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Protein

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

Gene

CYP8B1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in bile acid synthesis and is responsible for the conversion of 7-alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.2 Publications

Catalytic activityi

7-alpha-hydroxycholest-4-en-3-one + 2 ferrocytochrome b5 + 2 H+ + O2 = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + 2 ferricytochrome b5 + + H2O.2 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi439Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14303.
SABIO-RKO02766.

Chemistry databases

SwissLipidsiSLP:000001318.

Names & Taxonomyi

Protein namesi
Recommended name:
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (EC:1.14.18.82 Publications)
Alternative name(s):
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase1 Publication
CYPVIIIB1
Cytochrome P450 8B1
Sterol 12-alpha-hydroxylase
Gene namesi
Name:CYP8B1
Synonyms:CYP12
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2 – 21HelicalSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000519152 – 5007-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylaseAdd BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei325PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Liver.

Inductioni

By starvation.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000023170.

Structurei

3D structure databases

ProteinModelPortaliO02766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiO02766.
KOiK07431.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR030686. Cytochrome_CYP8B1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500627. Cytochrome_CYP8B1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O02766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLWGLLGAL LMVMVGWLCL PGLLRQRRPQ EPPLDKGSIP WLGHAMTFRK
60 70 80 90 100
NMLEFLKHMR SKHGDVFTVQ LGGQYFTFVM DPVSFGPILK DGQRKLDFVE
110 120 130 140 150
YAKGLVLKVF GYQSIEGDHR MIHLASTKHL MGHGLEELNK AMLDSLSLVM
160 170 180 190 200
LGPEGRSPDA SRWHEDGLFH FCYGVMFKAG YLSLFGHTSD KRQDLLQAEE
210 220 230 240 250
IFIKFRRFDL LFPRFVYSLL GPREWREVGR LQQLFHELLS VKHNPEKDGM
260 270 280 290 300
SNWIGHMLQY LSEQGVAPAM QDKFNFMMLW ASQGNTGPAS FWALIYLLKH
310 320 330 340 350
PEAMRAVKEE ATRVLGEPRL EAKQSFTVQL SALQHIPVLD SVMEETLRLG
360 370 380 390 400
AAPTLYRVVQ KDILLKMASG QECLLRQGDI VTLFPYLSVH MDPDIHPEPT
410 420 430 440 450
TFKYDRFLNP NGSRKVDFYK AGQKIHHYTM PWGSGVSICP GRFFALSEMK
460 470 480 490 500
LFVLLMVQYF DLELVDPNTP VPPIDPRRWG FGTMQPTHDV RIRYRLKPLE
Length:500
Mass (Da):57,494
Last modified:January 23, 2007 - v3
Checksum:i4FE56CBB724F751E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08269 mRNA. Translation: CAA69594.1.
PIRiA45103.
RefSeqiNP_001076091.1. NM_001082622.1.
UniGeneiOcu.3293.

Genome annotation databases

GeneIDi100009301.
KEGGiocu:100009301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08269 mRNA. Translation: CAA69594.1.
PIRiA45103.
RefSeqiNP_001076091.1. NM_001082622.1.
UniGeneiOcu.3293.

3D structure databases

ProteinModelPortaliO02766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000023170.

Chemistry databases

SwissLipidsiSLP:000001318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009301.
KEGGiocu:100009301.

Organism-specific databases

CTDi1582.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiO02766.
KOiK07431.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14303.
SABIO-RKO02766.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
IPR030686. Cytochrome_CYP8B1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500627. Cytochrome_CYP8B1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCP8B1_RABIT
AccessioniPrimary (citable) accession number: O02766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.