O03979 (ALYS_BPDP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysin
EC=3.5.1.28

Alternative name(s):
Cell wall hydrolase
Lytic amidase
N-acetylmuramoyl-L-alanine amidase

Gene names

Name:

PAL

Organism

Pneumococcus phage Dp-1 (Bacteriophage Dp-1)

Taxonomic identifier

59241 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › unclassified Siphoviridae

Virus host

Streptococcus pneumoniae [TaxID: 1313]

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysis of bacterial host cell wall.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Host cell wall. Note: Binds to the choline residues present in the cell wall substrate.
Pharmaceutical use	May have a use in the prevention of pneumococcal disease. Pal acts very effectively in killing 15 common serotypes of pneumococci, including highly penicillin-resistant strains. It could be used to eliminate the nasopharyngeal reservoir of these bacteria.
Sequence similarities	Contains 6 cell wall-binding repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Domain	Repeat
Molecular function	Antimicrobial Bacteriolytic enzyme Hydrolase
Technical term	Pharmaceutical
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Lysin		PRO_0000164428
Regions
	Repeat	152 – 171	20	Cell wall-binding 1
	Repeat	173 – 194	22	Cell wall-binding 2
	Repeat	196 – 216	21	Cell wall-binding 3
	Repeat	217 – 236	20	Cell wall-binding 4
	Repeat	237 – 256	20	Cell wall-binding 5
	Repeat	259 – 278	20	Cell wall-binding 6

Sequences

	Sequence		Length	Mass (Da)
	O03979 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: C17F0911B7D46CC1	FASTA	296	34,453
	10 20 30 40 50 60 MGVDIEKGVA WMQARKGRVS YSMDFRDGPD SYDCSSSMYY ALRSAGASSA GWAVNTEYMH 70 80 90 100 110 120 AWLIENGYEL ISENAPWDAK RGDIFIWGRK GASAGAGGHT GMFIDSDNII HCNYAYDGIS 130 140 150 160 170 180 VNDHDERWYY AGQPYYYVYR LTNANAQPAE KKLGWQKDAT GFWYARANGT YPKDEFEYIE 190 200 210 220 230 240 ENKSWFYFDD QGYMLAEKWL KHTDGNWYWF DRDGYMATSW KRIGESWYYF NRDGSMVTGW 250 260 270 280 290 IKYYDNWYYC DATNGDMKSN AFIRYNDGWY LLLPDGRLAD KPQFTVEPDG LITAKV

References

[1]	"The lytic enzyme of the pneumococcal phage Dp-1: a chimeric lysin of intergeneric origin." Sheehan M.M., Garcia J.L., Lopez R., Garcia P. Mol. Microbiol. 25:717-725(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase." Loeffler J.M., Nelson D., Fischetti V.A. Science 294:2170-2172(2001) [PubMed] [Europe PMC] [Abstract] Cited for: USE OF PAL IN ERADICATION OF S.PNEUMONIAE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z93946 Genomic DNA. Translation: CAB07986.1.
RefSeq	YP_004306947.1. NC_015274.1.
3D structure databases
ProteinModelPortal	O03979.
SMR	O03979. Positions 167-295.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10358655.
Family and domain databases
Gene3D	3.90.1720.10. 1 hit.
InterPro	IPR018337. Cell_wall/Cho-bd_repeat. IPR000064. NLP_P60_dom. IPR008044. Phage_SFi21_lysin. [Graphical view]
Pfam	PF05382. Amidase_5. 1 hit. PF01473. CW_binding_1. 5 hits. [Graphical view]
PROSITE	PS51170. CW. 6 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALYS_BPDP1

Accession

Primary (citable) accession number: O03979

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	July 1, 1997
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program