O02734 (HMCS2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylglutaryl-CoA synthase, mitochondrial
Short name=HMG-CoA synthase
EC=2.3.3.10

Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase

Gene names

Name:

HMGCS2

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Cholesterol metabolism Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: Compara
Molecular_function	hydroxymethylglutaryl-CoA synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Mitochondrion Probable
	Chain	38 – 508	471	Hydroxymethylglutaryl-CoA synthase, mitochondrial		PRO_0000013485
Sites
	Active site	166	1	Potential
Amino acid modifications
	Modified residue	310	1	N6-succinyllysine By similarity
	Modified residue	433	1	Phosphoserine By similarity
	Modified residue	437	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02734 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5479DE6F70B3C0F6	FASTA	508	56,934
	10 20 30 40 50 60 MQRLLTPVRQ VLRVKRAMQE ASFMPPLLPP AAHQRFSTVP AVPVAKADTW PKDVGILALE 70 80 90 100 110 120 VYFPAQYVDQ TDLEKFDNVE AGRYTVGLGQ THMGFCSVQE DINSLCLTVV QRLMERTQLP 130 140 150 160 170 180 WDSVGWLEVG TETIIDKSKS VKTVLMELFQ DSGNTDIEGI DTTNACYGGT ASLFNAANWV 190 200 210 220 230 240 ESSAWDGRYA VVVCGDIAVY PRGNSRPTGG AGAVAMLVGP EAPLALERGL RGTHMENAYD 250 260 270 280 290 300 FYKPNATSEY PLVDGKLSIQ CYLRALDRCY TLYRQKIEKQ WKQAGIERHF TLDDLQFMIF 310 320 330 340 350 360 HTPFCKLVQK SLARLMFSDF LLADSDTQSS LYKGLEAFRG QKLEDTYANK DIEKAFQKAS 370 380 390 400 410 420 LDLFNKKTKP SLYLSLHNGN MYTSSLYGCL ASLLSQCSAQ DLAGSRIGAF SYGSGLAASF 430 440 450 460 470 480 YSLRVSQDAS PGSPLEKLVS SVSDLPERLA SRKRVSPEEF TEIMNQREQY YHKVNFTPPG 490 500 DPNSLFPGTW YLERVDELYR RKYARHLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene expression of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase in a poorly ketogenic mammal: effect of starvation during the neonatal period of the piglet." Adams S.H., Alho C.S., Asins G., Hegardt F.G., Marrero P.F. Biochem. J. 324:65-73(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	Porcine genome sequencing project Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U90884 mRNA. Translation: AAC48727.1. CR956647 Genomic DNA. Translation: CAN13229.1.
RefSeq	NP_999545.1. NM_214380.2.
UniGene	Ssc.14479.
3D structure databases
ProteinModelPortal	O02734.
SMR	O02734. Positions 53-505.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000007357; ENSSSCP00000007162; ENSSSCG00000006716.
GeneID	397673.
KEGG	ssc:397673.
Organism-specific databases
CTD	3158.
Phylogenomic databases
eggNOG	COG3425.
GeneTree	ENSGT00390000006096.
HOGENOM	HOG000012351.
HOVERGEN	HBG051912.
KO	K01641.
OMA	LDDLQFM.
OrthoDB	EOG4H72B9.
Enzyme and pathway databases
BRENDA	2.3.3.10. 6170.
UniPathway	UPA00058; UER00102.
Family and domain databases
Gene3D	3.40.47.10. 1 hit.
InterPro	IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMCS2_PIG

Accession

Primary (citable) accession number: O02734
Secondary accession number(s): A5GFY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program