Reviewed, UniProtKB/Swiss-Prot O02640 (MDHM_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable malate dehydrogenase, mitochondrial
    EC=1.1.1.37

Gene names

Name:	mdh-1
ORF Names:	F20H11.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix Potential.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 341		Probable malate dehydrogenase, mitochondrial		PRO_0000018632
Regions
	Nucleotide binding	35 – 41	7	NAD By similarity
	Nucleotide binding	144 – 146	3	NAD By similarity
Sites
	Active site	204	1	Proton acceptor By similarity
	Binding site	61	1	NAD By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	121	1	NAD By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	255	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02640-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7515F9C1727AEFB5	FASTA	341	35,120
	10 20 30 40 50 60 MSLPAKTLVQ AAANSGLRAV SVRHSSQAPK VALLGAAGGI GQPLGLLLKQ DPLVAHLALY 70 80 90 100 110 120 DVVNTPGVAA DLSHIDSNAK VTAHTGPKEL YAAVENADVI VIPAGVPRKP GMTRDDLFNT 130 140 150 160 170 180 NAGIVRDLAA VIAKASPKAL IAIITNPVNS TVPIASEVLK KAGVYDPKRV FGVTTLDVVR 190 200 210 220 230 240 SQAFVSELKG HDASKTVVPV VGGHAGITII PLLSQVKPST KFSEEEISKL TPRIQDAGTE 250 260 270 280 290 300 VVNAKAGAGS ATLSMALAGA RFANALVRGI KGEKNVQCAY VASDAVKGVE YFSTPVELGP 310 320 330 340 NGVEKILGVG KVSAYEQKLI DASVPELNKN IAKGVAFVKG N

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF002197 Genomic DNA. Translation: AAB53985.1.
PIR	C88486.
RefSeq	NP_498457.1.
UniGene	Cel.5076
3D structure databases
HSSP	HSSP built from PDB template 1MLD based on UniProtKB P00346.
ModBase	Search...
Genome annotation databases
Ensembl	F20H11.3. Caenorhabditis elegans. [Contig view]
GeneID	175936.
KEGG	cel:F20H11.3.
Organism-specific databases
WormBase	WBGene00003162. mdh-1.
WormPep	F20H11.3. CE09512. [WorfDB]
Phylogenomic databases
OMA	O02640. TNSYEDI.
Enzyme and pathway databases
BRENDA	1.1.1.37. 672.
Gene expression databases
ArrayExpress	O02640.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	890380.

Entry information

Entry name

MDHM_CAEEL

Accession

Primary (citable) accession number: O02640

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project