O04004 (NLTP6_AMBAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Non-specific lipid-transfer protein Short name=LTP Alternative name(s): Allergen Amb a VI Allergen Ra6 Pollen allergen Amb a 6 Allergen=Amb a 6
Organism	Ambrosia artemisiifolia (Short ragweed)
Taxonomic identifier	4212 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae alliance › Heliantheae › Ambrosia

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues By similarity.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE.
Sequence similarities	Belongs to the plant LTP family.

Ontologies

Keywords
Biological process	Transport
Coding sequence diversity	Polymorphism
Disease	Allergen
Domain	Signal
Ligand	Lipid-binding
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid transport Inferred from electronic annotation. Source: InterPro
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.2
	Chain	26 – 118	93	Non-specific lipid-transfer protein		PRO_0000018360
Amino acid modifications
	Disulfide bond	30 ↔ 76		By similarity
	Disulfide bond	40 ↔ 53		By similarity
	Disulfide bond	54 ↔ 98		By similarity
	Disulfide bond	74 ↔ 113		By similarity
Natural variations
	Natural variant	26	1	Missing in Ra6B. Ref.2
	Natural variant	27	1	S → P in Ra6A. Ref.1 Ref.2
	Natural variant	32	1	T → A in Ra6B. Ref.2
	Natural variant	32	1	T → D. Ref.1
	Natural variant	36	1	I → N in Ra6A. Ref.1 Ref.2
	Natural variant	67	1	K → P. Ref.1
	Natural variant	71	1	V → M. Ref.1
	Natural variant	91	1	M → V. Ref.1
	Natural variant	111	1	L → F. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O04004 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 59356E9196C0CD38	FASTA	118	12,789
	10 20 30 40 50 60 MDCIRILWSV AVGLLLVSWR PTMFAASPTC DTVQNILAPC AGFLTGQEPS KACCTGVNNL 70 80 90 100 110 NNSRKTKADR VAVCNCIKEL TKSIAYDPKR MPLLSTKCGV KPDFPAVDKN LDCSKLPV

References

[1]	"Cloning and expression of ragweed allergen Amb a 6." Hiller K.M., Lubahn B.C., Klapper D.G. Scand. J. Immunol. 48:26-36(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANTS PRO-27; ASP-32; ASN-36; PRO-67; MET-71; VAL-91 AND PHE-111. Tissue: Flower.
[2]	"Isolation and properties of a new short ragweed pollen allergen, Ra6." Roebber M., Hussain R., Klapper D.G., Marsh D.G. J. Immunol. 131:706-711(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, VARIANTS RA6A PRO-27 AND ASN-36, VARIANTS RA6B ALA-26 DEL AND ALA-32, CHARACTERIZATION. Tissue: Pollen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U89793 mRNA. Translation: AAB51146.1.
3D structure databases
ProteinModelPortal	O04004.
ModBase	Search...
Protein family/group databases
Allergome	29. Amb a 6. 3070. Amb a 6.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR000528. Plant_LTP. [Graphical view]
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00382. LIPIDTRNSFER.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NLTP6_AMBAR

Accession

Primary (citable) accession number: O04004

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program