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O02373

- UGDH_DROME

UniProt

O02373 - UGDH_DROME

Protein

UDP-glucose 6-dehydrogenase

Gene

sgl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate and heparan sulfate. Required for wingless signaling in different tissues.3 Publications

    Catalytic activityi

    UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321NADBy similarity
    Binding sitei37 – 371NADBy similarity
    Binding sitei161 – 1611NADBy similarity
    Active sitei272 – 2721NucleophileBy similarity
    Binding sitei342 – 3421NADBy similarity
    Binding sitei439 – 4391SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 126NADBy similarity
    Nucleotide bindingi85 – 895NADBy similarity
    Nucleotide bindingi126 – 1272NADBy similarity
    Nucleotide bindingi272 – 2754NADBy similarity

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. UDP-glucose 6-dehydrogenase activity Source: UniProtKB

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: FlyBase
    2. chondroitin sulfate biosynthetic process Source: UniProtKB
    3. epithelial cell migration, open tracheal system Source: UniProtKB
    4. fibroblast growth factor receptor signaling pathway Source: UniProtKB
    5. glycosaminoglycan biosynthetic process Source: UniProtKB
    6. heart development Source: FlyBase
    7. heparan sulfate proteoglycan biosynthetic process Source: FlyBase
    8. heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
    9. imaginal disc-derived wing morphogenesis Source: FlyBase
    10. inter-male aggressive behavior Source: FlyBase
    11. mesoderm migration involved in gastrulation Source: UniProtKB
    12. mushroom body development Source: FlyBase
    13. olfactory behavior Source: FlyBase
    14. primary branching, open tracheal system Source: FlyBase
    15. segment polarity determination Source: FlyBase
    16. smoothened signaling pathway Source: FlyBase
    17. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway
    18. Wnt signaling pathway Source: FlyBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Wnt signaling pathway

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_203410. Formation of the active cofactor, UDP-glucuronate.
    UniPathwayiUPA00038; UER00491.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
    Short name:
    UDP-Glc dehydrogenase
    Short name:
    UDP-GlcDH
    Short name:
    UDPGDH
    Alternative name(s):
    Protein sugarless
    Protein suppenkasper
    Gene namesi
    Name:sgl
    Synonyms:kiwi, ska
    ORF Names:CG10072
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0261445. sgl.

    Pathology & Biotechi

    Disruption phenotypei

    'Wingless-like' cuticular phenotype; reduced growth of imaginal disks and pattern defects.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 476476UDP-glucose 6-dehydrogenasePRO_0000074064Add
    BLAST

    Proteomic databases

    PaxDbiO02373.
    PRIDEiO02373.

    Expressioni

    Gene expression databases

    BgeeiO02373.

    Interactioni

    Protein-protein interaction databases

    BioGridi64209. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO02373.
    SMRiO02373. Positions 2-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni157 – 1615Substrate bindingBy similarity
    Regioni216 – 2238Substrate bindingBy similarity
    Regioni256 – 26914Substrate bindingBy similarityAdd
    BLAST
    Regioni334 – 3352Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1004.
    GeneTreeiENSGT00390000015355.
    InParanoidiO02373.
    KOiK00012.
    OMAiVELNTWQ.
    OrthoDBiEOG7034GP.
    PhylomeDBiO02373.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028356. UDPglc_DH_euk.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PTHR11374:SF3. PTHR11374:SF3. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O02373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVCCIGAGY VGGPTCAVMA LKCPDIVITL VDKSSERIAQ WNSDKLPIYE    50
    PGLDEVVKKC RNVNLFFSTD IETAIKEADL IFISVNTPTK TCGNGKGRAA 100
    DLKYVESAAR MIAEIAQSNK IVVEKSTVPV RAAESIMHIL RANQKPGIHY 150
    DILSNPEFLA EGTAINDLLN ADRVLIGGEE TPEGHQAVEK LSWIYEHWIP 200
    KQNILTTNTW SSELSKLAAN AFLAQRISSI NSLSAVCEAT GADVSEVARA 250
    VGLDSRIGSK FLQASVGFGG SCFQKDILNL IYICENLNLP EVAAYWQQVI 300
    DMNEYQKRRF SQKIIESLFN TVSDKRIAIL GFAFKKNTGD TRETAAITVC 350
    QTLLEEGAAL DIYDPKVEPE QIIDDLTHPS VTESPEKVKK AVQIHSDPYS 400
    AVRATHALVI CTEWDEFVDL DFKRIYQSMM KPAYIFDGRK ILDHERLQQI 450
    GFHVQTIGKK YQRTGLLRSW GIVPQL 476
    Length:476
    Mass (Da):52,875
    Last modified:July 1, 1997 - v1
    Checksum:i1450B9A0C802BBE7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti59 – 591K → R in AAC97125. (PubMed:9272947)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007870 mRNA. Translation: AAB63208.1.
    AF000570 mRNA. Translation: AAB58714.1.
    AF009013 mRNA. Translation: AAB63462.1.
    AF001310 mRNA. Translation: AAC97125.1.
    AH007072 Genomic DNA. Translation: AAC97126.1.
    AE014296 Genomic DNA. Translation: AAF50631.1.
    AY052137 mRNA. Translation: AAK93561.1.
    RefSeqiNP_476980.1. NM_057632.4.
    UniGeneiDm.4371.

    Genome annotation databases

    EnsemblMetazoaiFBtr0076938; FBpp0076647; FBgn0261445.
    GeneIDi38760.
    KEGGidme:Dmel_CG10072.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007870 mRNA. Translation: AAB63208.1 .
    AF000570 mRNA. Translation: AAB58714.1 .
    AF009013 mRNA. Translation: AAB63462.1 .
    AF001310 mRNA. Translation: AAC97125.1 .
    AH007072 Genomic DNA. Translation: AAC97126.1 .
    AE014296 Genomic DNA. Translation: AAF50631.1 .
    AY052137 mRNA. Translation: AAK93561.1 .
    RefSeqi NP_476980.1. NM_057632.4.
    UniGenei Dm.4371.

    3D structure databases

    ProteinModelPortali O02373.
    SMRi O02373. Positions 2-460.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 64209. 9 interactions.

    Proteomic databases

    PaxDbi O02373.
    PRIDEi O02373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0076938 ; FBpp0076647 ; FBgn0261445 .
    GeneIDi 38760.
    KEGGi dme:Dmel_CG10072.

    Organism-specific databases

    CTDi 24054.
    FlyBasei FBgn0261445. sgl.

    Phylogenomic databases

    eggNOGi COG1004.
    GeneTreei ENSGT00390000015355.
    InParanoidi O02373.
    KOi K00012.
    OMAi VELNTWQ.
    OrthoDBi EOG7034GP.
    PhylomeDBi O02373.

    Enzyme and pathway databases

    UniPathwayi UPA00038 ; UER00491 .
    Reactomei REACT_203410. Formation of the active cofactor, UDP-glucuronate.

    Miscellaneous databases

    GenomeRNAii 38760.
    NextBioi 810251.
    PROi O02373.

    Gene expression databases

    Bgeei O02373.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028356. UDPglc_DH_euk.
    [Graphical view ]
    PANTHERi PTHR11374. PTHR11374. 1 hit.
    PTHR11374:SF3. PTHR11374:SF3. 1 hit.
    Pfami PF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500133. UDPglc_DH_euk. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic evidence that heparin-like glycosaminoglycans are involved in wingless signaling."
      Binari R.C., Staveley B.E., Johnson W.A., Godavarti R., Sasisekharan R., Manoukian A.S.
      Development 124:2623-2632(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY, DISRUPTION PHENOTYPE.
    2. "The Drosophila sugarless gene modulates Wingless signaling and encodes an enzyme involved in polysaccharide biosynthesis."
      Haecker U., Lin X., Perrimon N.
      Development 124:3565-3573(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    3. "Defects in glucuronate biosynthesis disrupt Wingless signaling in Drosophila."
      Haerry T.E., Heslip T.R., Marsh J.L., O'Connor M.B.
      Development 124:3055-3064(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE.
    4. "Mutation in the Drosophila gene encoding UDP-glucose dehydrogenase affects expression of unlinked genes."
      Benevolenskaya E.V., Frolov M.V., Birchler J.A.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-76.
      Strain: Canton-S.
    5. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    6. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.

    Entry informationi

    Entry nameiUGDH_DROME
    AccessioniPrimary (citable) accession number: O02373
    Secondary accession number(s): O02647, Q9VRZ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3