O02640 (MDHM_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable malate dehydrogenase, mitochondrial
EC=1.1.1.37

Gene names

Name:	mdh-1
ORF Names:	F20H11.3

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix Potential.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from sequence or structural similarity PubMed 12875742. Source: WormBase tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 21611156. Source: WormBase
Molecular_function	L-malate dehydrogenase activity Inferred from sequence or structural similarity PubMed 12875742. Source: WormBase nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 341		Probable malate dehydrogenase, mitochondrial		PRO_0000018632
Regions
	Nucleotide binding	35 – 41	7	NAD By similarity
	Nucleotide binding	144 – 146	3	NAD By similarity
Sites
	Active site	204	1	Proton acceptor By similarity
	Binding site	61	1	NAD By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	121	1	NAD By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	255	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O02640 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7515F9C1727AEFB5	FASTA	341	35,120
	10 20 30 40 50 60 MSLPAKTLVQ AAANSGLRAV SVRHSSQAPK VALLGAAGGI GQPLGLLLKQ DPLVAHLALY 70 80 90 100 110 120 DVVNTPGVAA DLSHIDSNAK VTAHTGPKEL YAAVENADVI VIPAGVPRKP GMTRDDLFNT 130 140 150 160 170 180 NAGIVRDLAA VIAKASPKAL IAIITNPVNS TVPIASEVLK KAGVYDPKRV FGVTTLDVVR 190 200 210 220 230 240 SQAFVSELKG HDASKTVVPV VGGHAGITII PLLSQVKPST KFSEEEISKL TPRIQDAGTE 250 260 270 280 290 300 VVNAKAGAGS ATLSMALAGA RFANALVRGI KGEKNVQCAY VASDAVKGVE YFSTPVELGP 310 320 330 340 NGVEKILGVG KVSAYEQKLI DASVPELNKN IAKGVAFVKG N

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081189 Genomic DNA. Translation: CCD69776.1.
PIR	C88486.
RefSeq	NP_498457.1. NM_066056.5.
3D structure databases
ProteinModelPortal	O02640.
SMR	O02640. Positions 30-339.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.F20H11.3.2.
Proteomic databases
PaxDb	O02640.
PRIDE	O02640.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F20H11.3.1; F20H11.3.1; F20H11.3. F20H11.3.2; F20H11.3.2; F20H11.3.
GeneID	175936.
KEGG	cel:CELE_F20H11.3.
UCSC	F20H11.3.1. c. elegans.
Organism-specific databases
CTD	175936.
WormBase	F20H11.3; CE09512; WBGene00003162; mdh-1.
Phylogenomic databases
eggNOG	COG0039.
GeneTree	ENSGT00390000016686.
HOGENOM	HOG000213792.
InParanoid	O02640.
KO	K00026.
OMA	VEVKGFA.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit. PTHR11540:SF1. PTHR11540:SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	890380.

Entry information

Entry name

MDHM_CAEEL

Accession

Primary (citable) accession number: O02640

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project