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Protein

Retinal guanylyl cyclase 2

Gene

GUCY2F

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction (By similarity).By similarity

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Enzyme regulationi

Activated by GCAP-1; inhibited by calcium.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cGMP biosynthesis, Sensory transduction, Vision

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Retinal guanylyl cyclase 2 (EC:4.6.1.2)
Short name:
RETGC-2
Alternative name(s):
Guanylate cyclase 2F, retinal
Guanylate cyclase F
Short name:
GC-F
Rod outer segment membrane guanylate cyclase 2
Short name:
ROS-GC2
Gene namesi
Name:GUCY2F
Synonyms:GUC2F
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini47 – 465ExtracellularSequence analysisAdd BLAST419
Transmembranei466 – 490HelicalSequence analysisAdd BLAST25
Topological domaini491 – 1103CytoplasmicSequence analysisAdd BLAST613

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 46By similarityAdd BLAST46
ChainiPRO_000001238547 – 1103Retinal guanylyl cyclase 2Add BLAST1057

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi104 ↔ 132By similarity
Disulfide bondi452InterchainCurated
Disulfide bondi460InterchainCurated

Post-translational modificationi

There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO02740.
PRIDEiO02740.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000036727.

Structurei

3D structure databases

ProteinModelPortaliO02740.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini532 – 812Protein kinasePROSITE-ProRule annotationAdd BLAST281
Domaini884 – 1014Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
HOGENOMiHOG000293307.
HOVERGENiHBG098487.
InParanoidiO02740.
KOiK12322.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR033484. GUCY2F.
IPR011645. HNOB_dom_associated.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PANTHERiPTHR11920:SF349. PTHR11920:SF349. 1 hit.
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O02740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAPWPFSH LMLWFVTLGR QRGQHGLASF KLLWCLWLLV LMSLPLQVWA
60 70 80 90 100
PPYKIGVVGP WTCDPLFSKA LPEIAAQLAT ERINKDPALD LGHSLEYVIF
110 120 130 140 150
NEDCQASRAL SSFISHHQMA SGFIGPANPG YCEAASLLGN SWDKGIFSWA
160 170 180 190 200
CVNYELDSKN SHPTFSRTLP SPIRVLLTVM KYFQWAHAGV ISSDEDIWVH
210 220 230 240 250
TAYRVASALR SRGLPVGVVL TTGQDSQSIQ KALQQIRQAD RIRIIIMCMH
260 270 280 290 300
STLIGGETQT HLLEWAHDLQ MTDGTYVFVP YDTLLYSLPY KHTPYKVLRN
310 320 330 340 350
NPKLREAYDA VLTITVESQE KTFYQAFEEA AARGEIPEKL ESDQVSPLFG
360 370 380 390 400
TIYNSIYFIA QAMNNAMKEN GWASAASLVQ HSRNVQFYGF NQLIRTDANG
410 420 430 440 450
NGISEYVILD TNWKEWELHS TYTVDMETEL LRFGETPIHF PGGRPPRADA
460 470 480 490 500
QCWFADGRIC QGGINPTFAL MVCLALLIAL LSINGFAYFI RHRINKIQLI
510 520 530 540 550
KGPNRILLTL EDVTFINPHF GSKRGSHASV SFQITSEVQS GRSPRLSFSS
560 570 580 590 600
GSLTPATCEN SNIAIYEGDW VWLKKFPSGN FGDIKSVESS ASDIFEMMKD
610 620 630 640 650
LRHENINPLV GFFYDSGVFA IVTEFCSRRS LEDILMNQDV KLDWMFKSSL
660 670 680 690 700
LLDLIKGMKY LHHREFAHGR LKSRNCVVDG RFVLKVTDYG FNDILETLRL
710 720 730 740 750
SQEEPSAEEL LWTAPELLRA PRGSRLRSFA GDVYSFAIIM QEVMVRGTPF
760 770 780 790 800
CMMDLPAKEI IERIKKPPPV YRPVVPPEHA PPECLQLMKQ CWAEAAEQRP
810 820 830 840 850
TFDEIFNQFK TFNKGKKTNI IDSMLRMLEQ YSSNLEDLIQ ERTEELEIEK
860 870 880 890 900
QKTEKLLTQM LPPSVAESLK KGCTVEPEGF DLVTLYFSDI VGFTTISAMS
910 920 930 940 950
EPIEVVDLLN DLYTLFDAII GSHDVYKVET IGDAYMVASG LPKRNGMRHA
960 970 980 990 1000
AEIANMSLDI LSSVGTFKMR HMPEVPVRIR IGLHSGPVVA GVVGLTMPRY
1010 1020 1030 1040 1050
CLFGDTVNTA SRMESTGLPY RIHVSHSTVT ILRTLGEGYE VELRGRTELK
1060 1070 1080 1090 1100
GKGTEETFWL VGKKGFTKPL PVPPPVGKDG QVGHGLQSVE IAAFQRRKQK

SSW
Length:1,103
Mass (Da):124,262
Last modified:July 1, 1997 - v1
Checksum:iEB731E1D8C642AA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95958 mRNA. Translation: AAB53864.1.
PIRiJC5581.
RefSeqiNP_776974.1. NM_174549.2.
UniGeneiBt.590.

Genome annotation databases

GeneIDi282246.
KEGGibta:282246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95958 mRNA. Translation: AAB53864.1.
PIRiJC5581.
RefSeqiNP_776974.1. NM_174549.2.
UniGeneiBt.590.

3D structure databases

ProteinModelPortaliO02740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000036727.

Proteomic databases

PaxDbiO02740.
PRIDEiO02740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282246.
KEGGibta:282246.

Organism-specific databases

CTDi2986.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
HOGENOMiHOG000293307.
HOVERGENiHBG098487.
InParanoidiO02740.
KOiK12322.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR033484. GUCY2F.
IPR011645. HNOB_dom_associated.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PANTHERiPTHR11920:SF349. PTHR11920:SF349. 1 hit.
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUC2F_BOVIN
AccessioniPrimary (citable) accession number: O02740
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.