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O03979 (ALYS_BPDP1) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysin

EC=3.5.1.28
Alternative name(s):
Cell wall hydrolase
Lytic amidase
N-acetylmuramoyl-L-alanine amidase
Gene names
Name:PAL
OrganismPneumococcus phage Dp-1 (Bacteriophage Dp-1)
Taxonomic identifier59241 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridae
Virus hostStreptococcus pneumoniae [TaxID: 1313]

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Lysis of bacterial host cell wall.

Catalytic activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Subcellular location

Host cell wall. Note: Binds to the choline residues present in the cell wall substrate.

Pharmaceutical use

May have a use in the prevention of pneumococcal disease. Pal acts very effectively in killing 15 common serotypes of pneumococci, including highly penicillin-resistant strains. It could be used to eliminate the nasopharyngeal reservoir of these bacteria.

Sequence similarities

Contains 6 cell wall-binding repeats.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   DomainRepeat
   Molecular functionAntimicrobial
Bacteriolytic enzyme
Hydrolase
   Technical termPharmaceutical
Gene Ontology (GO)
   Biological_processcytolysis

Inferred from electronic annotation. Source: UniProtKB-KW

defense response to bacterium

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionN-acetylmuramoyl-L-alanine amidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Lysin
PRO_0000164428

Regions

Repeat152 – 17120Cell wall-binding 1
Repeat173 – 19422Cell wall-binding 2
Repeat196 – 21621Cell wall-binding 3
Repeat217 – 23620Cell wall-binding 4
Repeat237 – 25620Cell wall-binding 5
Repeat259 – 27820Cell wall-binding 6

Sequences

Sequence LengthMass (Da)Tools
O03979 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: C17F0911B7D46CC1

FASTA29634,453
        10         20         30         40         50         60 
MGVDIEKGVA WMQARKGRVS YSMDFRDGPD SYDCSSSMYY ALRSAGASSA GWAVNTEYMH 

        70         80         90        100        110        120 
AWLIENGYEL ISENAPWDAK RGDIFIWGRK GASAGAGGHT GMFIDSDNII HCNYAYDGIS 

       130        140        150        160        170        180 
VNDHDERWYY AGQPYYYVYR LTNANAQPAE KKLGWQKDAT GFWYARANGT YPKDEFEYIE 

       190        200        210        220        230        240 
ENKSWFYFDD QGYMLAEKWL KHTDGNWYWF DRDGYMATSW KRIGESWYYF NRDGSMVTGW 

       250        260        270        280        290 
IKYYDNWYYC DATNGDMKSN AFIRYNDGWY LLLPDGRLAD KPQFTVEPDG LITAKV 

« Hide

References

[1]"The lytic enzyme of the pneumococcal phage Dp-1: a chimeric lysin of intergeneric origin."
Sheehan M.M., Garcia J.L., Lopez R., Garcia P.
Mol. Microbiol. 25:717-725(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase."
Loeffler J.M., Nelson D., Fischetti V.A.
Science 294:2170-2172(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: USE OF PAL IN ERADICATION OF S.PNEUMONIAE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z93946 Genomic DNA. Translation: CAB07986.1.
RefSeqYP_004306947.1. NC_015274.1.

3D structure databases

ProteinModelPortalO03979.
SMRO03979. Positions 167-295.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID10358655.

Family and domain databases

Gene3D3.90.1720.10. 1 hit.
InterProIPR018337. Cell_wall/Cho-bd_repeat.
IPR000064. NLP_P60_dom.
IPR008044. Phage_lysin.
[Graphical view]
PfamPF05382. Amidase_5. 1 hit.
PF01473. CW_binding_1. 5 hits.
[Graphical view]
PROSITEPS51170. CW. 6 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALYS_BPDP1
AccessionPrimary (citable) accession number: O03979
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 1, 1997
Last modified: October 16, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families