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O04121

- CHMO_SPIOL

UniProt

O04121 - CHMO_SPIOL

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Protein
Choline monooxygenase, chloroplastic
Gene
CMO
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the first step of the osmoprotectant glycine betaine synthesis.

Catalytic activityi

Choline + O2 + 2 reduced ferredoxin + 2 H+ = betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin.

Cofactori

Binds 1 2Fe-2S cluster.
Binds 1 iron ion Inferred.
Magnesium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi162 – 1621Iron-sulfur (2Fe-2S) Inferred
Metal bindingi164 – 1641Iron-sulfur (2Fe-2S); via pros nitrogen Inferred
Metal bindingi181 – 1811Iron-sulfur (2Fe-2S) Inferred
Metal bindingi184 – 1841Iron-sulfur (2Fe-2S); via pros nitrogen Inferred
Metal bindingi287 – 2871Iron Reviewed prediction
Metal bindingi292 – 2921Iron Reviewed prediction

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. choline monooxygenase activity Source: UniProtKB-EC
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of two atoms of oxygen into one donor Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. aromatic compound catabolic process Source: InterPro
  2. glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway
  3. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00529; UER00430.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline monooxygenase, chloroplastic (EC:1.14.15.7)
Gene namesi
Name:CMO
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

  1. chloroplast stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6060Chloroplast1 Publication
Add
BLAST
Chaini61 – 439379Choline monooxygenase, chloroplastic
PRO_0000020928Add
BLAST

Expressioni

Tissue specificityi

Expressed in leaves.

Inductioni

By salt stress.

Interactioni

Subunit structurei

Homotrimer or homodimer Inferred.

Structurei

3D structure databases

ProteinModelPortaliO04121.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini120 – 227108Rieske
Add
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 2 hits.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O04121-1 [UniParc]FASTAAdd to Basket

« Hide

MMAASASATT MLLKYPTTVC GIPNPSSNNN NDPSNNIVSI PQNTTNPTLK    50
SRTPNKITTN AVAAPSFPSL TTTTPSSIQS LVHEFDPQIP PEDAHTPPSS 100
WYTEPAFYSH ELERIFYKGW QVAGISDQIK EPNQYFTGSL GNVEYLVSRD 150
GEGKVHAFHN VCTHRASILA CGSGKKSCFV CPYHGWVYGM DGSLAKASKA 200
KPEQNLDPKE LGLVPLKVAV WGPFVLISLD RSLEEGGDVG TEWLGTSAED 250
VKAHAFDPSL QFIHRSEFPM ESNWKIFSDN YLDSSYHVPY AHKYYATELN 300
FDTYDTQMIE NVTIQRVEGS SNKPDGFDRV GIQAFYAFAY PNFAVERYGP 350
WMTTMHIHPL GPRKCKLVVD YYIENSMLDD KDYIEKGIAI NDNVQREDVV 400
LCESVQRGLE TPAYRSGRYV MPIEKGIHHF HCWLQQTLK 439
Length:439
Mass (Da):49,214
Last modified:July 1, 1997 - v1
Checksum:i3A99FF5B14BC8FDA
GO

Mass spectrometryi

Molecular mass is 42864±22 Da from positions 61 - 439. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85780 mRNA. Translation: AAB52509.1.
PIRiT09214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85780 mRNA. Translation: AAB52509.1 .
PIRi T09214.

3D structure databases

ProteinModelPortali O04121.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00529 ; UER00430 .

Family and domain databases

Gene3Di 2.102.10.10. 1 hit.
3.90.380.10. 2 hits.
InterProi IPR017941. Rieske_2Fe-2S.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view ]
Pfami PF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view ]
PRINTSi PR00090. RNGDIOXGNASE.
SUPFAMi SSF50022. SSF50022. 1 hit.
PROSITEi PS51296. RIESKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Choline monooxygenase, an unusual iron-sulfur enzyme catalyzing the first step of glycine betaine synthesis in plants: prosthetic group characterization and cDNA cloning."
    Rathinasabapathi B., Burnet M., Russell B.L., Gage D.A., Liao P.-C., Nye G.J., Scott P., Golbeck J.H., Hanson A.D.
    Proc. Natl. Acad. Sci. U.S.A. 94:3454-3458(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 61-84; 200-225; 253-288; 317-361; 367-379; 408-415 AND 419-438, MASS SPECTROMETRY.
    Strain: cv. Savoy hybrid 612.
    Tissue: Leaf.

Entry informationi

Entry nameiCHMO_SPIOL
AccessioniPrimary (citable) accession number: O04121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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