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Protein

MAP kinase-activating death domain protein

Gene

aex-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a novel and significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Plays an important regulatory role in physiological cell death, caspase-mediated apoptosis (By similarity). Converts GDP-bound inactive form of rab-3 and cab-1 to the GTP-bound active forms. Novel regulator of presynaptic activity that interacts with rab-3 to regulate synaptic vesicle release. Is also a regulator of the cab-1 synaptic transmission pathway.By similarity2 Publications

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: WormBase

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • defecation Source: WormBase
  • mating Source: WormBase
  • oviposition Source: WormBase
  • positive regulation of acetylcholine secretion, neurotransmission Source: WormBase
  • synaptic transmission Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-activating death domain protein
Alternative name(s):
Aboc, expulsion defective protein 3
Regulator of presynaptic activity aex-3
Gene namesi
Name:aex-3
ORF Names:C02H7.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC02H7.3a; CE16806; WBGene00000086; aex-3.
C02H7.3b; CE30850; WBGene00000086; aex-3.

Subcellular locationi

GO - Cellular componenti

  • cell Source: WormBase
  • cytoplasm Source: UniProtKB-SubCell
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Worms exhibit pleiotropic behavioral defects that are suggestive of reduced synaptic transmission.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14091409MAP kinase-activating death domain proteinPRO_0000064469Add
BLAST

Proteomic databases

EPDiO02626.
PaxDbiO02626.
PRIDEiO02626.

Expressioni

Tissue specificityi

Expressed in nearly all neurons.1 Publication

Interactioni

Subunit structurei

Interacts with cab-1.1 Publication

Protein-protein interaction databases

STRINGi6239.C02H7.3a.

Structurei

3D structure databases

ProteinModelPortaliO02626.
SMRiO02626. Positions 263-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 11276UDENNAdd
BLAST
Domaini141 – 364224DENNPROSITE-ProRule annotationAdd
BLAST
Domaini419 – 48971dDENNPROSITE-ProRule annotationAdd
BLAST
Domaini1109 – 118476DeathAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi604 – 63229Ser-richAdd
BLAST
Compositional biasi962 – 1069108Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the MADD family.Curated
Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 death domain.Curated
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3570. Eukaryota.
ENOG410XP23. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000231438.
InParanoidiO02626.
OMAiMHSQVFI.
OrthoDBiEOG708VXV.
PhylomeDBiO02626.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: O02626-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNDKEKEICP RLIDFLVVVG KRNRTRGASQ SSPDATTDTT VTYPEILRRY
60 70 80 90 100
PTDDHKDFIL PTDVTVFCQP EGCTTTSARL RKNARNDPQF FVFMLTEKDS
110 120 130 140 150
AKVRYGICLN FYQSFDRRST PKDEIKKVPD DAHHKKRDSH VSLTSLCFIS
160 170 180 190 200
HHPFVSIFHQ VLLLLKRIID SSNHRAAQRT GLKDVVWAIL TGHYNEPIVP
210 220 230 240 250
EVMKEIKEIE TWILMLLSSP VPVPGKTKVQ IEVMPMDLSQ VFEFALPDHT
260 270 280 290 300
RFTLIDFPLH IPFEILGIDM ALRVLTAAML EFKIVIQSRN YNAVSMCILS
310 320 330 340 350
IVALLYPLEY MFPVIPLLPA YMPSAEQLLL APTPFLIGVP SSFFHHRKIR
360 370 380 390 400
ELPSDVILVD LDTNCLQVPD DLYIPDLPEP DATHLKERLK NAINKMTTMT
410 420 430 440 450
VDNETSVTDA DFGIDIDSVD VACRVAMVQF FNSANVFGNF SEHTRTLRLY
460 470 480 490 500
PRPVVSLQTD SFLRSRPQCT QLITDLCRTQ AVEYFAECCL CPKNETFVRV
510 520 530 540 550
QAGIESAEQV GDKPKWFSES LMPVHFMVYP NNSTLDSAIR VYNAEIDNDD
560 570 580 590 600
YEDDSATSTE NSNSIDDLVF DENQVTDAGG EVTKPLAEVN YIYKEPMTLE
610 620 630 640 650
LPQSESVVSI DSSLSSGRSS PDSSLSTSAV DSEADFARLA DNLALKSNSQ
660 670 680 690 700
GAFSFDHGSD SEYESTPVSQ RRKTIHNPGS DASDTPTSRG SIKSGLRMKG
710 720 730 740 750
LTTLTDSGEK VLGPSLMNAI NGYAEKSQSV FSQVINKTAP KAQALKERTM
760 770 780 790 800
KPLANRIEQS QHIVRSKTQP NPTSQQTANQ QSKNQQTVKE FCDQALVGQS
810 820 830 840 850
VGMFSAPKLK RLMEDESLRE LVCSKLNLGL EVKLSEDEYV KEVQLTKGQF
860 870 880 890 900
KAYVKILKAC LEGIEVSFNT PGCCGFASVF HVLEIAHTHY WAMGGGEVIT
910 920 930 940 950
PSSSAPSTMT TPSEHSNDIL KESRPKLPAS TIDLRTPTKP LGQNVTPTST
960 970 980 990 1000
NNHEIAQSTR SPALPPPVPP REAPPIPKRN PPPLGAPPKV PEGARAPPPL
1010 1020 1030 1040 1050
PPRPKVKTTA VDETPQNLVP NNQPAQPSSP SFLADADEQT KPLLKPAPPT
1060 1070 1080 1090 1100
TLPVGKQEPC KVLPTPNEPV RHYIYQELIL AVQHQIWQNL QFWENAFVDL
1110 1120 1130 1140 1150
VAQEREIVGM DQEPSEMIDR YSALNDSEKK RLELEEDRLL STLLHNMTAY
1160 1170 1180 1190 1200
MIMCGTGQKA LQQKVRRLLG KAHIGLVCSK EINKLLDELP STQGNFIPLK
1210 1220 1230 1240 1250
PLGSRLVQKQ SFTVCPGQSS DGQMMFMEVC DDAVVLRSIT GAATERWWYE
1260 1270 1280 1290 1300
RLVNITYSPK TKILCLWRRH DDKVHMHKFH TKKCRELYQC MKAAMERAAA
1310 1320 1330 1340 1350
RGKVNVEGRA LGGEFPVHDT ETNQGGLLQV RCDGVAVIFA HNQIFIGLSN
1360 1370 1380 1390 1400
IKKCNTFGGN VFLLEEFDRK KGEIIQRRYF SQMADQICYA VLCVFSLAAA

GHKKEEHSK
Length:1,409
Mass (Da):157,459
Last modified:July 1, 1997 - v1
Checksum:i2DDE6395AC963313
GO
Isoform b (identifier: O02626-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1385-1409: DQICYAVLCVFSLAAAGHKKEEHSK → VDIAWAMHRVFSVQFAISCQKDTN

Note: No experimental confirmation available.
Show »
Length:1,408
Mass (Da):157,478
Checksum:i16BBDA4252C87C29
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1385 – 140925DQICY…EEHSK → VDIAWAMHRVFSVQFAISCQ KDTN in isoform b. CuratedVSP_015555Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93842 mRNA. Translation: AAB52421.1.
FO080298 Genomic DNA. Translation: CCD62710.1.
FO080298 Genomic DNA. Translation: CCD62711.1.
PIRiT37188.
RefSeqiNP_001024342.1. NM_001029171.3. [O02626-2]
NP_741710.1. NM_171618.3. [O02626-1]
UniGeneiCel.18148.

Genome annotation databases

EnsemblMetazoaiC02H7.3a; C02H7.3a; WBGene00000086. [O02626-1]
GeneIDi180420.
KEGGicel:CELE_C02H7.3.
UCSCiC02H7.3a. c. elegans. [O02626-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93842 mRNA. Translation: AAB52421.1.
FO080298 Genomic DNA. Translation: CCD62710.1.
FO080298 Genomic DNA. Translation: CCD62711.1.
PIRiT37188.
RefSeqiNP_001024342.1. NM_001029171.3. [O02626-2]
NP_741710.1. NM_171618.3. [O02626-1]
UniGeneiCel.18148.

3D structure databases

ProteinModelPortaliO02626.
SMRiO02626. Positions 263-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C02H7.3a.

Proteomic databases

EPDiO02626.
PaxDbiO02626.
PRIDEiO02626.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC02H7.3a; C02H7.3a; WBGene00000086. [O02626-1]
GeneIDi180420.
KEGGicel:CELE_C02H7.3.
UCSCiC02H7.3a. c. elegans. [O02626-1]

Organism-specific databases

CTDi180420.
WormBaseiC02H7.3a; CE16806; WBGene00000086; aex-3.
C02H7.3b; CE30850; WBGene00000086; aex-3.

Phylogenomic databases

eggNOGiKOG3570. Eukaryota.
ENOG410XP23. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000231438.
InParanoidiO02626.
OMAiMHSQVFI.
OrthoDBiEOG708VXV.
PhylomeDBiO02626.

Miscellaneous databases

PROiO02626.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "aex-3 encodes a novel regulator of presynaptic activity in C. elegans."
    Iwasaki K., Staunton J., Saifee O., Nonet M., Thomas J.H.
    Neuron 18:613-622(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "The rab3 GDP/GTP exchange factor homolog AEX-3 has a dual function in synaptic transmission."
    Iwasaki K., Toyonaga R.
    EMBO J. 19:4806-4816(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CAB-1.

Entry informationi

Entry nameiMADD_CAEEL
AccessioniPrimary (citable) accession number: O02626
Secondary accession number(s): Q27467, Q8MQF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.