O02373 (UGDH_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 6-dehydrogenase
Short name=UDP-Glc dehydrogenase
Short name=UDP-GlcDH
Short name=UDPGDH
EC=1.1.1.22

Alternative name(s):
Protein sugarless
Protein suppenkasper

Gene names

Name:	sgl
Synonyms:	kiwi, ska
ORF Names:	CG10072

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate and heparan sulfate. Required for wingless signaling in different tissues. Ref.1 Ref.2 Ref.3
Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH. Ref.1
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. Ref.1
Disruption phenotype	'Wingless-like' cuticular phenotype; reduced growth of imaginal disks and pattern defects. Ref.1 Ref.3
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-glucuronate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway Wnt receptor signaling pathway Traceable author statement PubMed 12417410. Source: FlyBase chondroitin sulfate biosynthetic process Inferred from mutant phenotype PubMed 10806213. Source: UniProtKB epithelial cell migration, open tracheal system Inferred from mutant phenotype PubMed 10433902. Source: UniProtKB fibroblast growth factor receptor signaling pathway Inferred from mutant phenotype PubMed 10433902. Source: UniProtKB heart development Non-traceable author statement PubMed 12417410. Source: FlyBase heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Inferred from mutant phenotype PubMed 10806213. Source: UniProtKB imaginal disc-derived wing morphogenesis Inferred from mutant phenotype PubMed 21635778. Source: FlyBase inter-male aggressive behavior Inferred from mutant phenotype PubMed 19519879. Source: FlyBase mesoderm migration involved in gastrulation Inferred from mutant phenotype PubMed 10433902. Source: UniProtKB mushroom body development Inferred from mutant phenotype PubMed 19519879. Source: FlyBase olfactory behavior Inferred from mutant phenotype PubMed 17028343. Source: FlyBase primary branching, open tracheal system Traceable author statement PubMed 14570584. Source: FlyBase segment polarity determination Inferred from mutant phenotype PubMed 16049108PubMed 8978055. Source: FlyBase smoothened signaling pathway Traceable author statement PubMed 12417410. Source: FlyBase
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from mutant phenotype PubMed 10806213. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	UDP-glucose 6-dehydrogenase		PRO_0000074064
Regions
	Nucleotide binding	7 – 12	6	NAD By similarity
	Nucleotide binding	85 – 89	5	NAD By similarity
	Nucleotide binding	126 – 127	2	NAD By similarity
	Nucleotide binding	272 – 275	4	NAD By similarity
	Region	157 – 161	5	Substrate binding By similarity
	Region	216 – 223	8	Substrate binding By similarity
	Region	256 – 269	14	Substrate binding By similarity
	Region	334 – 335	2	Substrate binding By similarity
Sites
	Active site	272	1	Nucleophile By similarity
	Binding site	32	1	NAD By similarity
	Binding site	37	1	NAD By similarity
	Binding site	161	1	NAD By similarity
	Binding site	342	1	NAD By similarity
	Binding site	439	1	Substrate By similarity
Experimental info
	Sequence conflict	59	1	K → R in AAC97125. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O02373 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 1450B9A0C802BBE7	FASTA	476	52,875
	10 20 30 40 50 60 MKVCCIGAGY VGGPTCAVMA LKCPDIVITL VDKSSERIAQ WNSDKLPIYE PGLDEVVKKC 70 80 90 100 110 120 RNVNLFFSTD IETAIKEADL IFISVNTPTK TCGNGKGRAA DLKYVESAAR MIAEIAQSNK 130 140 150 160 170 180 IVVEKSTVPV RAAESIMHIL RANQKPGIHY DILSNPEFLA EGTAINDLLN ADRVLIGGEE 190 200 210 220 230 240 TPEGHQAVEK LSWIYEHWIP KQNILTTNTW SSELSKLAAN AFLAQRISSI NSLSAVCEAT 250 260 270 280 290 300 GADVSEVARA VGLDSRIGSK FLQASVGFGG SCFQKDILNL IYICENLNLP EVAAYWQQVI 310 320 330 340 350 360 DMNEYQKRRF SQKIIESLFN TVSDKRIAIL GFAFKKNTGD TRETAAITVC QTLLEEGAAL 370 380 390 400 410 420 DIYDPKVEPE QIIDDLTHPS VTESPEKVKK AVQIHSDPYS AVRATHALVI CTEWDEFVDL 430 440 450 460 470 DFKRIYQSMM KPAYIFDGRK ILDHERLQQI GFHVQTIGKK YQRTGLLRSW GIVPQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic evidence that heparin-like glycosaminoglycans are involved in wingless signaling." Binari R.C., Staveley B.E., Johnson W.A., Godavarti R., Sasisekharan R., Manoukian A.S. Development 124:2623-2632(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, DISRUPTION PHENOTYPE.
[2]	"The Drosophila sugarless gene modulates Wingless signaling and encodes an enzyme involved in polysaccharide biosynthesis." Haecker U., Lin X., Perrimon N. Development 124:3565-3573(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]	"Defects in glucuronate biosynthesis disrupt Wingless signaling in Drosophila." Haerry T.E., Heslip T.R., Marsh J.L., O'Connor M.B. Development 124:3055-3064(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
[4]	"Mutation in the Drosophila gene encoding UDP-glucose dehydrogenase affects expression of unlinked genes." Benevolenskaya E.V., Frolov M.V., Birchler J.A. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-76. Strain: Canton-S.
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[7]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF007870 mRNA. Translation: AAB63208.1. AF000570 mRNA. Translation: AAB58714.1. AF009013 mRNA. Translation: AAB63462.1. AF001310 mRNA. Translation: AAC97125.1. AH007072 Genomic DNA. Translation: AAC97126.1. AE014296 Genomic DNA. Translation: AAF50631.1. AY052137 mRNA. Translation: AAK93561.1.
RefSeq	NP_476980.1. NM_057632.4.
UniGene	Dm.4371.
3D structure databases
ProteinModelPortal	O02373.
SMR	O02373. Positions 2-460.
ModBase	Search...
Proteomic databases
PaxDb	O02373.
PRIDE	O02373.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0076938; FBpp0076647; FBgn0261445.
GeneID	38760.
KEGG	dme:Dmel_CG10072.
Organism-specific databases
CTD	24054.
FlyBase	FBgn0261445. sgl.
Phylogenomic databases
eggNOG	COG1004.
GeneTree	ENSGT00390000015355.
InParanoid	O02373.
KO	K00012.
OMA	QMCPEIR.
OrthoDB	EOG4STQKV.
PhylomeDB	O02373.
Enzyme and pathway databases
UniPathway	UPA00038; UER00491.
Gene expression databases
Bgee	O02373.
GermOnline	CG10072. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMART	SM00984. UDPG_MGDP_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit. SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	38760.
NextBio	810251.

Entry information

Entry name

UGDH_DROME

Accession

Primary (citable) accession number: O02373
Secondary accession number(s): O02647, Q9VRZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project