Reviewed, UniProtKB/Swiss-Prot O04046 (GGPP2_ARATH)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl pyrophosphate synthetase 2
      Short name=GGPP synthetase 2
      Short name=GGPS2
Including the following 3 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10
    3- Recommended name:
            Farnesyltranstransferase
              EC=2.5.1.29

Gene names

Name:	GGPP2
Synonyms:	GGPP5
Ordered Locus Names:	At2g23800
ORF Names:	F27L4.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Pathway	Isoprenoid biosynthesis; farnesyl-PP biosynthesis; farnesyl-PP from geranyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranyl-PP biosynthesis; geranyl-PP from dimethylallyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranylgeranyl-PP biosynthesis; geranylgeranyl-PP from farnesyl-PP and isopentenyl-PP: step 1/1.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Tissue specificity	Mainly expressed in flowers. Ref.7
Sequence similarities	Belongs to the FPP/GGPP synthetase family.
Sequence caution	The sequence AAB67730.1 differs from that shown. Reason: Frameshift at position 5.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC farnesyltranstransferase activity Ref.6 Inferred from direct assay. Source: TAIR geranyltranstransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 376	352	Geranylgeranyl pyrophosphate synthetase 2		PRO_0000045402
Regions
	Transmembrane	188 – 208	21	Potential
	Compositional bias	60 – 72	13	Poly-Glu
Experimental info
	Sequence conflict	146 – 147	2	AM → TI in AAB67730. Ref.1
	Sequence conflict	182	1	H → D in AAB67730. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04046-1 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: 1B643EC4086BD43B	FASTA	376	41,339
	10 20 30 40 50 60 MEPQILFLYL SLFILSLNFF FTNLKPRLVR LFQPSLESRV KTALLSRKEV AAFLDSPIVE 70 80 90 100 110 120 DEEGEEREEE EEGGIVSNAN FTFEFDPYMM SKAESVNKAL EEAIPVGEPL KIHEAMRYAI 130 140 150 160 170 180 LAAGKRVRPI LCLASCELVG GQENAAMPAA CAVEMIHTMS LIKDDLPCMD NDDLRRGKPT 190 200 210 220 230 240 THKVYGEGVA ILSGGALLSL AFEHMTTAEI SSERMVWAVR ELARSIGTRG LVAGQAMDIS 250 260 270 280 290 300 SEGLDLNEVG LEHLEFIHVH KTAVLLETAA VLGAIIGGGS DEEIESVRKF ARCIGLLFQV 310 320 330 340 350 360 VDDILDETKS SEELGKTAGK DQLAGKLTYP KLIGLEKSKE FVKRLTKDAR QHLQGFSSEK 370 VAPLVALTTF IANRNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two more members of an Arabidopsis geranylgeranyl pyrophosphate synthase gene family." Scolnik P.A., Bartley G.E. Plant Gene Register PGR96-014 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta. Tissue: Flower.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Cloning and functional expression of a novel geranylgeranyl pyrophosphate synthase gene from Arabidopsis thaliana in Escherichia coli." Zhu X.F., Suzuki K., Okada K., Tanaka K., Nakagawa T., Kawamukai M., Matsuda K. Plant Cell Physiol. 38:357-361(1997) [PubMed: 9150607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-376.
[7]	"Five geranylgeranyl diphosphate synthases expressed in different organs are localized into three subcellular compartments in Arabidopsis." Okada K., Saito T., Nakagawa T., Kawamukai M., Kamiya Y. Plant Physiol. 122:1045-1056(2000) [PubMed: 10759500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-376, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U44876 mRNA. Translation: AAB67730.1. Frameshift. AC004482 Genomic DNA. Translation: AAC17083.1. BT005328 mRNA. Translation: AAO63392.1. AK117954 mRNA. Translation: BAC42592.1. AY087521 mRNA. Translation: AAM65063.1. D85029 mRNA. Translation: BAA19583.1.
IPI	IPI00535829.
PIR	S71230. T02429.
RefSeq	NP_179960.1.
UniGene	At.1550
3D structure databases
SMR	O04046. Positions 86-371.
ModBase	Search...
Proteomic databases
PRIDE	O04046.
Genome annotation databases
GeneID	816912.
GenomeReviews	Gene locus AT2G23800 in contig CT485783_GR.
KEGG	ath:AT2G23800.
NMPDR	fig|3702.1.peg.9405.
Organism-specific databases
TAIR	At2g23800.
Phylogenomic databases
OMA	O04046. ISSERMV.
Enzyme and pathway databases
BioCyc	MetaCyc:AT2G23800-MON.
BRENDA	2.5.1.1. 302. 2.5.1.10. 302. 2.5.1.29. 302.
Gene expression databases
ArrayExpress	O04046.
GermOnline	AT2G23800. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGPP2_ARATH

Accession

Primary (citable) accession number: O04046
Secondary accession number(s): Q38917, Q7DN59

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)