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Protein

Serine/threonine-protein phosphatase PP1-gamma

Gene

gsp-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable phosphatase which plays a redundant role with gsp-4 in spermatogenesis by regulating sister chromatid segregation during meiosis (PubMed:16943775, PubMed:22042574). In addition, involved in sperm motility by controlling the dynamic disassembly of major sperm proteins (MSP) in the spermatozoan pseudopodium (PubMed:22042574).2 Publications

Caution

Due to the high similarity between gsp-3 and gsp-4, the antibody used to study expression does not distinguish between the two proteins.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Manganese 1By similarity1
Metal bindingi64Manganese 1By similarity1
Metal bindingi90Manganese 1By similarity1
Metal bindingi90Manganese 2By similarity1
Metal bindingi122Manganese 2By similarity1
Active sitei123Proton donorBy similarity1
Metal bindingi172Manganese 2By similarity1
Metal bindingi247Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • flagellated sperm motility Source: WormBase
  • male meiosis chromosome segregation Source: WormBase
  • oviposition Source: WormBase
  • regulation of pseudopodium assembly Source: WormBase
  • reproduction Source: WormBase
  • spermatogenesis Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processDifferentiation, Spermatogenesis
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-gammaCurated (EC:3.1.3.16UniRule annotation)
Alternative name(s):
CeGLC-7-gammaCurated
Glc seven-like phosphatase 3Imported
Gene namesi
Name:gsp-3Imported
ORF Names:W09C3.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiW09C3.6 ; CE14754 ; WBGene00021113 ; gsp-3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Chromosome, Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype (PubMed:22042574). Simultaneous RNAi-mediated knockdown of gsp-3 and gsp-4 results in male sterility due to chromosome segregation defects during sperm meiosis (PubMed:16943775). gsp-3 and gsp-4 double mutant hermaphrodites have egg laying defects and no viable progeny. gsp-3, gsp-4 and him-8 triple mutants have incomplete separation of sister chromatids during the second meiotic segregation. Mislocalization of MSP in activated sperm. In addition, have partial reduction in mpk-1 phosphorylation and air-2 association with bivalent chromosomes in the most proximal oocyte in response to MSP (PubMed:22042574).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004363881 – 305Serine/threonine-protein phosphatase PP1-gammaCuratedAdd BLAST305

Proteomic databases

EPDiO02658
PaxDbiO02658
PeptideAtlasiO02658

Expressioni

Tissue specificityi

Expressed in male germline including spermatocytes, spermatids and spermatozoa.2 Publications

Developmental stagei

Expressed at all stages of spermatocyte meiosis.2 Publications

Gene expression databases

BgeeiWBGene00021113

Interactioni

Protein-protein interaction databases

DIPiDIP-27281N
IntActiO02658, 1 interactor
STRINGi6239.W09C3.6

Structurei

3D structure databases

ProteinModelPortaliO02658
SMRiO02658
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0374 Eukaryota
COG0639 LUCA
GeneTreeiENSGT00530000062911
HOGENOMiHOG000172697
InParanoidiO02658
KOiK01090
OMAiCCTVAKS
OrthoDBiEOG091G0COK
PhylomeDBiO02658

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
IPR031675 STPPase_N
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF16891 STPPase_N, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

O02658-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPMDVDNL MSRLLNVGMS GGRLTTSVNE QELQTCCAVA KSVFASQASL
60 70 80 90 100
LEVEPPIIVC GDIHGQYSDL LRIFDKNGFP PDVNFLFLGD YVDRGRQNIE
110 120 130 140 150
TICLMLCFKI KYPENFFMLR GNHECPAINR VYGFYEECNR RYKSTRLWSI
160 170 180 190 200
FQDTFNWMPL CGLIGSRILC MHGGLSPHLQ TLDQLRQLPR PQDPPNPSIG
210 220 230 240 250
IDLLWADPDQ WVKGWQANTR GVSYVFGQDV VADVCSRLDI DLVARAHQVV
260 270 280 290 300
QDGYEFFASK KMVTIFSAPH YCGQFDNSAA TMKVDENMVC TFVMYKPTPK

SMRRG
Length:305
Mass (Da):34,596
Last modified:July 1, 1997 - v1
Checksum:i1D503E11DE0A5B6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA Translation: CCD64235.1
PIRiT34462
RefSeqiNP_491429.1, NM_059028.3

Genome annotation databases

EnsemblMetazoaiW09C3.6; W09C3.6; WBGene00021113
GeneIDi172082
KEGGicel:CELE_W09C3.6
UCSCiW09C3.6 c. elegans

Similar proteinsi

Entry informationi

Entry nameiGLC7C_CAEEL
AccessioniPrimary (citable) accession number: O02658
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 8, 2016
Last sequence update: July 1, 1997
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health