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Protein

Ribulose bisphosphate carboxylase large chain

Gene

rbcL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123Substrate; in homodimeric partnerUniRule annotation1
Binding sitei173SubstrateUniRule annotation1
Active sitei175Proton acceptorUniRule annotation1
Binding sitei177SubstrateUniRule annotation1
Metal bindingi201Magnesium; via carbamate groupUniRule annotation1
Metal bindingi203MagnesiumUniRule annotation1
Metal bindingi204MagnesiumUniRule annotation1
Active sitei294Proton acceptorUniRule annotation1
Binding sitei295SubstrateUniRule annotation1
Binding sitei327SubstrateUniRule annotation1
Sitei334Transition state stabilizerUniRule annotation1
Binding sitei379SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • photorespiration Source: UniProtKB-KW
  • reductive pentose-phosphate cycle Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionLyase, Monooxygenase, Oxidoreductase
Biological processCalvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:ATCG00490-MONOMER.
MetaCyc:ATCG00490-MONOMER.
BRENDAi4.1.1.39. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:AtCg00490
Encoded oniPlastid; Chloroplast
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chloroplast

Organism-specific databases

AraportiATCG00490.
TAIRilocus:504954672. ATCG00490.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000311191 – 2UniRule annotationCombined sources2
ChainiPRO_00000311203 – 479Ribulose bisphosphate carboxylase large chainAdd BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N-acetylprolineCombined sources1
Modified residuei201N6-carboxylysineUniRule annotation1
Modified residuei208PhosphoserineCombined sources1
Disulfide bondi247Interchain; in linked formUniRule annotation
Modified residuei330PhosphothreonineCombined sources1

Post-translational modificationi

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover.UniRule annotation

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiO03042.
PRIDEiO03042.

2D gel databases

SWISS-2DPAGEiO03042.

PTM databases

iPTMnetiO03042.

Expressioni

Gene expression databases

ExpressionAtlasiO03042. baseline and differential.
GenevisibleiO03042. AT.

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers (By similarity). Interacts with RBCX1 and RBCX1 (PubMed:21922322).UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi29958. 5 interactors.
IntActiO03042. 2 interactors.
STRINGi3702.ATCG00490.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IU0X-ray1.50A/B1-479[»]
ProteinModelPortaliO03042.
SMRiO03042.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410IIVP. Eukaryota.
COG1850. LUCA.
HOGENOMiHOG000230831.
InParanoidiO03042.
KOiK01601.
OMAiHRAMHAA.
OrthoDBiEOG093607MT.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiView protein in InterPro
IPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR036376. RuBisCO_lsu_C_sf.
IPR017443. RuBisCO_lsu_fd_N.
IPR036422. RuBisCO_lsu_N_sf.
IPR020888. RuBisCO_lsuI.
PfamiView protein in Pfam
PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
SFLDiSFLDS00014. RuBisCO. 1 hit.
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiView protein in PROSITE
PS00157. RUBISCO_LARGE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O03042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPQTETKAS VGFKAGVKEY KLTYYTPEYE TKDTDILAAF RVTPQPGVPP
60 70 80 90 100
EEAGAAVAAE SSTGTWTTVW TDGLTSLDRY KGRCYHIEPV PGEETQFIAY
110 120 130 140 150
VAYPLDLFEE GSVTNMFTSI VGNVFGFKAL AALRLEDLRI PPAYTKTFQG
160 170 180 190 200
PPHGIQVERD KLNKYGRPLL GCTIKPKLGL SAKNYGRAVY ECLRGGLDFT
210 220 230 240 250
KDDENVNSQP FMRWRDRFLF CAEAIYKSQA ETGEIKGHYL NATAGTCEEM
260 270 280 290 300
IKRAVFAREL GVPIVMHDYL TGGFTANTSL SHYCRDNGLL LHIHRAMHAV
310 320 330 340 350
IDRQKNHGMH FRVLAKALRL SGGDHIHAGT VVGKLEGDRE STLGFVDLLR
360 370 380 390 400
DDYVEKDRSR GIFFTQDWVS LPGVLPVASG GIHVWHMPAL TEIFGDDSVL
410 420 430 440 450
QFGGGTLGHP WGNAPGAVAN RVALEACVQA RNEGRDLAVE GNEIIREACK
460 470
WSPELAAACE VWKEITFNFP TIDKLDGQE
Length:479
Mass (Da):52,955
Last modified:July 1, 1997 - v1
Checksum:i3086705F3C8FEF75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91966 Genomic DNA. Translation: AAB68400.1.
AP000423 Genomic DNA. Translation: BAA84393.1.
AB003522 Genomic DNA. Translation: BAA20946.1.
D88901 Genomic DNA. Translation: BAA19595.1.
RefSeqiNP_051067.1. NC_000932.1.

Genome annotation databases

EnsemblPlantsiATCG00490.1; ATCG00490.1; ATCG00490.
GeneIDi844754.
GrameneiATCG00490.1; ATCG00490.1; ATCG00490.
KEGGiath:ArthCp030.

Similar proteinsi

Entry informationi

Entry nameiRBL_ARATH
AccessioniPrimary (citable) accession number: O03042
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: October 25, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families