Reviewed, UniProtKB/Swiss-Prot O04130 (SERA_ARATH)

Names and origin

Protein names

Recommended name:
    D-3-phosphoglycerate dehydrogenase, chloroplastic
      Short name=3-PGDH
    EC=1.1.1.95

Gene names

Ordered Locus Names:	At1g17745
ORF Names:	F11A6.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR cytosol Ref.5 Inferred from direct assay. Source: TAIR nucleus Ref.5 Inferred from direct assay. Source: TAIR
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 624	575	D-3-phosphoglycerate dehydrogenase, chloroplastic		PRO_0000007192
Regions
	Domain	551 – 621	71	ACT
Sites
	Active site	312	1	By similarity
	Active site	341	1	By similarity
	Active site	360	1	Proton donor By similarity
Amino acid modifications
	Modified residue	71	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O04130-1 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: 724370F870DEA310	FASTA	624	66,454
	10 20 30 40 50 60 MAFSSSCSSV KAVNSRWTSP SPSPSSRFAV LPAFLHRRYA TSVKLTAISA ALKTVEQTTL 70 80 90 100 110 120 TEDNRFSTVG SDSDEYNPTL PKPRILVTEK LGEAGVNLLR EFGDVDCSYD LSPEDLKKKV 130 140 150 160 170 180 AESDALIVRS GTKVTREVFE AAKGRLKVVG RAGVGIDNVD LQAATEHGCL VVNAPTANTV 190 200 210 220 230 240 AAAEHGIALL ASMARNVAQA DASIKAGKWE RSKYVGVSLV GKTLAVMGFG KVGTEVARRA 250 260 270 280 290 300 KGLGMTVISH DPYAPADRAR ALGVDLVSFD QAISTADFVS LHMPLTPATK KVFNDETFSK 310 320 330 340 350 360 MKKGVRLINV ARGGVIDEDA LVRALDAGIV AQAALDVFCE EPPSKDSRLI QHENVTVTPH 370 380 390 400 410 420 LGASTKEAQE GVAIEIAEAV AGALKGELSA TAVNAPMVAP EVLSELTPYI VLAEKLGRLA 430 440 450 460 470 480 VQLASGGKGV QSIRVVYRSA RDRDDLDTRL LRAMITKGII EPISDSYVNL VNADFIAKQK 490 500 510 520 530 540 GLRISEERMV VDSSPEYPVD SIQVQILNVE SNFAGAVSDA GDISIEGKVK YGVPHLTCVG 550 560 570 580 590 600 SFGVDVSLEG NLILCRQVDQ PGMIGQVGNI LGEQNVNVNF MSVGRTVLRK QAIMAIGVDE 610 620 EPDNKTLERI GGVSAIEEFV FLKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of serine biosynthesis in Arabidopsis. Crucial role of plastidic 3-phosphoglycerate dehydrogenase in non-photosynthetic tissues." Ho C.-L., Noji M., Saito M., Saito K. J. Biol. Chem. 274:397-402(1999) [PubMed: 9867856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed: 18433157] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003280 mRNA. Translation: BAA20405.1. AB010407 Genomic DNA. Translation: BAA24440.1. AC034257 Genomic DNA. Translation: AAF99816.1. AY050399 mRNA. Translation: AAK91415.1. AY098953 mRNA. Translation: AAM19963.1. AY086001 mRNA. Translation: AAM63210.1.
IPI	IPI00547060.
PIR	T52296.
RefSeq	NP_564034.1.
UniGene	At.21252 At.73011
3D structure databases
HSSP	HSSP built from PDB template 1PSD based on UniProtKB P08328.
ModBase	Search...
Proteomic databases
PRIDE	O04130.
ProMEX	O04130.
Genome annotation databases
GeneID	838352.
GenomeReviews	Gene locus AT1G17745 in contig CT485782_GR.
KEGG	ath:AT1G17745.
NMPDR	fig|3702.1.peg.2102.
Organism-specific databases
TAIR	At1g17745.
Phylogenomic databases
OMA	O04130. KSFKGME.
Enzyme and pathway databases
BRENDA	1.1.1.95. 302.
Gene expression databases
GermOnline	AT1G17745. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR006236. D-3-Phosphoglycerate_DH. IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR015508. D3PG_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10996:SF20. D3PG_Deh. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF01842. ACT. 1 hit. [Graphical view]
TIGRFAMs	TIGR01327. PGDH. 1 hit.
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_ARATH

Accession

Primary (citable) accession number: O04130

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)