Reviewed, UniProtKB/Swiss-Prot O02811 (PI4KA_BOVIN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase alpha
      Short name=PI4-kinase alpha
      Short name=PI4K-alpha
    EC=2.7.1.67

Gene names

Name:	PI4KA
Synonyms:	PIK4CA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	2043 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Enzyme regulation	Inhibited by wortmannin. Ref.1
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2043	2043	Phosphatidylinositol 4-kinase alpha		PRO_0000226047
Regions
	Domain	1787 – 2020	234	PI3K/PI4K
Amino acid modifications
	Modified residue	172	1	Phosphoserine By similarity
	Modified residue	198	1	Phosphoserine By similarity
	Modified residue	199	1	Phosphoserine By similarity
	Modified residue	202	1	Phosphoserine By similarity
	Modified residue	207	1	Phosphoserine By similarity
	Modified residue	1095	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02811-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 51E20B92183C318D	FASTA	2,043	229,392
	10 20 30 40 50 60 MCPVDFHGIF QLDERRRDAV IALGIFLIES DLQHKDSVVP YLLRLLKGLP KVYWVEESTA 70 80 90 100 110 120 RKGRGTLPVA ESLSFCLVTL LSDVAYRDPS LRGEILEALL QVLHVLLGMC QALEIQEKEY 130 140 150 160 170 180 LCKHAVPCLL GIVRAFGRHS SSEDSLLSRL FPRAPAHSPR VPEELEGVRR RSFNDFRSIL 190 200 210 220 230 240 PSSLLTVCQE GSLKRKASSA SSVAQVSPER GAPPPSPPGG SAFHCLEASY SPNGSTSESD 250 260 270 280 290 300 YYFSAVSSSL SVSPLFNGVT YKEFSIPLEM LRELLNLVKK IVEEPVLKSL DAVVTGVIEA 310 320 330 340 350 360 SPSADLCHGA FSDPLYVAVL RMLRDTLYYM RDLPTSFVKE VHDFVLEQFN SSQGELQKIL 370 380 390 400 410 420 HDADRAHSEL SPLKLRCQAS AACVDLMVWA VKDEQGAENL CVKLSEKLQS KTSSKVIIAQ 430 440 450 460 470 480 VPLLICCLQG LGRLCERFPV VVHSVTPSLR DFLVVPSPVL VKLYKCHSQY HTVAGNDIKI 490 500 510 520 530 540 SVTNEHAEST LNVASGKKSQ PSMYEQLRDI AIDNVCRCLK AGLTEDPVIV EAFLASLSNR 550 560 570 580 590 600 LYISQESDKD AHLIPDHTIR ALGHIAVALR DTPKVMEPIL QILQQKFCQP PSPLDVLIID 610 620 630 640 650 660 QLGCLVITGN QYIYQEVWNL FQQISVKASS VVYSATKDYK DHGYRHCSLA VINALANIAA 670 680 690 700 710 720 NIQDEHLVDE LLMNLLELFV QLGLEGKRAS ERASEKGPAL KASSSAGNLG VLIPVIAVLT 730 740 750 760 770 780 RRLPPIEEPK PRLQKLFRDF WLYSVLMGFA VEGSGLWPEE WYEGVCEIAT KSPLLTFPSK 790 800 810 820 830 840 EPLRSVLQYN SAMKNDTVTP AELSELRSTI VNLLDPAPEV SALIGKLDFA MSTYLLSVYR 850 860 870 880 890 900 LEYMRVLRSS DPARFQVMLC YFEDKAIQKD KSGMMQCVIA VADKVFDAFL NMMAEKAKTK 910 920 930 940 950 960 ENEEELERTQ FLLVSFNHVH KRIRRVADKY LSGLVDKFPH LLWSGTVLKT MLDILQTLSL 970 980 990 1000 1010 1020 SLSADIHKDQ PYYDIPDVPY RITVPDTYEA RESIVKDFAA RCGMILQEAM KWAPTVTKSH 1030 1040 1050 1060 1070 1080 LQEYLSKHQN WVSGLSQHTG LAMATESVLH YSGYNKQSTS LGATQLTERP ACVKKDYSNF 1090 1100 1110 1120 1130 1140 MASLNLRNRY AAEVYGMIRF SDATGHTSDL NKMMVQELKA ALGAGDAQQY TQAMFKLTAM 1150 1160 1170 1180 1190 1200 LISSRDCDPQ LLHHLCWGPL QMFNEHGMET ALACWEWLLA GKNGVEVPFM REMAGAWQMT 1210 1220 1230 1240 1250 1260 VEQKFGLFSA EMKEADPLAA SEASQPKPCA PEVTPHYIWI DFLVQRFEIA KYCSSDQVEI 1270 1280 1290 1300 1310 1320 FCSLLQRSLS LSIGGTAGSM NRHVAAIGPR FKLLTLGLSL LHADVLPNAT IRNVLREKIY 1330 1340 1350 1360 1370 1380 STAFDYFSCP PRFPTQGEKR LREDISVMIK FWTAMFSDKK YLTASQLVPP DNQDTRSNLD 1390 1400 1410 1420 1430 1440 IAVGSRQQAT QGWINTYPLS SGMSTISKKS GMSKKTNRGS QLHKYYMKRR TLLLSLLATE 1450 1460 1470 1480 1490 1500 IERLITWYNP LSAPELELDQ AGESSVANWR SKYISLSEKQ WKDNVNLAWS ISPHLAVQLP 1510 1520 1530 1540 1550 1560 ARFKNTEAIG NEVTRLVRLD PGAVSDVPEA IKFLVTWHTI DADAPELSHV LCWAPADPPT 1570 1580 1590 1600 1610 1620 GLSYFSSMSP PHPLTAQYGV KVLRSFPPDA ILFYIPQIVQ ALRYDKMGYV REYILWAASQ 1630 1640 1650 1660 1670 1680 SQLLAHQFIW NMKTNIYVDE EGHQKDPDIG DLLEQLVEEI TGSLSGPAKD FYQREFDFFN 1690 1700 1710 1720 1730 1740 KITNVSAIIK PYPKGDERKK ACLSALSEVK VQPGCYLPSN PEAIVLDIDY KSGTPMQSAA 1750 1760 1770 1780 1790 1800 KAPYLAKFKV KRCGVSELEK EGLRCRSDPE EEGSMQEADG QKISWQAAIF KVGDDCRQDM 1810 1820 1830 1840 1850 1860 LALQIIDLFK NIFQLVGLDL FVFPYRVVAT APGCGVIECI PDCTSRDQLG RQTDFGMYDY 1870 1880 1890 1900 1910 1920 FTRQYGDEST LAFQQARYNF IRSMAAYSLL LFLLQIKDRH NGNIMLDKKG HLIHIDFGFM 1930 1940 1950 1960 1970 1980 FESSPGGNLG WEPDIKLTDE MVMIMGGKME ATPFKWFMEM CVRGYLAVRP YMDAVVSLVT 1990 2000 2010 2020 2030 2040 LMLDTGLPCF RGQTIKLLKH RFSPNMTERE AANFILKVIQ NCFLSNRSRT YDMIQYYQND IPY

References

[1]

"Isolation and molecular cloning of wortmannin-sensitive bovine type III phosphatidylinositol 4-kinases." Balla T., Downing G.J., Jaffe H., Kim S., Zolyomi A., Catt K.J. J. Biol. Chem. 272:18358-18366(1997) [PubMed: 9218477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U88532 mRNA. Translation: AAC48730.1.
IPI	IPI00701244.
RefSeq	NP_777002.1.
UniGene	Bt.14142
3D structure databases
HSSP	HSSP built from PDB template 1E8Y based on UniProtKB P48736.
ModBase	Search...
Protein-protein interaction databases
STRING	O02811.
Genome annotation databases
Ensembl	ENSBTAT00000018572; ENSBTAP00000018572; ENSBTAG00000013971; Bos taurus. [Genome view]
GeneID	282309.
KEGG	bta:282309.
Organism-specific databases
CTD	282309.
Phylogenomic databases
HOVERGEN	O02811.
Enzyme and pathway databases
BRENDA	2.7.1.67. 251.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR001263. PI3Ka. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit. G3DSA:1.25.40.70. PI3Ka. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI4KA_BOVIN

Accession

Primary (citable) accession number: O02811

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 1, 1997
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)