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Protein

Synaptobrevin-1

Gene

snb-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Acts in neuronal exocytosis of synaptic transmission. Likely to have a role in cholinergic transmisson. Required for viability, coordinated movement and M3 pharynx motor neuron function.1 Publication

GO - Molecular functioni

GO - Biological processi

  • defecation Source: WormBase
  • nematode larval development Source: WormBase
  • regulation of hindgut contraction Source: WormBase
  • regulation of pharyngeal pumping Source: WormBase
  • synaptic transmission, cholinergic Source: WormBase
  • synaptic vesicle exocytosis Source: WormBase
  • vesicle fusion Source: GO_Central
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-CEL-210500. Glutamate Neurotransmitter Release Cycle.
R-CEL-264642. Acetylcholine Neurotransmitter Release Cycle.
R-CEL-6811440. Retrograde transport at the Trans-Golgi-Network.

Protein family/group databases

TCDBi1.F.1.1.3. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptobrevin-1
Alternative name(s):
Synaptobrevin-related protein 1
Gene namesi
Name:snb-1Imported
ORF Names:T10H9.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT10H9.4; CE18252; WBGene00004897; snb-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8686CytoplasmicSequence analysisAdd
BLAST
Transmembranei87 – 10721Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini108 – 1092ExtracellularSequence analysis

GO - Cellular componenti

  • axon Source: WormBase
  • cell junction Source: UniProtKB-KW
  • cortical granule Source: WormBase
  • cytosol Source: GOC
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: WormBase
  • presynaptic active zone Source: WormBase
  • SNARE complex Source: GO_Central
  • synaptic vesicle Source: WormBase
  • synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Worms exhibit arrested development after embryogenesis. Viable mutants appear lethargic with sporadic jerky movements and absent M3 pharynx motorneuron activity. All mutants are resistant to the acetylcholinesterase inhibitor aldicarb indicating impaired cholinergic transmission.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi62 – 621L → F in js17; resist to aldicarb. 1 Publication
Mutagenesisi66 – 661A → G in js44; resist to aldicarb. 1 Publication
Mutagenesisi97 – 971I → D in e1563; viable showing only slightly increased rate of thrashing behavior. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 109109Synaptobrevin-1PRO_0000307354Add
BLAST

Proteomic databases

EPDiO02495.
PaxDbiO02495.
PRIDEiO02495.

Expressioni

Tissue specificityi

Expressed in the nervous system notably the nerve ring, ventral cord and dorsal cord.1 Publication

Interactioni

Subunit structurei

Part of the SNARE core complex containing ric-4/SNAP25, snb-1/VAMP2 and unc-64/STX1A. This complex binds to cpx-1/CPLX1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-27164N.
STRINGi6239.T10H9.4.

Structurei

3D structure databases

ProteinModelPortaliO02495.
SMRiO02495. Positions 20-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 8361v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Sequence analysis
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
InParanoidiO02495.
KOiK13504.
OMAiMIIIMCA.
OrthoDBiEOG7MSMRJ.
PhylomeDBiO02495.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O02495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAQGDAGAQ GGSQGGPRPS NKRLQQTQAQ VDEVVGIMKV NVEKVLERDQ
60 70 80 90 100
KLSQLDDRAD ALQEGASQFE KSAATLKRKY WWKNIKMMII MCAIVVILII

IIVLWAGGK
Length:109
Mass (Da):12,025
Last modified:July 1, 1997 - v1
Checksum:i3BBC0F085CE070EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003281 mRNA. Translation: AAB61234.1.
AF003282 Genomic DNA. Translation: AAB61235.1.
FO081382 Genomic DNA. Translation: CCD71211.1.
PIRiT33239.
RefSeqiNP_504688.1. NM_072287.4.
UniGeneiCel.5604.

Genome annotation databases

EnsemblMetazoaiT10H9.4; T10H9.4; WBGene00004897.
GeneIDi266648.
KEGGicel:CELE_T10H9.4.
UCSCiT10H9.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003281 mRNA. Translation: AAB61234.1.
AF003282 Genomic DNA. Translation: AAB61235.1.
FO081382 Genomic DNA. Translation: CCD71211.1.
PIRiT33239.
RefSeqiNP_504688.1. NM_072287.4.
UniGeneiCel.5604.

3D structure databases

ProteinModelPortaliO02495.
SMRiO02495. Positions 20-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-27164N.
STRINGi6239.T10H9.4.

Protein family/group databases

TCDBi1.F.1.1.3. the synaptosomal vesicle fusion pore (svf-pore) family.

Proteomic databases

EPDiO02495.
PaxDbiO02495.
PRIDEiO02495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT10H9.4; T10H9.4; WBGene00004897.
GeneIDi266648.
KEGGicel:CELE_T10H9.4.
UCSCiT10H9.4. c. elegans.

Organism-specific databases

CTDi266648.
WormBaseiT10H9.4; CE18252; WBGene00004897; snb-1.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
InParanoidiO02495.
KOiK13504.
OMAiMIIIMCA.
OrthoDBiEOG7MSMRJ.
PhylomeDBiO02495.

Enzyme and pathway databases

ReactomeiR-CEL-210500. Glutamate Neurotransmitter Release Cycle.
R-CEL-264642. Acetylcholine Neurotransmitter Release Cycle.
R-CEL-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiO02495.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Synaptic transmission deficits in Caenorhabditis elegans synaptobrevin mutants."
    Nonet M.L., Saifee O., Zhao H., Rand J.B., Wei L.
    J. Neurosci. 18:70-80(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-62 AND ALA-66, DISRUPTION PHENOTYPE.
    Strain: Bristol N2Imported.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Visualization of synaptic specializations in live C. elegans with synaptic vesicle protein-GFP fusions."
    Nonet M.L.
    J. Neurosci. Methods 89:33-40(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Epileptic-like convulsions associated with LIS-1 in the cytoskeletal control of neurotransmitter signaling in Caenorhabditis elegans."
    Williams S.N., Locke C.J., Braden A.L., Caldwell K.A., Caldwell G.A.
    Hum. Mol. Genet. 13:2043-2059(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "A genetic interaction between the vesicular acetylcholine transporter VAChT/UNC-17 and synaptobrevin/SNB-1 in C. elegans."
    Sandoval G.M., Duerr J.S., Hodgkin J., Rand J.B., Ruvkun G.
    Nat. Neurosci. 9:599-601(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ILE-97.
  6. "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane."
    Dittman J.S., Kaplan J.M.
    Proc. Natl. Acad. Sci. U.S.A. 103:11399-11404(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSYB1_CAEEL
AccessioniPrimary (citable) accession number: O02495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.