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O02734 (HMCS2_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylglutaryl-CoA synthase, mitochondrial

Short name=HMG-CoA synthase
EC=2.3.3.10
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene names
Name:HMGCS2
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Catalytic activity

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the HMG-CoA synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3737Mitochondrion Probable
Chain38 – 508471Hydroxymethylglutaryl-CoA synthase, mitochondrial
PRO_0000013485

Sites

Active site1321Proton donor/acceptor By similarity
Active site1661Acyl-thioester intermediate By similarity
Active site3011Proton donor/acceptor By similarity
Binding site801Substrate By similarity
Binding site2041Substrate By similarity
Binding site2581Substrate By similarity
Binding site3801Substrate By similarity

Amino acid modifications

Modified residue521N6-succinyllysine By similarity
Modified residue2431N6-acetyllysine By similarity
Modified residue2561N6-acetyllysine; alternate By similarity
Modified residue2561N6-succinyllysine; alternate By similarity
Modified residue3061N6-acetyllysine By similarity
Modified residue3101N6-acetyllysine; alternate By similarity
Modified residue3101N6-succinyllysine; alternate By similarity
Modified residue3331N6-succinyllysine By similarity
Modified residue3421N6-acetyllysine; alternate By similarity
Modified residue3421N6-succinyllysine; alternate By similarity
Modified residue3501N6-acetyllysine; alternate By similarity
Modified residue3501N6-succinyllysine; alternate By similarity
Modified residue3541N6-acetyllysine; alternate By similarity
Modified residue3541N6-succinyllysine; alternate By similarity
Modified residue3581N6-acetyllysine; alternate By similarity
Modified residue3581N6-succinyllysine; alternate By similarity
Modified residue4371N6-acetyllysine By similarity
Modified residue4731N6-acetyllysine; alternate By similarity
Modified residue4731N6-succinyllysine; alternate By similarity

Sequences

Sequence LengthMass (Da)Tools
O02734 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 5479DE6F70B3C0F6

FASTA50856,934
        10         20         30         40         50         60 
MQRLLTPVRQ VLRVKRAMQE ASFMPPLLPP AAHQRFSTVP AVPVAKADTW PKDVGILALE 

        70         80         90        100        110        120 
VYFPAQYVDQ TDLEKFDNVE AGRYTVGLGQ THMGFCSVQE DINSLCLTVV QRLMERTQLP 

       130        140        150        160        170        180 
WDSVGWLEVG TETIIDKSKS VKTVLMELFQ DSGNTDIEGI DTTNACYGGT ASLFNAANWV 

       190        200        210        220        230        240 
ESSAWDGRYA VVVCGDIAVY PRGNSRPTGG AGAVAMLVGP EAPLALERGL RGTHMENAYD 

       250        260        270        280        290        300 
FYKPNATSEY PLVDGKLSIQ CYLRALDRCY TLYRQKIEKQ WKQAGIERHF TLDDLQFMIF 

       310        320        330        340        350        360 
HTPFCKLVQK SLARLMFSDF LLADSDTQSS LYKGLEAFRG QKLEDTYANK DIEKAFQKAS 

       370        380        390        400        410        420 
LDLFNKKTKP SLYLSLHNGN MYTSSLYGCL ASLLSQCSAQ DLAGSRIGAF SYGSGLAASF 

       430        440        450        460        470        480 
YSLRVSQDAS PGSPLEKLVS SVSDLPERLA SRKRVSPEEF TEIMNQREQY YHKVNFTPPG 

       490        500 
DPNSLFPGTW YLERVDELYR RKYARHLV 

« Hide

References

« Hide 'large scale' references
[1]"Gene expression of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase in a poorly ketogenic mammal: effect of starvation during the neonatal period of the piglet."
Adams S.H., Alho C.S., Asins G., Hegardt F.G., Marrero P.F.
Biochem. J. 324:65-73(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]Porcine genome sequencing project
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U90884 mRNA. Translation: AAC48727.1.
CR956647 Genomic DNA. Translation: CAN13229.1.
RefSeqNP_999545.1. NM_214380.2.
UniGeneSsc.14479.

3D structure databases

ProteinModelPortalO02734.
SMRO02734. Positions 53-505.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000007357; ENSSSCP00000007162; ENSSSCG00000006716.
GeneID397673.
KEGGssc:397673.

Organism-specific databases

CTD3158.

Phylogenomic databases

eggNOGCOG3425.
GeneTreeENSGT00390000006096.
HOGENOMHOG000012351.
HOVERGENHBG051912.
KOK01641.
OMAMLVGPEA.
OrthoDBEOG741Z1W.
TreeFamTF105361.

Enzyme and pathway databases

BRENDA2.3.3.10. 6170.
UniPathwayUPA00058; UER00102.

Family and domain databases

Gene3D3.40.47.10. 1 hit.
InterProIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 3 hits.
TIGRFAMsTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHMCS2_PIG
AccessionPrimary (citable) accession number: O02734
Secondary accession number(s): A5GFY7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways