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O02773

- MA1A1_PIG

UniProt

O02773 - MA1A1_PIG

Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA

Gene

MAN1A1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Calcium.By similarity

    Enzyme regulationi

    Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi639 – 6391CalciumBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH47. Glycoside Hydrolase Family 47.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113)
    Alternative name(s):
    Man(9)-alpha-mannosidase
    Short name:
    Man9-mannosidase
    Mannosidase alpha class 1A member 1
    Processing alpha-1,2-mannosidase IA
    Short name:
    Alpha-1,2-mannosidase IA
    Gene namesi
    Name:MAN1A1
    Synonyms:MAN1A
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass type II membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 659659Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAPRO_0000210310Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi482 ↔ 514By similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000004583.

    Structurei

    3D structure databases

    ProteinModelPortaliO02773.
    SMRiO02773. Positions 182-647.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4848CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini70 – 659590LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei49 – 6921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300315.
    HOGENOMiHOG000181988.
    HOVERGENiHBG052389.
    KOiK01230.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O02773-1 [UniParc]FASTAAdd to Basket

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    MPVGGLLPLF SSPAGGGLGG GLGGGLGGGG GGGGRKGSGP SAFRLTEKFV    50
    LLLVFSAFIT LCFGAIFFLP DSSKLLSGVL FHSSPALQPA ADHKPGPGAR 100
    AEDAADGRAR PGEEGAPGDP AAALEDNLAR IRENHERALM EAKETLQKLP 150
    EEIQRDILME KEKVAQDQMS NRMGFRLPPV YLVPLIGAID REPADAAVRE 200
    KRAKIKEMMK HAWNNYKLYA WGKNELKPVS KGGHSSSLFG NIKGATIVDA 250
    LDTLFIMKMK NEFEEAKAWV EEHLNFNVNA EVSVFEVNIR FIGGLISAYY 300
    LSGEEIFRKK AVELGVKLLP AFYTPSGIPW ALLNIKSGIG RNWPWASGGS 350
    SILAEFGTLH LEFIHLSYLS GNPFFAEKVM NIRKVLNNLE KPQGLYPNYL 400
    NPNSGQWGQY HVSVGGLGDS FYEYLLKAWL MSDKTDLEAK KMYFDAIKAI 450
    ETHLIRKSRN GLTYIAEWKG GLLEHKMGHL TCFAGGMFAL GADDAPDGLT 500
    QHYLQLGAEI ARTCHESYSR TFVKLGPEAF RFDGGVEAIA TRQNEKYYIL 550
    RPEVVETYLY MWRLTHDPKY RKWAWEAVEA LEKHCRVNGG YSGLRDVYVS 600
    AQTYDDVQQS FFLAETLKYL YLIFSDDDLL PLEHWIFNTE AHPLPVLSRN 650
    IKKVEDNEK 659
    Length:659
    Mass (Da):73,197
    Last modified:July 1, 1997 - v1
    Checksum:iAA7F17FEAAFE43A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y12503 mRNA. Translation: CAA73105.1.
    PIRiS78554.
    RefSeqiNP_999050.1. NM_213885.1.
    UniGeneiSsc.14512.

    Genome annotation databases

    GeneIDi396919.
    KEGGissc:396919.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y12503 mRNA. Translation: CAA73105.1 .
    PIRi S78554.
    RefSeqi NP_999050.1. NM_213885.1.
    UniGenei Ssc.14512.

    3D structure databases

    ProteinModelPortali O02773.
    SMRi O02773. Positions 182-647.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000004583.

    Protein family/group databases

    CAZyi GH47. Glycoside Hydrolase Family 47.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396919.
    KEGGi ssc:396919.

    Organism-specific databases

    CTDi 4121.

    Phylogenomic databases

    eggNOGi NOG300315.
    HOGENOMi HOG000181988.
    HOVERGENi HBG052389.
    KOi K01230.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Man9-mannosidase from pig liver is a type-II membrane protein that resides in the endoplasmic reticulum. cDNA cloning and expression of the enzyme in COS 1 cells."
      Bieberich E., Treml K., Volker C., Rolfs A., Kalz-Fueller B., Bause E.
      Eur. J. Biochem. 246:681-689(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION.
      Tissue: Liver.
    2. "Molecular cloning and primary structure of Man9-mannosidase from human kidney."
      Bause E., Bieberich E., Rolfs A., Voelker C., Schmidt B.
      Eur. J. Biochem. 217:535-540(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiMA1A1_PIG
    AccessioniPrimary (citable) accession number: O02773
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3