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O04015

- P5CS_ACTDE

UniProt

O04015 - P5CS_ACTDE

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Protein
Delta-1-pyrroline-5-carboxylate synthase
Gene
N/A
Organism
Actinidia deliciosa (Kiwi)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation

Enzyme regulationi

Feedback regulated by proline.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601Substrate By similarity
Binding sitei157 – 1571Substrate By similarity
Binding sitei176 – 1761Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi196 – 1972ATP By similarity
Nucleotide bindingi236 – 2427ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-EC
  3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
OrganismiActinidia deliciosa (Kiwi)
Taxonomic identifieri3627 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridsEricalesActinidiaceaeActinidia

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717Delta-1-pyrroline-5-carboxylate synthaseUniRule annotation
PRO_0000109774Add
BLAST

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Structurei

3D structure databases

ProteinModelPortaliO04015.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 296296Glutamate 5-kinaseUniRule annotation
Add
BLAST
Regioni297 – 717421Gamma-glutamyl phosphate reductaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA.
MF_00456. ProB.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O04015-1 [UniParc]FASTAAdd to Basket

« Hide

MDAVDSTRAF VKGVKRVIIK VGTAVVTRAD GRLALGRLGA LCEQIHELNS    50
QGFEVILVTS GAVGVGRQRL RYRKLVNSSF ADLQKPQIEL DGKACAAVGQ 100
NGLLALYDTL FSQLDVTSAQ LLVTDNDFRD PEFRKQLTET VESLLNLKVI 150
PIFNENDAVS TRKAPYEDAS GIFWDNDSLA ALLALELKAD LLVLLSDVEG 200
LYSGPPSDPQ SKLIHTYIKE MFEGLITFGD KSRVGRGGMT AKVKAAVYAA 250
HAGIPVVITS GYATNNIIKV LQGERIGTLF HRDAQKWAPV GDVGARDMAV 300
AARESSRRLQ AMSPQDRSKI LLDVADALEA NEKLIRIENE ADLAAAQQAG 350
YEKSLISRLA LKSGKISSLA KSIRVLANME EPIGHVLKRT EITDGLVLEK 400
TSSPLGVLLI IFESRPDALV QIASLAIRSG NGLVLKGGKE AKRSNAILHK 450
VITSAIPENV GPRLIGLVTS REEIPDLLKL DDVIDLVIPR GSNKLVSQIK 500
ESTKIPVLGH ADGICHVYVD KSANMDMAKK VVLDAKTDYP AACNAMETLL 550
VHKDLVQNGC LDELIVELQI KGVVIHGGPR ASSLLHIPEA RSLHHEYSSL 600
ACTIEIVDDV YAAIDHIHRH GSAHTDSIIT EDHEVAEIFL RQVDSSSVLH 650
NASTRFSDGA RFGLGAEVGI STSRIHARGP VGVEGLLTTR WIARGSGQVV 700
DGDKGIVYTH KDLTSHA 717
Length:717
Mass (Da):77,440
Last modified:July 1, 1997 - v1
Checksum:iBC76103DB08C6F8C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92286 mRNA. Translation: AAC14481.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92286 mRNA. Translation: AAC14481.1 .

3D structure databases

ProteinModelPortali O04015.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
UPA00098 ; UER00360 .

Family and domain databases

Gene3Di 3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPi MF_00412. ProA.
MF_00456. ProB.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000965. G-glutamylP_reductase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR005766. P5_carboxy_syn.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view ]
PIRSFi PIRSF036429. P5C_syn. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Regulation of proline accumulation in kiwifruit buds with and without hydrogen cyanamide treatment."
    Walton E.F., Podivinsky E., Wu R.-M., Reynolds P.H.S., Young L.W.
    Physiol. Plantarum 102:171-178(1998)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Axillary bud.

Entry informationi

Entry nameiP5CS_ACTDE
AccessioniPrimary (citable) accession number: O04015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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