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Protein

Choline monooxygenase, chloroplastic

Gene

CMO

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first step of the osmoprotectant glycine betaine synthesis.

Catalytic activityi

Choline + O2 + 2 reduced ferredoxin + 2 H+ = betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine aldehyde from choline (monooxygenase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Choline monooxygenase, chloroplastic (CMO)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine aldehyde from choline (monooxygenase route), the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi162Iron-sulfur (2Fe-2S)Curated1
Metal bindingi164Iron-sulfur (2Fe-2S); via pros nitrogenCurated1
Metal bindingi181Iron-sulfur (2Fe-2S)Curated1
Metal bindingi184Iron-sulfur (2Fe-2S); via pros nitrogenCurated1
Metal bindingi287IronSequence analysis1
Metal bindingi292IronSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3203.
BRENDAi1.14.15.7. 5812.
UniPathwayiUPA00529; UER00430.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline monooxygenase, chloroplastic (EC:1.14.15.7)
Gene namesi
Name:CMO
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 60Chloroplast1 PublicationAdd BLAST60
ChainiPRO_000002092861 – 439Choline monooxygenase, chloroplasticAdd BLAST379

Proteomic databases

PRIDEiO04121.

Expressioni

Tissue specificityi

Expressed in leaves.

Inductioni

By salt stress.

Interactioni

Subunit structurei

Homotrimer or homodimer.Curated

Structurei

3D structure databases

ProteinModelPortaliO04121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 227RieskePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the choline monooxygenase family.Curated
Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK00499.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 2 hits.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAASASATT MLLKYPTTVC GIPNPSSNNN NDPSNNIVSI PQNTTNPTLK
60 70 80 90 100
SRTPNKITTN AVAAPSFPSL TTTTPSSIQS LVHEFDPQIP PEDAHTPPSS
110 120 130 140 150
WYTEPAFYSH ELERIFYKGW QVAGISDQIK EPNQYFTGSL GNVEYLVSRD
160 170 180 190 200
GEGKVHAFHN VCTHRASILA CGSGKKSCFV CPYHGWVYGM DGSLAKASKA
210 220 230 240 250
KPEQNLDPKE LGLVPLKVAV WGPFVLISLD RSLEEGGDVG TEWLGTSAED
260 270 280 290 300
VKAHAFDPSL QFIHRSEFPM ESNWKIFSDN YLDSSYHVPY AHKYYATELN
310 320 330 340 350
FDTYDTQMIE NVTIQRVEGS SNKPDGFDRV GIQAFYAFAY PNFAVERYGP
360 370 380 390 400
WMTTMHIHPL GPRKCKLVVD YYIENSMLDD KDYIEKGIAI NDNVQREDVV
410 420 430
LCESVQRGLE TPAYRSGRYV MPIEKGIHHF HCWLQQTLK
Length:439
Mass (Da):49,214
Last modified:July 1, 1997 - v1
Checksum:i3A99FF5B14BC8FDA
GO

Mass spectrometryi

Molecular mass is 42864±22 Da from positions 61 - 439. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85780 mRNA. Translation: AAB52509.1.
PIRiT09214.

Genome annotation databases

KEGGiag:AAB52509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85780 mRNA. Translation: AAB52509.1.
PIRiT09214.

3D structure databases

ProteinModelPortaliO04121.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO04121.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAB52509.

Phylogenomic databases

KOiK00499.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00430.
BioCyciMetaCyc:MONOMER-3203.
BRENDAi1.14.15.7. 5812.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 2 hits.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHMO_SPIOL
AccessioniPrimary (citable) accession number: O04121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.