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Protein

Chitinase 4

Gene

Cht4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes chitin and may function in reproductive organs during embryogenesis and seed maturation.1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  • chitinase activity Source: UniProtKB
  • chitin binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 4 (EC:3.2.1.14)
Alternative name(s):
OsChia2b
Pathogenesis related (PR)-3 chitinase 4
Gene namesi
Name:Cht4
Ordered Locus Names:Os04g0493400, LOC_Os04g41620
ORF Names:OsJ_15306, OSJNBb0091E11.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 4

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 285258Chitinase 4PRO_0000383463Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 38PROSITE-ProRule annotation
Disulfide bondi32 ↔ 44PROSITE-ProRule annotation
Disulfide bondi37 ↔ 51PROSITE-ProRule annotation
Disulfide bondi55 ↔ 60PROSITE-ProRule annotation
Disulfide bondi104 ↔ 153PROSITE-ProRule annotation
Disulfide bondi166 ↔ 175PROSITE-ProRule annotation
Disulfide bondi253 ↔ 285PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO04138.

Expressioni

Tissue specificityi

Expressed at low levels in leaves, sheaths and meristems.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os04g41620.1.

Structurei

3D structure databases

ProteinModelPortaliO04138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 6235Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiO04138.
OMAiYCQSSNT.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 2 hits.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O04138-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAKMATMVA LVFGLALLLS AAAPAAAQNC GCQDGYCCSQ WGYCGTTEAY
60 70 80 90 100
CGQGCQSGPC WGSGGEAAAG MAGRKAGAGA GVSVESVVTE AFFNGIKNQA
110 120 130 140 150
PNGCAGKSFY TRQSFLNAAR SYSGFANDRT NDDSKREIAA FFAHVTHETG
160 170 180 190 200
HMCYINEING ANMDYCDKSN KQWPCQPGKK YYGRGPLQIS WNFNYGPAGK
210 220 230 240 250
NIGFDGLRDP DKVAQDPTIS FKTALWFWMN NVHQVMSQGF GATIRAINGA
260 270 280
LECNGKNPGA VNARVNYYKD YCRQFGVSPG GNLYC
Note: Constructed according to the conserved gene model. No experimental confirmation available.
Length:285
Mass (Da):30,451
Last modified:September 22, 2009 - v2
Checksum:i289A6A44CB5EBFCE
GO
Isoform 2 (identifier: O04138-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     57-80: SGPCWGSGGEAAAGMAGRKAGAGA → MANSPTLTMLVFLAIGLSLVLSAA

Show »
Length:229
Mass (Da):25,151
Checksum:i90F7945DECDC7433
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5656Missing in isoform 2. 2 PublicationsVSP_037998Add
BLAST
Alternative sequencei57 – 8024SGPCW…AGAGA → MANSPTLTMLVFLAIGLSLV LSAA in isoform 2. 2 PublicationsVSP_037999Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054687 Genomic DNA. Translation: BAB21374.1.
AB003194 mRNA. Translation: BAA19793.1.
AL606629 Genomic DNA. Translation: CAD41540.2.
AP008210 Genomic DNA. Translation: BAF15098.1.
AP014960 Genomic DNA. Translation: BAS89866.1.
CM000141 Genomic DNA. Translation: EAZ31206.1.
AK060363 mRNA. Translation: BAG87422.1.
AK099973 mRNA. Translation: BAG94383.1.
PIRiT03405.
UniGeneiOs.28044.
Os.95634.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB054687 Genomic DNA. Translation: BAB21374.1.
AB003194 mRNA. Translation: BAA19793.1.
AL606629 Genomic DNA. Translation: CAD41540.2.
AP008210 Genomic DNA. Translation: BAF15098.1.
AP014960 Genomic DNA. Translation: BAS89866.1.
CM000141 Genomic DNA. Translation: EAZ31206.1.
AK060363 mRNA. Translation: BAG87422.1.
AK099973 mRNA. Translation: BAG94383.1.
PIRiT03405.
UniGeneiOs.28044.
Os.95634.

3D structure databases

ProteinModelPortaliO04138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os04g41620.1.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbiO04138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiO04138.
OMAiYCQSSNT.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 2 hits.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHI4_ORYSJ
AccessioniPrimary (citable) accession number: O04138
Secondary accession number(s): Q7XU64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: September 7, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.