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O04138 (CHI4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chitinase 4

EC=3.2.1.14
Alternative name(s):
OsChia2b
Pathogenesis related (PR)-3 chitinase 4
Gene names
Name:Cht4
Ordered Locus Names:Os04g0493400, LOC_Os04g41620
ORF Names:OsJ_15306, OSJNBb0091E11.8
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes chitin and may function in reproductive organs during embryogenesis and seed maturation. Ref.2

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Tissue specificity

Expressed at low levels in leaves, sheaths and meristems. Ref.8

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class IV subfamily.

Contains 1 chitin-binding type-1 domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O04138-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Constructed according to the conserved gene model. No experimental confirmation available.
Isoform 2 (identifier: O04138-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.
     57-80: SGPCWGSGGEAAAGMAGRKAGAGA → MANSPTLTMLVFLAIGLSLVLSAA

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 285258Chitinase 4
PRO_0000383463

Regions

Domain28 – 6235Chitin-binding type-1

Amino acid modifications

Disulfide bond30 ↔ 38 By similarity
Disulfide bond32 ↔ 44 By similarity
Disulfide bond37 ↔ 51 By similarity
Disulfide bond55 ↔ 60 By similarity
Disulfide bond104 ↔ 153 By similarity
Disulfide bond166 ↔ 175 By similarity
Disulfide bond253 ↔ 285 By similarity

Natural variations

Alternative sequence1 – 5656Missing in isoform 2.
VSP_037998
Alternative sequence57 – 8024SGPCW…AGAGA → MANSPTLTMLVFLAIGLSLV LSAA in isoform 2.
VSP_037999

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 289A6A44CB5EBFCE

FASTA28530,451
        10         20         30         40         50         60 
MAAKMATMVA LVFGLALLLS AAAPAAAQNC GCQDGYCCSQ WGYCGTTEAY CGQGCQSGPC 

        70         80         90        100        110        120 
WGSGGEAAAG MAGRKAGAGA GVSVESVVTE AFFNGIKNQA PNGCAGKSFY TRQSFLNAAR 

       130        140        150        160        170        180 
SYSGFANDRT NDDSKREIAA FFAHVTHETG HMCYINEING ANMDYCDKSN KQWPCQPGKK 

       190        200        210        220        230        240 
YYGRGPLQIS WNFNYGPAGK NIGFDGLRDP DKVAQDPTIS FKTALWFWMN NVHQVMSQGF 

       250        260        270        280 
GATIRAINGA LECNGKNPGA VNARVNYYKD YCRQFGVSPG GNLYC 

« Hide

Isoform 2 [UniParc].

Checksum: 90F7945DECDC7433
Show »

FASTA22925,151

References

« Hide 'large scale' references
[1]"Genomic sequence and polymorphisms of a rice chitinase gene, Cht4."
Nakazaki T., Ikehashi H.
Breed. Sci. 48:371-376(1998)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases."
Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y.
Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION.
Strain: cv. Nipponbare.
[3]"Sequence and analysis of rice chromosome 4."
Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. expand/collapse author list , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B.
Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[6]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[7]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Nipponbare.
[8]"Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB054687 Genomic DNA. Translation: BAB21374.1.
AB003194 mRNA. Translation: BAA19793.1.
AL606629 Genomic DNA. Translation: CAD41540.2.
AP008210 Genomic DNA. Translation: BAF15098.1.
CM000141 Genomic DNA. Translation: EAZ31206.1.
AK060363 mRNA. Translation: BAG87422.1.
AK099973 mRNA. Translation: BAG94383.1.
PIRT03405.
RefSeqNP_001053184.1. NM_001059719.1.
UniGeneOs.95634.

3D structure databases

ProteinModelPortalO04138.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbO04138.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4336263.
KEGGosa:4336263.

Organism-specific databases

GrameneO04138.

Phylogenomic databases

eggNOGCOG3979.
HOGENOMHOG000231411.
KOK01183.
OMANEINGAN.

Family and domain databases

Gene3D3.30.60.10. 1 hit.
InterProIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
ProDomPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHI4_ORYSJ
AccessionPrimary (citable) accession number: O04138
Secondary accession number(s): Q7XU64
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: April 16, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries