Reviewed, UniProtKB/Swiss-Prot O02810 (PI4KB_BOVIN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase beta
      Short name=PtdIns 4-kinase beta
      Short name=PI4K-beta
      Short name=PI4Kbeta
    EC=2.7.1.67

Gene names

Name:	PI4KB
Synonyms:	PIK4CB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation By similarity. Involved in Golgi-to-plasma membrane trafficking By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate. Ref.1
Enzyme regulation	Inhibited by wortmannin. Increased kinase acitvity upon interaction with FREQ/NCS1. Ref.1 Ref.2
Subunit structure	Interacts with myristoylated FREQ/NCS1 in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner By similarity. This binding competes with FREQ/NCS1 binding By similarity. Interacts with ARF1 and ARF3 By similarity.
Subcellular location	Endomembrane system By similarity. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Rough endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Golgi apparatus. Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: O02810-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: O02810-2)

The sequence of this isoform differs from the canonical sequence as follows:
     304-318: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 816	816	Phosphatidylinositol 4-kinase beta		PRO_0000088828
Regions
	Domain	543 – 750	208	PI3K/PI4K
Amino acid modifications
	Modified residue	258	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	263	1	Phosphothreonine By similarity UniProtKB Q9UBF8
	Modified residue	266	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	277	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	294	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	428	1	Phosphoserine By similarity
	Modified residue	438	1	Phosphothreonine By similarity UniProtKB Q9UBF8
	Modified residue	511	1	Phosphoserine By similarity UniProtKB Q9UBF8
	Modified residue	517	1	Phosphothreonine By similarity
	Modified residue	519	1	Phosphothreonine By similarity UniProtKB Q9UBF8
Natural variations
	Alternative sequence	304 – 318	15	Missing in isoform 2. Ref.1		VSP_050628

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 14, 2003. Version 2. Checksum: 4388D127636B500A	FASTA	816	91,375
	10 20 30 40 50 60 MGDTIVEPAP LKPTSEPAPG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK 70 80 90 100 110 120 LLHGGVAISS RGTPLELVNG DGVDSEIRCL DDPPAQIREE EDEMGATVAS GTAKGARRRR 130 140 150 160 170 180 QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL 190 200 210 220 230 240 NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 250 260 270 280 290 300 LSDELKPAHR KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 310 320 330 340 350 360 EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ RLISELSLLN 370 380 390 400 410 420 HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY VEVLECENFD TTSVPARIPE 430 440 450 460 470 480 NRIRSTRSVE NLPECGITHE QRAGSFSTVP NYDNDDEAWS VDDIGELQVE LPEVHTNSCD 490 500 510 520 530 540 NISQFSVDSI TSQESKEPVF IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK 550 560 570 580 590 600 VRRIREGSPY GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI 610 620 630 640 650 660 LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ RNFVQSCAGY 670 680 690 700 710 720 CLGCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN LGFETSAFKL TTEFVDVMGG 730 740 750 760 770 780 LDGDMFNYYK MLMLQGLIAA RKHMDKVVQI VEIMQQGSQL PCFHGSSTIR NLKERFHMSM 790 800 810 TEEQLQLLVE QMVDGSMRSI TTKLYDGFQY LTNGIM
	Isoform 2. Checksum: 8B6B0D34450ED08C	FASTA	801	89,803
	10 20 30 40 50 60 MGDTIVEPAP LKPTSEPAPG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK 70 80 90 100 110 120 LLHGGVAISS RGTPLELVNG DGVDSEIRCL DDPPAQIREE EDEMGATVAS GTAKGARRRR 130 140 150 160 170 180 QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL 190 200 210 220 230 240 NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 250 260 270 280 290 300 LSDELKPAHR KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 310 320 330 340 350 360 EDEPVRLAPE REFIKSLMAI GKRLATLPTK EQKTQRLISE LSLLNHKLPA RVWLPTAGFD 370 380 390 400 410 420 HHVVRVPHTQ AVVLNSKDKA PYLIYVEVLE CENFDTTSVP ARIPENRIRS TRSVENLPEC 430 440 450 460 470 480 GITHEQRAGS FSTVPNYDND DEAWSVDDIG ELQVELPEVH TNSCDNISQF SVDSITSQES 490 500 510 520 530 540 KEPVFIAAGD IRRRLSEQLA HTPTAFKRDP EDPSAVALKE PWQEKVRRIR EGSPYGHLPN 550 560 570 580 590 600 WRLLSVIVKC GDDLRQELLA FQVLKQLQSI WEQERVPLWI KPYKILVISA DSGMIEPVVN 610 620 630 640 650 660 AVSIHQVKKQ SQLSLLDYFL QEHGSYTTEA FLSAQRNFVQ SCAGYCLGCY LLQVKDRHNG 670 680 690 700 710 720 NILLDAEGHI IHIDFGFILS SSPRNLGFET SAFKLTTEFV DVMGGLDGDM FNYYKMLMLQ 730 740 750 760 770 780 GLIAARKHMD KVVQIVEIMQ QGSQLPCFHG SSTIRNLKER FHMSMTEEQL QLLVEQMVDG 790 800 SMRSITTKLY DGFQYLTNGI M

References

[1]

"Isolation and molecular cloning of wortmannin-sensitive bovine type III phosphatidylinositol 4-kinases." Balla T., Downing G.J., Jaffe H., Kim S., Zolyomi A., Catt K.J. J. Biol. Chem. 272:18358-18366(1997) [PubMed: 9218477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 153-158; 298-303; 319-321; 327-330; 339-345; 497-507; 510-522 AND 581-589, FUNCTION, ENZYME REGULATION. Tissue: Adrenal cortex.

[2]

"Interaction of neuronal calcium sensor-1 (NCS-1) with phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and affects membrane trafficking in COS-7 cells." Zhao X., Varnai P., Tuymetova G., Balla A., Toth Z.E., Oker-Blom C., Roder J., Jeromin A., Balla T. J. Biol. Chem. 276:40183-40189(2001) [PubMed: 11526106] [Abstract] Cited for: INTERACTION WITH FREQ, ENZYME REGULATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U88531 mRNA. Translation: AAC48729.1.
IPI	IPI00689037. IPI00693481.
RefSeq	NP_777208.1.
UniGene	Bt.688
3D structure databases
HSSP	HSSP built from PDB template 1E8Y based on UniProtKB P48736.
ModBase	Search...
Protein-protein interaction databases
STRING	O02810.
Genome annotation databases
Ensembl	ENSBTAT00000009627; ENSBTAP00000009627; ENSBTAG00000007320; Bos taurus. [Genome view] ENSBTAT00000009628; ENSBTAP00000009628; ENSBTAG00000007320; Bos taurus. [Genome view]
GeneID	286846.
KEGG	bta:286846.
Organism-specific databases
CTD	286846.
Phylogenomic databases
HOVERGEN	O02810.
Enzyme and pathway databases
BRENDA	2.7.1.67. 251.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI4KB_BOVIN

Accession

Primary (citable) accession number: O02810

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	November 14, 2003
Last modified:	October 13, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)