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Protein

DNA nucleotidylexotransferase

Gene

DNTT

Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).By similarity

Cofactori

Mg2+By similarityNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi345MagnesiumBy similarity1
Metal bindingi347MagnesiumBy similarity1
Metal bindingi442MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Terminal addition

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.31)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name:
TDT
Short name:
Terminal transferase
Gene namesi
Name:DNTT
Synonyms:TDT
OrganismiMonodelphis domestica (Gray short-tailed opossum)
Taxonomic identifieri13616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
Proteomesi
  • UP000002280 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187931 – 518DNA nucleotidylexotransferaseAdd BLAST518

Interactioni

Subunit structurei

Interacts with PRP19 and DNTTIP1. Interacts with TRERF1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA (By similarity).By similarity

Protein-protein interaction databases

STRINGi13616.ENSMODP00000005738.

Structurei

3D structure databases

ProteinModelPortaliO02789.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 124BRCTPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 518Mediates interaction with DNTTIP2By similarityAdd BLAST366
Regioni260 – 264Involved in DNA bindingBy similarity5
Regioni335 – 340Deoxynucleoside triphosphate bindingBy similarity6
Regioni344 – 347Deoxynucleoside triphosphate bindingBy similarity4
Regioni457 – 458Deoxynucleoside triphosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiO02789.
KOiK00977.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O02789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRIRTIDSD FGKKRQKKMD NHISSMIYEI KFHEFVLFIL EKKMGATRRT
60 70 80 90 100
FLTDLARKKG FRVENELSNS VTHIVAENNS GSDVLAWLKT HKMEKTTQFE
110 120 130 140 150
LLDISWLIEC MKVGKPVDTK GKYQLMESRV DSANPDPTAG TLNILPPTTK
160 170 180 190 200
TISQYACQRR TTINNHNQRF TDAFEILAKN YEFKENDDTC LTFMRAISVL
210 220 230 240 250
KCLPFEVVSL KDTEGLPWIG DEVKGIMEEI IEDGESLEVQ AVLNDERYQS
260 270 280 290 300
FKLFTSVFGV GLKTADKWYR MGFRTLNKIR SDKTLKLTKM QKAGLCYYED
310 320 330 340 350
LIDCVSKAEA DAVSLLVQDA VWTFLPDALV TITGGFRRGK EFGHDVDFLI
360 370 380 390 400
TSPGAEKEQE DQLLQKVTNL WKKQGLLLYC DLIESTFEDL KLPSRKIDAL
410 420 430 440 450
DHFQKCFLIL KLYHHKEDKR KWEMPTGSNE SEAKSWKAIR VDLVVCPYDR
460 470 480 490 500
YAFALLGWSG SRQFERDLRR YATHEKKMML DNHALYDKTK KIFLKAKSEE
510
EIFAHLGLEY IQPSERNA
Length:518
Mass (Da):60,010
Last modified:July 1, 1997 - v1
Checksum:i28E043428FEF3C42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91571 mRNA. Translation: AAC17587.1.
RefSeqiNP_001028151.1. NM_001032979.1.
UniGeneiMdm.235.

Genome annotation databases

GeneIDi554183.
KEGGimdo:554183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91571 mRNA. Translation: AAC17587.1.
RefSeqiNP_001028151.1. NM_001032979.1.
UniGeneiMdm.235.

3D structure databases

ProteinModelPortaliO02789.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi13616.ENSMODP00000005738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi554183.
KEGGimdo:554183.

Organism-specific databases

CTDi1791.

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiO02789.
KOiK00977.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTDT_MONDO
AccessioniPrimary (citable) accession number: O02789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.