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Protein

Mannose-binding protein C

Gene

MBL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Complement activation lectin pathway, Complement pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Lectin, Mannose-binding

Enzyme and pathway databases

ReactomeiR-BTA-166662. Lectin pathway of complement activation.
R-BTA-166663. Initial triggering of complement.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-binding protein C
Short name:
MBP-C
Alternative name(s):
Mannan-binding protein
Gene namesi
Name:MBL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 249229Mannose-binding protein CPRO_0000017397Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 – 34InterchainPROSITE-ProRule annotation
Disulfide bondi39 – 39InterchainPROSITE-ProRule annotation
Modified residuei48 – 4814-hydroxyprolineSequence analysis
Modified residuei63 – 6314-hydroxyprolineSequence analysis
Modified residuei74 – 7414-hydroxyprolineSequence analysis
Modified residuei83 – 8314-hydroxyprolineSequence analysis
Modified residuei86 – 8614-hydroxyprolineSequence analysis
Disulfide bondi156 ↔ 245PROSITE-ProRule annotation
Disulfide bondi223 ↔ 237PROSITE-ProRule annotation

Post-translational modificationi

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.By similarity

Keywords - PTMi

Disulfide bond, Hydroxylation

Proteomic databases

PaxDbiO02659.
PRIDEiO02659.

Interactioni

Subunit structurei

Oligomeric complex of 3 or more homotrimers. Interacts with MASP1 and MASP2 (By similarity). Interacts with MEP1A and MEP1B and may inhibit their catalytic activity (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009270.

Structurei

3D structure databases

ProteinModelPortaliO02659.
SMRiO02659. Positions 109-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 10159Collagen-likeAdd
BLAST
Domaini135 – 246112C-type lectinPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili113 – 13119By similarityAdd
BLAST

Domaini

The coiled-coil domain mediates trimerization.By similarity

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 collagen-like domain.Curated

Keywords - Domaini

Coiled coil, Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IS48. Eukaryota.
ENOG4110NRD. LUCA.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiO02659.
KOiK03991.
OMAiQKTCHVV.
OrthoDBiEOG7VTDPW.
TreeFamiTF330481.

Family and domain databases

Gene3Di3.10.100.10. 2 hits.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O02659-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFTSLPFL LLTAVTASCA DTETENCENI RKTCPVIACG PPGINGIPGK
60 70 80 90 100
DGRDGAKGEK GEPGQGLRGS QGPPGKMGPQ GTPGIPGIPG PIGQKGDPGE
110 120 130 140 150
NMGDYIRLAT SERATLQSEL NQIKNWLIFS LGKRVGKKAF FTNGKKMPFN
160 170 180 190 200
EVKTLCAQFQ GRVATPMNAE ENRALKDLVT EEAFLGITDQ ETEGKFVDLT
210 220 230 240
GKGVTYQNWN DGEPNNASPG EHCVTLLSDG TWNDIACSAS FLTVCEFSL
Length:249
Mass (Da):26,471
Last modified:July 1, 1997 - v1
Checksum:i29FC9F5927A66DD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D73408 mRNA. Translation: BAA18935.1.
BC134704 mRNA. Translation: AAI34705.1.
RefSeqiNP_776532.1. NM_174107.2.
XP_005225409.1. XM_005225352.3.
UniGeneiBt.360.

Genome annotation databases

EnsembliENSBTAT00000009270; ENSBTAP00000009270; ENSBTAG00000007049.
GeneIDi281297.
KEGGibta:281297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D73408 mRNA. Translation: BAA18935.1.
BC134704 mRNA. Translation: AAI34705.1.
RefSeqiNP_776532.1. NM_174107.2.
XP_005225409.1. XM_005225352.3.
UniGeneiBt.360.

3D structure databases

ProteinModelPortaliO02659.
SMRiO02659. Positions 109-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009270.

Proteomic databases

PaxDbiO02659.
PRIDEiO02659.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000009270; ENSBTAP00000009270; ENSBTAG00000007049.
GeneIDi281297.
KEGGibta:281297.

Organism-specific databases

CTDi4153.

Phylogenomic databases

eggNOGiENOG410IS48. Eukaryota.
ENOG4110NRD. LUCA.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiO02659.
KOiK03991.
OMAiQKTCHVV.
OrthoDBiEOG7VTDPW.
TreeFamiTF330481.

Enzyme and pathway databases

ReactomeiR-BTA-166662. Lectin pathway of complement activation.
R-BTA-166663. Initial triggering of complement.

Family and domain databases

Gene3Di3.10.100.10. 2 hits.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a cDNA encoding bovine mannan-binding protein."
    Kawai T., Suzuki Y., Eda S., Ohtani K., Kase T., Fujinaga Y., Sakamoto T., Kurimura T., Wakamiya N.
    Gene 186:161-165(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Testis.

Entry informationi

Entry nameiMBL2_BOVIN
AccessioniPrimary (citable) accession number: O02659
Secondary accession number(s): A4IFQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.