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O02734

- HMCS2_PIG

UniProt

O02734 - HMCS2_PIG

Protein

Hydroxymethylglutaryl-CoA synthase, mitochondrial

Gene

HMGCS2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

    Catalytic activityi

    Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801SubstrateBy similarity
    Active sitei132 – 1321Proton donor/acceptorPROSITE-ProRule annotation
    Active sitei166 – 1661Acyl-thioester intermediatePROSITE-ProRule annotation
    Binding sitei204 – 2041SubstrateBy similarity
    Binding sitei258 – 2581SubstrateBy similarity
    Active sitei301 – 3011Proton donor/acceptorPROSITE-ProRule annotation
    Binding sitei380 – 3801SubstrateBy similarity

    GO - Molecular functioni

    1. hydroxymethylglutaryl-CoA synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cholesterol biosynthetic process Source: UniProtKB-KW
    2. isoprenoid biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Enzyme and pathway databases

    BRENDAi2.3.3.10. 6170.
    ReactomeiREACT_226431. PPARA activates gene expression.
    UniPathwayiUPA00058; UER00102.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylglutaryl-CoA synthase, mitochondrial (EC:2.3.3.10)
    Short name:
    HMG-CoA synthase
    Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase
    Gene namesi
    Name:HMGCS2
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 4

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Ensembl

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3737MitochondrionCuratedAdd
    BLAST
    Chaini38 – 508471Hydroxymethylglutaryl-CoA synthase, mitochondrialPRO_0000013485Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei52 – 521N6-succinyllysineBy similarity
    Modified residuei243 – 2431N6-acetyllysineBy similarity
    Modified residuei256 – 2561N6-acetyllysine; alternateBy similarity
    Modified residuei256 – 2561N6-succinyllysine; alternateBy similarity
    Modified residuei306 – 3061N6-acetyllysineBy similarity
    Modified residuei310 – 3101N6-acetyllysine; alternateBy similarity
    Modified residuei310 – 3101N6-succinyllysine; alternateBy similarity
    Modified residuei333 – 3331N6-succinyllysineBy similarity
    Modified residuei342 – 3421N6-acetyllysine; alternateBy similarity
    Modified residuei342 – 3421N6-succinyllysine; alternateBy similarity
    Modified residuei350 – 3501N6-acetyllysine; alternateBy similarity
    Modified residuei350 – 3501N6-succinyllysine; alternateBy similarity
    Modified residuei354 – 3541N6-acetyllysine; alternateBy similarity
    Modified residuei354 – 3541N6-succinyllysine; alternateBy similarity
    Modified residuei358 – 3581N6-acetyllysine; alternateBy similarity
    Modified residuei358 – 3581N6-succinyllysine; alternateBy similarity
    Modified residuei437 – 4371N6-acetyllysineBy similarity
    Modified residuei473 – 4731N6-acetyllysine; alternateBy similarity
    Modified residuei473 – 4731N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO02734.
    SMRiO02734. Positions 53-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMG-CoA synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3425.
    GeneTreeiENSGT00390000006096.
    HOGENOMiHOG000012351.
    HOVERGENiHBG051912.
    KOiK01641.
    OMAiMLVGPEA.
    OrthoDBiEOG741Z1W.
    TreeFamiTF105361.

    Family and domain databases

    Gene3Di3.40.47.10. 1 hit.
    InterProiIPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 3 hits.
    TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O02734-1 [UniParc]FASTAAdd to Basket

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    MQRLLTPVRQ VLRVKRAMQE ASFMPPLLPP AAHQRFSTVP AVPVAKADTW    50
    PKDVGILALE VYFPAQYVDQ TDLEKFDNVE AGRYTVGLGQ THMGFCSVQE 100
    DINSLCLTVV QRLMERTQLP WDSVGWLEVG TETIIDKSKS VKTVLMELFQ 150
    DSGNTDIEGI DTTNACYGGT ASLFNAANWV ESSAWDGRYA VVVCGDIAVY 200
    PRGNSRPTGG AGAVAMLVGP EAPLALERGL RGTHMENAYD FYKPNATSEY 250
    PLVDGKLSIQ CYLRALDRCY TLYRQKIEKQ WKQAGIERHF TLDDLQFMIF 300
    HTPFCKLVQK SLARLMFSDF LLADSDTQSS LYKGLEAFRG QKLEDTYANK 350
    DIEKAFQKAS LDLFNKKTKP SLYLSLHNGN MYTSSLYGCL ASLLSQCSAQ 400
    DLAGSRIGAF SYGSGLAASF YSLRVSQDAS PGSPLEKLVS SVSDLPERLA 450
    SRKRVSPEEF TEIMNQREQY YHKVNFTPPG DPNSLFPGTW YLERVDELYR 500
    RKYARHLV 508
    Length:508
    Mass (Da):56,934
    Last modified:July 1, 1997 - v1
    Checksum:i5479DE6F70B3C0F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90884 mRNA. Translation: AAC48727.1.
    CR956647 Genomic DNA. Translation: CAN13229.1.
    RefSeqiNP_999545.1. NM_214380.2.
    UniGeneiSsc.14479.

    Genome annotation databases

    EnsembliENSSSCT00000007357; ENSSSCP00000007162; ENSSSCG00000006716.
    GeneIDi397673.
    KEGGissc:397673.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90884 mRNA. Translation: AAC48727.1 .
    CR956647 Genomic DNA. Translation: CAN13229.1 .
    RefSeqi NP_999545.1. NM_214380.2.
    UniGenei Ssc.14479.

    3D structure databases

    ProteinModelPortali O02734.
    SMRi O02734. Positions 53-505.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000007357 ; ENSSSCP00000007162 ; ENSSSCG00000006716 .
    GeneIDi 397673.
    KEGGi ssc:397673.

    Organism-specific databases

    CTDi 3158.

    Phylogenomic databases

    eggNOGi COG3425.
    GeneTreei ENSGT00390000006096.
    HOGENOMi HOG000012351.
    HOVERGENi HBG051912.
    KOi K01641.
    OMAi MLVGPEA.
    OrthoDBi EOG741Z1W.
    TreeFami TF105361.

    Enzyme and pathway databases

    UniPathwayi UPA00058 ; UER00102 .
    BRENDAi 2.3.3.10. 6170.
    Reactomei REACT_226431. PPARA activates gene expression.

    Family and domain databases

    Gene3Di 3.40.47.10. 1 hit.
    InterProi IPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 3 hits.
    TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene expression of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase in a poorly ketogenic mammal: effect of starvation during the neonatal period of the piglet."
      Adams S.H., Alho C.S., Asins G., Hegardt F.G., Marrero P.F.
      Biochem. J. 324:65-73(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. Porcine genome sequencing project
      Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiHMCS2_PIG
    AccessioniPrimary (citable) accession number: O02734
    Secondary accession number(s): A5GFY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3