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O04121

- CHMO_SPIOL

UniProt

O04121 - CHMO_SPIOL

Protein

Choline monooxygenase, chloroplastic

Gene

CMO

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the first step of the osmoprotectant glycine betaine synthesis.

    Catalytic activityi

    Choline + O2 + 2 reduced ferredoxin + 2 H+ = betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin.

    Cofactori

    Binds 1 2Fe-2S cluster.
    Binds 1 iron ion.Curated
    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi162 – 1621Iron-sulfur (2Fe-2S)Curated
    Metal bindingi164 – 1641Iron-sulfur (2Fe-2S); via pros nitrogenCurated
    Metal bindingi181 – 1811Iron-sulfur (2Fe-2S)Curated
    Metal bindingi184 – 1841Iron-sulfur (2Fe-2S); via pros nitrogenCurated
    Metal bindingi287 – 2871IronSequence Analysis
    Metal bindingi292 – 2921IronSequence Analysis

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. choline monooxygenase activity Source: UniProtKB-EC
    3. iron ion binding Source: InterPro
    4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of two atoms of oxygen into one donor Source: InterPro

    GO - Biological processi

    1. aromatic compound catabolic process Source: InterPro
    2. glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway
    3. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00529; UER00430.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline monooxygenase, chloroplastic (EC:1.14.15.7)
    Gene namesi
    Name:CMO
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6060Chloroplast1 PublicationAdd
    BLAST
    Chaini61 – 439379Choline monooxygenase, chloroplasticPRO_0000020928Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in leaves.

    Inductioni

    By salt stress.

    Interactioni

    Subunit structurei

    Homotrimer or homodimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliO04121.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini120 – 227108RieskePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the choline monooxygenase family.Curated
    Contains 1 Rieske domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.102.10.10. 1 hit.
    3.90.380.10. 2 hits.
    InterProiIPR017941. Rieske_2Fe-2S.
    IPR015879. Ring_hydroxy_dOase_asu_C_dom.
    IPR001663. Rng_hydr_dOase-A.
    [Graphical view]
    PfamiPF00355. Rieske. 1 hit.
    PF00848. Ring_hydroxyl_A. 1 hit.
    [Graphical view]
    PRINTSiPR00090. RNGDIOXGNASE.
    SUPFAMiSSF50022. SSF50022. 1 hit.
    PROSITEiPS51296. RIESKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O04121-1 [UniParc]FASTAAdd to Basket

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    MMAASASATT MLLKYPTTVC GIPNPSSNNN NDPSNNIVSI PQNTTNPTLK    50
    SRTPNKITTN AVAAPSFPSL TTTTPSSIQS LVHEFDPQIP PEDAHTPPSS 100
    WYTEPAFYSH ELERIFYKGW QVAGISDQIK EPNQYFTGSL GNVEYLVSRD 150
    GEGKVHAFHN VCTHRASILA CGSGKKSCFV CPYHGWVYGM DGSLAKASKA 200
    KPEQNLDPKE LGLVPLKVAV WGPFVLISLD RSLEEGGDVG TEWLGTSAED 250
    VKAHAFDPSL QFIHRSEFPM ESNWKIFSDN YLDSSYHVPY AHKYYATELN 300
    FDTYDTQMIE NVTIQRVEGS SNKPDGFDRV GIQAFYAFAY PNFAVERYGP 350
    WMTTMHIHPL GPRKCKLVVD YYIENSMLDD KDYIEKGIAI NDNVQREDVV 400
    LCESVQRGLE TPAYRSGRYV MPIEKGIHHF HCWLQQTLK 439
    Length:439
    Mass (Da):49,214
    Last modified:July 1, 1997 - v1
    Checksum:i3A99FF5B14BC8FDA
    GO

    Mass spectrometryi

    Molecular mass is 42864±22 Da from positions 61 - 439. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U85780 mRNA. Translation: AAB52509.1.
    PIRiT09214.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U85780 mRNA. Translation: AAB52509.1 .
    PIRi T09214.

    3D structure databases

    ProteinModelPortali O04121.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00529 ; UER00430 .

    Family and domain databases

    Gene3Di 2.102.10.10. 1 hit.
    3.90.380.10. 2 hits.
    InterProi IPR017941. Rieske_2Fe-2S.
    IPR015879. Ring_hydroxy_dOase_asu_C_dom.
    IPR001663. Rng_hydr_dOase-A.
    [Graphical view ]
    Pfami PF00355. Rieske. 1 hit.
    PF00848. Ring_hydroxyl_A. 1 hit.
    [Graphical view ]
    PRINTSi PR00090. RNGDIOXGNASE.
    SUPFAMi SSF50022. SSF50022. 1 hit.
    PROSITEi PS51296. RIESKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Choline monooxygenase, an unusual iron-sulfur enzyme catalyzing the first step of glycine betaine synthesis in plants: prosthetic group characterization and cDNA cloning."
      Rathinasabapathi B., Burnet M., Russell B.L., Gage D.A., Liao P.-C., Nye G.J., Scott P., Golbeck J.H., Hanson A.D.
      Proc. Natl. Acad. Sci. U.S.A. 94:3454-3458(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 61-84; 200-225; 253-288; 317-361; 367-379; 408-415 AND 419-438, MASS SPECTROMETRY.
      Strain: cv. Savoy hybrid 612.
      Tissue: Leaf.

    Entry informationi

    Entry nameiCHMO_SPIOL
    AccessioniPrimary (citable) accession number: O04121
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2002
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3