O02691 (HCD2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxyacyl-CoA dehydrogenase type-2
EC=1.1.1.35

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 10
3-hydroxy-2-methylbutyryl-CoA dehydrogenase
EC=1.1.1.178
3-hydroxyacyl-CoA dehydrogenase type II
Mitochondrial ribonuclease P protein 2
Short name=Mitochondrial RNase P protein 2
Type II HADH

Gene names

Name:	HSD17B10
Synonyms:	HADH2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends By similarity.
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH.
Subunit structure	Homotetramer. Interacts with MRPP1/TRMT10C and MRPP3/KIAA0391 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: Compara mitochondrial inner membrane Inferred from electronic annotation. Source: Compara mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC 3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 261	260	3-hydroxyacyl-CoA dehydrogenase type-2		PRO_0000054809
Regions
	Nucleotide binding	12 – 37	26	NAD By similarity
Sites
	Active site	168	1	Proton acceptor By similarity
	Binding site	155	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02691 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8C7572B6A9A49780	FASTA	261	27,140
	10 20 30 40 50 60 MAAACRSVKG LVALITGGAS GLGLATAERL VGQGATAVLL DLPNSDGETQ AKKLGKSCAF 70 80 90 100 110 120 APADVTSEKD VQAALTLARE KFGRVDVAVN CAGIAVASKT YNLKKSQAHT LEDFQRVINV 130 140 150 160 170 180 NLIGTFNVIR LVAGEMGQNE PDQGGQRGVI INTASVAAFE GQVGQAAYSA SKGGIVGMTL 190 200 210 220 230 240 PIARDLAPMG IRVMTIAPGL FGTPLLTTLP DKVRNFLASQ VPFPSRLGDP AEYAHLVQAI 250 260 IENSFLNGEV IRLDGAIRMQ P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of cDNA for a newly identified isozyme of bovine liver 3-hydroxyacyl-CoA dehydrogenase and its import into mitochondria." Furuta S., Kobayashi A., Miyazawa S., Hashimoto T. Biochim. Biophys. Acta 1350:317-324(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB002156 mRNA. Translation: BAA19510.1. BC110264 mRNA. Translation: AAI10265.1.
IPI	IPI00699128.
RefSeq	NP_776759.1. NM_174334.3.
UniGene	Bt.5231.
3D structure databases
ProteinModelPortal	O02691.
SMR	O02691. Positions 7-261.
ModBase	Search...
Protein-protein interaction databases
IntAct	O02691. 1 interaction.
STRING	9913.ENSBTAP00000023642.
Proteomic databases
PaxDb	O02691.
PRIDE	O02691.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000023642; ENSBTAP00000023642; ENSBTAG00000017779.
GeneID	281809.
KEGG	bta:281809.
Organism-specific databases
CTD	3028.
Phylogenomic databases
eggNOG	COG1028.
GeneTree	ENSGT00700000104319.
HOVERGEN	HBG002145.
InParanoid	O02691.
KO	K08683.
OMA	HIFENDM.
OrthoDB	EOG46MBKC.
Enzyme and pathway databases
BioCyc	CATTLE:281809-MONOMER.
BRENDA	1.1.1.135. 908.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805721.

Entry information

Entry name

HCD2_BOVIN

Accession

Primary (citable) accession number: O02691
Secondary accession number(s): Q2TBG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program