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Protein

Tyramine receptor Ser-2

Gene

ser-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for tyramine, a known neurotransmitter and neuromodulator and direct precursor of octopamine. The rank order of potency is tyramine > octopamine > dopamine > serotonin > epinephrine = norepinephrine.1 Publication

GO - Molecular functioni

  • octopamine receptor activity Source: InterPro
  • tyramine receptor activity Source: UniProtKB

GO - Biological processi

  • dopamine receptor signaling pathway Source: WormBase
  • octopamine or tyramine signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-CEL-390666. Serotonin receptors.
R-CEL-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyramine receptor Ser-2
Gene namesi
Name:ser-2
ORF Names:C02D4.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiC02D4.2a; CE36088; WBGene00004777; ser-2.
C02D4.2b; CE36089; WBGene00004777; ser-2.
C02D4.2c; CE36423; WBGene00004777; ser-2.
C02D4.2d; CE36424; WBGene00004777; ser-2.
C02D4.2e; CE36092; WBGene00004777; ser-2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6060ExtracellularSequence analysisAdd
BLAST
Transmembranei61 – 8323Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini84 – 9310CytoplasmicSequence analysis
Transmembranei94 – 11522Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini116 – 13318ExtracellularSequence analysisAdd
BLAST
Transmembranei134 – 15421Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini155 – 17420CytoplasmicSequence analysisAdd
BLAST
Transmembranei175 – 19723Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini198 – 22124ExtracellularSequence analysisAdd
BLAST
Transmembranei222 – 24322Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini244 – 370127CytoplasmicSequence analysisAdd
BLAST
Transmembranei371 – 39222Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini393 – 40715ExtracellularSequence analysisAdd
BLAST
Transmembranei408 – 42821Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini429 – 45527CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

A loss-of-function mutation does not affect the development of AIY interneurons, or lead to egg-laying or any other behavioral defects.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi155 – 1551D → A: No obvious behavioral defects. 1 Publication
Mutagenesisi368 – 3681A → E: No obvious behavioral defects. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455Tyramine receptor Ser-2PRO_0000070108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence analysis
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi131 ↔ 209PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO02213.

Expressioni

Tissue specificityi

The different isoforms are expressed in specific, but overlapping sets of sensory, inter- and motor neurons, including AIY, AIZ and RIA interneurons. They are also expressed in pharyngeal cells, head muscles and excretory gland cells.1 Publication

Gene expression databases

ExpressionAtlasiO02213. baseline.

Interactioni

Protein-protein interaction databases

STRINGi6239.C02D4.2e.

Structurei

3D structure databases

ProteinModelPortaliO02213.
SMRiO02213. Positions 58-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
InParanoidiO02213.
OMAiSALMRIQ.
OrthoDBiEOG7NCV3Q.
PhylomeDBiO02213.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002002. Octopmn_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00664. OCTOPAMINER.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform e (identifier: O02213-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRNYTDSVQ EMVLRAIDSI RDSVINASSA VSTTTLPPLD IPMTSMKPPS
60 70 80 90 100
IIPTVELVLG TITYLVIIAM TVVGNTLVVV AVFSYRPLKK VQNYFLVSLA
110 120 130 140 150
ASDLAVAIFV MPLHVVTFLA GGKWLLGVTV CQFFTTADIL LCTSSILNLC
160 170 180 190 200
AIALDRYWAI HNPINYAQKR TTKFVCIVIV IVWILSMLIS VPPIIGWNNW
210 220 230 240 250
QENMMEDSCG LSTEKAFVVF SAAGSFFLPL LVMVVVYVKI FISARQRIRT
260 270 280 290 300
NRGRSALMRI QNAEGDDDYR KMSIKRASVE SARTSSRVGE KTPLVIADGQ
310 320 330 340 350
TTVTTLAAHS TDGGSLPKDE TTKHMKYHNN GSCKVKVKDV KEDEGNPNPT
360 370 380 390 400
AVLRKREKIS VAKEKRAAKT IAVIIFVFSF CWLPFFVAYV IRPFCETCKL
410 420 430 440 450
HAKVEQAFTW LGYINSSLNP FLYGILNLEF RRAFKKILCP KAVLEQRRRR

MSAQP
Note: Produced by alternative promoter usage.
Length:455
Mass (Da):50,870
Last modified:October 25, 2004 - v4
Checksum:iA890DB8A9101E29F
GO
Isoform a (identifier: O02213-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     288-310: Missing.

Note: Produced by alternative splicing of isoform e.
Show »
Length:432
Mass (Da):48,578
Checksum:i164CB439D78CF888
GO
Isoform b (identifier: O02213-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MFRNYTDSV → MNDDFS
     288-310: Missing.

Note: Produced by alternative splicing of isoform g.
Show »
Length:429
Mass (Da):48,174
Checksum:i4001BF233A7F001D
GO
Isoform c (identifier: O02213-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MFRNYTDSV → MNDDFS
     182-202: VWILSMLISVPPIIGWNNWQE → SLQSNYGEGKRRRLPSFVMEM
     203-455: Missing.

Note: Produced by alternative splicing of isoform g.
Show »
Length:199
Mass (Da):21,921
Checksum:iF76D7A9915F4845F
GO
Isoform d (identifier: O02213-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MFRNYTDSVQ → MSSL
     182-202: VWILSMLISVPPIIGWNNWQE → SLQSNYGEGKRRRLPSFVMEM
     203-455: Missing.

Note: Produced by alternative promoter usage.
Show »
Length:196
Mass (Da):21,502
Checksum:i68F365B82505633E
GO
Isoform g (identifier: O02213-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MFRNYTDSV → MNDDFS

Note: Produced by alternative promoter usage. No experimental confirmation available.
Show »
Length:452
Mass (Da):50,465
Checksum:iF30D4DE82952E2E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711T → A in AAN16459 (PubMed:12354282).Curated
Sequence conflicti268 – 2681D → V in AAN16459 (PubMed:12354282).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010MFRNYTDSVQ → MSSL in isoform d. CuratedVSP_011788
Alternative sequencei1 – 99MFRNYTDSV → MNDDFS in isoform b, isoform c and isoform g. CuratedVSP_011787
Alternative sequencei182 – 20221VWILS…NNWQE → SLQSNYGEGKRRRLPSFVME M in isoform c and isoform d. CuratedVSP_011789Add
BLAST
Alternative sequencei203 – 455253Missing in isoform c and isoform d. CuratedVSP_011790Add
BLAST
Alternative sequencei288 – 31023Missing in isoform a and isoform b. CuratedVSP_001920Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81031 Genomic DNA. Translation: CAB02718.4.
Z81031 Genomic DNA. Translation: CAF31464.1.
Z81031 Genomic DNA. Translation: CAF31465.2.
Z81031 Genomic DNA. Translation: CAF31466.2.
Z81031 Genomic DNA. Translation: CAF31467.1.
AF539813 mRNA. Translation: AAN16458.1.
AF539814 mRNA. Translation: AAN16459.1.
PIRiT18863.
RefSeqiNP_001024335.1. NM_001029164.1. [O02213-1]
NP_001024336.1. NM_001029165.1. [O02213-2]
NP_001024337.1. NM_001029166.1. [O02213-3]
NP_001024338.1. NM_001029167.1. [O02213-4]
NP_001024339.1. NM_001029168.1. [O02213-5]
UniGeneiCel.9281.

Genome annotation databases

EnsemblMetazoaiC02D4.2e; C02D4.2e; WBGene00004777. [O02213-5]
GeneIDi182110.
KEGGicel:CELE_C02D4.2.
UCSCiC02D4.2f. c. elegans. [O02213-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81031 Genomic DNA. Translation: CAB02718.4.
Z81031 Genomic DNA. Translation: CAF31464.1.
Z81031 Genomic DNA. Translation: CAF31465.2.
Z81031 Genomic DNA. Translation: CAF31466.2.
Z81031 Genomic DNA. Translation: CAF31467.1.
AF539813 mRNA. Translation: AAN16458.1.
AF539814 mRNA. Translation: AAN16459.1.
PIRiT18863.
RefSeqiNP_001024335.1. NM_001029164.1. [O02213-1]
NP_001024336.1. NM_001029165.1. [O02213-2]
NP_001024337.1. NM_001029166.1. [O02213-3]
NP_001024338.1. NM_001029167.1. [O02213-4]
NP_001024339.1. NM_001029168.1. [O02213-5]
UniGeneiCel.9281.

3D structure databases

ProteinModelPortaliO02213.
SMRiO02213. Positions 58-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C02D4.2e.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiO02213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC02D4.2e; C02D4.2e; WBGene00004777. [O02213-5]
GeneIDi182110.
KEGGicel:CELE_C02D4.2.
UCSCiC02D4.2f. c. elegans. [O02213-1]

Organism-specific databases

CTDi182110.
WormBaseiC02D4.2a; CE36088; WBGene00004777; ser-2.
C02D4.2b; CE36089; WBGene00004777; ser-2.
C02D4.2c; CE36423; WBGene00004777; ser-2.
C02D4.2d; CE36424; WBGene00004777; ser-2.
C02D4.2e; CE36092; WBGene00004777; ser-2.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
InParanoidiO02213.
OMAiSALMRIQ.
OrthoDBiEOG7NCV3Q.
PhylomeDBiO02213.

Enzyme and pathway databases

ReactomeiR-CEL-390666. Serotonin receptors.
R-CEL-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiO02213.

Gene expression databases

ExpressionAtlasiO02213. baseline.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002002. Octopmn_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00664. OCTOPAMINER.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "Characterization of a tyramine receptor from Caenorhabditis elegans."
    Rex E., Komuniecki R.W.
    J. Neurochem. 82:1352-1359(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-455 (ISOFORMS A/B AND E/G), FUNCTION, ALTERNATIVE SPLICING.
  3. "LIM homeobox gene-dependent expression of biogenic amine receptors in restricted regions of the C. elegans nervous system."
    Tsalik E.L., Niacaris T., Wenick A.S., Pau K., Avery L., Hobert O.
    Dev. Biol. 263:81-102(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE PROMOTER USAGE, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-155 AND ALA-368, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSER2_CAEEL
AccessioniPrimary (citable) accession number: O02213
Secondary accession number(s): Q6LA87
, Q6LA88, Q6LA89, Q6LA90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 25, 2004
Last modified: June 8, 2016
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.