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O02734

- HMCS2_PIG

UniProt

O02734 - HMCS2_PIG

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Protein

Hydroxymethylglutaryl-CoA synthase, mitochondrial

Gene

HMGCS2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801SubstrateBy similarity
Active sitei132 – 1321Proton donor/acceptorPROSITE-ProRule annotation
Active sitei166 – 1661Acyl-thioester intermediatePROSITE-ProRule annotation
Binding sitei204 – 2041SubstrateBy similarity
Binding sitei258 – 2581SubstrateBy similarity
Active sitei301 – 3011Proton donor/acceptorPROSITE-ProRule annotation
Binding sitei380 – 3801SubstrateBy similarity

GO - Molecular functioni

  1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. cholesterol biosynthetic process Source: UniProtKB-KW
  2. isoprenoid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.3.10. 6170.
ReactomeiREACT_226431. PPARA activates gene expression.
REACT_253457. Synthesis of Ketone Bodies.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase, mitochondrial (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:HMGCS2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 4

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737MitochondrionCuratedAdd
BLAST
Chaini38 – 508471Hydroxymethylglutaryl-CoA synthase, mitochondrialPRO_0000013485Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521N6-succinyllysineBy similarity
Modified residuei243 – 2431N6-acetyllysineBy similarity
Modified residuei256 – 2561N6-acetyllysine; alternateBy similarity
Modified residuei256 – 2561N6-succinyllysine; alternateBy similarity
Modified residuei306 – 3061N6-acetyllysineBy similarity
Modified residuei310 – 3101N6-acetyllysine; alternateBy similarity
Modified residuei310 – 3101N6-succinyllysine; alternateBy similarity
Modified residuei333 – 3331N6-succinyllysineBy similarity
Modified residuei342 – 3421N6-acetyllysine; alternateBy similarity
Modified residuei342 – 3421N6-succinyllysine; alternateBy similarity
Modified residuei350 – 3501N6-acetyllysine; alternateBy similarity
Modified residuei350 – 3501N6-succinyllysine; alternateBy similarity
Modified residuei354 – 3541N6-acetyllysine; alternateBy similarity
Modified residuei354 – 3541N6-succinyllysine; alternateBy similarity
Modified residuei358 – 3581N6-acetyllysine; alternateBy similarity
Modified residuei358 – 3581N6-succinyllysine; alternateBy similarity
Modified residuei437 – 4371N6-acetyllysineBy similarity
Modified residuei473 – 4731N6-acetyllysine; alternateBy similarity
Modified residuei473 – 4731N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO02734.
SMRiO02734. Positions 53-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3425.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiO02734.
KOiK01641.
OMAiMLVGPEA.
OrthoDBiEOG741Z1W.
TreeFamiTF105361.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O02734-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQRLLTPVRQ VLRVKRAMQE ASFMPPLLPP AAHQRFSTVP AVPVAKADTW
60 70 80 90 100
PKDVGILALE VYFPAQYVDQ TDLEKFDNVE AGRYTVGLGQ THMGFCSVQE
110 120 130 140 150
DINSLCLTVV QRLMERTQLP WDSVGWLEVG TETIIDKSKS VKTVLMELFQ
160 170 180 190 200
DSGNTDIEGI DTTNACYGGT ASLFNAANWV ESSAWDGRYA VVVCGDIAVY
210 220 230 240 250
PRGNSRPTGG AGAVAMLVGP EAPLALERGL RGTHMENAYD FYKPNATSEY
260 270 280 290 300
PLVDGKLSIQ CYLRALDRCY TLYRQKIEKQ WKQAGIERHF TLDDLQFMIF
310 320 330 340 350
HTPFCKLVQK SLARLMFSDF LLADSDTQSS LYKGLEAFRG QKLEDTYANK
360 370 380 390 400
DIEKAFQKAS LDLFNKKTKP SLYLSLHNGN MYTSSLYGCL ASLLSQCSAQ
410 420 430 440 450
DLAGSRIGAF SYGSGLAASF YSLRVSQDAS PGSPLEKLVS SVSDLPERLA
460 470 480 490 500
SRKRVSPEEF TEIMNQREQY YHKVNFTPPG DPNSLFPGTW YLERVDELYR

RKYARHLV
Length:508
Mass (Da):56,934
Last modified:July 1, 1997 - v1
Checksum:i5479DE6F70B3C0F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90884 mRNA. Translation: AAC48727.1.
CR956647 Genomic DNA. Translation: CAN13229.1.
RefSeqiNP_999545.1. NM_214380.2.
UniGeneiSsc.14479.

Genome annotation databases

EnsembliENSSSCT00000007357; ENSSSCP00000007162; ENSSSCG00000006716.
GeneIDi397673.
KEGGissc:397673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90884 mRNA. Translation: AAC48727.1 .
CR956647 Genomic DNA. Translation: CAN13229.1 .
RefSeqi NP_999545.1. NM_214380.2.
UniGenei Ssc.14479.

3D structure databases

ProteinModelPortali O02734.
SMRi O02734. Positions 53-505.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000007357 ; ENSSSCP00000007162 ; ENSSSCG00000006716 .
GeneIDi 397673.
KEGGi ssc:397673.

Organism-specific databases

CTDi 3158.

Phylogenomic databases

eggNOGi COG3425.
GeneTreei ENSGT00390000006096.
HOGENOMi HOG000012351.
HOVERGENi HBG051912.
InParanoidi O02734.
KOi K01641.
OMAi MLVGPEA.
OrthoDBi EOG741Z1W.
TreeFami TF105361.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
BRENDAi 2.3.3.10. 6170.
Reactomei REACT_226431. PPARA activates gene expression.
REACT_253457. Synthesis of Ketone Bodies.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene expression of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase in a poorly ketogenic mammal: effect of starvation during the neonatal period of the piglet."
    Adams S.H., Alho C.S., Asins G., Hegardt F.G., Marrero P.F.
    Biochem. J. 324:65-73(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. Porcine genome sequencing project
    Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiHMCS2_PIG
AccessioniPrimary (citable) accession number: O02734
Secondary accession number(s): A5GFY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3