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Reviewed, UniProtKB/Swiss-Prot O02604 (DRTS_PLAVI)

Last modified June 16, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthase
      Short name=DHFR-TS
Including the following 2 domains:
    1- Recommended name:
            Dihydrofolate reductase
              EC=1.5.1.3
    2- Recommended name:
            Thymidylate synthase
              EC=2.1.1.45
OrganismPlasmodium vivax
Taxonomic identifier5855 [NCBI]
Taxonomic lineageEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Plasmodium)

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.

Domain

The repeat region is missing in the pyrimethamine-resistant isolates BUR-98 and BUR-151.

Miscellaneous

The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.

Sequence similarities

In the N-terminal section; belongs to the dihydrofolate reductase family.

In the C-terminal section; belongs to the thymidylate synthase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 623623Bifunctional dihydrofolate reductase-thymidylate synthase
PRO_0000186351

Regions

Domain9 – 237229DHFR
Repeat88 – 9141
Repeat94 – 9742
Repeat100 – 10343
Region88 – 103163 X 4 AA repeats of G-G-D-N
Region337 – 623287Thymidylate synthase

Sites

Active site5051 By similarity

Natural variations

Natural variant581S → R in the pyrimethamine-resistant isolates BUR-98 and BUR-151.
Natural variant1171S → N in the pyrimethamine-resistant isolates BUR-98 and BUR-151.

Secondary structure

............................................ 623
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O02604-1 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 3E6E958F04FB5828

FASTA62371,057
        10         20         30         40         50         60 
MEDLSDVFDI YAICACCKVA PTSEGTKNEP FSPRTFRGLG NKGTLPWKCN SVDMKYFSSV 

        70         80         90        100        110        120 
TTYVDESKYE KLKWKRERYL RMEASQGGGD NTSGGDNTHG GDNADKLQNV VVMGRSSWES 

       130        140        150        160        170        180 
IPKQYKPLPN RINVVLSKTL TKEDVKEKVF IIDSIDDLLL LLKKLKYYKC FIIGGAQVYR 

       190        200        210        220        230        240 
ECLSRNLIKQ IYFTRINGAY PCDVFFPEFD ESQFRVTSVS EVYNSKGTTL DFLVYSKVGG 

       250        260        270        280        290        300 
GVDGGASNGS TATALRRTAM RSTAMRRNVA PRTAAPPMGP HSRANGERAP PRARARRTTP 

       310        320        330        340        350        360 
RQRKTTSCTS ALTTKWGRKT RSTCKILKFT TASRLMQHPE YQYLGIIYDI IMNGNKQGDR 

       370        380        390        400        410        420 
TGVGVMSNFG YMMKFNLSEY FPLLTTKKLF LRGIIEELLW FIRGETNGNT LLNKNVRIWE 

       430        440        450        460        470        480 
ANGTREFLDN RKLFHREVND LGPIYGFQWR HFGAEYTNMH DNYEDKGVDQ LKNVIHLIKN 

       490        500        510        520        530        540 
EPTSRRIILC AWNVKDLDQM ALPPCHILCQ FYVFDGKLSC IMYQRSCDLG LGVPFNIASY 

       550        560        570        580        590        600 
SIFTHMIAQV CNLQPAQFIH ILGNAHVYNN HVDSLKVQLN RIPYPFPTLK LNPEVKNIED 

       610        620 
FTISDFTIEN YVHHDKITME MAA 

« Hide

References

[1]"Analysis of the Plasmodium vivax dihydrofolate reductase-thymidylate synthase gene sequence."
Eldin de Pecoulas P., Basco L.K., Tahar R., Ouatas T., Mazabraud A.
Gene 211:177-185(1998) [PubMed: 9573357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Isolate Ari/Pakistan, Isolate BUR-151 and Isolate BUR-98.
+Additional computationally mapped references.

Cross-references

Sequence databases

X98123 Genomic DNA. Translation: CAA66805.1.
PIRJC6568.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2BL9X-ray1.90A1-238[»]
2BLAX-ray2.50A1-238[»]
2BLBX-ray3.00A1-238[»]
2BLCX-ray2.25A1-238[»]
SMRO02604. Positions 338-623.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.5.1.3. 257191.
2.1.1.45. 257191.

Family and domain databases

InterProIPR012262. DHFR-TS.
IPR001796. DHFR_reg.
IPR017925. Dihydrofolate_reductase_CS.
IPR000398. Thymidylat_synth_C.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFPIRSF000389. DHFR-TS. 1 hit.
PRINTSPR00108. THYMDSNTHASE.
ProDomPD001180. Thymidylat_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDRTS_PLAVI
AccessionPrimary (citable) accession number: O02604
Secondary accession number(s): O15873
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 30, 2000
Last modified: June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents