.

    Length:285
    Mass (Da):30,451
    Last modified:September 22, 2009 - v2
    Checksum:i289A6A44CB5EBFCE
    GO
    Isoform 2 (identifier: O04138-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-56: Missing.
         57-80: SGPCWGSGGEAAAGMAGRKAGAGA → MANSPTLTMLVFLAIGLSLVLSAA

    Show »
    Length:229
    Mass (Da):25,151
    Checksum:i90F7945DECDC7433
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5656Missing in isoform 2. 2 PublicationsVSP_037998Add
    BLAST
    Alternative sequencei57 – 8024SGPCW…AGAGA → MANSPTLTMLVFLAIGLSLV LSAA in isoform 2. 2 PublicationsVSP_037999Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB054687 Genomic DNA. Translation: BAB21374.1.
    AB003194 mRNA. Translation: BAA19793.1.
    AL606629 Genomic DNA. Translation: CAD41540.2.
    AP008210 Genomic DNA. Translation: BAF15098.1.
    CM000141 Genomic DNA. Translation: EAZ31206.1.
    AK060363 mRNA. Translation: BAG87422.1.
    AK099973 mRNA. Translation: BAG94383.1.
    PIRiT03405.
    RefSeqiNP_001053184.1. NM_001059719.1. [O04138-2]
    UniGeneiOs.95634.

    Genome annotation databases

    GeneIDi4336263.
    KEGGiosa:4336263.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB054687 Genomic DNA. Translation: BAB21374.1 .
    AB003194 mRNA. Translation: BAA19793.1 .
    AL606629 Genomic DNA. Translation: CAD41540.2 .
    AP008210 Genomic DNA. Translation: BAF15098.1 .
    CM000141 Genomic DNA. Translation: EAZ31206.1 .
    AK060363 mRNA. Translation: BAG87422.1 .
    AK099973 mRNA. Translation: BAG94383.1 .
    PIRi T03405.
    RefSeqi NP_001053184.1. NM_001059719.1. [O04138-2 ]
    UniGenei Os.95634.

    3D structure databases

    ProteinModelPortali O04138.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM18. Carbohydrate-Binding Module Family 18.
    GH19. Glycoside Hydrolase Family 19.

    Proteomic databases

    PaxDbi O04138.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4336263.
    KEGGi osa:4336263.

    Organism-specific databases

    Gramenei O04138.

    Phylogenomic databases

    eggNOGi COG3979.
    HOGENOMi HOG000231411.
    KOi K01183.
    OMAi NEINGAN.

    Family and domain databases

    Gene3Di 3.30.60.10. 1 hit.
    InterProi IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view ]
    Pfami PF00187. Chitin_bind_1. 1 hit.
    PF00182. Glyco_hydro_19. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001060. Endochitinase. 1 hit.
    ProDomi PD000609. Chitin_bd_1. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00270. ChtBD1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence and polymorphisms of a rice chitinase gene, Cht4."
      Nakazaki T., Ikehashi H.
      Breed. Sci. 48:371-376(1998)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases."
      Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y.
      Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    6. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    7. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Nipponbare.
    8. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
      Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
      Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCHI4_ORYSJ
    AccessioniPrimary (citable) accession number: O04138
    Secondary accession number(s): Q7XU64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3