ID   NLTP6_AMBAR             Reviewed;         118 AA.
AC   O04004;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   24-JAN-2024, entry version 85.
DE   RecName: Full=Non-specific lipid-transfer protein;
DE            Short=LTP;
DE   AltName: Full=Allergen Amb a VI;
DE   AltName: Full=Allergen Ra6;
DE   AltName: Full=Pollen allergen Amb a 6;
DE   AltName: Allergen=Amb a 6;
DE   Flags: Precursor;
OS   Ambrosia artemisiifolia (Common ragweed).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae alliance; Heliantheae; Ambrosia.
OX   NCBI_TaxID=4212;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND VARIANTS PRO-27;
RP   ASP-32; ASN-36; PRO-67; MET-71; VAL-91 AND PHE-111.
RC   TISSUE=Flower;
RX   PubMed=9714407; DOI=10.1046/j.1365-3083.1998.00355.x;
RA   Hiller K.M., Lubahn B.C., Klapper D.G.;
RT   "Cloning and expression of ragweed allergen Amb a 6.";
RL   Scand. J. Immunol. 48:26-36(1998).
RN   [2]
RP   PROTEIN SEQUENCE OF N-TERMINUS, VARIANTS RA6A PRO-27 AND ASN-36, VARIANTS
RP   RA6B ALA-26 DEL AND ALA-32, AND CHARACTERIZATION.
RC   TISSUE=Pollen;
RX   PubMed=6863927;
RA   Roebber M., Hussain R., Klapper D.G., Marsh D.G.;
RT   "Isolation and properties of a new short ragweed pollen allergen, Ra6.";
RL   J. Immunol. 131:706-711(1983).
CC   -!- FUNCTION: Plant non-specific lipid-transfer proteins transfer
CC       phospholipids as well as galactolipids across membranes. May play a
CC       role in wax or cutin deposition in the cell walls of expanding
CC       epidermal cells and certain secretory tissues (By similarity).
CC       {ECO:0000250}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC   -!- SIMILARITY: Belongs to the plant LTP family. {ECO:0000305}.
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DR   EMBL; U89793; AAB51146.1; -; mRNA.
DR   AlphaFoldDB; O04004; -.
DR   SMR; O04004; -.
DR   Allergome; 29; Amb a 6.
DR   Allergome; 3070; Amb a 6.0101.
DR   GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR   GO; GO:0006869; P:lipid transport; IEA:InterPro.
DR   CDD; cd01960; nsLTP1; 1.
DR   Gene3D; 1.10.110.10; Plant lipid-transfer and hydrophobic proteins; 1.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR000528; Plant_nsLTP.
DR   PANTHER; PTHR33076:SF73; AAI DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR33076; NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED; 1.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PRINTS; PR00382; LIPIDTRNSFER.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Disulfide bond; Lipid-binding; Signal;
KW   Transport.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:6863927"
FT   CHAIN           26..118
FT                   /note="Non-specific lipid-transfer protein"
FT                   /id="PRO_0000018360"
FT   DISULFID        30..76
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..53
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..98
FT                   /evidence="ECO:0000250"
FT   DISULFID        74..113
FT                   /evidence="ECO:0000250"
FT   VARIANT         26
FT                   /note="Missing (in Ra6B)"
FT                   /evidence="ECO:0000269|PubMed:6863927"
FT   VARIANT         27
FT                   /note="S -> P (in Ra6A)"
FT                   /evidence="ECO:0000269|PubMed:6863927,
FT                   ECO:0000269|PubMed:9714407"
FT   VARIANT         32
FT                   /note="T -> A (in Ra6B)"
FT                   /evidence="ECO:0000269|PubMed:6863927"
FT   VARIANT         32
FT                   /note="T -> D"
FT                   /evidence="ECO:0000269|PubMed:9714407"
FT   VARIANT         36
FT                   /note="I -> N (in Ra6A)"
FT                   /evidence="ECO:0000269|PubMed:6863927,
FT                   ECO:0000269|PubMed:9714407"
FT   VARIANT         67
FT                   /note="K -> P"
FT                   /evidence="ECO:0000269|PubMed:9714407"
FT   VARIANT         71
FT                   /note="V -> M"
FT                   /evidence="ECO:0000269|PubMed:9714407"
FT   VARIANT         91
FT                   /note="M -> V"
FT                   /evidence="ECO:0000269|PubMed:9714407"
FT   VARIANT         111
FT                   /note="L -> F"
FT                   /evidence="ECO:0000269|PubMed:9714407"
SQ   SEQUENCE   118 AA;  12789 MW;  59356E9196C0CD38 CRC64;
     MDCIRILWSV AVGLLLVSWR PTMFAASPTC DTVQNILAPC AGFLTGQEPS KACCTGVNNL
     NNSRKTKADR VAVCNCIKEL TKSIAYDPKR MPLLSTKCGV KPDFPAVDKN LDCSKLPV
//