O02765 (TNFL4_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor ligand superfamily member 4

Alternative name(s):
OX40 ligand
Short name=OX40L
CD_antigen=CD252

Gene names

Name:	TNFSF4
Synonyms:	TXGP1

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Subunit structure	Homotrimer Probable.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T-helper 2 cell activation Inferred from electronic annotation. Source: Compara acute inflammatory response Inferred from electronic annotation. Source: Compara cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara cellular response to prostaglandin E stimulus Inferred from electronic annotation. Source: Compara chemokine (C-C motif) ligand 11 production Inferred from electronic annotation. Source: Compara cholesterol metabolic process Inferred from electronic annotation. Source: Compara defense response to nematode Inferred from electronic annotation. Source: Compara interleukin-4-dependent isotype switching to IgE isotypes Inferred from electronic annotation. Source: Compara memory T cell activation Inferred from electronic annotation. Source: Compara negative regulation of T-helper 1 cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of cytokine secretion Inferred from electronic annotation. Source: Compara negative regulation of interferon-gamma production Inferred from electronic annotation. Source: Compara negative regulation of interleukin-17 production Inferred from electronic annotation. Source: Compara negative regulation of regulatory T cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of B cell activation Inferred from electronic annotation. Source: Compara positive regulation of CD4-positive, alpha beta T cell costimulation Inferred from electronic annotation. Source: Compara positive regulation of T cell cytokine production Inferred from electronic annotation. Source: Compara positive regulation of T-helper 2 cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of alpha-beta T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of immunoglobulin mediated immune response Inferred from electronic annotation. Source: Compara positive regulation of immunoglobulin secretion Inferred from electronic annotation. Source: Compara positive regulation of inflammatory response Inferred from electronic annotation. Source: Compara positive regulation of interferon-gamma production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-10 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-12 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-13 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-4 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara positive regulation of memory T cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of type 2 immune response Inferred from electronic annotation. Source: Compara response to virus Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Tumor necrosis factor ligand superfamily member 4		PRO_0000185495
Regions
	Topological domain	1 – 23	23	Cytoplasmic Potential
	Transmembrane	24 – 44	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	45 – 187	143	Extracellular Potential
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	156	1	N-linked (GlcNAc...) Potential
	Disulfide bond	74 ↔ 164		By similarity
	Disulfide bond	101 ↔ 185		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02765 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: F19B1370E723D608	FASTA	187	20,886
	10 20 30 40 50 60 MEGVRPLEEN VGNAPRPRFE RNKLLLVASV VQALGLLLCL TYVCQHSHAP EVSLQYPPIE 70 80 90 100 110 120 NIMTQLQILT SHECEEDSFI LPLQKRDGTM EVQNNSVVIQ CDGFYLLSLK GYFSQEVSIS 130 140 150 160 170 180 LHYRKGEEPF PILKKTKFAN SNVVLKLGYK DKVYLNVTTD SASCKQLSVN AGELIVILQN PGGYCAP

References

[1]	"Expression of OX40 and OX40 ligand genes in rabbit HTLV-I-transformed T cell lines." Isono T., Seto A. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CHBB:HM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB003912 mRNA. Translation: BAA20060.1.
RefSeq	NP_001075454.1. NM_001081985.1.
UniGene	Ocu.2137.
3D structure databases
ProteinModelPortal	O02765.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000007420.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSOCUT00000008583; ENSOCUP00000007420; ENSOCUG00000008587.
GeneID	100008595.
Organism-specific databases
CTD	7292.
Phylogenomic databases
eggNOG	NOG47572.
GeneTree	ENSGT00390000015127.
HOGENOM	HOG000076309.
HOVERGEN	HBG056457.
OMA	MEGVQPL.
OrthoDB	EOG4K3KXF.
Family and domain databases
InterPro	IPR006052. TNF. IPR021184. TNF_CS. IPR008983. Tumour_necrosis_fac-like_dom. [Graphical view]
Pfam	PF00229. TNF. 1 hit. [Graphical view]
SMART	SM00207. TNF. 1 hit. [Graphical view]
SUPFAM	SSF49842. TNF_like. 1 hit.
PROSITE	PS00251. TNF_1. 1 hit. PS50049. TNF_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TNFL4_RABIT

Accession

Primary (citable) accession number: O02765

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program