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Protein

Serotonin N-acetyltransferase

Gene

AANAT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.1 Publication

Catalytic activityi

Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine.1 Publication

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetylserotonin O-methyltransferase (ASMT), Serotonin N-acetyltransferase (AANAT)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120Important for the catalytic mechanism; involved in substrate deprotonationBy similarity1
Sitei122Important for the catalytic mechanism; involved in substrate deprotonationBy similarity1
Binding sitei124Substrate; via amide nitrogenBy similarity1
Binding sitei159Substrate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Biological rhythms, Melatonin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00837; UER00815.

Names & Taxonomyi

Protein namesi
Recommended name:
Serotonin N-acetyltransferase (EC:2.3.1.871 Publication)
Short name:
Serotonin acetylase
Alternative name(s):
Aralkylamine N-acetyltransferase
Short name:
AA-NAT
Gene namesi
Name:AANAT
Synonyms:SNAT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745791 – 207Serotonin N-acetyltransferaseAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31Phosphothreonine; by PKABy similarity1
Modified residuei205PhosphoserineBy similarity1

Post-translational modificationi

cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO02785.
PRIDEiO02785.

Expressioni

Tissue specificityi

High levels in pineal gland and retina.1 Publication

Inductioni

Exhibits night/day variations with an increased expression at night. Higher levels in pineal gland in early morning than in early afternoon (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with several 14-3-3 proteins, including YWHAB, YWHAE, YWHAG and YWHAZ, preferentially when phosphorylated at Thr-31 (By similarity). Phosphorylation on Ser-205 also allows binding to YWHAZ, but with lower affinity (By similarity). The interaction with YWHAZ considerably increases affinity for arylalkylamines and acetyl-CoA and protects the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019543.

Structurei

3D structure databases

ProteinModelPortaliO02785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 196N-acetyltransferasePROSITE-ProRule annotationAdd BLAST162

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 126Acetyl-CoA bindingBy similarity3
Regioni132 – 137Acetyl-CoA bindingBy similarity6
Regioni168 – 170Acetyl-CoA bindingBy similarity3

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4144. Eukaryota.
COG0454. LUCA.
HOGENOMiHOG000115812.
HOVERGENiHBG016332.
InParanoidiO02785.
KOiK00669.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O02785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPSIHCLK PSPLHLPSGI PGSPGRQRRH TLPANEFRCL TPKDAAGVFE
60 70 80 90 100
IEREAFISVS GNCPLNLDEV RHFLTLCPEL SLGWFVEGRL VAFIIGSLWD
110 120 130 140 150
EERLTQESLT LHRPGGRTAH LHALAVHHSF RQQGKGSVLL WRYLQHAGGQ
160 170 180 190 200
PAVRRAVLMC EDALVPFYQR FGFHPAGPCA VVVGSLTFTE MHCSLRGHAA

LRRNSDR
Length:207
Mass (Da):22,997
Last modified:July 1, 1997 - v1
Checksum:i734990F5CED18695
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AD000742 mRNA. Translation: AAB58942.1.
RefSeqiNP_803475.1. NM_177509.2.
UniGeneiBt.13120.

Genome annotation databases

GeneIDi281583.
KEGGibta:281583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AD000742 mRNA. Translation: AAB58942.1.
RefSeqiNP_803475.1. NM_177509.2.
UniGeneiBt.13120.

3D structure databases

ProteinModelPortaliO02785.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019543.

Proteomic databases

PaxDbiO02785.
PRIDEiO02785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281583.
KEGGibta:281583.

Organism-specific databases

CTDi15.

Phylogenomic databases

eggNOGiKOG4144. Eukaryota.
COG0454. LUCA.
HOGENOMiHOG000115812.
HOVERGENiHBG016332.
InParanoidiO02785.
KOiK00669.

Enzyme and pathway databases

UniPathwayiUPA00837; UER00815.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNAT_BOVIN
AccessioniPrimary (citable) accession number: O02785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.