Reviewed, UniProtKB/Swiss-Prot O02776 (PARG_BOVIN)

Names and origin

Protein names

Recommended name:
    Poly(ADP-ribose) glycohydrolase
    EC=3.2.1.143

Gene names

Name:

PARG

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	977 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells.
Catalytic activity	Hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the poly(ADP-ribose) glycohydrolase family.

Ontologies

Keywords
Cellular component	Nucleus
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(ADP-ribose) glycohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 977	977	Poly(ADP-ribose) glycohydrolase		PRO_0000066601
Regions
	Motif	10 – 16	7	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	138	1	Phosphoserine By similarity
	Modified residue	198	1	Phosphoserine By similarity
	Modified residue	200	1	Phosphothreonine By similarity
	Modified residue	262	1	Phosphoserine By similarity
	Modified residue	265	1	Phosphoserine By similarity
	Modified residue	317	1	Phosphoserine By similarity
	Modified residue	504	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02776-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 87D2100F979DF377	FASTA	977	110,837
	10 20 30 40 50 60 MSAGPGCEPC TKRPRWDAAA TSPPAASDAR SFPGRQRRVL DSKDAPVQFR VPPSSSGCAL 70 80 90 100 110 120 GRAGQHRGSA TSLVFKQKTI TSWMDTKGIK TVESESLHSK ENNNTREESM MSSVQKDNFY 130 140 150 160 170 180 QHNMEKLENV SQLGFDKSPV EKGTQYLKQH QTAAMCKWQN EGPHSERLLE SEPPAVTLVP 190 200 210 220 230 240 EQFSNANVDQ SSPKDDHSDT NSEESRDNQQ FLTHVKLANA KQTMEDEQGR EARSHQKCGK 250 260 270 280 290 300 ACHPAEACAG CQQEETDVVS ESPLSDTGSE DVGTGLKNAN RLNRQESSLG NSPPFEKESE 310 320 330 340 350 360 PESPMDVDNS KNSCQDSEAD EETSPGFDEQ EDSSSAQTAN KPSRFQPREA DTELRKRSSA 370 380 390 400 410 420 KGGEIRLHFQ FEGGESRAGM NDVNAKRPGS TSSLNVECRN SKQHGRKDSK ITDHFMRVPK 430 440 450 460 470 480 AEDKRKEQCE MKHQRTERKI PKYIPPHLSP DKKWLGTPIE EMRRMPRCGI RLPPLRPSAN 490 500 510 520 530 540 HTVTIRVDLL RIGEVPKPFP THFKDLWDNK HVKMPCSEQN LYPVEDENGE RAAGSRWELI 550 560 570 580 590 600 QTALLNRLTR PQNLKDAILK YNVAYSKKWD FTALIDFWDK VLEEAEAQHL YQSILPDMVK 610 620 630 640 650 660 IALCLPNICT QPIPLLKQKM NHSITMSQEQ IASLLANAFF CTFPRRNAKM KSEYSSYPDI 670 680 690 700 710 720 NFNRLFEGRS SRKPEKLKTL FCYFRRVTEK KPTGLVTFTR QSLEDFPEWE RCEKLLTRLH 730 740 750 760 770 780 VTYEGTIEGN GQGMLQVDFA NRFVGGGVTS AGLVQEEIRF LINPELIVSR LFTEVLDHNE 790 800 810 820 830 840 CLIITGTEQY SEYTGYAETY RWARSHEDRS ERDDWQRRTT EIVAIDALHF RRYLDQFVPE 850 860 870 880 890 900 KIRRELNKAY CGFLRPGVSS ENLSAVATGN WGCGAFGGDA RLKALIQILA AAVAERDVVY 910 920 930 940 950 960 FTFGDSELMR DIYSMHTFLT ERKLTVGEVY KLLLRYYNEE CRNCSTPGPD IKLYPFIYHA 970 VESCTQTTNQ PGQRTGA

References

[1]

"Isolation and characterization of the cDNA encoding bovine poly(ADP-ribose) glycohydrolase." Lin W., Ame J.-C., Aboul-Ela N., Jacobson E.L., Jacobson M.K. J. Biol. Chem. 272:11895-11901(1997) [PubMed: 9115250] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 601-616; 760-801 AND 849-877. Tissue: Thymus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U78975 mRNA. Translation: AAB53370.1.
IPI	IPI00700481.
RefSeq	NP_776563.1.
UniGene	Bt.65122
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O02776.
Genome annotation databases
Ensembl	ENSBTAT00000031269; ENSBTAP00000031225; ENSBTAG00000023018; Bos taurus. [Genome view]
GeneID	281377.
KEGG	bta:281377.
Organism-specific databases
CTD	281377.
Phylogenomic databases
HOVERGEN	O02776.
Enzyme and pathway databases
BRENDA	3.2.1.143. 251.
Family and domain databases
InterPro	IPR007724. Poly_GlycHdrlase. [Graphical view]
PANTHER	PTHR12837. Poly_glchydro. 1 hit.
Pfam	PF05028. PARG_cat. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARG_BOVIN

Accession

Primary (citable) accession number: O02776

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)