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Protein

DNA nucleotidylexotransferase

Gene

DNTT

Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).By similarity

Cofactori

Mg2+By similarityNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi345MagnesiumBy similarity1
Metal bindingi347MagnesiumBy similarity1
Metal bindingi442MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTerminal addition
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.31)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name:
TDT
Short name:
Terminal transferase
Gene namesi
Name:DNTT
Synonyms:TDT
OrganismiMonodelphis domestica (Gray short-tailed opossum)
Taxonomic identifieri13616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
Proteomesi
  • UP000002280 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187931 – 518DNA nucleotidylexotransferaseAdd BLAST518

Proteomic databases

PRIDEiO02789

Interactioni

Subunit structurei

Interacts with PRP19 and DNTTIP1. Interacts with TRERF1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA (By similarity).By similarity

Protein-protein interaction databases

STRINGi13616.ENSMODP00000005738

Structurei

3D structure databases

ProteinModelPortaliO02789
SMRiO02789
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 124BRCTPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 518Mediates interaction with DNTTIP2By similarityAdd BLAST366
Regioni260 – 264Involved in DNA bindingBy similarity5
Regioni335 – 340Deoxynucleoside triphosphate bindingBy similarity6
Regioni344 – 347Deoxynucleoside triphosphate bindingBy similarity4
Regioni457 – 458Deoxynucleoside triphosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated

Phylogenomic databases

eggNOGiKOG2534 Eukaryota
COG1796 LUCA
HOGENOMiHOG000263600
HOVERGENiHBG003670
InParanoidiO02789
KOiK00977

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR018944 DNA_pol_lambd_fingers_domain
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR029398 PolB_thumb
IPR027292 TdT
IPR001726 TdT/Mu
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF10391 DNA_pol_lambd_f, 1 hit
PF14716 HHH_8, 1 hit
PIRSFiPIRSF000817 DNA_NT, 1 hit
PIRSF501175 TDT, 1 hit
PRINTSiPR00869 DNAPOLX
PR00871 DNAPOLXTDT
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS00522 DNA_POLYMERASE_X, 1 hit

Sequencei

Sequence statusi: Complete.

O02789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRIRTIDSD FGKKRQKKMD NHISSMIYEI KFHEFVLFIL EKKMGATRRT
60 70 80 90 100
FLTDLARKKG FRVENELSNS VTHIVAENNS GSDVLAWLKT HKMEKTTQFE
110 120 130 140 150
LLDISWLIEC MKVGKPVDTK GKYQLMESRV DSANPDPTAG TLNILPPTTK
160 170 180 190 200
TISQYACQRR TTINNHNQRF TDAFEILAKN YEFKENDDTC LTFMRAISVL
210 220 230 240 250
KCLPFEVVSL KDTEGLPWIG DEVKGIMEEI IEDGESLEVQ AVLNDERYQS
260 270 280 290 300
FKLFTSVFGV GLKTADKWYR MGFRTLNKIR SDKTLKLTKM QKAGLCYYED
310 320 330 340 350
LIDCVSKAEA DAVSLLVQDA VWTFLPDALV TITGGFRRGK EFGHDVDFLI
360 370 380 390 400
TSPGAEKEQE DQLLQKVTNL WKKQGLLLYC DLIESTFEDL KLPSRKIDAL
410 420 430 440 450
DHFQKCFLIL KLYHHKEDKR KWEMPTGSNE SEAKSWKAIR VDLVVCPYDR
460 470 480 490 500
YAFALLGWSG SRQFERDLRR YATHEKKMML DNHALYDKTK KIFLKAKSEE
510
EIFAHLGLEY IQPSERNA
Length:518
Mass (Da):60,010
Last modified:July 1, 1997 - v1
Checksum:i28E043428FEF3C42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91571 mRNA Translation: AAC17587.1
RefSeqiNP_001028151.1, NM_001032979.1
UniGeneiMdm.235

Genome annotation databases

GeneIDi554183
KEGGimdo:554183

Similar proteinsi

Entry informationi

Entry nameiTDT_MONDO
AccessioniPrimary (citable) accession number: O02789
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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