Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

G protein-activated inward rectifier potassium channel 4

Gene

KCNJ5

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1791Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-SSC-1296041. Activation of G protein gated Potassium channels.
R-SSC-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 4
Short name:
GIRK-4
Alternative name(s):
Cardiac inward rectifier
Short name:
CIR
Heart KATP channel
Inward rectifier K(+) channel Kir3.4
KATP-1
Potassium channel, inwardly rectifying subfamily J member 5
Gene namesi
Name:KCNJ5
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 9

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8686CytoplasmicBy similarityAdd
BLAST
Transmembranei87 – 11125Helical; Name=M1By similarityAdd
BLAST
Topological domaini112 – 13524ExtracellularBy similarityAdd
BLAST
Intramembranei136 – 14712Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei148 – 1547Pore-formingBy similarity
Topological domaini155 – 1639ExtracellularBy similarity
Transmembranei164 – 18522Helical; Name=M2By similarityAdd
BLAST
Topological domaini186 – 419234CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419G protein-activated inward rectifier potassium channel 4PRO_0000154936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO02670.

Expressioni

Gene expression databases

GenevisibleiO02670. SS.

Interactioni

Subunit structurei

May associate with GIRK1 and GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000020421.

Structurei

3D structure databases

ProteinModelPortaliO02670.
SMRiO02670. Positions 197-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi149 – 1546Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOVERGENiHBG006178.
InParanoidiO02670.
KOiK04999.
OMAiMNQDMEI.
OrthoDBiEOG7XPZ5K.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003277. K_chnl_inward-rec_Kir3.4.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01330. KIR34CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

O02670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDSRNAMN QDMEIGVTPR DPKKIPKQAR DYIPIATDRT RLLAEGKKPR
60 70 80 90 100
QRYMEKSGKC NVHHGNVQET YRYLSDLFTT LVDLKWRFNL LVFTMVYTVT
110 120 130 140 150
WLFFGFIWWL IAYIRGDLDH VGDQEWIPCV ENLSGFVSAF LFSIETETTI
160 170 180 190 200
GYGFRVITEK CPEGIVLLLV QAILGSIVNA FMVGCMFVKI SQPKKRAETL
210 220 230 240 250
MFSNHAVISL RDEKLCLMFR VGDLRNSHIV EASIRAKLIK SRQTKEGEFI
260 270 280 290 300
PLNQTDINVG FDTGDDRLFL VSPLIISHEI NEKSPFWEMS RAQLNQEEFE
310 320 330 340 350
VVVILEGMVE ATGMTCQARS SYMDTEVLWG HRFTPVLTLE KGFYEVDYNT
360 370 380 390 400
FHDTYETNTP SCCAKELAEM KREGRLLQYL PSPPLPGGCA GAELEAEAEQ
410
EGEDEPEGLR GSRETRDSV
Length:419
Mass (Da):47,778
Last modified:July 1, 1997 - v1
Checksum:i8FCB123056EB10AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50133 mRNA. Translation: BAA08813.1.
RefSeqiNP_999390.1. NM_214225.1.
UniGeneiSsc.16170.

Genome annotation databases

EnsembliENSSSCT00000027719; ENSSSCP00000020421; ENSSSCG00000021573.
GeneIDi397448.
KEGGissc:397448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50133 mRNA. Translation: BAA08813.1.
RefSeqiNP_999390.1. NM_214225.1.
UniGeneiSsc.16170.

3D structure databases

ProteinModelPortaliO02670.
SMRiO02670. Positions 197-370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000020421.

Proteomic databases

PaxDbiO02670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000027719; ENSSSCP00000020421; ENSSSCG00000021573.
GeneIDi397448.
KEGGissc:397448.

Organism-specific databases

CTDi3762.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOVERGENiHBG006178.
InParanoidiO02670.
KOiK04999.
OMAiMNQDMEI.
OrthoDBiEOG7XPZ5K.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiR-SSC-1296041. Activation of G protein gated Potassium channels.
R-SSC-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Gene expression databases

GenevisibleiO02670. SS.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003277. K_chnl_inward-rec_Kir3.4.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01330. KIR34CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Functional characterization and localization of a cardiac-type inwardly rectifying K+ channel."
    Iizuka M., Kubo Y., Tsunenari I., Pan C.X., Akiba I., Kono T.
    Recept. Channels 3:299-315(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiKCNJ5_PIG
AccessioniPrimary (citable) accession number: O02670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.