Reviewed, UniProtKB/Swiss-Prot O02742 (ACK2_BOVIN)

Names and origin

Protein names

Recommended name:
    Activated CDC42 kinase 2
      Short name=ACK-2
    EC=2.7.10.2

Gene names

Name:

ACK2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tyrosine kinase, that after binding to CDC42, inhibits both its intrinsic and stimulated GTPase activity By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with CDC42. Ref.1
Tissue specificity	Expressed in brain and skeletal muscle. Weakly expressed in pancreas, heart, placenta and lung.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 CRIB domain. Contains 1 protein kinase domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC42	P60953	1	EBI-457220,EBI-81752	From a different organism.
CLTC	Q00610	1	EBI-457220,EBI-354967	From a different organism.
SNX9	Q9Y5X1	2	EBI-457220,EBI-77848	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	Activated CDC42 kinase 2		PRO_0000088060
Regions
	Domain	126 – 385	260	Protein kinase
	Domain	386 – 448	63	SH3
	Domain	454 – 466	13	CRIB
	Nucleotide binding	132 – 140	9	ATP By similarity
	Compositional bias	517 – 647	131	Pro-rich
Sites
	Active site	252	1	Proton acceptor By similarity
	Binding site	158	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02742-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7A5C7E9DF0DC06D8	FASTA	747	83,199
	10 20 30 40 50 60 MQPEEGTGWL LELLSEVQLQ QYFLRLRDDL NVTRLSHFEY VKNEDLEKIG MGRPGQRRLW 70 80 90 100 110 120 EAVKRRKAMC KRKSWMSKVF SGKRLEAEFP PHHSQSTFRK TSPTPGGSAG EGSLQSLTCL 130 140 150 160 170 180 IGEKDLHLFE KLGDGSFGVV RRGEWDAPSG KTVSVAVKCL KPDVLSQPEA MDDFIREVNA 190 200 210 220 230 240 MHSLDHRNLI RLYGVVLTPP MKMVTELAPL GSLLDRLRKH QGHFLLGTLS RYAVQVAEGM 250 260 270 280 290 300 GYLEAKRFIH RDLAARNLLL ATRDLVKIGD FGLMRALPQN DDHYVMQEHR KVPFAWCAPE 310 320 330 340 350 360 SLKTRTFSHA SDTWMFGVTL WEMFTYGQEP WIGLNGSQIL HKIDKEGERL PRPEDCPQDI 370 380 390 400 410 420 YNVMVQCWAH KPEDRPTFVA LRDFLLEAQP TDMRALQDFE EPDKLHIQMN DVITVIEGRA 430 440 450 460 470 480 ENYWWRGQNT RTLCVGPFPR NVVTSVAGLS AQDISQPLQN SFIHTGHGDS DPRHCWGFPD 490 500 510 520 530 540 KIDELYLGNP MDPPDLLSVE LSTSRPTQHL GRVKREPPPR PPQPAIFTQK PTYDPVSEDQ 550 560 570 580 590 600 DPLSSDFKKL GLRKPGLPRG LWLAKPSARV PGTKAGRGGG EVTLIDFGEE PVVPAPRPCA 610 620 630 640 650 660 PSLAQLAMDA CSLLDKTPPQ SPTRALPRPL HPTPVVDWDA RPLPPPPAYD DVAQDEDDFE 670 680 690 700 710 720 VCSINSTLVG AGVSAEPSQG ETNYAFVPEP ARLLPPAGGQ PVPPTPEWGQ ATQLGPNRRD 730 740 LPGAAAGMHA AATGPAWLSG PLTQPWG

References

[1]	"Cloning and characterization of a novel Cdc42-associated tyrosine kinase, ACK-2, from bovine brain." Yang W., Cerione R.A. J. Biol. Chem. 272:24819-24824(1997) [PubMed: 9312079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CDC42. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U96722 mRNA. Translation: AAC05310.1.
IPI	IPI00695698.
UniGene	Bt.64988
3D structure databases
HSSP	HSSP built from PDB template 1CF4 based on UniProtKB Q07912.
SMR	O02742. Positions 117-390.
ModBase	Search...
Protein-protein interaction databases
IntAct	O02742. 4 interactions.
Genome annotation databases
Ensembl	ENSBTAG00000021364. Bos taurus. [Contig view]
Phylogenomic databases
HOVERGEN	O02742.
Enzyme and pathway databases
BRENDA	2.7.10.2. 251.
Family and domain databases
InterPro	IPR015779. ACK_tyr_kin_N. IPR015116. GTPase_binding. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001452. SH3_domain. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
PANTHER	PTHR23256:SF265. ACK_tyr_kin_N. 1 hit.
Pfam	PF09027. GTPase_binding. 1 hit. PF07714. Pkinase_Tyr. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS50108. CRIB. False negative. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACK2_BOVIN

Accession

Primary (citable) accession number: O02742

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)