O02611 (TPIS_ENTHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:

TPI

Organism

Entamoeba histolytica

Taxonomic identifier

5759 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Archamoebae › Entamoebidae › Entamoeba

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Subunit structure	Homodimer. Ref.2
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Molecular function	Isomerase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 261

261

Triosephosphate isomerase

PRO_0000090133

Sites

Active site

102

1

Electrophile By similarity

Active site

174

1

Proton acceptor By similarity

Binding site

11

1

Substrate By similarity

Binding site

13

1

Substrate By similarity

Experimental info

Sequence conflict

261

1

L → F in CAA73817. Ref.1

Secondary structure

1

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261

Helix Strand Turn

Details...

Beta strand

6 – 11

6

Helix

18 – 31

14

Helix

34 – 37

4

Beta strand

40 – 46

7

Helix

48 – 50

3

Helix

51 – 59

9

Helix

64 – 66

3

Beta strand

67 – 72

6

Beta strand

75 – 80

6

Helix

87 – 92

6

Beta strand

97 – 101

5

Helix

103 – 108

6

Helix

113 – 126

14

Beta strand

129 – 134

6

Helix

138 – 142

5

Helix

146 – 160

15

Helix

164 – 168

5

Beta strand

169 – 173

5

Helix

176 – 178

3

Beta strand

179 – 182

4

Helix

187 – 204

18

Helix

207 – 212

6

Beta strand

215 – 217

3

Turn

223 – 225

3

Helix

226 – 230

5

Beta strand

237 – 241

5

Helix

242 – 245

4

Helix

247 – 254

8

Helix

255 – 259

5

Sequences

	Sequence		Length	Mass (Da)
	O02611 [UniParc]. Last modified February 12, 2003. Version 2. Checksum: 07CC091A400EFC6B	FASTA	261	27,935
	10 20 30 40 50 60 MGAGKFVVGG NWKCNGTLAS IETLTKGVAA SVDAELAKKV EVIVGVPFIY IPKVQQILAG 70 80 90 100 110 120 EANGANILVS AENAWTKSGA YTGEVHVGML VDCQVPYVIL GHSERRQIFH ESNEQVAEKV 130 140 150 160 170 180 KVAIDAGLKV IACIGETEAQ RIANQTEEVV AAQLKAINNA ISKEAWKNII LAYEPVWAIG 190 200 210 220 230 240 TGKTATPDQA QEVHQYIRKW MTENISKEVA EATRIQYGGS VNPANCNELA KKADIDGFLV 250 260 GGASLDAAKF KTIINSVSEK L

References

[1]	"Sequencing, expression and properties of triosephosphate isomerase from Entamoeba histolytica." Landa A., Rojo-Dominguez A., Jimenez L., Fernandez-Velasco D.A. Eur. J. Biochem. 247:348-355(1997) [PubMed: 9249046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: ATCC 30459 / HM-1:IMSS.
[2]	"Structure and inactivation of triosephosphate isomerase from Entamoeba histolytica." Rodriguez-Romero A., Hernandez-Santoyo A., del Pozo Yauner L., Kornhauser A., Fernandez-Velasco D.A. J. Mol. Biol. 322:669-675(2002) [PubMed: 12270704] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), SEQUENCE REVISION TO 261, HOMODIMERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y13387 mRNA. Translation: CAA73817.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M6J	X-ray	1.50	A/B	1-261	[»]

ProteinModelPortal

O02611.

SMR

O02611. Positions 2-261.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR21139. Triophos_ismrse. 1 hit.

Pfam

PF00121. TIM. 1 hit.
[Graphical view]

SUPFAM

SSF51351. Triophos_ismrse. 1 hit.

TIGRFAMs

TIGR00419. Tim. 1 hit.

PROSITE

PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TPIS_ENTHI

Accession

Primary (citable) accession number: O02611

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	February 12, 2003
Last modified:	October 19, 2011
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)