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Protein

Lysin

Gene

PAL

Organism
Pneumococcus phage Dp-1 (Bacteriophage Dp-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Lysis of bacterial host cell wall.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Lysin (EC:3.5.1.28)
Alternative name(s):
Cell wall hydrolase
Lytic amidase
N-acetylmuramoyl-L-alanine amidase
Gene namesi
Name:PAL
OrganismiPneumococcus phage Dp-1 (Bacteriophage Dp-1)
Taxonomic identifieri59241 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridae
Virus hostiStreptococcus pneumoniae [TaxID: 1313]

Subcellular locationi

  • Host cell wall

  • Note: Binds to the choline residues present in the cell wall substrate.

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Pharmaceutical usei

May have a use in the prevention of pneumococcal disease. Pal acts very effectively in killing 15 common serotypes of pneumococci, including highly penicillin-resistant strains. It could be used to eliminate the nasopharyngeal reservoir of these bacteria.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296LysinPRO_0000164428Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO03979.
SMRiO03979. Positions 167-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati152 – 17120Cell wall-binding 1Add
BLAST
Repeati173 – 19422Cell wall-binding 2Add
BLAST
Repeati196 – 21621Cell wall-binding 3Add
BLAST
Repeati217 – 23620Cell wall-binding 4Add
BLAST
Repeati237 – 25620Cell wall-binding 5Add
BLAST
Repeati259 – 27820Cell wall-binding 6Add
BLAST

Sequence similaritiesi

Contains 6 cell wall-binding repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.90.1720.10. 1 hit.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR000064. NLP_P60_dom.
IPR008044. Phage_lysin.
[Graphical view]
PfamiPF05382. Amidase_5. 1 hit.
PF01473. CW_binding_1. 5 hits.
[Graphical view]
PROSITEiPS51170. CW. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O03979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVDIEKGVA WMQARKGRVS YSMDFRDGPD SYDCSSSMYY ALRSAGASSA
60 70 80 90 100
GWAVNTEYMH AWLIENGYEL ISENAPWDAK RGDIFIWGRK GASAGAGGHT
110 120 130 140 150
GMFIDSDNII HCNYAYDGIS VNDHDERWYY AGQPYYYVYR LTNANAQPAE
160 170 180 190 200
KKLGWQKDAT GFWYARANGT YPKDEFEYIE ENKSWFYFDD QGYMLAEKWL
210 220 230 240 250
KHTDGNWYWF DRDGYMATSW KRIGESWYYF NRDGSMVTGW IKYYDNWYYC
260 270 280 290
DATNGDMKSN AFIRYNDGWY LLLPDGRLAD KPQFTVEPDG LITAKV
Length:296
Mass (Da):34,453
Last modified:July 1, 1997 - v1
Checksum:iC17F0911B7D46CC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93946 Genomic DNA. Translation: CAB07986.1.
RefSeqiYP_004306947.1. NC_015274.1.

Genome annotation databases

GeneIDi10358655.
KEGGivg:10358655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z93946 Genomic DNA. Translation: CAB07986.1.
RefSeqiYP_004306947.1. NC_015274.1.

3D structure databases

ProteinModelPortaliO03979.
SMRiO03979. Positions 167-295.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi10358655.
KEGGivg:10358655.

Family and domain databases

Gene3Di3.90.1720.10. 1 hit.
InterProiIPR018337. Cell_wall/Cho-bd_repeat.
IPR000064. NLP_P60_dom.
IPR008044. Phage_lysin.
[Graphical view]
PfamiPF05382. Amidase_5. 1 hit.
PF01473. CW_binding_1. 5 hits.
[Graphical view]
PROSITEiPS51170. CW. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The lytic enzyme of the pneumococcal phage Dp-1: a chimeric lysin of intergeneric origin."
    Sheehan M.M., Garcia J.L., Lopez R., Garcia P.
    Mol. Microbiol. 25:717-725(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase."
    Loeffler J.M., Nelson D., Fischetti V.A.
    Science 294:2170-2172(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: USE OF PAL IN ERADICATION OF S.PNEUMONIAE.

Entry informationi

Entry nameiALYS_BPDP1
AccessioniPrimary (citable) accession number: O03979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 1, 1997
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Pharmaceutical

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.