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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA

Gene

MAN1A1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Enzyme regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei528Proton donorBy similarity1
Metal bindingi639CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113By similarity)
Alternative name(s):
Man(9)-alpha-mannosidase
Short name:
Man9-mannosidase
Mannosidase alpha class 1A member 1
Processing alpha-1,2-mannosidase IA
Short name:
Alpha-1,2-mannosidase IA
Gene namesi
Name:MAN1A1
Synonyms:MAN1A
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicSequence analysisAdd BLAST48
Transmembranei49 – 69Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini70 – 659LumenalSequence analysisAdd BLAST590

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103101 – 659Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAAdd BLAST659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi482 ↔ 514By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO02773
PeptideAtlasiO02773
PRIDEiO02773

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000004583

Structurei

3D structure databases

ProteinModelPortaliO02773
SMRiO02773
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
HOGENOMiHOG000181988
HOVERGENiHBG052389
InParanoidiO02773
KOiK01230

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

O02773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVGGLLPLF SSPAGGGLGG GLGGGLGGGG GGGGRKGSGP SAFRLTEKFV
60 70 80 90 100
LLLVFSAFIT LCFGAIFFLP DSSKLLSGVL FHSSPALQPA ADHKPGPGAR
110 120 130 140 150
AEDAADGRAR PGEEGAPGDP AAALEDNLAR IRENHERALM EAKETLQKLP
160 170 180 190 200
EEIQRDILME KEKVAQDQMS NRMGFRLPPV YLVPLIGAID REPADAAVRE
210 220 230 240 250
KRAKIKEMMK HAWNNYKLYA WGKNELKPVS KGGHSSSLFG NIKGATIVDA
260 270 280 290 300
LDTLFIMKMK NEFEEAKAWV EEHLNFNVNA EVSVFEVNIR FIGGLISAYY
310 320 330 340 350
LSGEEIFRKK AVELGVKLLP AFYTPSGIPW ALLNIKSGIG RNWPWASGGS
360 370 380 390 400
SILAEFGTLH LEFIHLSYLS GNPFFAEKVM NIRKVLNNLE KPQGLYPNYL
410 420 430 440 450
NPNSGQWGQY HVSVGGLGDS FYEYLLKAWL MSDKTDLEAK KMYFDAIKAI
460 470 480 490 500
ETHLIRKSRN GLTYIAEWKG GLLEHKMGHL TCFAGGMFAL GADDAPDGLT
510 520 530 540 550
QHYLQLGAEI ARTCHESYSR TFVKLGPEAF RFDGGVEAIA TRQNEKYYIL
560 570 580 590 600
RPEVVETYLY MWRLTHDPKY RKWAWEAVEA LEKHCRVNGG YSGLRDVYVS
610 620 630 640 650
AQTYDDVQQS FFLAETLKYL YLIFSDDDLL PLEHWIFNTE AHPLPVLSRN

IKKVEDNEK
Length:659
Mass (Da):73,197
Last modified:July 1, 1997 - v1
Checksum:iAA7F17FEAAFE43A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12503 mRNA Translation: CAA73105.1
PIRiS78554
RefSeqiNP_999050.1, NM_213885.1
UniGeneiSsc.14512

Genome annotation databases

GeneIDi396919
KEGGissc:396919

Similar proteinsi

Entry informationi

Entry nameiMA1A1_PIG
AccessioniPrimary (citable) accession number: O02773
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: July 1, 1997
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health