O04104 (URIC2_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Uricase-2 isozyme 2 EC=1.7.3.3 Alternative name(s): Nodulin 35 Short name=N-35 Non-symbiotic uricase Urate oxidase Uricase II isozyme 2
Organism	Glycine max (Soybean) (Glycine hispida)
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2.
Pathway	Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.
Subunit structure	Homotetramer By similarity.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the uricase family.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Peroxisome
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	urate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Uricase-2 isozyme 2		PRO_0000166004
Regions
	Region	64 – 65	2	Substrate binding By similarity
	Region	238 – 239	2	Substrate binding By similarity
Sites
	Active site	183	1	Charge relay system By similarity
	Active site	239	1	Charge relay system By similarity
	Binding site	183	1	Substrate By similarity
Experimental info
	Sequence conflict	208	1	L → F in AAA33994. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04104 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 81B8F8F7ACAA00A9	FASTA	309	35,137
	10 20 30 40 50 60 MAQQEVVEGF KFEQRHGKER VRVARVWKTR QGQHFVVEWR VGITLFSDCV NSYLRDDNSD 70 80 90 100 110 120 IVATDTMKNT VYAKAKECSD ILSAEDFAIL LAKHFVSFYK KVTGAIVNIV EKPWERVIVD 130 140 150 160 170 180 GQPHEHGFKL GSEKHTTEAI VQKSGSLQLT SGIEGLSVLK TTQSGFVNFI RDKYTALPDT 190 200 210 220 230 240 RERILATEVT ALWRYSYESQ YSLPQKPLYF TEKYQEVKKV LADTFFGPPN GGVYSPSVQN 250 260 270 280 290 300 TLYLMAKATL NRFPDIAYVS LKMPNLHFLP VNISNKDGPI VKFEDDVYLP TDEPHGSIQA SLSRLWSKL

References

[1]	"Primary structure of the soybean nodulin-35 gene encoding uricase II localized in the peroxisome of uninfected cells of nodules." Nguyen T., Zelechowska M., Foster V., Bergmann H., Verma D.P.S. Proc. Natl. Acad. Sci. U.S.A. 82:5040-5044(1985) [PubMed: 16593585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 245-262. Strain: cv. Dare.
[2]	"Two distinct uricase II (nodulin 35) genes are differentially expressed in soybean plants." Takane K., Tajima S., Kouchi H. Mol. Plant Microbe Interact. 10:735-741(1997) [PubMed: 9245835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Akisengoku. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10594 Genomic DNA. Translation: AAA33994.1. AB002807 Genomic DNA. Translation: BAA19670.1. AB002809 mRNA. Translation: BAA19672.1.
RefSeq	NP_001235948.1. NM_001249019.1.
UniGene	Gma.29861.
3D structure databases
ProteinModelPortal	O04104.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100037445.
Gene expression databases
Genevestigator	O04104.
Family and domain databases
InterPro	IPR002042. Uricase. IPR019842. Uricase_CS. [Graphical view]
Gene3D	G3DSA:3.10.270.10. Uricase. 1 hit.
PANTHER	PTHR10395:SF1. Uricase. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PIRSF	PIRSF000241. Urate_oxidase. 1 hit.
PRINTS	PR00093. URICASE.
TIGRFAMs	TIGR03383. Urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC2_SOYBN

Accession

Primary (citable) accession number: O04104

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program