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Protein

Phosphatidylinositol 4-kinase alpha

Gene

PI4KA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.By similarity

Enzyme regulationi

Activated by Triton X-100, insensitive to inhibition by adenosine and inhibited by wortmannin. The PI4K complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns4P) synthesis (By similarity). Interaction with TMEM150A regulates PtdIns4P synthesis (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-1483248. Synthesis of PIPs at the ER membrane.
R-BTA-1660514. Synthesis of PIPs at the Golgi membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase alpha (EC:2.7.1.67By similarity)
Short name:
PI4-kinase alpha
Short name:
PI4K-alpha
Short name:
PtdIns-4-kinase alpha
Gene namesi
Name:PI4KA
Synonyms:PIK4CA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componentsi: Unassembled WGS sequence, Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Localization to the plasma membrane is mediated by the PI4K complex and association with EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B). Localization to the plasma membrane is regulated by TMEM150A.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3185.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002260471 – 2102Phosphatidylinositol 4-kinase alphaAdd BLAST2102

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230PhosphoserineBy similarity1
Modified residuei256PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1
Modified residuei259PhosphoserineBy similarity1
Modified residuei260PhosphoserineBy similarity1
Modified residuei265PhosphoserineBy similarity1
Modified residuei429PhosphoserineBy similarity1
Modified residuei1154PhosphotyrosineBy similarity1
Modified residuei1436PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO02811.
PRIDEiO02811.

Expressioni

Gene expression databases

BgeeiENSBTAG00000013971.

Interactioni

Subunit structurei

Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B). Interacts with TMEM150A; regulating recruitment to the plasma membrane.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018572.

Structurei

3D structure databases

ProteinModelPortaliO02811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1530 – 1718PIK helicalPROSITE-ProRule annotationAdd BLAST189
Domaini1846 – 2079PI3K/PI4KPROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0902. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000007272.
HOVERGENiHBG052742.
InParanoidiO02811.
KOiK00888.
OrthoDBiEOG091G03W5.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 5 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O02811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAARGGG GGGGGGSFGS GAGGGASRGF YFNTVLSLAR SLAVQRPASL
60 70 80 90 100
EKVQKLLCMC PVDFHGIFQL DERRRDAVIA LGIFLIESDL QHKDSVVPYL
110 120 130 140 150
LRLLKGLPKV YWVEESTARK GRGTLPVAES FSFCLVTLLS DVAYRDPSLR
160 170 180 190 200
GEILEALLQV LHVLLGMCQA LEIQEKEYLC KHAVPCLLGI VRAFGRHSSS
210 220 230 240 250
EDSILSRLFP RAPAHSPRVP EELEGVRRRS FNDFRSILPS SLLTVCQEGS
260 270 280 290 300
LKRKASSASS VAQVSPERGA PPPSPPGGSA FHCLEASYSP NGSTSESDYY
310 320 330 340 350
FSAVSSSFSV SPLFNGVTYK EFSIPLEMLR ELLNLVKKIV EEPVLKSLDA
360 370 380 390 400
VVTGVIEASP SADLCYGAFS DPLYVAVLRM LRDTLYYMRD LPTSFVKEVH
410 420 430 440 450
DFVLEQFNSS QGELQKILHD ADRAHSELSP LKLRCQASAA CVDLMVWAVK
460 470 480 490 500
DEQGAENLCV KLSEKLQSKT SSKVIIAHLP LLICCLQGLG RLCERFPVVV
510 520 530 540 550
HSVTPSLRDF LVVPSPVLVK LYKCHSQYHT VAGNDIKISV TNEHAESTLN
560 570 580 590 600
VASGKKSQPS MYEQLRDIAI DNVCRCLKAG LTVDPVIVEA FLASLSNRLY
610 620 630 640 650
ISQESDKDAH LIPDHTIRAL GHIAVALRDT PKVMEPILQI LQQKFCQPPS
660 670 680 690 700
PLDVLIIDQL GCLVITGNQY IYQEVWNLFQ QISVKASSVV YSATKDYKDH
710 720 730 740 750
GYRHCSLAVI NALANIAANI QDEHLVDELL MNLLELFVQL GLEGKRASER
760 770 780 790 800
ASEKGPALKA SSSAGNLGVL IPVIAVLTRR LPPIKEPKPR LQKLFRDFWL
810 820 830 840 850
YSVLMGFAVE GSGLWPEEWY EGVCEIATKS PLLTFPSKEP LRSVLQYNSA
860 870 880 890 900
MKNDTVTPAE LSELRSTIVN LLDPAPEVSA LIGKLDFAMS TYLLSVYRLE
910 920 930 940 950
YMRVLRSSDP ARFQVMFCYF EDKAIQKDKS GMMQCVIAVA DKVFDAFLNM
960 970 980 990 1000
MAEKAKTKEN EEELERHAQF LLVSFNHVHK RIRRVADKYL SGLVDKFPHL
1010 1020 1030 1040 1050
LWSGTVLKTM LDILQTLSLS LSADIHKDQP YYDIPDVPYR ITVPDTYEAR
1060 1070 1080 1090 1100
ESIVKDFAAR CGMILQEAMK WAPTVTKSHL QEYLSKHQNW VSGLSQHTGL
1110 1120 1130 1140 1150
AMATESVLHY AGYNKQSTSL GATQLTERPA CVKKDYSNFM ASLNLRNRYA
1160 1170 1180 1190 1200
GEVYGMIRFS DATGHTSDLN KMMVQELKAA LGAGDAQQYT QAMFKLTAML
1210 1220 1230 1240 1250
ISSRDCDPQL LHHLCWGPLQ MFNEHGMETA LACWEWLLAG KNGVEVPFMR
1260 1270 1280 1290 1300
EMAGAWQMTV EQKFGLFSAE MKEADPLAAS EASQPKPCAP EVTPHYIWID
1310 1320 1330 1340 1350
FLVQRFEIAK YCSSDQVEIF CSLLQRSLSL SIGGTAGSMN RHVAAIGPRF
1360 1370 1380 1390 1400
KLLTLGLSLL HADVLPNATI RNVLREKIYS TAFDYFSCPP RFPTQGEKRL
1410 1420 1430 1440 1450
REDISVMIKF WTAMFSDKKY LTASQLVPPD NQDTRSNLDI AVGSRQQATQ
1460 1470 1480 1490 1500
GWINTYPLSS GMSTISKKSG MSKKTNRGSQ LHKYYMKRRT LLLSLLATEI
1510 1520 1530 1540 1550
ERLITWYNPL SAPELELDQA GESSVANWRS KYISLSEKQW KDNVNLAWSI
1560 1570 1580 1590 1600
SPHLAVQLPA RFKNTEAIGN EVTRLVRLDP GAVSDVPEAI KFLVTWHTID
1610 1620 1630 1640 1650
ADAPELSHVL CWAPADPPTG LSYFSSMYPP HPLTAQYGVK VLRSFPPDAI
1660 1670 1680 1690 1700
LFYIPQIVQA LRYDKMGYVR EYILWAASQS QLLAHQFIWN MKTNIYVDEE
1710 1720 1730 1740 1750
GHQKDPDIGD LLEQLVEEIT GSLSGPAKDF YQREFDFFNK ITNVSAIIKP
1760 1770 1780 1790 1800
YPKGDERKKA CLSALSEVKV QPGCYLPSNP EAIVLDIDYK SGTPMQSAAK
1810 1820 1830 1840 1850
APYLAKFKVK RCGVSELEKE GLRCRSDPEE EGSMQEADGQ KISWQAAIFK
1860 1870 1880 1890 1900
VGDDCRQDML ALQIIDLFKN IFQLVGLDLF VFPYRVVATA PGCGVIECIP
1910 1920 1930 1940 1950
DCTSRDQLGR QTDFGMYDYF TRQYGDESTL AFQQARYNFI RSMAAYSLLL
1960 1970 1980 1990 2000
FLLQIKDRHN GNIMLDKKGH LIHIDFGFMF ESSPGGNLGW EPDIKLTDEM
2010 2020 2030 2040 2050
VMIMGGKMEA TPFKWFMEMC VRGYLAVRPY MDAVVSLVTL MLDTGLPCFR
2060 2070 2080 2090 2100
GQTIKLLKHR FSPNMTEREA ANFILKVIQN CFLSNRSRTY DMIQYYQNDI

PY
Length:2,102
Mass (Da):235,149
Last modified:February 17, 2016 - v2
Checksum:iF9A5F0E9B7FD2F2E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49 – 50SL → CV in AAC48730 (PubMed:9218477).Curated2
Sequence conflicti131F → L in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti204I → L in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti308F → L in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti366Y → H in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti478 – 479HL → QV in AAC48730 (PubMed:9218477).Curated2
Sequence conflicti583V → E in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti785K → E in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti917F → L in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti967 – 968HA → T in AAC48730 (PubMed:9218477).Curated2
Sequence conflicti1111A → S in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti1151G → A in AAC48730 (PubMed:9218477).Curated1
Sequence conflicti1628Y → S in AAC48730 (PubMed:9218477).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88532 mRNA. Translation: AAC48730.1.
DAAA02045733 Genomic DNA. No translation available.
RefSeqiNP_777002.1. NM_174577.2.
UniGeneiBt.14142.

Genome annotation databases

GeneIDi282309.
KEGGibta:282309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88532 mRNA. Translation: AAC48730.1.
DAAA02045733 Genomic DNA. No translation available.
RefSeqiNP_777002.1. NM_174577.2.
UniGeneiBt.14142.

3D structure databases

ProteinModelPortaliO02811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018572.

Chemistry databases

ChEMBLiCHEMBL3185.

Proteomic databases

PaxDbiO02811.
PRIDEiO02811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282309.
KEGGibta:282309.

Organism-specific databases

CTDi5297.

Phylogenomic databases

eggNOGiKOG0902. Eukaryota.
COG5032. LUCA.
HOGENOMiHOG000007272.
HOVERGENiHBG052742.
InParanoidiO02811.
KOiK00888.
OrthoDBiEOG091G03W5.

Enzyme and pathway databases

ReactomeiR-BTA-1483248. Synthesis of PIPs at the ER membrane.
R-BTA-1660514. Synthesis of PIPs at the Golgi membrane.

Gene expression databases

BgeeiENSBTAG00000013971.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 5 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPI4KA_BOVIN
AccessioniPrimary (citable) accession number: O02811
Secondary accession number(s): F1MLV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: February 17, 2016
Last modified: November 30, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.