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Protein

MAP kinase-activating death domain protein

Gene

aex-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a novel and significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Plays an important regulatory role in physiological cell death, caspase-mediated apoptosis (By similarity). Converts GDP-bound inactive form of rab-3 and cab-1 to the GTP-bound active forms. Regulator of presynaptic activity that interacts with rab-3 to regulate synaptic vesicle release (PubMed:9136770, PubMed:10970871). Is also a regulator of the cab-1 synaptic transmission pathway (PubMed:10970871). Probably by converting rab-3 to its GTP-bound active form, plays a role in the recruitment of endophilin unc-57 to synaptic vesicles (PubMed:21029864). Probably by activating rab-3 and thus regulating the trafficking of dense-core vesicles, plays a role in AVG neuron-mediated formation of the right axon tract of the ventral nerve cord (PubMed:27116976). Regulates anterior body muscle contractions (aBOC) and the expulsion steps during the defecation motor program (DMP) (PubMed:18852466). Probably by regulating DMP, required for fatty acid uptake by intestinal cells (PubMed:25849533).By similarity6 Publications

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: WormBase

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • chemical synaptic transmission Source: WormBase
  • defecation Source: WormBase
  • lipid transport involved in lipid storage Source: UniProtKB
  • mating Source: WormBase
  • oviposition Source: WormBase
  • positive regulation of acetylcholine secretion, neurotransmission Source: WormBase
  • positive regulation of anterior/posterior axon guidance Source: UniProtKB
  • positive regulation of defecation Source: UniProtKB
  • positive regulation of intestinal lipid absorption Source: UniProtKB
  • ventral cord development Source: UniProtKB

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-CEL-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-activating death domain protein
Alternative name(s):
Aboc, expulsion defective protein 3
Regulator of presynaptic activity aex-3
Gene namesi
Name:aex-3
ORF Names:C02H7.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC02H7.3a ; CE16806 ; WBGene00000086 ; aex-3
C02H7.3b ; CE30850 ; WBGene00000086 ; aex-3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Worms exhibit pleiotropic behavioral defects that are suggestive of reduced synaptic transmission (PubMed:9136770). RNAi-mediated knockdown in the intestine causes a mild defect in the expulsion step of the defecation cycle (PubMed:18852466).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644691 – 1409MAP kinase-activating death domain proteinAdd BLAST1409

Proteomic databases

EPDiO02626
PaxDbiO02626
PeptideAtlasiO02626
PRIDEiO02626

Expressioni

Tissue specificityi

Expressed in nearly all neurons.1 Publication

Gene expression databases

BgeeiWBGene00000086

Interactioni

Subunit structurei

Interacts with cab-1.1 Publication

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: WormBase

Protein-protein interaction databases

STRINGi6239.C02H7.3a

Structurei

3D structure databases

ProteinModelPortaliO02626
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 230uDENNPROSITE-ProRule annotationAdd BLAST205
Domaini251 – 390cDENNPROSITE-ProRule annotationAdd BLAST140
Domaini392 – 496dDENNPROSITE-ProRule annotationAdd BLAST105
Domaini1109 – 1184DeathAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi604 – 632Ser-richAdd BLAST29
Compositional biasi962 – 1069Pro-richAdd BLAST108

Sequence similaritiesi

Belongs to the MADD family.Curated

Phylogenomic databases

eggNOGiKOG3570 Eukaryota
ENOG410XP23 LUCA
GeneTreeiENSGT00760000118819
HOGENOMiHOG000231438
InParanoidiO02626
OMAiKRLVDCC
OrthoDBiEOG091G00AV
PhylomeDBiO02626

Family and domain databases

InterProiView protein in InterPro
IPR001194 cDENN_dom
IPR005112 dDENN_dom
IPR037516 Tripartite_DENN
IPR005113 uDENN_dom
PfamiView protein in Pfam
PF02141 DENN, 1 hit
PF03456 uDENN, 1 hit
SMARTiView protein in SMART
SM00801 dDENN, 1 hit
SM00799 DENN, 1 hit
SM00800 uDENN, 1 hit
PROSITEiView protein in PROSITE
PS50211 DENN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: O02626-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNDKEKEICP RLIDFLVVVG KRNRTRGASQ SSPDATTDTT VTYPEILRRY
60 70 80 90 100
PTDDHKDFIL PTDVTVFCQP EGCTTTSARL RKNARNDPQF FVFMLTEKDS
110 120 130 140 150
AKVRYGICLN FYQSFDRRST PKDEIKKVPD DAHHKKRDSH VSLTSLCFIS
160 170 180 190 200
HHPFVSIFHQ VLLLLKRIID SSNHRAAQRT GLKDVVWAIL TGHYNEPIVP
210 220 230 240 250
EVMKEIKEIE TWILMLLSSP VPVPGKTKVQ IEVMPMDLSQ VFEFALPDHT
260 270 280 290 300
RFTLIDFPLH IPFEILGIDM ALRVLTAAML EFKIVIQSRN YNAVSMCILS
310 320 330 340 350
IVALLYPLEY MFPVIPLLPA YMPSAEQLLL APTPFLIGVP SSFFHHRKIR
360 370 380 390 400
ELPSDVILVD LDTNCLQVPD DLYIPDLPEP DATHLKERLK NAINKMTTMT
410 420 430 440 450
VDNETSVTDA DFGIDIDSVD VACRVAMVQF FNSANVFGNF SEHTRTLRLY
460 470 480 490 500
PRPVVSLQTD SFLRSRPQCT QLITDLCRTQ AVEYFAECCL CPKNETFVRV
510 520 530 540 550
QAGIESAEQV GDKPKWFSES LMPVHFMVYP NNSTLDSAIR VYNAEIDNDD
560 570 580 590 600
YEDDSATSTE NSNSIDDLVF DENQVTDAGG EVTKPLAEVN YIYKEPMTLE
610 620 630 640 650
LPQSESVVSI DSSLSSGRSS PDSSLSTSAV DSEADFARLA DNLALKSNSQ
660 670 680 690 700
GAFSFDHGSD SEYESTPVSQ RRKTIHNPGS DASDTPTSRG SIKSGLRMKG
710 720 730 740 750
LTTLTDSGEK VLGPSLMNAI NGYAEKSQSV FSQVINKTAP KAQALKERTM
760 770 780 790 800
KPLANRIEQS QHIVRSKTQP NPTSQQTANQ QSKNQQTVKE FCDQALVGQS
810 820 830 840 850
VGMFSAPKLK RLMEDESLRE LVCSKLNLGL EVKLSEDEYV KEVQLTKGQF
860 870 880 890 900
KAYVKILKAC LEGIEVSFNT PGCCGFASVF HVLEIAHTHY WAMGGGEVIT
910 920 930 940 950
PSSSAPSTMT TPSEHSNDIL KESRPKLPAS TIDLRTPTKP LGQNVTPTST
960 970 980 990 1000
NNHEIAQSTR SPALPPPVPP REAPPIPKRN PPPLGAPPKV PEGARAPPPL
1010 1020 1030 1040 1050
PPRPKVKTTA VDETPQNLVP NNQPAQPSSP SFLADADEQT KPLLKPAPPT
1060 1070 1080 1090 1100
TLPVGKQEPC KVLPTPNEPV RHYIYQELIL AVQHQIWQNL QFWENAFVDL
1110 1120 1130 1140 1150
VAQEREIVGM DQEPSEMIDR YSALNDSEKK RLELEEDRLL STLLHNMTAY
1160 1170 1180 1190 1200
MIMCGTGQKA LQQKVRRLLG KAHIGLVCSK EINKLLDELP STQGNFIPLK
1210 1220 1230 1240 1250
PLGSRLVQKQ SFTVCPGQSS DGQMMFMEVC DDAVVLRSIT GAATERWWYE
1260 1270 1280 1290 1300
RLVNITYSPK TKILCLWRRH DDKVHMHKFH TKKCRELYQC MKAAMERAAA
1310 1320 1330 1340 1350
RGKVNVEGRA LGGEFPVHDT ETNQGGLLQV RCDGVAVIFA HNQIFIGLSN
1360 1370 1380 1390 1400
IKKCNTFGGN VFLLEEFDRK KGEIIQRRYF SQMADQICYA VLCVFSLAAA

GHKKEEHSK
Length:1,409
Mass (Da):157,459
Last modified:July 1, 1997 - v1
Checksum:i2DDE6395AC963313
GO
Isoform b (identifier: O02626-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1385-1409: DQICYAVLCVFSLAAAGHKKEEHSK → VDIAWAMHRVFSVQFAISCQKDTN

Note: No experimental confirmation available.
Show »
Length:1,408
Mass (Da):157,478
Checksum:i16BBDA4252C87C29
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0155551385 – 1409DQICY…EEHSK → VDIAWAMHRVFSVQFAISCQ KDTN in isoform b. CuratedAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93842 mRNA Translation: AAB52421.1
FO080298 Genomic DNA Translation: CCD62710.1
FO080298 Genomic DNA Translation: CCD62711.1
PIRiT37188
RefSeqiNP_001024342.1, NM_001029171.3 [O02626-2]
NP_741710.1, NM_171618.3 [O02626-1]
UniGeneiCel.18148

Genome annotation databases

EnsemblMetazoaiC02H7.3a; C02H7.3a; WBGene00000086 [O02626-1]
GeneIDi180420
KEGGicel:CELE_C02H7.3
UCSCiC02H7.3a c. elegans [O02626-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMADD_CAEEL
AccessioniPrimary (citable) accession number: O02626
Secondary accession number(s): Q27467, Q8MQF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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