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Protein

Serine/threonine-protein phosphatase PP1-gamma

Gene

gsp-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Probable phosphatase which plays a redundant role with gsp-4 in spermatogenesis by regulating sister chromatid segregation during meiosis (PubMed:16943775, PubMed:22042574). In addition, involved in sperm motility by controlling the dynamic disassembly of major sperm proteins (MSP) in the spermatozoan pseudopodium (PubMed:22042574).2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Manganese 1By similarity1
Metal bindingi64Manganese 1By similarity1
Metal bindingi90Manganese 1By similarity1
Metal bindingi90Manganese 2By similarity1
Metal bindingi122Manganese 2By similarity1
Active sitei123Proton donorBy similarity1
Metal bindingi172Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • flagellated sperm motility Source: WormBase
  • male meiosis chromosome segregation Source: WormBase
  • oviposition Source: WormBase
  • regulation of pseudopodium assembly Source: WormBase
  • reproduction Source: WormBase
  • spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-gammaCurated (EC:3.1.3.16UniRule annotation)
Alternative name(s):
CeGLC-7-gammaCurated
Glc seven-like phosphatase 3Imported
Gene namesi
Name:gsp-3Imported
ORF Names:W09C3.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiW09C3.6; CE14754; WBGene00021113; gsp-3.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: WormBase
  • cytoplasm Source: WormBase
  • nuclear chromatin Source: WormBase
  • pseudopodium Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Chromosome, Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype (PubMed:22042574). Simultaneous RNAi-mediated knockdown of gsp-3 and gsp-4 results in male sterility due to chromosome segregation defects during sperm meiosis (PubMed:16943775). gsp-3 and gsp-4 double mutant hermaphrodites have egg laying defects and no viable progeny. gsp-3, gsp-4 and him-8 triple mutants have incomplete separation of sister chromatids during the second meiotic segregation. Mislocalization of MSP in activated sperm. In addition, have partial reduction in mpk-1 phosphorylation and air-2 association with bivalent chromosomes in the most proximal oocyte in response to MSP (PubMed:22042574).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004363881 – 305Serine/threonine-protein phosphatase PP1-gammaCuratedAdd BLAST305

Proteomic databases

EPDiO02658.
PaxDbiO02658.
PeptideAtlasiO02658.

Expressioni

Tissue specificityi

Expressed in male germline including spermatocytes, spermatids and spermatozoa.2 Publications

Developmental stagei

Expressed at all stages of spermatocyte meiosis.2 Publications

Gene expression databases

BgeeiWBGene00021113.

Interactioni

Protein-protein interaction databases

DIPiDIP-27281N.
MINTiMINT-1115524.
STRINGi6239.W09C3.6.

Structurei

3D structure databases

ProteinModelPortaliO02658.
SMRiO02658.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiO02658.
KOiK01090.
OMAiCCTVAKS.
OrthoDBiEOG091G0COK.
PhylomeDBiO02658.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O02658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPMDVDNL MSRLLNVGMS GGRLTTSVNE QELQTCCAVA KSVFASQASL
60 70 80 90 100
LEVEPPIIVC GDIHGQYSDL LRIFDKNGFP PDVNFLFLGD YVDRGRQNIE
110 120 130 140 150
TICLMLCFKI KYPENFFMLR GNHECPAINR VYGFYEECNR RYKSTRLWSI
160 170 180 190 200
FQDTFNWMPL CGLIGSRILC MHGGLSPHLQ TLDQLRQLPR PQDPPNPSIG
210 220 230 240 250
IDLLWADPDQ WVKGWQANTR GVSYVFGQDV VADVCSRLDI DLVARAHQVV
260 270 280 290 300
QDGYEFFASK KMVTIFSAPH YCGQFDNSAA TMKVDENMVC TFVMYKPTPK

SMRRG
Length:305
Mass (Da):34,596
Last modified:July 1, 1997 - v1
Checksum:i1D503E11DE0A5B6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD64235.1.
PIRiT34462.
RefSeqiNP_491429.1. NM_059028.3.

Genome annotation databases

EnsemblMetazoaiW09C3.6; W09C3.6; WBGene00021113.
GeneIDi172082.
KEGGicel:CELE_W09C3.6.
UCSCiW09C3.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD64235.1.
PIRiT34462.
RefSeqiNP_491429.1. NM_059028.3.

3D structure databases

ProteinModelPortaliO02658.
SMRiO02658.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-27281N.
MINTiMINT-1115524.
STRINGi6239.W09C3.6.

Proteomic databases

EPDiO02658.
PaxDbiO02658.
PeptideAtlasiO02658.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiW09C3.6; W09C3.6; WBGene00021113.
GeneIDi172082.
KEGGicel:CELE_W09C3.6.
UCSCiW09C3.6. c. elegans.

Organism-specific databases

CTDi172082.
WormBaseiW09C3.6; CE14754; WBGene00021113; gsp-3.

Phylogenomic databases

eggNOGiKOG0374. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
InParanoidiO02658.
KOiK01090.
OMAiCCTVAKS.
OrthoDBiEOG091G0COK.
PhylomeDBiO02658.

Miscellaneous databases

PROiO02658.

Gene expression databases

BgeeiWBGene00021113.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLC7C_CAEEL
AccessioniPrimary (citable) accession number: O02658
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 8, 2016
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Caution

Due to the high similarity between gsp-3 and gsp-4, the antibody used to study expression does not distinguish between the two proteins.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.