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O03979

- ALYS_BPDP1

UniProt

O03979 - ALYS_BPDP1

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Protein

Lysin

Gene

PAL

Organism
Pneumococcus phage Dp-1 (Bacteriophage Dp-1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Lysis of bacterial host cell wall.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. cytolysis Source: UniProtKB-KW
  3. defense response to bacterium Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Lysin (EC:3.5.1.28)
Alternative name(s):
Cell wall hydrolase
Lytic amidase
N-acetylmuramoyl-L-alanine amidase
Gene namesi
Name:PAL
OrganismiPneumococcus phage Dp-1 (Bacteriophage Dp-1)
Taxonomic identifieri59241 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridae
Virus hostiStreptococcus pneumoniae [TaxID: 1313]

Subcellular locationi

Host cell wall
Note: Binds to the choline residues present in the cell wall substrate.

Pathology & Biotechi

Pharmaceutical usei

May have a use in the prevention of pneumococcal disease. Pal acts very effectively in killing 15 common serotypes of pneumococci, including highly penicillin-resistant strains. It could be used to eliminate the nasopharyngeal reservoir of these bacteria.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296LysinPRO_0000164428Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO03979.
SMRiO03979. Positions 167-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati152 – 17120Cell wall-binding 1Add
BLAST
Repeati173 – 19422Cell wall-binding 2Add
BLAST
Repeati196 – 21621Cell wall-binding 3Add
BLAST
Repeati217 – 23620Cell wall-binding 4Add
BLAST
Repeati237 – 25620Cell wall-binding 5Add
BLAST
Repeati259 – 27820Cell wall-binding 6Add
BLAST

Sequence similaritiesi

Contains 6 cell wall-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.90.1720.10. 1 hit.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR000064. NLP_P60_dom.
IPR008044. Phage_lysin.
[Graphical view]
PfamiPF05382. Amidase_5. 1 hit.
PF01473. CW_binding_1. 5 hits.
[Graphical view]
PROSITEiPS51170. CW. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O03979-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVDIEKGVA WMQARKGRVS YSMDFRDGPD SYDCSSSMYY ALRSAGASSA
60 70 80 90 100
GWAVNTEYMH AWLIENGYEL ISENAPWDAK RGDIFIWGRK GASAGAGGHT
110 120 130 140 150
GMFIDSDNII HCNYAYDGIS VNDHDERWYY AGQPYYYVYR LTNANAQPAE
160 170 180 190 200
KKLGWQKDAT GFWYARANGT YPKDEFEYIE ENKSWFYFDD QGYMLAEKWL
210 220 230 240 250
KHTDGNWYWF DRDGYMATSW KRIGESWYYF NRDGSMVTGW IKYYDNWYYC
260 270 280 290
DATNGDMKSN AFIRYNDGWY LLLPDGRLAD KPQFTVEPDG LITAKV
Length:296
Mass (Da):34,453
Last modified:July 1, 1997 - v1
Checksum:iC17F0911B7D46CC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93946 Genomic DNA. Translation: CAB07986.1.
RefSeqiYP_004306947.1. NC_015274.1.

Genome annotation databases

GeneIDi10358655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93946 Genomic DNA. Translation: CAB07986.1 .
RefSeqi YP_004306947.1. NC_015274.1.

3D structure databases

ProteinModelPortali O03979.
SMRi O03979. Positions 167-295.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 10358655.

Family and domain databases

Gene3Di 3.90.1720.10. 1 hit.
InterProi IPR018337. Cell_wall/Cho-bd_repeat.
IPR000064. NLP_P60_dom.
IPR008044. Phage_lysin.
[Graphical view ]
Pfami PF05382. Amidase_5. 1 hit.
PF01473. CW_binding_1. 5 hits.
[Graphical view ]
PROSITEi PS51170. CW. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The lytic enzyme of the pneumococcal phage Dp-1: a chimeric lysin of intergeneric origin."
    Sheehan M.M., Garcia J.L., Lopez R., Garcia P.
    Mol. Microbiol. 25:717-725(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase."
    Loeffler J.M., Nelson D., Fischetti V.A.
    Science 294:2170-2172(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: USE OF PAL IN ERADICATION OF S.PNEUMONIAE.

Entry informationi

Entry nameiALYS_BPDP1
AccessioniPrimary (citable) accession number: O03979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 1, 1997
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Pharmaceutical

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3