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O02853

- PTGDS_BOVIN

UniProt

O02853 - PTGDS_BOVIN

Protein

Prostaglandin-H2 D-isomerase

Gene

PTGDS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system By similarity.By similarity

    Catalytic activityi

    (5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651NucleophileBy similarity

    GO - Molecular functioni

    1. fatty acid binding Source: Ensembl
    2. prostaglandin-D synthase activity Source: UniProtKB
    3. retinoid binding Source: UniProtKB
    4. transporter activity Source: UniProtKB

    GO - Biological processi

    1. prostaglandin biosynthetic process Source: UniProtKB
    2. regulation of circadian sleep/wake cycle, sleep Source: UniProtKB
    3. transport Source: UniProtKB

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism, Transport

    Enzyme and pathway databases

    ReactomeiREACT_214914. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prostaglandin-H2 D-isomerase (EC:5.3.99.2)
    Alternative name(s):
    Glutathione-independent PGD synthase
    Lipocalin-type prostaglandin-D synthase
    Prostaglandin-D2 synthase
    Short name:
    PGD2 synthase
    Short name:
    PGDS
    Short name:
    PGDS2
    Gene namesi
    Name:PTGDS
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 11

    Subcellular locationi

    Rough endoplasmic reticulum By similarity. Nucleus membrane By similarity. Golgi apparatus By similarity. Cytoplasmperinuclear region By similarity. Secreted By similarity
    Note: Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted By similarity.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: UniProtKB
    3. Golgi apparatus Source: UniProtKB
    4. nuclear membrane Source: UniProtKB-SubCell
    5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    6. rough endoplasmic reticulum Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 191163Prostaglandin-H2 D-isomerasePRO_0000017940Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi51 – 511N-linked (GlcNAc...)By similarity
    Glycosylationi78 – 781N-linked (GlcNAc...)By similarity
    Disulfide bondi89 ↔ 186By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO02853.
    PRIDEiO02853.

    Expressioni

    Tissue specificityi

    In the male reproductive system, expressed in the testis, epididymis and prostate, and secreted into the seminal fluid.3 Publications

    Developmental stagei

    During spermatogenesis, expression is low in the round spermatids of stages I-II before increasing to peak in the elongating spermatids of stages III-VII. Decreased expression observed in stage VIII.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO02853.
    SMRiO02853. Positions 30-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.By similarity

    Sequence similaritiesi

    Belongs to the calycin superfamily. Lipocalin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG45731.
    GeneTreeiENSGT00620000088005.
    HOGENOMiHOG000231660.
    HOVERGENiHBG106490.
    InParanoidiO02853.
    KOiK01830.
    OMAiPGQDFRM.
    OrthoDBiEOG78PVBH.
    TreeFamiTF336103.

    Family and domain databases

    Gene3Di2.40.128.20. 1 hit.
    InterProiIPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR002345. Lipocalin.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002972. PstgldnD_synth.
    [Graphical view]
    PfamiPF00061. Lipocalin. 1 hit.
    [Graphical view]
    PRINTSiPR00179. LIPOCALIN.
    PR01254. PGNDSYNTHASE.
    SUPFAMiSSF50814. SSF50814. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O02853-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPNRLWMA LLLLGVLGVL QTPAPAQAAL QPNFEEDKFL GRWFTSGLAS    50
    NSSWFLEKKK VLSMCKSVVA PAADGGLNLT STFLRKDQCE TRTLLLRPAG 100
    PPGCYSYTSP HWSSTHEVSV AETDYETYAL LYTEGVRGPG QDFRMATLYS 150
    RSQNPRAEVK EHFTTFAKSL GFTEEGIVFL PKTDKCMEEH P 191
    Length:191
    Mass (Da):21,229
    Last modified:July 1, 1997 - v1
    Checksum:i3B55675987B7A4C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004647 mRNA. Translation: BAA20431.1.
    BC133435 mRNA. Translation: AAI33436.1.
    BC133438 mRNA. Translation: AAI33439.1.
    BC133445 mRNA. Translation: AAI33446.1.
    BC142082 mRNA. Translation: AAI42083.1.
    RefSeqiNP_777216.1. NM_174791.4.
    UniGeneiBt.1645.

    Genome annotation databases

    EnsembliENSBTAT00000020065; ENSBTAP00000020065; ENSBTAG00000015074.
    GeneIDi286858.
    KEGGibta:286858.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004647 mRNA. Translation: BAA20431.1 .
    BC133435 mRNA. Translation: AAI33436.1 .
    BC133438 mRNA. Translation: AAI33439.1 .
    BC133445 mRNA. Translation: AAI33446.1 .
    BC142082 mRNA. Translation: AAI42083.1 .
    RefSeqi NP_777216.1. NM_174791.4.
    UniGenei Bt.1645.

    3D structure databases

    ProteinModelPortali O02853.
    SMRi O02853. Positions 30-189.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi O02853.
    ChEMBLi CHEMBL4651.

    Proteomic databases

    PaxDbi O02853.
    PRIDEi O02853.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000020065 ; ENSBTAP00000020065 ; ENSBTAG00000015074 .
    GeneIDi 286858.
    KEGGi bta:286858.

    Organism-specific databases

    CTDi 5730.

    Phylogenomic databases

    eggNOGi NOG45731.
    GeneTreei ENSGT00620000088005.
    HOGENOMi HOG000231660.
    HOVERGENi HBG106490.
    InParanoidi O02853.
    KOi K01830.
    OMAi PGQDFRM.
    OrthoDBi EOG78PVBH.
    TreeFami TF336103.

    Enzyme and pathway databases

    Reactomei REACT_214914. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

    Miscellaneous databases

    NextBioi 20806507.

    Family and domain databases

    Gene3Di 2.40.128.20. 1 hit.
    InterProi IPR012674. Calycin.
    IPR011038. Calycin-like.
    IPR002345. Lipocalin.
    IPR000566. Lipocln_cytosolic_FA-bd_dom.
    IPR002972. PstgldnD_synth.
    [Graphical view ]
    Pfami PF00061. Lipocalin. 1 hit.
    [Graphical view ]
    PRINTSi PR00179. LIPOCALIN.
    PR01254. PGNDSYNTHASE.
    SUPFAMi SSF50814. SSF50814. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a fertility-associated protein in bull seminal plasma as lipocalin-type prostaglandin D synthase."
      Gerena R.L., Irikura D., Urade Y., Eguchi N., Chapman D.A., Killian G.J.
      Biol. Reprod. 58:826-833(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-48, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal pons and Hypothalamus.
    3. "Immunocytochemical localization of lipocalin-type prostaglandin D synthase in the bull testis and epididymis and on ejaculated sperm."
      Gerena R.L., Irikura D., Eguchi N., Urade Y., Killian G.J.
      Biol. Reprod. 62:547-556(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Mammalian lipocalin-type prostaglandin D2 synthase in the fluids of the male genital tract: putative biochemical and physiological functions."
      Fouchecourt S., Charpigny G., Reinaud P., Dumont P., Dacheux J.-L.
      Biol. Reprod. 66:458-467(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPTGDS_BOVIN
    AccessioniPrimary (citable) accession number: O02853
    Secondary accession number(s): A2VDW5, A5PJE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3